Job ID = 6627260 SRX = SRX8521331 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T01:42:20 prefetch.2.10.7: 1) Downloading 'SRR11977993'... 2020-07-14T01:42:20 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:49:43 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:49:43 prefetch.2.10.7: 1) 'SRR11977993' was downloaded successfully 2020-07-14T01:49:43 prefetch.2.10.7: 'SRR11977993' has 0 unresolved dependencies Read 66701753 spots for SRR11977993/SRR11977993.sra Written 66701753 spots for SRR11977993/SRR11977993.sra 2020-07-14T01:54:06 prefetch.2.10.7: 1) Downloading 'SRR11977994'... 2020-07-14T01:54:06 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:07:40 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:07:40 prefetch.2.10.7: 1) 'SRR11977994' was downloaded successfully 2020-07-14T02:07:40 prefetch.2.10.7: 'SRR11977994' has 0 unresolved dependencies Read 63503301 spots for SRR11977994/SRR11977994.sra Written 63503301 spots for SRR11977994/SRR11977994.sra 2020-07-14T02:11:24 prefetch.2.10.7: 1) Downloading 'SRR11977995'... 2020-07-14T02:11:24 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:35:06 prefetch.2.10.7: HTTPS download failed 2020-07-14T02:35:06 prefetch.2.10.7: 1) failed to download SRR11977995 2020-07-14T02:35:20 prefetch.2.10.7: 1) Downloading 'SRR11977995'... 2020-07-14T02:35:20 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:35:20 prefetch.2.10.7: Continue download of 'SRR11977995' from 2427288131 2020-07-14T02:35:25 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:35:25 prefetch.2.10.7: 1) 'SRR11977995' was downloaded successfully 2020-07-14T02:35:25 prefetch.2.10.7: 'SRR11977995' has 0 unresolved dependencies Read 70090097 spots for SRR11977995/SRR11977995.sra Written 70090097 spots for SRR11977995/SRR11977995.sra 2020-07-14T02:39:34 prefetch.2.10.7: 1) Downloading 'SRR11977996'... 2020-07-14T02:39:34 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:49:15 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:49:15 prefetch.2.10.7: 1) 'SRR11977996' was downloaded successfully 2020-07-14T02:49:15 prefetch.2.10.7: 'SRR11977996' has 0 unresolved dependencies Read 69459433 spots for SRR11977996/SRR11977996.sra Written 69459433 spots for SRR11977996/SRR11977996.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627873 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:01:24 269754584 reads; of these: 269754584 (100.00%) were unpaired; of these: 220803369 (81.85%) aligned 0 times 27677761 (10.26%) aligned exactly 1 time 21273454 (7.89%) aligned >1 times 18.15% overall alignment rate Time searching: 01:01:24 Overall time: 01:01:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 22735215 / 48951215 = 0.4644 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 13:11:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 13:11:15: #1 read tag files... INFO @ Tue, 14 Jul 2020 13:11:15: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 13:11:21: 1000000 INFO @ Tue, 14 Jul 2020 13:11:27: 2000000 INFO @ Tue, 14 Jul 2020 13:11:33: 3000000 INFO @ Tue, 14 Jul 2020 13:11:39: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 13:11:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 13:11:45: #1 read tag files... INFO @ Tue, 14 Jul 2020 13:11:45: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 13:11:46: 5000000 INFO @ Tue, 14 Jul 2020 13:11:53: 6000000 INFO @ Tue, 14 Jul 2020 13:11:53: 1000000 INFO @ Tue, 14 Jul 2020 13:12:00: 7000000 INFO @ Tue, 14 Jul 2020 13:12:01: 2000000 INFO @ Tue, 14 Jul 2020 13:12:07: 8000000 INFO @ Tue, 14 Jul 2020 13:12:10: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 13:12:14: 9000000 INFO @ Tue, 14 Jul 2020 13:12:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 13:12:15: #1 read tag files... INFO @ Tue, 14 Jul 2020 13:12:15: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 13:12:18: 4000000 INFO @ Tue, 14 Jul 2020 13:12:21: 10000000 INFO @ Tue, 14 Jul 2020 13:12:22: 1000000 INFO @ Tue, 14 Jul 2020 13:12:27: 5000000 INFO @ Tue, 14 Jul 2020 13:12:29: 11000000 INFO @ Tue, 14 Jul 2020 13:12:30: 2000000 INFO @ Tue, 14 Jul 2020 13:12:35: 6000000 INFO @ Tue, 14 Jul 2020 13:12:36: 12000000 INFO @ Tue, 14 Jul 2020 13:12:37: 3000000 INFO @ Tue, 14 Jul 2020 13:12:43: 7000000 INFO @ Tue, 14 Jul 2020 13:12:43: 13000000 INFO @ Tue, 14 Jul 2020 13:12:44: 4000000 INFO @ Tue, 14 Jul 2020 13:12:50: 14000000 INFO @ Tue, 14 Jul 2020 13:12:51: 8000000 INFO @ Tue, 14 Jul 2020 13:12:52: 5000000 INFO @ Tue, 14 Jul 2020 13:12:58: 15000000 INFO @ Tue, 14 Jul 2020 13:12:59: 9000000 INFO @ Tue, 14 Jul 2020 13:12:59: 6000000 INFO @ Tue, 14 Jul 2020 13:13:05: 16000000 INFO @ Tue, 14 Jul 2020 13:13:07: 7000000 INFO @ Tue, 14 Jul 2020 13:13:07: 10000000 INFO @ Tue, 14 Jul 2020 13:13:12: 17000000 INFO @ Tue, 14 Jul 2020 13:13:14: 8000000 INFO @ Tue, 14 Jul 2020 13:13:15: 11000000 INFO @ Tue, 14 Jul 2020 13:13:20: 18000000 INFO @ Tue, 14 Jul 2020 13:13:21: 9000000 INFO @ Tue, 14 Jul 2020 13:13:23: 12000000 INFO @ Tue, 14 Jul 2020 13:13:27: 19000000 INFO @ Tue, 14 Jul 2020 13:13:29: 10000000 INFO @ Tue, 14 Jul 2020 13:13:31: 13000000 INFO @ Tue, 14 Jul 2020 13:13:34: 20000000 INFO @ Tue, 14 Jul 2020 13:13:36: 11000000 INFO @ Tue, 14 Jul 2020 13:13:39: 14000000 INFO @ Tue, 14 Jul 2020 13:13:42: 21000000 INFO @ Tue, 14 Jul 2020 13:13:43: 12000000 INFO @ Tue, 14 Jul 2020 13:13:47: 15000000 INFO @ Tue, 14 Jul 2020 13:13:49: 22000000 INFO @ Tue, 14 Jul 2020 13:13:51: 13000000 INFO @ Tue, 14 Jul 2020 13:13:55: 16000000 INFO @ Tue, 14 Jul 2020 13:13:57: 23000000 INFO @ Tue, 14 Jul 2020 13:13:58: 14000000 INFO @ Tue, 14 Jul 2020 13:14:02: 17000000 INFO @ Tue, 14 Jul 2020 13:14:04: 24000000 INFO @ Tue, 14 Jul 2020 13:14:05: 15000000 INFO @ Tue, 14 Jul 2020 13:14:10: 18000000 INFO @ Tue, 14 Jul 2020 13:14:11: 25000000 INFO @ Tue, 14 Jul 2020 13:14:12: 16000000 INFO @ Tue, 14 Jul 2020 13:14:18: 19000000 INFO @ Tue, 14 Jul 2020 13:14:18: 26000000 INFO @ Tue, 14 Jul 2020 13:14:19: 17000000 INFO @ Tue, 14 Jul 2020 13:14:20: #1 tag size is determined as 69 bps INFO @ Tue, 14 Jul 2020 13:14:20: #1 tag size = 69 INFO @ Tue, 14 Jul 2020 13:14:20: #1 total tags in treatment: 26216000 INFO @ Tue, 14 Jul 2020 13:14:20: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 13:14:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 13:14:20: #1 tags after filtering in treatment: 26216000 INFO @ Tue, 14 Jul 2020 13:14:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 13:14:20: #1 finished! INFO @ Tue, 14 Jul 2020 13:14:20: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 13:14:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 13:14:22: #2 number of paired peaks: 6 WARNING @ Tue, 14 Jul 2020 13:14:22: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 13:14:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 13:14:26: 20000000 INFO @ Tue, 14 Jul 2020 13:14:26: 18000000 INFO @ Tue, 14 Jul 2020 13:14:33: 19000000 INFO @ Tue, 14 Jul 2020 13:14:33: 21000000 INFO @ Tue, 14 Jul 2020 13:14:40: 20000000 INFO @ Tue, 14 Jul 2020 13:14:41: 22000000 INFO @ Tue, 14 Jul 2020 13:14:47: 21000000 INFO @ Tue, 14 Jul 2020 13:14:49: 23000000 INFO @ Tue, 14 Jul 2020 13:14:54: 22000000 INFO @ Tue, 14 Jul 2020 13:14:57: 24000000 INFO @ Tue, 14 Jul 2020 13:15:01: 23000000 INFO @ Tue, 14 Jul 2020 13:15:04: 25000000 INFO @ Tue, 14 Jul 2020 13:15:08: 24000000 INFO @ Tue, 14 Jul 2020 13:15:12: 26000000 INFO @ Tue, 14 Jul 2020 13:15:14: #1 tag size is determined as 69 bps INFO @ Tue, 14 Jul 2020 13:15:14: #1 tag size = 69 INFO @ Tue, 14 Jul 2020 13:15:14: #1 total tags in treatment: 26216000 INFO @ Tue, 14 Jul 2020 13:15:14: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 13:15:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 13:15:14: #1 tags after filtering in treatment: 26216000 INFO @ Tue, 14 Jul 2020 13:15:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 13:15:14: #1 finished! INFO @ Tue, 14 Jul 2020 13:15:14: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 13:15:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 13:15:15: 25000000 INFO @ Tue, 14 Jul 2020 13:15:16: #2 number of paired peaks: 6 WARNING @ Tue, 14 Jul 2020 13:15:16: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 13:15:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 13:15:22: 26000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 13:15:24: #1 tag size is determined as 69 bps INFO @ Tue, 14 Jul 2020 13:15:24: #1 tag size = 69 INFO @ Tue, 14 Jul 2020 13:15:24: #1 total tags in treatment: 26216000 INFO @ Tue, 14 Jul 2020 13:15:24: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 13:15:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 13:15:24: #1 tags after filtering in treatment: 26216000 INFO @ Tue, 14 Jul 2020 13:15:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 13:15:24: #1 finished! INFO @ Tue, 14 Jul 2020 13:15:24: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 13:15:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 13:15:26: #2 number of paired peaks: 6 WARNING @ Tue, 14 Jul 2020 13:15:26: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 13:15:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521331/SRX8521331.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling