Job ID = 6627199 SRX = SRX8521317 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T01:33:05 prefetch.2.10.7: 1) Downloading 'SRR11978765'... 2020-07-14T01:33:05 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:37:33 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:37:33 prefetch.2.10.7: 1) 'SRR11978765' was downloaded successfully 2020-07-14T01:37:33 prefetch.2.10.7: 'SRR11978765' has 0 unresolved dependencies Read 39374128 spots for SRR11978765/SRR11978765.sra Written 39374128 spots for SRR11978765/SRR11978765.sra 2020-07-14T01:40:04 prefetch.2.10.7: 1) Downloading 'SRR11978766'... 2020-07-14T01:40:04 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:45:19 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:45:19 prefetch.2.10.7: 1) 'SRR11978766' was downloaded successfully 2020-07-14T01:45:19 prefetch.2.10.7: 'SRR11978766' has 0 unresolved dependencies Read 37542895 spots for SRR11978766/SRR11978766.sra Written 37542895 spots for SRR11978766/SRR11978766.sra 2020-07-14T01:47:37 prefetch.2.10.7: 1) Downloading 'SRR11978767'... 2020-07-14T01:47:37 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:52:53 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:52:53 prefetch.2.10.7: 1) 'SRR11978767' was downloaded successfully 2020-07-14T01:52:53 prefetch.2.10.7: 'SRR11978767' has 0 unresolved dependencies Read 38860110 spots for SRR11978767/SRR11978767.sra Written 38860110 spots for SRR11978767/SRR11978767.sra 2020-07-14T01:55:12 prefetch.2.10.7: 1) Downloading 'SRR11978768'... 2020-07-14T01:55:12 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T02:00:06 prefetch.2.10.7: HTTPS download succeed 2020-07-14T02:00:06 prefetch.2.10.7: 1) 'SRR11978768' was downloaded successfully 2020-07-14T02:00:06 prefetch.2.10.7: 'SRR11978768' has 0 unresolved dependencies Read 38327392 spots for SRR11978768/SRR11978768.sra Written 38327392 spots for SRR11978768/SRR11978768.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627612 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:37:28 154104525 reads; of these: 154104525 (100.00%) were unpaired; of these: 114881761 (74.55%) aligned 0 times 24139623 (15.66%) aligned exactly 1 time 15083141 (9.79%) aligned >1 times 25.45% overall alignment rate Time searching: 00:37:29 Overall time: 00:37:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 15401441 / 39222764 = 0.3927 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:50:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:50:46: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:50:46: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:50:52: 1000000 INFO @ Tue, 14 Jul 2020 11:50:58: 2000000 INFO @ Tue, 14 Jul 2020 11:51:04: 3000000 INFO @ Tue, 14 Jul 2020 11:51:10: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:51:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:51:16: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:51:16: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:51:16: 5000000 INFO @ Tue, 14 Jul 2020 11:51:21: 1000000 INFO @ Tue, 14 Jul 2020 11:51:22: 6000000 INFO @ Tue, 14 Jul 2020 11:51:27: 2000000 INFO @ Tue, 14 Jul 2020 11:51:29: 7000000 INFO @ Tue, 14 Jul 2020 11:51:32: 3000000 INFO @ Tue, 14 Jul 2020 11:51:35: 8000000 INFO @ Tue, 14 Jul 2020 11:51:38: 4000000 INFO @ Tue, 14 Jul 2020 11:51:41: 9000000 INFO @ Tue, 14 Jul 2020 11:51:43: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:51:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:51:46: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:51:46: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:51:47: 10000000 INFO @ Tue, 14 Jul 2020 11:51:49: 6000000 INFO @ Tue, 14 Jul 2020 11:51:52: 1000000 INFO @ Tue, 14 Jul 2020 11:51:54: 11000000 INFO @ Tue, 14 Jul 2020 11:51:54: 7000000 INFO @ Tue, 14 Jul 2020 11:51:59: 2000000 INFO @ Tue, 14 Jul 2020 11:52:00: 12000000 INFO @ Tue, 14 Jul 2020 11:52:00: 8000000 INFO @ Tue, 14 Jul 2020 11:52:05: 3000000 INFO @ Tue, 14 Jul 2020 11:52:06: 9000000 INFO @ Tue, 14 Jul 2020 11:52:06: 13000000 INFO @ Tue, 14 Jul 2020 11:52:12: 10000000 INFO @ Tue, 14 Jul 2020 11:52:12: 4000000 INFO @ Tue, 14 Jul 2020 11:52:13: 14000000 INFO @ Tue, 14 Jul 2020 11:52:17: 11000000 INFO @ Tue, 14 Jul 2020 11:52:18: 5000000 INFO @ Tue, 14 Jul 2020 11:52:19: 15000000 INFO @ Tue, 14 Jul 2020 11:52:22: 12000000 INFO @ Tue, 14 Jul 2020 11:52:24: 6000000 INFO @ Tue, 14 Jul 2020 11:52:24: 16000000 INFO @ Tue, 14 Jul 2020 11:52:28: 13000000 INFO @ Tue, 14 Jul 2020 11:52:30: 17000000 INFO @ Tue, 14 Jul 2020 11:52:30: 7000000 INFO @ Tue, 14 Jul 2020 11:52:33: 14000000 INFO @ Tue, 14 Jul 2020 11:52:37: 18000000 INFO @ Tue, 14 Jul 2020 11:52:37: 8000000 INFO @ Tue, 14 Jul 2020 11:52:39: 15000000 INFO @ Tue, 14 Jul 2020 11:52:43: 19000000 INFO @ Tue, 14 Jul 2020 11:52:43: 9000000 INFO @ Tue, 14 Jul 2020 11:52:44: 16000000 INFO @ Tue, 14 Jul 2020 11:52:49: 20000000 INFO @ Tue, 14 Jul 2020 11:52:49: 17000000 INFO @ Tue, 14 Jul 2020 11:52:50: 10000000 INFO @ Tue, 14 Jul 2020 11:52:55: 18000000 INFO @ Tue, 14 Jul 2020 11:52:55: 21000000 INFO @ Tue, 14 Jul 2020 11:52:56: 11000000 INFO @ Tue, 14 Jul 2020 11:53:00: 19000000 INFO @ Tue, 14 Jul 2020 11:53:01: 22000000 INFO @ Tue, 14 Jul 2020 11:53:03: 12000000 INFO @ Tue, 14 Jul 2020 11:53:05: 20000000 INFO @ Tue, 14 Jul 2020 11:53:08: 23000000 INFO @ Tue, 14 Jul 2020 11:53:09: 13000000 INFO @ Tue, 14 Jul 2020 11:53:11: 21000000 INFO @ Tue, 14 Jul 2020 11:53:13: #1 tag size is determined as 65 bps INFO @ Tue, 14 Jul 2020 11:53:13: #1 tag size = 65 INFO @ Tue, 14 Jul 2020 11:53:13: #1 total tags in treatment: 23821323 INFO @ Tue, 14 Jul 2020 11:53:13: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:53:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:53:13: #1 tags after filtering in treatment: 23821323 INFO @ Tue, 14 Jul 2020 11:53:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:53:13: #1 finished! INFO @ Tue, 14 Jul 2020 11:53:13: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:53:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:53:15: #2 number of paired peaks: 7 WARNING @ Tue, 14 Jul 2020 11:53:15: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 11:53:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:53:15: 14000000 INFO @ Tue, 14 Jul 2020 11:53:16: 22000000 INFO @ Tue, 14 Jul 2020 11:53:21: 15000000 INFO @ Tue, 14 Jul 2020 11:53:21: 23000000 INFO @ Tue, 14 Jul 2020 11:53:26: #1 tag size is determined as 65 bps INFO @ Tue, 14 Jul 2020 11:53:26: #1 tag size = 65 INFO @ Tue, 14 Jul 2020 11:53:26: #1 total tags in treatment: 23821323 INFO @ Tue, 14 Jul 2020 11:53:26: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:53:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:53:26: #1 tags after filtering in treatment: 23821323 INFO @ Tue, 14 Jul 2020 11:53:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:53:26: #1 finished! INFO @ Tue, 14 Jul 2020 11:53:26: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:53:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:53:27: 16000000 INFO @ Tue, 14 Jul 2020 11:53:28: #2 number of paired peaks: 7 WARNING @ Tue, 14 Jul 2020 11:53:28: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 11:53:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:53:33: 17000000 INFO @ Tue, 14 Jul 2020 11:53:39: 18000000 INFO @ Tue, 14 Jul 2020 11:53:45: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 11:53:51: 20000000 INFO @ Tue, 14 Jul 2020 11:53:56: 21000000 INFO @ Tue, 14 Jul 2020 11:54:02: 22000000 INFO @ Tue, 14 Jul 2020 11:54:08: 23000000 INFO @ Tue, 14 Jul 2020 11:54:13: #1 tag size is determined as 65 bps INFO @ Tue, 14 Jul 2020 11:54:13: #1 tag size = 65 INFO @ Tue, 14 Jul 2020 11:54:13: #1 total tags in treatment: 23821323 INFO @ Tue, 14 Jul 2020 11:54:13: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:54:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:54:14: #1 tags after filtering in treatment: 23821323 INFO @ Tue, 14 Jul 2020 11:54:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:54:14: #1 finished! INFO @ Tue, 14 Jul 2020 11:54:14: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:54:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:54:15: #2 number of paired peaks: 7 WARNING @ Tue, 14 Jul 2020 11:54:15: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 11:54:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8521317/SRX8521317.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。