Job ID = 6627234 SRX = SRX8521319 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T01:34:46 prefetch.2.10.7: 1) Downloading 'SRR11977925'... 2020-07-14T01:34:46 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:35:09 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:35:09 prefetch.2.10.7: 'SRR11977925' is valid 2020-07-14T01:35:09 prefetch.2.10.7: 1) 'SRR11977925' was downloaded successfully 2020-07-14T01:35:09 prefetch.2.10.7: 'SRR11977925' has 0 unresolved dependencies Read 933825 spots for SRR11977925/SRR11977925.sra Written 933825 spots for SRR11977925/SRR11977925.sra 2020-07-14T01:35:27 prefetch.2.10.7: 1) Downloading 'SRR11977926'... 2020-07-14T01:35:27 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:35:51 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:35:51 prefetch.2.10.7: 'SRR11977926' is valid 2020-07-14T01:35:51 prefetch.2.10.7: 1) 'SRR11977926' was downloaded successfully 2020-07-14T01:35:51 prefetch.2.10.7: 'SRR11977926' has 0 unresolved dependencies Read 926245 spots for SRR11977926/SRR11977926.sra Written 926245 spots for SRR11977926/SRR11977926.sra 2020-07-14T01:36:09 prefetch.2.10.7: 1) Downloading 'SRR11977927'... 2020-07-14T01:36:09 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:36:30 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:36:30 prefetch.2.10.7: 'SRR11977927' is valid 2020-07-14T01:36:30 prefetch.2.10.7: 1) 'SRR11977927' was downloaded successfully 2020-07-14T01:36:30 prefetch.2.10.7: 'SRR11977927' has 0 unresolved dependencies Read 942978 spots for SRR11977927/SRR11977927.sra Written 942978 spots for SRR11977927/SRR11977927.sra 2020-07-14T01:36:48 prefetch.2.10.7: 1) Downloading 'SRR11977928'... 2020-07-14T01:36:48 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:37:05 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:37:05 prefetch.2.10.7: 'SRR11977928' is valid 2020-07-14T01:37:05 prefetch.2.10.7: 1) 'SRR11977928' was downloaded successfully 2020-07-14T01:37:05 prefetch.2.10.7: 'SRR11977928' has 0 unresolved dependencies Read 926047 spots for SRR11977928/SRR11977928.sra Written 926047 spots for SRR11977928/SRR11977928.sra 2020-07-14T01:37:23 prefetch.2.10.7: 1) Downloading 'SRR11977929'... 2020-07-14T01:37:23 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:37:36 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:37:36 prefetch.2.10.7: 'SRR11977929' is valid 2020-07-14T01:37:36 prefetch.2.10.7: 1) 'SRR11977929' was downloaded successfully 2020-07-14T01:37:36 prefetch.2.10.7: 'SRR11977929' has 0 unresolved dependencies Read 7916 spots for SRR11977929/SRR11977929.sra Written 7916 spots for SRR11977929/SRR11977929.sra 2020-07-14T01:37:50 prefetch.2.10.7: 1) Downloading 'SRR11977930'... 2020-07-14T01:37:50 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:37:59 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:37:59 prefetch.2.10.7: 'SRR11977930' is valid 2020-07-14T01:37:59 prefetch.2.10.7: 1) 'SRR11977930' was downloaded successfully 2020-07-14T01:37:59 prefetch.2.10.7: 'SRR11977930' has 0 unresolved dependencies Read 8703 spots for SRR11977930/SRR11977930.sra Written 8703 spots for SRR11977930/SRR11977930.sra 2020-07-14T01:38:09 prefetch.2.10.7: 1) Downloading 'SRR11977931'... 2020-07-14T01:38:09 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:38:24 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:38:24 prefetch.2.10.7: 'SRR11977931' is valid 2020-07-14T01:38:24 prefetch.2.10.7: 1) 'SRR11977931' was downloaded successfully 2020-07-14T01:38:24 prefetch.2.10.7: 'SRR11977931' has 0 unresolved dependencies Read 8645 spots for SRR11977931/SRR11977931.sra Written 8645 spots for SRR11977931/SRR11977931.sra 2020-07-14T01:38:34 prefetch.2.10.7: 1) Downloading 'SRR11977932'... 2020-07-14T01:38:34 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:39:54 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:39:55 prefetch.2.10.7: 'SRR11977932' is valid 2020-07-14T01:39:55 prefetch.2.10.7: 1) 'SRR11977932' was downloaded successfully 2020-07-14T01:39:55 prefetch.2.10.7: 'SRR11977932' has 0 unresolved dependencies Read 10110282 spots for SRR11977932/SRR11977932.sra Written 10110282 spots for SRR11977932/SRR11977932.sra 2020-07-14T01:40:45 prefetch.2.10.7: 1) Downloading 'SRR11977933'... 2020-07-14T01:40:45 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:42:50 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:42:50 prefetch.2.10.7: 'SRR11977933' is valid 2020-07-14T01:42:50 prefetch.2.10.7: 1) 'SRR11977933' was downloaded successfully 2020-07-14T01:42:50 prefetch.2.10.7: 'SRR11977933' has 0 unresolved dependencies Read 9804126 spots for SRR11977933/SRR11977933.sra Written 9804126 spots for SRR11977933/SRR11977933.sra 2020-07-14T01:43:36 prefetch.2.10.7: 1) Downloading 'SRR11977934'... 2020-07-14T01:43:36 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:45:10 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:45:11 prefetch.2.10.7: 'SRR11977934' is valid 2020-07-14T01:45:11 prefetch.2.10.7: 1) 'SRR11977934' was downloaded successfully 2020-07-14T01:45:11 prefetch.2.10.7: 'SRR11977934' has 0 unresolved dependencies Read 10078092 spots for SRR11977934/SRR11977934.sra Written 10078092 spots for SRR11977934/SRR11977934.sra 2020-07-14T01:46:01 prefetch.2.10.7: 1) Downloading 'SRR11977935'... 2020-07-14T01:46:01 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:47:10 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:47:11 prefetch.2.10.7: 'SRR11977935' is valid 2020-07-14T01:47:11 prefetch.2.10.7: 1) 'SRR11977935' was downloaded successfully 2020-07-14T01:47:11 prefetch.2.10.7: 'SRR11977935' has 0 unresolved dependencies Read 9795517 spots for SRR11977935/SRR11977935.sra Written 9795517 spots for SRR11977935/SRR11977935.sra 2020-07-14T01:47:57 prefetch.2.10.7: 1) Downloading 'SRR11977936'... 2020-07-14T01:47:57 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:48:09 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:48:09 prefetch.2.10.7: 'SRR11977936' is valid 2020-07-14T01:48:09 prefetch.2.10.7: 1) 'SRR11977936' was downloaded successfully 2020-07-14T01:48:09 prefetch.2.10.7: 'SRR11977936' has 0 unresolved dependencies Read 7147 spots for SRR11977936/SRR11977936.sra Written 7147 spots for SRR11977936/SRR11977936.sra 2020-07-14T01:48:19 prefetch.2.10.7: 1) Downloading 'SRR11978785'... 2020-07-14T01:48:19 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:48:38 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:48:38 prefetch.2.10.7: 'SRR11978785' is valid 2020-07-14T01:48:38 prefetch.2.10.7: 1) 'SRR11978785' was downloaded successfully 2020-07-14T01:48:38 prefetch.2.10.7: 'SRR11978785' has 0 unresolved dependencies Read 8636 spots for SRR11978785/SRR11978785.sra Written 8636 spots for SRR11978785/SRR11978785.sra 2020-07-14T01:48:53 prefetch.2.10.7: 1) Downloading 'SRR11978786'... 2020-07-14T01:48:53 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:49:01 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:49:01 prefetch.2.10.7: 'SRR11978786' is valid 2020-07-14T01:49:01 prefetch.2.10.7: 1) 'SRR11978786' was downloaded successfully 2020-07-14T01:49:01 prefetch.2.10.7: 'SRR11978786' has 0 unresolved dependencies Read 6618 spots for SRR11978786/SRR11978786.sra Written 6618 spots for SRR11978786/SRR11978786.sra 2020-07-14T01:49:12 prefetch.2.10.7: 1) Downloading 'SRR11978787'... 2020-07-14T01:49:12 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:49:21 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:49:21 prefetch.2.10.7: 'SRR11978787' is valid 2020-07-14T01:49:21 prefetch.2.10.7: 1) 'SRR11978787' was downloaded successfully 2020-07-14T01:49:21 prefetch.2.10.7: 'SRR11978787' has 0 unresolved dependencies Read 7101 spots for SRR11978787/SRR11978787.sra Written 7101 spots for SRR11978787/SRR11978787.sra 2020-07-14T01:49:31 prefetch.2.10.7: 1) Downloading 'SRR11978788'... 2020-07-14T01:49:31 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:49:40 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:49:40 prefetch.2.10.7: 'SRR11978788' is valid 2020-07-14T01:49:40 prefetch.2.10.7: 1) 'SRR11978788' was downloaded successfully 2020-07-14T01:49:40 prefetch.2.10.7: 'SRR11978788' has 0 unresolved dependencies Read 6985 spots for SRR11978788/SRR11978788.sra Written 6985 spots for SRR11978788/SRR11978788.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627469 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:06:33 43578863 reads; of these: 43578863 (100.00%) were unpaired; of these: 35731813 (81.99%) aligned 0 times 6198779 (14.22%) aligned exactly 1 time 1648271 (3.78%) aligned >1 times 18.01% overall alignment rate Time searching: 00:06:34 Overall time: 00:06:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1562189 / 7847050 = 0.1991 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:59:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:59:15: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:59:15: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:59:22: 1000000 INFO @ Tue, 14 Jul 2020 10:59:29: 2000000 INFO @ Tue, 14 Jul 2020 10:59:36: 3000000 INFO @ Tue, 14 Jul 2020 10:59:43: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:59:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:59:45: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:59:45: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:59:50: 5000000 INFO @ Tue, 14 Jul 2020 10:59:52: 1000000 INFO @ Tue, 14 Jul 2020 10:59:57: 6000000 INFO @ Tue, 14 Jul 2020 10:59:59: #1 tag size is determined as 54 bps INFO @ Tue, 14 Jul 2020 10:59:59: #1 tag size = 54 INFO @ Tue, 14 Jul 2020 10:59:59: #1 total tags in treatment: 6284861 INFO @ Tue, 14 Jul 2020 10:59:59: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:59:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:59:59: #1 tags after filtering in treatment: 6284861 INFO @ Tue, 14 Jul 2020 10:59:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:59:59: #1 finished! INFO @ Tue, 14 Jul 2020 10:59:59: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:59:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:59:59: 2000000 INFO @ Tue, 14 Jul 2020 11:00:00: #2 number of paired peaks: 296 WARNING @ Tue, 14 Jul 2020 11:00:00: Fewer paired peaks (296) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 296 pairs to build model! INFO @ Tue, 14 Jul 2020 11:00:00: start model_add_line... INFO @ Tue, 14 Jul 2020 11:00:00: start X-correlation... INFO @ Tue, 14 Jul 2020 11:00:00: end of X-cor INFO @ Tue, 14 Jul 2020 11:00:00: #2 finished! INFO @ Tue, 14 Jul 2020 11:00:00: #2 predicted fragment length is 46 bps INFO @ Tue, 14 Jul 2020 11:00:00: #2 alternative fragment length(s) may be 4,46,546,567 bps INFO @ Tue, 14 Jul 2020 11:00:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.05_model.r WARNING @ Tue, 14 Jul 2020 11:00:00: #2 Since the d (46) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:00:00: #2 You may need to consider one of the other alternative d(s): 4,46,546,567 WARNING @ Tue, 14 Jul 2020 11:00:00: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:00:00: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:00:00: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:00:06: 3000000 INFO @ Tue, 14 Jul 2020 11:00:13: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:00:13: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:00:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:00:15: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:00:15: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:00:20: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.05_peaks.xls INFO @ Tue, 14 Jul 2020 11:00:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:00:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.05_summits.bed INFO @ Tue, 14 Jul 2020 11:00:20: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1636 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:00:20: 5000000 INFO @ Tue, 14 Jul 2020 11:00:23: 1000000 INFO @ Tue, 14 Jul 2020 11:00:28: 6000000 INFO @ Tue, 14 Jul 2020 11:00:30: #1 tag size is determined as 54 bps INFO @ Tue, 14 Jul 2020 11:00:30: #1 tag size = 54 INFO @ Tue, 14 Jul 2020 11:00:30: #1 total tags in treatment: 6284861 INFO @ Tue, 14 Jul 2020 11:00:30: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:00:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:00:30: #1 tags after filtering in treatment: 6284861 INFO @ Tue, 14 Jul 2020 11:00:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:00:30: #1 finished! INFO @ Tue, 14 Jul 2020 11:00:30: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:00:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:00:30: #2 number of paired peaks: 296 WARNING @ Tue, 14 Jul 2020 11:00:30: Fewer paired peaks (296) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 296 pairs to build model! INFO @ Tue, 14 Jul 2020 11:00:30: start model_add_line... INFO @ Tue, 14 Jul 2020 11:00:30: start X-correlation... INFO @ Tue, 14 Jul 2020 11:00:30: end of X-cor INFO @ Tue, 14 Jul 2020 11:00:30: #2 finished! INFO @ Tue, 14 Jul 2020 11:00:30: #2 predicted fragment length is 46 bps INFO @ Tue, 14 Jul 2020 11:00:30: #2 alternative fragment length(s) may be 4,46,546,567 bps INFO @ Tue, 14 Jul 2020 11:00:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.10_model.r WARNING @ Tue, 14 Jul 2020 11:00:30: #2 Since the d (46) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:00:30: #2 You may need to consider one of the other alternative d(s): 4,46,546,567 WARNING @ Tue, 14 Jul 2020 11:00:30: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:00:30: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:00:30: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:00:30: 2000000 INFO @ Tue, 14 Jul 2020 11:00:37: 3000000 INFO @ Tue, 14 Jul 2020 11:00:43: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:00:44: 4000000 INFO @ Tue, 14 Jul 2020 11:00:50: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.10_peaks.xls INFO @ Tue, 14 Jul 2020 11:00:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:00:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.10_summits.bed INFO @ Tue, 14 Jul 2020 11:00:50: Done! pass1 - making usageList (11 chroms): 0 millis pass2 - checking and writing primary data (572 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 11:00:50: 5000000 INFO @ Tue, 14 Jul 2020 11:00:57: 6000000 INFO @ Tue, 14 Jul 2020 11:00:59: #1 tag size is determined as 54 bps INFO @ Tue, 14 Jul 2020 11:00:59: #1 tag size = 54 INFO @ Tue, 14 Jul 2020 11:00:59: #1 total tags in treatment: 6284861 INFO @ Tue, 14 Jul 2020 11:00:59: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:00:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:00:59: #1 tags after filtering in treatment: 6284861 INFO @ Tue, 14 Jul 2020 11:00:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:00:59: #1 finished! INFO @ Tue, 14 Jul 2020 11:00:59: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:00:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:01:00: #2 number of paired peaks: 296 WARNING @ Tue, 14 Jul 2020 11:01:00: Fewer paired peaks (296) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 296 pairs to build model! INFO @ Tue, 14 Jul 2020 11:01:00: start model_add_line... INFO @ Tue, 14 Jul 2020 11:01:00: start X-correlation... INFO @ Tue, 14 Jul 2020 11:01:00: end of X-cor INFO @ Tue, 14 Jul 2020 11:01:00: #2 finished! INFO @ Tue, 14 Jul 2020 11:01:00: #2 predicted fragment length is 46 bps INFO @ Tue, 14 Jul 2020 11:01:00: #2 alternative fragment length(s) may be 4,46,546,567 bps INFO @ Tue, 14 Jul 2020 11:01:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.20_model.r WARNING @ Tue, 14 Jul 2020 11:01:00: #2 Since the d (46) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:01:00: #2 You may need to consider one of the other alternative d(s): 4,46,546,567 WARNING @ Tue, 14 Jul 2020 11:01:00: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:01:00: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:01:00: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 11:01:14: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:01:21: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.20_peaks.xls INFO @ Tue, 14 Jul 2020 11:01:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:01:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521319/SRX8521319.20_summits.bed INFO @ Tue, 14 Jul 2020 11:01:22: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (74 records, 4 fields): 1 millis CompletedMACS2peakCalling