Job ID = 6627152 SRX = SRX8521297 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T01:18:04 prefetch.2.10.7: 1) Downloading 'SRR11978494'... 2020-07-14T01:18:04 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:19:02 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:19:02 prefetch.2.10.7: 'SRR11978494' is valid 2020-07-14T01:19:02 prefetch.2.10.7: 1) 'SRR11978494' was downloaded successfully 2020-07-14T01:19:02 prefetch.2.10.7: 'SRR11978494' has 0 unresolved dependencies Read 4685624 spots for SRR11978494/SRR11978494.sra Written 4685624 spots for SRR11978494/SRR11978494.sra 2020-07-14T01:19:29 prefetch.2.10.7: 1) Downloading 'SRR11978495'... 2020-07-14T01:19:29 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:19:53 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:19:53 prefetch.2.10.7: 'SRR11978495' is valid 2020-07-14T01:19:53 prefetch.2.10.7: 1) 'SRR11978495' was downloaded successfully 2020-07-14T01:19:53 prefetch.2.10.7: 'SRR11978495' has 0 unresolved dependencies Read 2388031 spots for SRR11978495/SRR11978495.sra Written 2388031 spots for SRR11978495/SRR11978495.sra 2020-07-14T01:20:15 prefetch.2.10.7: 1) Downloading 'SRR11978496'... 2020-07-14T01:20:15 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:20:45 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:20:45 prefetch.2.10.7: 'SRR11978496' is valid 2020-07-14T01:20:45 prefetch.2.10.7: 1) 'SRR11978496' was downloaded successfully 2020-07-14T01:20:45 prefetch.2.10.7: 'SRR11978496' has 0 unresolved dependencies Read 2560475 spots for SRR11978496/SRR11978496.sra Written 2560475 spots for SRR11978496/SRR11978496.sra 2020-07-14T01:21:08 prefetch.2.10.7: 1) Downloading 'SRR11978497'... 2020-07-14T01:21:08 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:21:46 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:21:46 prefetch.2.10.7: 'SRR11978497' is valid 2020-07-14T01:21:46 prefetch.2.10.7: 1) 'SRR11978497' was downloaded successfully 2020-07-14T01:21:46 prefetch.2.10.7: 'SRR11978497' has 0 unresolved dependencies Read 2525609 spots for SRR11978497/SRR11978497.sra Written 2525609 spots for SRR11978497/SRR11978497.sra 2020-07-14T01:22:05 prefetch.2.10.7: 1) Downloading 'SRR11978498'... 2020-07-14T01:22:05 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:22:33 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:22:33 prefetch.2.10.7: 'SRR11978498' is valid 2020-07-14T01:22:33 prefetch.2.10.7: 1) 'SRR11978498' was downloaded successfully 2020-07-14T01:22:33 prefetch.2.10.7: 'SRR11978498' has 0 unresolved dependencies Read 3209781 spots for SRR11978498/SRR11978498.sra Written 3209781 spots for SRR11978498/SRR11978498.sra 2020-07-14T01:22:58 prefetch.2.10.7: 1) Downloading 'SRR11978499'... 2020-07-14T01:22:58 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:24:27 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:24:27 prefetch.2.10.7: 'SRR11978499' is valid 2020-07-14T01:24:27 prefetch.2.10.7: 1) 'SRR11978499' was downloaded successfully 2020-07-14T01:24:27 prefetch.2.10.7: 'SRR11978499' has 0 unresolved dependencies Read 3268984 spots for SRR11978499/SRR11978499.sra Written 3268984 spots for SRR11978499/SRR11978499.sra 2020-07-14T01:24:52 prefetch.2.10.7: 1) Downloading 'SRR11978500'... 2020-07-14T01:24:52 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:25:19 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:25:19 prefetch.2.10.7: 'SRR11978500' is valid 2020-07-14T01:25:19 prefetch.2.10.7: 1) 'SRR11978500' was downloaded successfully 2020-07-14T01:25:19 prefetch.2.10.7: 'SRR11978500' has 0 unresolved dependencies Read 3275932 spots for SRR11978500/SRR11978500.sra Written 3275932 spots for SRR11978500/SRR11978500.sra 2020-07-14T01:25:45 prefetch.2.10.7: 1) Downloading 'SRR11978501'... 2020-07-14T01:25:45 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:26:14 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:26:14 prefetch.2.10.7: 'SRR11978501' is valid 2020-07-14T01:26:14 prefetch.2.10.7: 1) 'SRR11978501' was downloaded successfully 2020-07-14T01:26:14 prefetch.2.10.7: 'SRR11978501' has 0 unresolved dependencies Read 3176785 spots for SRR11978501/SRR11978501.sra Written 3176785 spots for SRR11978501/SRR11978501.sra 2020-07-14T01:26:35 prefetch.2.10.7: 1) Downloading 'SRR11978502'... 2020-07-14T01:26:35 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:27:28 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:27:28 prefetch.2.10.7: 'SRR11978502' is valid 2020-07-14T01:27:28 prefetch.2.10.7: 1) 'SRR11978502' was downloaded successfully 2020-07-14T01:27:28 prefetch.2.10.7: 'SRR11978502' has 0 unresolved dependencies Read 4324320 spots for SRR11978502/SRR11978502.sra Written 4324320 spots for SRR11978502/SRR11978502.sra 2020-07-14T01:27:54 prefetch.2.10.7: 1) Downloading 'SRR11978503'... 2020-07-14T01:27:54 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:28:54 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:28:54 prefetch.2.10.7: 'SRR11978503' is valid 2020-07-14T01:28:54 prefetch.2.10.7: 1) 'SRR11978503' was downloaded successfully 2020-07-14T01:28:54 prefetch.2.10.7: 'SRR11978503' has 0 unresolved dependencies Read 4726159 spots for SRR11978503/SRR11978503.sra Written 4726159 spots for SRR11978503/SRR11978503.sra 2020-07-14T01:29:24 prefetch.2.10.7: 1) Downloading 'SRR11978504'... 2020-07-14T01:29:24 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:30:31 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:30:31 prefetch.2.10.7: 'SRR11978504' is valid 2020-07-14T01:30:31 prefetch.2.10.7: 1) 'SRR11978504' was downloaded successfully 2020-07-14T01:30:31 prefetch.2.10.7: 'SRR11978504' has 0 unresolved dependencies Read 4842999 spots for SRR11978504/SRR11978504.sra Written 4842999 spots for SRR11978504/SRR11978504.sra 2020-07-14T01:30:58 prefetch.2.10.7: 1) Downloading 'SRR11978505'... 2020-07-14T01:30:58 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:31:23 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:31:23 prefetch.2.10.7: 'SRR11978505' is valid 2020-07-14T01:31:23 prefetch.2.10.7: 1) 'SRR11978505' was downloaded successfully 2020-07-14T01:31:23 prefetch.2.10.7: 'SRR11978505' has 0 unresolved dependencies Read 1032316 spots for SRR11978505/SRR11978505.sra Written 1032316 spots for SRR11978505/SRR11978505.sra 2020-07-14T01:31:46 prefetch.2.10.7: 1) Downloading 'SRR11978506'... 2020-07-14T01:31:46 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:32:12 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:32:12 prefetch.2.10.7: 'SRR11978506' is valid 2020-07-14T01:32:12 prefetch.2.10.7: 1) 'SRR11978506' was downloaded successfully 2020-07-14T01:32:12 prefetch.2.10.7: 'SRR11978506' has 0 unresolved dependencies Read 974578 spots for SRR11978506/SRR11978506.sra Written 974578 spots for SRR11978506/SRR11978506.sra 2020-07-14T01:32:30 prefetch.2.10.7: 1) Downloading 'SRR11978507'... 2020-07-14T01:32:30 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:33:02 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:33:02 prefetch.2.10.7: 'SRR11978507' is valid 2020-07-14T01:33:02 prefetch.2.10.7: 1) 'SRR11978507' was downloaded successfully 2020-07-14T01:33:02 prefetch.2.10.7: 'SRR11978507' has 0 unresolved dependencies Read 1007758 spots for SRR11978507/SRR11978507.sra Written 1007758 spots for SRR11978507/SRR11978507.sra 2020-07-14T01:33:16 prefetch.2.10.7: 1) Downloading 'SRR11978508'... 2020-07-14T01:33:16 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:33:41 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:33:41 prefetch.2.10.7: 'SRR11978508' is valid 2020-07-14T01:33:42 prefetch.2.10.7: 1) 'SRR11978508' was downloaded successfully 2020-07-14T01:33:42 prefetch.2.10.7: 'SRR11978508' has 0 unresolved dependencies Read 1021778 spots for SRR11978508/SRR11978508.sra Written 1021778 spots for SRR11978508/SRR11978508.sra 2020-07-14T01:33:59 prefetch.2.10.7: 1) Downloading 'SRR11978509'... 2020-07-14T01:33:59 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T01:34:31 prefetch.2.10.7: HTTPS download succeed 2020-07-14T01:34:32 prefetch.2.10.7: 'SRR11978509' is valid 2020-07-14T01:34:32 prefetch.2.10.7: 1) 'SRR11978509' was downloaded successfully 2020-07-14T01:34:32 prefetch.2.10.7: 'SRR11978509' has 0 unresolved dependencies Read 2542127 spots for SRR11978509/SRR11978509.sra Written 2542127 spots for SRR11978509/SRR11978509.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627429 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:11 45563256 reads; of these: 45563256 (100.00%) were unpaired; of these: 36583015 (80.29%) aligned 0 times 6586215 (14.46%) aligned exactly 1 time 2394026 (5.25%) aligned >1 times 19.71% overall alignment rate Time searching: 00:09:12 Overall time: 00:09:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1657231 / 8980241 = 0.1845 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:47:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:47:27: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:47:27: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:47:33: 1000000 INFO @ Tue, 14 Jul 2020 10:47:39: 2000000 INFO @ Tue, 14 Jul 2020 10:47:45: 3000000 INFO @ Tue, 14 Jul 2020 10:47:51: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:47:57: 5000000 INFO @ Tue, 14 Jul 2020 10:47:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:47:57: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:47:57: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:48:03: 6000000 INFO @ Tue, 14 Jul 2020 10:48:03: 1000000 INFO @ Tue, 14 Jul 2020 10:48:08: 2000000 INFO @ Tue, 14 Jul 2020 10:48:09: 7000000 INFO @ Tue, 14 Jul 2020 10:48:11: #1 tag size is determined as 63 bps INFO @ Tue, 14 Jul 2020 10:48:11: #1 tag size = 63 INFO @ Tue, 14 Jul 2020 10:48:11: #1 total tags in treatment: 7323010 INFO @ Tue, 14 Jul 2020 10:48:11: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:48:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:48:11: #1 tags after filtering in treatment: 7323010 INFO @ Tue, 14 Jul 2020 10:48:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:48:11: #1 finished! INFO @ Tue, 14 Jul 2020 10:48:11: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:48:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:48:11: #2 number of paired peaks: 335 WARNING @ Tue, 14 Jul 2020 10:48:11: Fewer paired peaks (335) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 335 pairs to build model! INFO @ Tue, 14 Jul 2020 10:48:11: start model_add_line... INFO @ Tue, 14 Jul 2020 10:48:11: start X-correlation... INFO @ Tue, 14 Jul 2020 10:48:11: end of X-cor INFO @ Tue, 14 Jul 2020 10:48:11: #2 finished! INFO @ Tue, 14 Jul 2020 10:48:11: #2 predicted fragment length is 60 bps INFO @ Tue, 14 Jul 2020 10:48:11: #2 alternative fragment length(s) may be 60 bps INFO @ Tue, 14 Jul 2020 10:48:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.05_model.r WARNING @ Tue, 14 Jul 2020 10:48:11: #2 Since the d (60) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 10:48:11: #2 You may need to consider one of the other alternative d(s): 60 WARNING @ Tue, 14 Jul 2020 10:48:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 10:48:11: #3 Call peaks... INFO @ Tue, 14 Jul 2020 10:48:11: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 10:48:14: 3000000 INFO @ Tue, 14 Jul 2020 10:48:19: 4000000 INFO @ Tue, 14 Jul 2020 10:48:25: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:48:26: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 10:48:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:48:27: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:48:27: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:48:30: 6000000 INFO @ Tue, 14 Jul 2020 10:48:33: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.05_peaks.xls INFO @ Tue, 14 Jul 2020 10:48:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 10:48:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.05_summits.bed INFO @ Tue, 14 Jul 2020 10:48:33: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (2657 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:48:35: 1000000 INFO @ Tue, 14 Jul 2020 10:48:36: 7000000 INFO @ Tue, 14 Jul 2020 10:48:37: #1 tag size is determined as 63 bps INFO @ Tue, 14 Jul 2020 10:48:37: #1 tag size = 63 INFO @ Tue, 14 Jul 2020 10:48:37: #1 total tags in treatment: 7323010 INFO @ Tue, 14 Jul 2020 10:48:37: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:48:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:48:37: #1 tags after filtering in treatment: 7323010 INFO @ Tue, 14 Jul 2020 10:48:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:48:37: #1 finished! INFO @ Tue, 14 Jul 2020 10:48:37: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:48:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:48:38: #2 number of paired peaks: 335 WARNING @ Tue, 14 Jul 2020 10:48:38: Fewer paired peaks (335) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 335 pairs to build model! INFO @ Tue, 14 Jul 2020 10:48:38: start model_add_line... INFO @ Tue, 14 Jul 2020 10:48:38: start X-correlation... INFO @ Tue, 14 Jul 2020 10:48:38: end of X-cor INFO @ Tue, 14 Jul 2020 10:48:38: #2 finished! INFO @ Tue, 14 Jul 2020 10:48:38: #2 predicted fragment length is 60 bps INFO @ Tue, 14 Jul 2020 10:48:38: #2 alternative fragment length(s) may be 60 bps INFO @ Tue, 14 Jul 2020 10:48:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.10_model.r WARNING @ Tue, 14 Jul 2020 10:48:38: #2 Since the d (60) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 10:48:38: #2 You may need to consider one of the other alternative d(s): 60 WARNING @ Tue, 14 Jul 2020 10:48:38: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 10:48:38: #3 Call peaks... INFO @ Tue, 14 Jul 2020 10:48:38: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 10:48:43: 2000000 INFO @ Tue, 14 Jul 2020 10:48:50: 3000000 INFO @ Tue, 14 Jul 2020 10:48:53: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 10:48:57: 4000000 INFO @ Tue, 14 Jul 2020 10:49:01: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.10_peaks.xls INFO @ Tue, 14 Jul 2020 10:49:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 10:49:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.10_summits.bed INFO @ Tue, 14 Jul 2020 10:49:01: Done! pass1 - making usageList (14 chroms): 0 millis pass2 - checking and writing primary data (930 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:49:03: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 10:49:10: 6000000 INFO @ Tue, 14 Jul 2020 10:49:17: 7000000 INFO @ Tue, 14 Jul 2020 10:49:20: #1 tag size is determined as 63 bps INFO @ Tue, 14 Jul 2020 10:49:20: #1 tag size = 63 INFO @ Tue, 14 Jul 2020 10:49:20: #1 total tags in treatment: 7323010 INFO @ Tue, 14 Jul 2020 10:49:20: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:49:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:49:20: #1 tags after filtering in treatment: 7323010 INFO @ Tue, 14 Jul 2020 10:49:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 10:49:20: #1 finished! INFO @ Tue, 14 Jul 2020 10:49:20: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:49:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:49:20: #2 number of paired peaks: 335 WARNING @ Tue, 14 Jul 2020 10:49:20: Fewer paired peaks (335) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 335 pairs to build model! INFO @ Tue, 14 Jul 2020 10:49:20: start model_add_line... INFO @ Tue, 14 Jul 2020 10:49:20: start X-correlation... INFO @ Tue, 14 Jul 2020 10:49:20: end of X-cor INFO @ Tue, 14 Jul 2020 10:49:20: #2 finished! INFO @ Tue, 14 Jul 2020 10:49:20: #2 predicted fragment length is 60 bps INFO @ Tue, 14 Jul 2020 10:49:20: #2 alternative fragment length(s) may be 60 bps INFO @ Tue, 14 Jul 2020 10:49:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.20_model.r WARNING @ Tue, 14 Jul 2020 10:49:20: #2 Since the d (60) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 10:49:20: #2 You may need to consider one of the other alternative d(s): 60 WARNING @ Tue, 14 Jul 2020 10:49:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 10:49:20: #3 Call peaks... INFO @ Tue, 14 Jul 2020 10:49:20: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 10:49:36: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 10:49:45: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.20_peaks.xls INFO @ Tue, 14 Jul 2020 10:49:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 10:49:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX8521297/SRX8521297.20_summits.bed INFO @ Tue, 14 Jul 2020 10:49:45: Done! pass1 - making usageList (8 chroms): 1 millis pass2 - checking and writing primary data (212 records, 4 fields): 1 millis CompletedMACS2peakCalling