Job ID = 6627186 SRX = SRX8521313 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T01:29:31 prefetch.2.10.7: 1) Downloading 'SRR11978709'... 2020-07-14T01:29:31 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:29:50 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:29:50 prefetch.2.10.7: 'SRR11978709' is valid 2020-07-14T01:29:50 prefetch.2.10.7: 1) 'SRR11978709' was downloaded successfully 2020-07-14T01:29:50 prefetch.2.10.7: 'SRR11978709' has 0 unresolved dependencies Read 776973 spots for SRR11978709/SRR11978709.sra Written 776973 spots for SRR11978709/SRR11978709.sra 2020-07-14T01:30:04 prefetch.2.10.7: 1) Downloading 'SRR11978710'... 2020-07-14T01:30:04 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:30:20 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:30:20 prefetch.2.10.7: 'SRR11978710' is valid 2020-07-14T01:30:20 prefetch.2.10.7: 1) 'SRR11978710' was downloaded successfully 2020-07-14T01:30:20 prefetch.2.10.7: 'SRR11978710' has 0 unresolved dependencies Read 85639 spots for SRR11978710/SRR11978710.sra Written 85639 spots for SRR11978710/SRR11978710.sra 2020-07-14T01:30:31 prefetch.2.10.7: 1) Downloading 'SRR11978711'... 2020-07-14T01:30:31 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:30:47 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:30:47 prefetch.2.10.7: 'SRR11978711' is valid 2020-07-14T01:30:47 prefetch.2.10.7: 1) 'SRR11978711' was downloaded successfully 2020-07-14T01:30:47 prefetch.2.10.7: 'SRR11978711' has 0 unresolved dependencies Read 90787 spots for SRR11978711/SRR11978711.sra Written 90787 spots for SRR11978711/SRR11978711.sra 2020-07-14T01:30:58 prefetch.2.10.7: 1) Downloading 'SRR11978712'... 2020-07-14T01:30:58 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:31:13 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:31:13 prefetch.2.10.7: 'SRR11978712' is valid 2020-07-14T01:31:13 prefetch.2.10.7: 1) 'SRR11978712' was downloaded successfully 2020-07-14T01:31:13 prefetch.2.10.7: 'SRR11978712' has 0 unresolved dependencies Read 89424 spots for SRR11978712/SRR11978712.sra Written 89424 spots for SRR11978712/SRR11978712.sra 2020-07-14T01:31:27 prefetch.2.10.7: 1) Downloading 'SRR11978713'... 2020-07-14T01:31:27 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:32:24 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:32:25 prefetch.2.10.7: 'SRR11978713' is valid 2020-07-14T01:32:25 prefetch.2.10.7: 1) 'SRR11978713' was downloaded successfully 2020-07-14T01:32:25 prefetch.2.10.7: 'SRR11978713' has 0 unresolved dependencies Read 4464592 spots for SRR11978713/SRR11978713.sra Written 4464592 spots for SRR11978713/SRR11978713.sra 2020-07-14T01:32:50 prefetch.2.10.7: 1) Downloading 'SRR11978714'... 2020-07-14T01:32:50 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:33:38 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:33:38 prefetch.2.10.7: 'SRR11978714' is valid 2020-07-14T01:33:38 prefetch.2.10.7: 1) 'SRR11978714' was downloaded successfully 2020-07-14T01:33:38 prefetch.2.10.7: 'SRR11978714' has 0 unresolved dependencies Read 4492263 spots for SRR11978714/SRR11978714.sra Written 4492263 spots for SRR11978714/SRR11978714.sra 2020-07-14T01:34:05 prefetch.2.10.7: 1) Downloading 'SRR11978715'... 2020-07-14T01:34:05 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:34:41 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:34:41 prefetch.2.10.7: 'SRR11978715' is valid 2020-07-14T01:34:41 prefetch.2.10.7: 1) 'SRR11978715' was downloaded successfully 2020-07-14T01:34:41 prefetch.2.10.7: 'SRR11978715' has 0 unresolved dependencies Read 4507903 spots for SRR11978715/SRR11978715.sra Written 4507903 spots for SRR11978715/SRR11978715.sra 2020-07-14T01:35:10 prefetch.2.10.7: 1) Downloading 'SRR11978716'... 2020-07-14T01:35:10 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:35:47 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:35:48 prefetch.2.10.7: 'SRR11978716' is valid 2020-07-14T01:35:48 prefetch.2.10.7: 1) 'SRR11978716' was downloaded successfully 2020-07-14T01:35:48 prefetch.2.10.7: 'SRR11978716' has 0 unresolved dependencies Read 4483048 spots for SRR11978716/SRR11978716.sra Written 4483048 spots for SRR11978716/SRR11978716.sra 2020-07-14T01:36:17 prefetch.2.10.7: 1) Downloading 'SRR11978717'... 2020-07-14T01:36:17 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:36:43 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:36:43 prefetch.2.10.7: 'SRR11978717' is valid 2020-07-14T01:36:43 prefetch.2.10.7: 1) 'SRR11978717' was downloaded successfully 2020-07-14T01:36:43 prefetch.2.10.7: 'SRR11978717' has 0 unresolved dependencies Read 743397 spots for SRR11978717/SRR11978717.sra Written 743397 spots for SRR11978717/SRR11978717.sra 2020-07-14T01:36:56 prefetch.2.10.7: 1) Downloading 'SRR11978718'... 2020-07-14T01:36:56 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:37:12 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:37:12 prefetch.2.10.7: 'SRR11978718' is valid 2020-07-14T01:37:12 prefetch.2.10.7: 1) 'SRR11978718' was downloaded successfully 2020-07-14T01:37:12 prefetch.2.10.7: 'SRR11978718' has 0 unresolved dependencies Read 782191 spots for SRR11978718/SRR11978718.sra Written 782191 spots for SRR11978718/SRR11978718.sra 2020-07-14T01:37:29 prefetch.2.10.7: 1) Downloading 'SRR11978719'... 2020-07-14T01:37:29 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:37:49 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:37:49 prefetch.2.10.7: 'SRR11978719' is valid 2020-07-14T01:37:49 prefetch.2.10.7: 1) 'SRR11978719' was downloaded successfully 2020-07-14T01:37:49 prefetch.2.10.7: 'SRR11978719' has 0 unresolved dependencies Read 778345 spots for SRR11978719/SRR11978719.sra Written 778345 spots for SRR11978719/SRR11978719.sra 2020-07-14T01:38:02 prefetch.2.10.7: 1) Downloading 'SRR11978720'... 2020-07-14T01:38:02 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:39:04 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:39:05 prefetch.2.10.7: 'SRR11978720' is valid 2020-07-14T01:39:05 prefetch.2.10.7: 1) 'SRR11978720' was downloaded successfully 2020-07-14T01:39:05 prefetch.2.10.7: 'SRR11978720' has 0 unresolved dependencies Read 8856232 spots for SRR11978720/SRR11978720.sra Written 8856232 spots for SRR11978720/SRR11978720.sra 2020-07-14T01:39:45 prefetch.2.10.7: 1) Downloading 'SRR11978721'... 2020-07-14T01:39:45 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:41:22 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:41:22 prefetch.2.10.7: 'SRR11978721' is valid 2020-07-14T01:41:22 prefetch.2.10.7: 1) 'SRR11978721' was downloaded successfully 2020-07-14T01:41:22 prefetch.2.10.7: 'SRR11978721' has 0 unresolved dependencies Read 9009811 spots for SRR11978721/SRR11978721.sra Written 9009811 spots for SRR11978721/SRR11978721.sra 2020-07-14T01:42:03 prefetch.2.10.7: 1) Downloading 'SRR11978722'... 2020-07-14T01:42:03 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:43:59 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:43:59 prefetch.2.10.7: 'SRR11978722' is valid 2020-07-14T01:43:59 prefetch.2.10.7: 1) 'SRR11978722' was downloaded successfully 2020-07-14T01:43:59 prefetch.2.10.7: 'SRR11978722' has 0 unresolved dependencies Read 9053080 spots for SRR11978722/SRR11978722.sra Written 9053080 spots for SRR11978722/SRR11978722.sra 2020-07-14T01:44:44 prefetch.2.10.7: 1) Downloading 'SRR11978723'... 2020-07-14T01:44:44 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:47:39 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:47:40 prefetch.2.10.7: 'SRR11978723' is valid 2020-07-14T01:47:40 prefetch.2.10.7: 1) 'SRR11978723' was downloaded successfully 2020-07-14T01:47:40 prefetch.2.10.7: 'SRR11978723' has 0 unresolved dependencies Read 8858319 spots for SRR11978723/SRR11978723.sra Written 8858319 spots for SRR11978723/SRR11978723.sra 2020-07-14T01:48:19 prefetch.2.10.7: 1) Downloading 'SRR11978724'... 2020-07-14T01:48:19 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:48:41 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:48:41 prefetch.2.10.7: 'SRR11978724' is valid 2020-07-14T01:48:41 prefetch.2.10.7: 1) 'SRR11978724' was downloaded successfully 2020-07-14T01:48:41 prefetch.2.10.7: 'SRR11978724' has 0 unresolved dependencies Read 89027 spots for SRR11978724/SRR11978724.sra Written 89027 spots for SRR11978724/SRR11978724.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627475 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:46 57161031 reads; of these: 57161031 (100.00%) were unpaired; of these: 48246301 (84.40%) aligned 0 times 6888766 (12.05%) aligned exactly 1 time 2025964 (3.54%) aligned >1 times 15.60% overall alignment rate Time searching: 00:08:47 Overall time: 00:08:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1945505 / 8914730 = 0.2182 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:00:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:00:38: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:00:38: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:00:44: 1000000 INFO @ Tue, 14 Jul 2020 11:00:50: 2000000 INFO @ Tue, 14 Jul 2020 11:00:56: 3000000 INFO @ Tue, 14 Jul 2020 11:01:02: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:01:07: 5000000 INFO @ Tue, 14 Jul 2020 11:01:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:01:08: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:01:08: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:01:13: 6000000 INFO @ Tue, 14 Jul 2020 11:01:15: 1000000 INFO @ Tue, 14 Jul 2020 11:01:20: #1 tag size is determined as 62 bps INFO @ Tue, 14 Jul 2020 11:01:20: #1 tag size = 62 INFO @ Tue, 14 Jul 2020 11:01:20: #1 total tags in treatment: 6969225 INFO @ Tue, 14 Jul 2020 11:01:20: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:01:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:01:20: #1 tags after filtering in treatment: 6969225 INFO @ Tue, 14 Jul 2020 11:01:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:01:20: #1 finished! INFO @ Tue, 14 Jul 2020 11:01:20: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:01:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:01:20: #2 number of paired peaks: 309 WARNING @ Tue, 14 Jul 2020 11:01:20: Fewer paired peaks (309) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 309 pairs to build model! INFO @ Tue, 14 Jul 2020 11:01:20: start model_add_line... INFO @ Tue, 14 Jul 2020 11:01:20: start X-correlation... INFO @ Tue, 14 Jul 2020 11:01:20: end of X-cor INFO @ Tue, 14 Jul 2020 11:01:20: #2 finished! INFO @ Tue, 14 Jul 2020 11:01:20: #2 predicted fragment length is 51 bps INFO @ Tue, 14 Jul 2020 11:01:20: #2 alternative fragment length(s) may be 51,526,556 bps INFO @ Tue, 14 Jul 2020 11:01:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.05_model.r WARNING @ Tue, 14 Jul 2020 11:01:20: #2 Since the d (51) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:01:20: #2 You may need to consider one of the other alternative d(s): 51,526,556 WARNING @ Tue, 14 Jul 2020 11:01:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:01:20: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:01:20: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:01:21: 2000000 INFO @ Tue, 14 Jul 2020 11:01:27: 3000000 INFO @ Tue, 14 Jul 2020 11:01:34: 4000000 INFO @ Tue, 14 Jul 2020 11:01:34: #3 Call peaks for each chromosome... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 11:01:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 11:01:38: #1 read tag files... INFO @ Tue, 14 Jul 2020 11:01:38: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 11:01:40: 5000000 INFO @ Tue, 14 Jul 2020 11:01:41: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.05_peaks.xls INFO @ Tue, 14 Jul 2020 11:01:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:01:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.05_summits.bed INFO @ Tue, 14 Jul 2020 11:01:41: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1802 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:01:45: 1000000 INFO @ Tue, 14 Jul 2020 11:01:46: 6000000 INFO @ Tue, 14 Jul 2020 11:01:51: 2000000 INFO @ Tue, 14 Jul 2020 11:01:52: #1 tag size is determined as 62 bps INFO @ Tue, 14 Jul 2020 11:01:52: #1 tag size = 62 INFO @ Tue, 14 Jul 2020 11:01:52: #1 total tags in treatment: 6969225 INFO @ Tue, 14 Jul 2020 11:01:52: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:01:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:01:52: #1 tags after filtering in treatment: 6969225 INFO @ Tue, 14 Jul 2020 11:01:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:01:52: #1 finished! INFO @ Tue, 14 Jul 2020 11:01:52: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:01:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:01:52: #2 number of paired peaks: 309 WARNING @ Tue, 14 Jul 2020 11:01:52: Fewer paired peaks (309) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 309 pairs to build model! INFO @ Tue, 14 Jul 2020 11:01:52: start model_add_line... INFO @ Tue, 14 Jul 2020 11:01:52: start X-correlation... INFO @ Tue, 14 Jul 2020 11:01:52: end of X-cor INFO @ Tue, 14 Jul 2020 11:01:52: #2 finished! INFO @ Tue, 14 Jul 2020 11:01:52: #2 predicted fragment length is 51 bps INFO @ Tue, 14 Jul 2020 11:01:52: #2 alternative fragment length(s) may be 51,526,556 bps INFO @ Tue, 14 Jul 2020 11:01:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.10_model.r WARNING @ Tue, 14 Jul 2020 11:01:52: #2 Since the d (51) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:01:52: #2 You may need to consider one of the other alternative d(s): 51,526,556 WARNING @ Tue, 14 Jul 2020 11:01:52: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:01:52: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:01:52: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 11:01:57: 3000000 INFO @ Tue, 14 Jul 2020 11:02:03: 4000000 INFO @ Tue, 14 Jul 2020 11:02:06: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:02:09: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 11:02:13: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.10_peaks.xls INFO @ Tue, 14 Jul 2020 11:02:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:02:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.10_summits.bed INFO @ Tue, 14 Jul 2020 11:02:13: Done! pass1 - making usageList (12 chroms): 0 millis pass2 - checking and writing primary data (700 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 11:02:15: 6000000 INFO @ Tue, 14 Jul 2020 11:02:21: #1 tag size is determined as 62 bps INFO @ Tue, 14 Jul 2020 11:02:21: #1 tag size = 62 INFO @ Tue, 14 Jul 2020 11:02:21: #1 total tags in treatment: 6969225 INFO @ Tue, 14 Jul 2020 11:02:21: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 11:02:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 11:02:21: #1 tags after filtering in treatment: 6969225 INFO @ Tue, 14 Jul 2020 11:02:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 11:02:21: #1 finished! INFO @ Tue, 14 Jul 2020 11:02:21: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 11:02:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 11:02:21: #2 number of paired peaks: 309 WARNING @ Tue, 14 Jul 2020 11:02:21: Fewer paired peaks (309) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 309 pairs to build model! INFO @ Tue, 14 Jul 2020 11:02:21: start model_add_line... INFO @ Tue, 14 Jul 2020 11:02:21: start X-correlation... INFO @ Tue, 14 Jul 2020 11:02:21: end of X-cor INFO @ Tue, 14 Jul 2020 11:02:21: #2 finished! INFO @ Tue, 14 Jul 2020 11:02:21: #2 predicted fragment length is 51 bps INFO @ Tue, 14 Jul 2020 11:02:21: #2 alternative fragment length(s) may be 51,526,556 bps INFO @ Tue, 14 Jul 2020 11:02:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.20_model.r WARNING @ Tue, 14 Jul 2020 11:02:21: #2 Since the d (51) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 11:02:21: #2 You may need to consider one of the other alternative d(s): 51,526,556 WARNING @ Tue, 14 Jul 2020 11:02:21: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 11:02:21: #3 Call peaks... INFO @ Tue, 14 Jul 2020 11:02:21: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 11:02:36: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 11:02:43: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.20_peaks.xls INFO @ Tue, 14 Jul 2020 11:02:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 11:02:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521313/SRX8521313.20_summits.bed INFO @ Tue, 14 Jul 2020 11:02:43: Done! pass1 - making usageList (8 chroms): 0 millis pass2 - checking and writing primary data (128 records, 4 fields): 12 millis CompletedMACS2peakCalling