Job ID = 16439716 SRX = SRX8369901 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 79886931 spots for SRR11818937/SRR11818937.sra Written 79886931 spots for SRR11818937/SRR11818937.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440011 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:28:28 79886931 reads; of these: 79886931 (100.00%) were unpaired; of these: 4390859 (5.50%) aligned 0 times 52145652 (65.27%) aligned exactly 1 time 23350420 (29.23%) aligned >1 times 94.50% overall alignment rate Time searching: 00:28:28 Overall time: 00:28:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 28450558 / 75496072 = 0.3768 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:44:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:44:33: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:44:33: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:44:39: 1000000 INFO @ Tue, 02 Aug 2022 16:44:44: 2000000 INFO @ Tue, 02 Aug 2022 16:44:50: 3000000 INFO @ Tue, 02 Aug 2022 16:44:55: 4000000 INFO @ Tue, 02 Aug 2022 16:45:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:45:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:45:04: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:45:04: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:45:07: 6000000 INFO @ Tue, 02 Aug 2022 16:45:10: 1000000 INFO @ Tue, 02 Aug 2022 16:45:12: 7000000 INFO @ Tue, 02 Aug 2022 16:45:16: 2000000 INFO @ Tue, 02 Aug 2022 16:45:18: 8000000 INFO @ Tue, 02 Aug 2022 16:45:22: 3000000 INFO @ Tue, 02 Aug 2022 16:45:24: 9000000 INFO @ Tue, 02 Aug 2022 16:45:28: 4000000 INFO @ Tue, 02 Aug 2022 16:45:30: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:45:33: 5000000 INFO @ Tue, 02 Aug 2022 16:45:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:45:33: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:45:33: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:45:36: 11000000 INFO @ Tue, 02 Aug 2022 16:45:39: 1000000 INFO @ Tue, 02 Aug 2022 16:45:39: 6000000 INFO @ Tue, 02 Aug 2022 16:45:41: 12000000 INFO @ Tue, 02 Aug 2022 16:45:44: 2000000 INFO @ Tue, 02 Aug 2022 16:45:45: 7000000 INFO @ Tue, 02 Aug 2022 16:45:47: 13000000 INFO @ Tue, 02 Aug 2022 16:45:50: 3000000 INFO @ Tue, 02 Aug 2022 16:45:51: 8000000 INFO @ Tue, 02 Aug 2022 16:45:53: 14000000 INFO @ Tue, 02 Aug 2022 16:45:55: 4000000 INFO @ Tue, 02 Aug 2022 16:45:57: 9000000 INFO @ Tue, 02 Aug 2022 16:45:59: 15000000 INFO @ Tue, 02 Aug 2022 16:46:00: 5000000 INFO @ Tue, 02 Aug 2022 16:46:03: 10000000 INFO @ Tue, 02 Aug 2022 16:46:05: 16000000 INFO @ Tue, 02 Aug 2022 16:46:06: 6000000 INFO @ Tue, 02 Aug 2022 16:46:09: 11000000 INFO @ Tue, 02 Aug 2022 16:46:11: 17000000 INFO @ Tue, 02 Aug 2022 16:46:11: 7000000 INFO @ Tue, 02 Aug 2022 16:46:15: 12000000 INFO @ Tue, 02 Aug 2022 16:46:16: 18000000 INFO @ Tue, 02 Aug 2022 16:46:16: 8000000 INFO @ Tue, 02 Aug 2022 16:46:21: 13000000 INFO @ Tue, 02 Aug 2022 16:46:22: 9000000 INFO @ Tue, 02 Aug 2022 16:46:22: 19000000 INFO @ Tue, 02 Aug 2022 16:46:27: 14000000 INFO @ Tue, 02 Aug 2022 16:46:27: 10000000 INFO @ Tue, 02 Aug 2022 16:46:28: 20000000 INFO @ Tue, 02 Aug 2022 16:46:32: 11000000 INFO @ Tue, 02 Aug 2022 16:46:33: 15000000 INFO @ Tue, 02 Aug 2022 16:46:34: 21000000 INFO @ Tue, 02 Aug 2022 16:46:38: 12000000 INFO @ Tue, 02 Aug 2022 16:46:39: 16000000 INFO @ Tue, 02 Aug 2022 16:46:40: 22000000 INFO @ Tue, 02 Aug 2022 16:46:43: 13000000 INFO @ Tue, 02 Aug 2022 16:46:45: 17000000 INFO @ Tue, 02 Aug 2022 16:46:45: 23000000 INFO @ Tue, 02 Aug 2022 16:46:48: 14000000 INFO @ Tue, 02 Aug 2022 16:46:51: 18000000 INFO @ Tue, 02 Aug 2022 16:46:51: 24000000 INFO @ Tue, 02 Aug 2022 16:46:54: 15000000 INFO @ Tue, 02 Aug 2022 16:46:57: 19000000 INFO @ Tue, 02 Aug 2022 16:46:57: 25000000 INFO @ Tue, 02 Aug 2022 16:46:59: 16000000 INFO @ Tue, 02 Aug 2022 16:47:03: 20000000 INFO @ Tue, 02 Aug 2022 16:47:03: 26000000 INFO @ Tue, 02 Aug 2022 16:47:04: 17000000 INFO @ Tue, 02 Aug 2022 16:47:08: 21000000 INFO @ Tue, 02 Aug 2022 16:47:09: 27000000 INFO @ Tue, 02 Aug 2022 16:47:10: 18000000 INFO @ Tue, 02 Aug 2022 16:47:14: 22000000 INFO @ Tue, 02 Aug 2022 16:47:15: 19000000 INFO @ Tue, 02 Aug 2022 16:47:15: 28000000 INFO @ Tue, 02 Aug 2022 16:47:20: 23000000 INFO @ Tue, 02 Aug 2022 16:47:20: 20000000 INFO @ Tue, 02 Aug 2022 16:47:21: 29000000 INFO @ Tue, 02 Aug 2022 16:47:26: 21000000 INFO @ Tue, 02 Aug 2022 16:47:26: 24000000 INFO @ Tue, 02 Aug 2022 16:47:27: 30000000 INFO @ Tue, 02 Aug 2022 16:47:31: 22000000 INFO @ Tue, 02 Aug 2022 16:47:32: 25000000 INFO @ Tue, 02 Aug 2022 16:47:33: 31000000 INFO @ Tue, 02 Aug 2022 16:47:36: 23000000 INFO @ Tue, 02 Aug 2022 16:47:38: 26000000 INFO @ Tue, 02 Aug 2022 16:47:39: 32000000 INFO @ Tue, 02 Aug 2022 16:47:42: 24000000 INFO @ Tue, 02 Aug 2022 16:47:44: 27000000 INFO @ Tue, 02 Aug 2022 16:47:45: 33000000 INFO @ Tue, 02 Aug 2022 16:47:47: 25000000 INFO @ Tue, 02 Aug 2022 16:47:50: 28000000 INFO @ Tue, 02 Aug 2022 16:47:50: 34000000 INFO @ Tue, 02 Aug 2022 16:47:52: 26000000 INFO @ Tue, 02 Aug 2022 16:47:56: 29000000 INFO @ Tue, 02 Aug 2022 16:47:56: 35000000 INFO @ Tue, 02 Aug 2022 16:47:57: 27000000 INFO @ Tue, 02 Aug 2022 16:48:02: 30000000 INFO @ Tue, 02 Aug 2022 16:48:02: 36000000 INFO @ Tue, 02 Aug 2022 16:48:03: 28000000 INFO @ Tue, 02 Aug 2022 16:48:07: 31000000 INFO @ Tue, 02 Aug 2022 16:48:08: 29000000 INFO @ Tue, 02 Aug 2022 16:48:08: 37000000 INFO @ Tue, 02 Aug 2022 16:48:13: 30000000 INFO @ Tue, 02 Aug 2022 16:48:13: 32000000 INFO @ Tue, 02 Aug 2022 16:48:14: 38000000 INFO @ Tue, 02 Aug 2022 16:48:19: 31000000 INFO @ Tue, 02 Aug 2022 16:48:19: 33000000 INFO @ Tue, 02 Aug 2022 16:48:20: 39000000 INFO @ Tue, 02 Aug 2022 16:48:24: 32000000 INFO @ Tue, 02 Aug 2022 16:48:25: 34000000 INFO @ Tue, 02 Aug 2022 16:48:26: 40000000 INFO @ Tue, 02 Aug 2022 16:48:29: 33000000 INFO @ Tue, 02 Aug 2022 16:48:31: 35000000 INFO @ Tue, 02 Aug 2022 16:48:32: 41000000 INFO @ Tue, 02 Aug 2022 16:48:34: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:48:37: 36000000 INFO @ Tue, 02 Aug 2022 16:48:38: 42000000 INFO @ Tue, 02 Aug 2022 16:48:40: 35000000 INFO @ Tue, 02 Aug 2022 16:48:43: 37000000 INFO @ Tue, 02 Aug 2022 16:48:44: 43000000 INFO @ Tue, 02 Aug 2022 16:48:46: 36000000 INFO @ Tue, 02 Aug 2022 16:48:50: 38000000 INFO @ Tue, 02 Aug 2022 16:48:50: 44000000 INFO @ Tue, 02 Aug 2022 16:48:51: 37000000 INFO @ Tue, 02 Aug 2022 16:48:56: 39000000 INFO @ Tue, 02 Aug 2022 16:48:56: 45000000 INFO @ Tue, 02 Aug 2022 16:48:56: 38000000 INFO @ Tue, 02 Aug 2022 16:49:02: 39000000 INFO @ Tue, 02 Aug 2022 16:49:02: 46000000 INFO @ Tue, 02 Aug 2022 16:49:02: 40000000 INFO @ Tue, 02 Aug 2022 16:49:07: 40000000 INFO @ Tue, 02 Aug 2022 16:49:08: 47000000 INFO @ Tue, 02 Aug 2022 16:49:08: 41000000 INFO @ Tue, 02 Aug 2022 16:49:08: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 16:49:08: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 16:49:08: #1 total tags in treatment: 47045514 INFO @ Tue, 02 Aug 2022 16:49:08: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:49:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:49:09: #1 tags after filtering in treatment: 47045514 INFO @ Tue, 02 Aug 2022 16:49:09: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:49:09: #1 finished! INFO @ Tue, 02 Aug 2022 16:49:09: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:49:09: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:49:12: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 16:49:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:49:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.05_peaks.narrowPeak: No such file or directory INFO @ Tue, 02 Aug 2022 16:49:12: 41000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:49:14: 42000000 INFO @ Tue, 02 Aug 2022 16:49:18: 42000000 INFO @ Tue, 02 Aug 2022 16:49:20: 43000000 INFO @ Tue, 02 Aug 2022 16:49:24: 43000000 INFO @ Tue, 02 Aug 2022 16:49:26: 44000000 INFO @ Tue, 02 Aug 2022 16:49:29: 44000000 INFO @ Tue, 02 Aug 2022 16:49:32: 45000000 INFO @ Tue, 02 Aug 2022 16:49:34: 45000000 INFO @ Tue, 02 Aug 2022 16:49:38: 46000000 INFO @ Tue, 02 Aug 2022 16:49:39: 46000000 INFO @ Tue, 02 Aug 2022 16:49:44: 47000000 INFO @ Tue, 02 Aug 2022 16:49:44: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 16:49:44: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 16:49:44: #1 total tags in treatment: 47045514 INFO @ Tue, 02 Aug 2022 16:49:44: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:49:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:49:45: 47000000 INFO @ Tue, 02 Aug 2022 16:49:45: #1 tags after filtering in treatment: 47045514 INFO @ Tue, 02 Aug 2022 16:49:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:49:45: #1 finished! INFO @ Tue, 02 Aug 2022 16:49:45: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:49:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:49:45: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 16:49:45: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 16:49:45: #1 total tags in treatment: 47045514 INFO @ Tue, 02 Aug 2022 16:49:45: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:49:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:49:46: #1 tags after filtering in treatment: 47045514 INFO @ Tue, 02 Aug 2022 16:49:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:49:46: #1 finished! INFO @ Tue, 02 Aug 2022 16:49:46: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:49:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:49:48: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 16:49:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:49:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:49:49: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 16:49:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:49:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369901/SRX8369901.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。