Job ID = 6627233 SRX = SRX8521318 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T01:33:36 prefetch.2.10.7: 1) Downloading 'SRR11978769'... 2020-07-14T01:33:36 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:33:50 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:33:50 prefetch.2.10.7: 'SRR11978769' is valid 2020-07-14T01:33:50 prefetch.2.10.7: 1) 'SRR11978769' was downloaded successfully 2020-07-14T01:33:50 prefetch.2.10.7: 'SRR11978769' has 0 unresolved dependencies Read 31970 spots for SRR11978769/SRR11978769.sra Written 31970 spots for SRR11978769/SRR11978769.sra 2020-07-14T01:34:01 prefetch.2.10.7: 1) Downloading 'SRR11978770'... 2020-07-14T01:34:01 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:34:16 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:34:16 prefetch.2.10.7: 'SRR11978770' is valid 2020-07-14T01:34:16 prefetch.2.10.7: 1) 'SRR11978770' was downloaded successfully 2020-07-14T01:34:16 prefetch.2.10.7: 'SRR11978770' has 0 unresolved dependencies Read 113853 spots for SRR11978770/SRR11978770.sra Written 113853 spots for SRR11978770/SRR11978770.sra 2020-07-14T01:34:28 prefetch.2.10.7: 1) Downloading 'SRR11978771'... 2020-07-14T01:34:28 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:34:40 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:34:40 prefetch.2.10.7: 'SRR11978771' is valid 2020-07-14T01:34:40 prefetch.2.10.7: 1) 'SRR11978771' was downloaded successfully 2020-07-14T01:34:40 prefetch.2.10.7: 'SRR11978771' has 0 unresolved dependencies Read 122637 spots for SRR11978771/SRR11978771.sra Written 122637 spots for SRR11978771/SRR11978771.sra 2020-07-14T01:34:54 prefetch.2.10.7: 1) Downloading 'SRR11978772'... 2020-07-14T01:34:54 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:35:11 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:35:11 prefetch.2.10.7: 'SRR11978772' is valid 2020-07-14T01:35:11 prefetch.2.10.7: 1) 'SRR11978772' was downloaded successfully 2020-07-14T01:35:11 prefetch.2.10.7: 'SRR11978772' has 0 unresolved dependencies Read 121083 spots for SRR11978772/SRR11978772.sra Written 121083 spots for SRR11978772/SRR11978772.sra 2020-07-14T01:35:26 prefetch.2.10.7: 1) Downloading 'SRR11978773'... 2020-07-14T01:35:26 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:35:44 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:35:45 prefetch.2.10.7: 'SRR11978773' is valid 2020-07-14T01:35:45 prefetch.2.10.7: 1) 'SRR11978773' was downloaded successfully 2020-07-14T01:35:45 prefetch.2.10.7: 'SRR11978773' has 0 unresolved dependencies Read 646691 spots for SRR11978773/SRR11978773.sra Written 646691 spots for SRR11978773/SRR11978773.sra 2020-07-14T01:35:57 prefetch.2.10.7: 1) Downloading 'SRR11978774'... 2020-07-14T01:35:57 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:36:22 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:36:22 prefetch.2.10.7: 'SRR11978774' is valid 2020-07-14T01:36:22 prefetch.2.10.7: 1) 'SRR11978774' was downloaded successfully 2020-07-14T01:36:22 prefetch.2.10.7: 'SRR11978774' has 0 unresolved dependencies Read 643066 spots for SRR11978774/SRR11978774.sra Written 643066 spots for SRR11978774/SRR11978774.sra 2020-07-14T01:36:39 prefetch.2.10.7: 1) Downloading 'SRR11978775'... 2020-07-14T01:36:39 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:36:55 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:36:55 prefetch.2.10.7: 'SRR11978775' is valid 2020-07-14T01:36:55 prefetch.2.10.7: 1) 'SRR11978775' was downloaded successfully 2020-07-14T01:36:55 prefetch.2.10.7: 'SRR11978775' has 0 unresolved dependencies Read 651979 spots for SRR11978775/SRR11978775.sra Written 651979 spots for SRR11978775/SRR11978775.sra 2020-07-14T01:37:08 prefetch.2.10.7: 1) Downloading 'SRR11978776'... 2020-07-14T01:37:08 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:37:29 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:37:29 prefetch.2.10.7: 'SRR11978776' is valid 2020-07-14T01:37:29 prefetch.2.10.7: 1) 'SRR11978776' was downloaded successfully 2020-07-14T01:37:29 prefetch.2.10.7: 'SRR11978776' has 0 unresolved dependencies Read 636989 spots for SRR11978776/SRR11978776.sra Written 636989 spots for SRR11978776/SRR11978776.sra 2020-07-14T01:37:48 prefetch.2.10.7: 1) Downloading 'SRR11978777'... 2020-07-14T01:37:48 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:37:58 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:37:58 prefetch.2.10.7: 'SRR11978777' is valid 2020-07-14T01:37:58 prefetch.2.10.7: 1) 'SRR11978777' was downloaded successfully 2020-07-14T01:37:58 prefetch.2.10.7: 'SRR11978777' has 0 unresolved dependencies Read 30196 spots for SRR11978777/SRR11978777.sra Written 30196 spots for SRR11978777/SRR11978777.sra 2020-07-14T01:38:09 prefetch.2.10.7: 1) Downloading 'SRR11978778'... 2020-07-14T01:38:09 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:38:22 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:38:22 prefetch.2.10.7: 'SRR11978778' is valid 2020-07-14T01:38:22 prefetch.2.10.7: 1) 'SRR11978778' was downloaded successfully 2020-07-14T01:38:22 prefetch.2.10.7: 'SRR11978778' has 0 unresolved dependencies Read 31698 spots for SRR11978778/SRR11978778.sra Written 31698 spots for SRR11978778/SRR11978778.sra 2020-07-14T01:38:44 prefetch.2.10.7: 1) Downloading 'SRR11978779'... 2020-07-14T01:38:44 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:38:54 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:38:54 prefetch.2.10.7: 'SRR11978779' is valid 2020-07-14T01:38:54 prefetch.2.10.7: 1) 'SRR11978779' was downloaded successfully 2020-07-14T01:38:54 prefetch.2.10.7: 'SRR11978779' has 0 unresolved dependencies Read 31389 spots for SRR11978779/SRR11978779.sra Written 31389 spots for SRR11978779/SRR11978779.sra 2020-07-14T01:39:08 prefetch.2.10.7: 1) Downloading 'SRR11978780'... 2020-07-14T01:39:08 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:40:17 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:40:18 prefetch.2.10.7: 'SRR11978780' is valid 2020-07-14T01:40:18 prefetch.2.10.7: 1) 'SRR11978780' was downloaded successfully 2020-07-14T01:40:18 prefetch.2.10.7: 'SRR11978780' has 0 unresolved dependencies Read 9506744 spots for SRR11978780/SRR11978780.sra Written 9506744 spots for SRR11978780/SRR11978780.sra 2020-07-14T01:41:01 prefetch.2.10.7: 1) Downloading 'SRR11978781'... 2020-07-14T01:41:01 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:42:08 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:42:08 prefetch.2.10.7: 'SRR11978781' is valid 2020-07-14T01:42:08 prefetch.2.10.7: 1) 'SRR11978781' was downloaded successfully 2020-07-14T01:42:08 prefetch.2.10.7: 'SRR11978781' has 0 unresolved dependencies Read 9141859 spots for SRR11978781/SRR11978781.sra Written 9141859 spots for SRR11978781/SRR11978781.sra 2020-07-14T01:42:49 prefetch.2.10.7: 1) Downloading 'SRR11978782'... 2020-07-14T01:42:49 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:45:18 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:45:18 prefetch.2.10.7: 'SRR11978782' is valid 2020-07-14T01:45:18 prefetch.2.10.7: 1) 'SRR11978782' was downloaded successfully 2020-07-14T01:45:18 prefetch.2.10.7: 'SRR11978782' has 0 unresolved dependencies Read 9444908 spots for SRR11978782/SRR11978782.sra Written 9444908 spots for SRR11978782/SRR11978782.sra 2020-07-14T01:46:13 prefetch.2.10.7: 1) Downloading 'SRR11978783'... 2020-07-14T01:46:13 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:47:53 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:47:53 prefetch.2.10.7: 'SRR11978783' is valid 2020-07-14T01:47:53 prefetch.2.10.7: 1) 'SRR11978783' was downloaded successfully 2020-07-14T01:47:53 prefetch.2.10.7: 'SRR11978783' has 0 unresolved dependencies Read 9203969 spots for SRR11978783/SRR11978783.sra Written 9203969 spots for SRR11978783/SRR11978783.sra 2020-07-14T01:48:40 prefetch.2.10.7: 1) Downloading 'SRR11978784'... 2020-07-14T01:48:40 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:49:04 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:49:04 prefetch.2.10.7: 'SRR11978784' is valid 2020-07-14T01:49:04 prefetch.2.10.7: 1) 'SRR11978784' was downloaded successfully 2020-07-14T01:49:04 prefetch.2.10.7: 'SRR11978784' has 0 unresolved dependencies Read 122171 spots for SRR11978784/SRR11978784.sra Written 122171 spots for SRR11978784/SRR11978784.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627465 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:03 40481202 reads; of these: 40481202 (100.00%) were unpaired; of these: 34448971 (85.10%) aligned 0 times 4747380 (11.73%) aligned exactly 1 time 1284851 (3.17%) aligned >1 times 14.90% overall alignment rate Time searching: 00:06:03 Overall time: 00:06:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1415131 / 6032231 = 0.2346 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:57:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:57:37: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:57:37: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:57:44: 1000000 INFO @ Tue, 14 Jul 2020 10:57:50: 2000000 INFO @ Tue, 14 Jul 2020 10:57:55: 3000000 INFO @ Tue, 14 Jul 2020 10:58:01: 4000000 INFO @ Tue, 14 Jul 2020 10:58:05: #1 tag size is determined as 64 bps INFO @ Tue, 14 Jul 2020 10:58:05: #1 tag size = 64 INFO @ Tue, 14 Jul 2020 10:58:05: #1 total tags in treatment: 4617100 INFO @ Tue, 14 Jul 2020 10:58:05: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:58:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:58:05: #1 tags after filtering in treatment: 4617100 INFO @ Tue, 14 Jul 2020 10:58:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:58:05: #1 finished! INFO @ Tue, 14 Jul 2020 10:58:05: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:58:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:58:05: #2 number of paired peaks: 404 WARNING @ Tue, 14 Jul 2020 10:58:05: Fewer paired peaks (404) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 404 pairs to build model! INFO @ Tue, 14 Jul 2020 10:58:05: start model_add_line... INFO @ Tue, 14 Jul 2020 10:58:05: start X-correlation... INFO @ Tue, 14 Jul 2020 10:58:05: end of X-cor INFO @ Tue, 14 Jul 2020 10:58:05: #2 finished! INFO @ Tue, 14 Jul 2020 10:58:05: #2 predicted fragment length is 52 bps INFO @ Tue, 14 Jul 2020 10:58:05: #2 alternative fragment length(s) may be 52,530,549 bps INFO @ Tue, 14 Jul 2020 10:58:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.05_model.r WARNING @ Tue, 14 Jul 2020 10:58:05: #2 Since the d (52) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 10:58:05: #2 You may need to consider one of the other alternative d(s): 52,530,549 WARNING @ Tue, 14 Jul 2020 10:58:05: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 10:58:05: #3 Call peaks... INFO @ Tue, 14 Jul 2020 10:58:05: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:58:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:58:07: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:58:07: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:58:14: 1000000 INFO @ Tue, 14 Jul 2020 10:58:15: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 10:58:20: 2000000 INFO @ Tue, 14 Jul 2020 10:58:21: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.05_peaks.xls INFO @ Tue, 14 Jul 2020 10:58:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 10:58:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.05_summits.bed INFO @ Tue, 14 Jul 2020 10:58:21: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1189 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:58:26: 3000000 INFO @ Tue, 14 Jul 2020 10:58:33: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:58:36: #1 tag size is determined as 64 bps INFO @ Tue, 14 Jul 2020 10:58:36: #1 tag size = 64 INFO @ Tue, 14 Jul 2020 10:58:36: #1 total tags in treatment: 4617100 INFO @ Tue, 14 Jul 2020 10:58:36: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:58:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:58:36: #1 tags after filtering in treatment: 4617100 INFO @ Tue, 14 Jul 2020 10:58:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:58:36: #1 finished! INFO @ Tue, 14 Jul 2020 10:58:36: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:58:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:58:36: #2 number of paired peaks: 404 WARNING @ Tue, 14 Jul 2020 10:58:36: Fewer paired peaks (404) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 404 pairs to build model! INFO @ Tue, 14 Jul 2020 10:58:36: start model_add_line... INFO @ Tue, 14 Jul 2020 10:58:36: start X-correlation... INFO @ Tue, 14 Jul 2020 10:58:36: end of X-cor INFO @ Tue, 14 Jul 2020 10:58:36: #2 finished! INFO @ Tue, 14 Jul 2020 10:58:36: #2 predicted fragment length is 52 bps INFO @ Tue, 14 Jul 2020 10:58:36: #2 alternative fragment length(s) may be 52,530,549 bps INFO @ Tue, 14 Jul 2020 10:58:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.10_model.r WARNING @ Tue, 14 Jul 2020 10:58:36: #2 Since the d (52) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 10:58:36: #2 You may need to consider one of the other alternative d(s): 52,530,549 WARNING @ Tue, 14 Jul 2020 10:58:36: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 10:58:36: #3 Call peaks... INFO @ Tue, 14 Jul 2020 10:58:36: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 10:58:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:58:37: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:58:37: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:58:43: 1000000 INFO @ Tue, 14 Jul 2020 10:58:47: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 10:58:50: 2000000 INFO @ Tue, 14 Jul 2020 10:58:52: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.10_peaks.xls INFO @ Tue, 14 Jul 2020 10:58:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 10:58:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.10_summits.bed INFO @ Tue, 14 Jul 2020 10:58:52: Done! pass1 - making usageList (11 chroms): 1 millis pass2 - checking and writing primary data (436 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:58:56: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 10:59:02: 4000000 INFO @ Tue, 14 Jul 2020 10:59:06: #1 tag size is determined as 64 bps INFO @ Tue, 14 Jul 2020 10:59:06: #1 tag size = 64 INFO @ Tue, 14 Jul 2020 10:59:06: #1 total tags in treatment: 4617100 INFO @ Tue, 14 Jul 2020 10:59:06: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:59:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:59:06: #1 tags after filtering in treatment: 4617100 INFO @ Tue, 14 Jul 2020 10:59:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:59:06: #1 finished! INFO @ Tue, 14 Jul 2020 10:59:06: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:59:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:59:06: #2 number of paired peaks: 404 WARNING @ Tue, 14 Jul 2020 10:59:06: Fewer paired peaks (404) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 404 pairs to build model! INFO @ Tue, 14 Jul 2020 10:59:06: start model_add_line... INFO @ Tue, 14 Jul 2020 10:59:06: start X-correlation... INFO @ Tue, 14 Jul 2020 10:59:06: end of X-cor INFO @ Tue, 14 Jul 2020 10:59:06: #2 finished! INFO @ Tue, 14 Jul 2020 10:59:06: #2 predicted fragment length is 52 bps INFO @ Tue, 14 Jul 2020 10:59:06: #2 alternative fragment length(s) may be 52,530,549 bps INFO @ Tue, 14 Jul 2020 10:59:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.20_model.r WARNING @ Tue, 14 Jul 2020 10:59:06: #2 Since the d (52) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 10:59:06: #2 You may need to consider one of the other alternative d(s): 52,530,549 WARNING @ Tue, 14 Jul 2020 10:59:06: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 10:59:06: #3 Call peaks... INFO @ Tue, 14 Jul 2020 10:59:06: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 10:59:16: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 10:59:21: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.20_peaks.xls INFO @ Tue, 14 Jul 2020 10:59:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 10:59:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521318/SRX8521318.20_summits.bed INFO @ Tue, 14 Jul 2020 10:59:21: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (99 records, 4 fields): 1 millis CompletedMACS2peakCalling