Job ID = 16439809 SRX = SRX8369898 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 42790523 spots for SRR11818934/SRR11818934.sra Written 42790523 spots for SRR11818934/SRR11818934.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439999 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:15:06 42790523 reads; of these: 42790523 (100.00%) were unpaired; of these: 6531959 (15.26%) aligned 0 times 28101355 (65.67%) aligned exactly 1 time 8157209 (19.06%) aligned >1 times 84.74% overall alignment rate Time searching: 00:15:07 Overall time: 00:15:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7100719 / 36258564 = 0.1958 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:40:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:40:04: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:40:04: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:40:12: 1000000 INFO @ Tue, 02 Aug 2022 16:40:20: 2000000 INFO @ Tue, 02 Aug 2022 16:40:28: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:40:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:40:32: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:40:32: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:40:35: 4000000 INFO @ Tue, 02 Aug 2022 16:40:42: 1000000 INFO @ Tue, 02 Aug 2022 16:40:43: 5000000 INFO @ Tue, 02 Aug 2022 16:40:50: 6000000 INFO @ Tue, 02 Aug 2022 16:40:52: 2000000 INFO @ Tue, 02 Aug 2022 16:40:57: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:41:01: 3000000 INFO @ Tue, 02 Aug 2022 16:41:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:41:02: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:41:02: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:41:05: 8000000 INFO @ Tue, 02 Aug 2022 16:41:12: 4000000 INFO @ Tue, 02 Aug 2022 16:41:12: 1000000 INFO @ Tue, 02 Aug 2022 16:41:13: 9000000 INFO @ Tue, 02 Aug 2022 16:41:20: 2000000 INFO @ Tue, 02 Aug 2022 16:41:20: 10000000 INFO @ Tue, 02 Aug 2022 16:41:22: 5000000 INFO @ Tue, 02 Aug 2022 16:41:28: 11000000 INFO @ Tue, 02 Aug 2022 16:41:29: 3000000 INFO @ Tue, 02 Aug 2022 16:41:32: 6000000 INFO @ Tue, 02 Aug 2022 16:41:36: 12000000 INFO @ Tue, 02 Aug 2022 16:41:37: 4000000 INFO @ Tue, 02 Aug 2022 16:41:42: 7000000 INFO @ Tue, 02 Aug 2022 16:41:44: 13000000 INFO @ Tue, 02 Aug 2022 16:41:45: 5000000 INFO @ Tue, 02 Aug 2022 16:41:52: 14000000 INFO @ Tue, 02 Aug 2022 16:41:52: 8000000 INFO @ Tue, 02 Aug 2022 16:41:53: 6000000 INFO @ Tue, 02 Aug 2022 16:41:59: 15000000 INFO @ Tue, 02 Aug 2022 16:42:01: 7000000 INFO @ Tue, 02 Aug 2022 16:42:02: 9000000 INFO @ Tue, 02 Aug 2022 16:42:08: 16000000 INFO @ Tue, 02 Aug 2022 16:42:09: 8000000 INFO @ Tue, 02 Aug 2022 16:42:13: 10000000 INFO @ Tue, 02 Aug 2022 16:42:17: 17000000 INFO @ Tue, 02 Aug 2022 16:42:17: 9000000 INFO @ Tue, 02 Aug 2022 16:42:23: 11000000 INFO @ Tue, 02 Aug 2022 16:42:25: 18000000 INFO @ Tue, 02 Aug 2022 16:42:25: 10000000 INFO @ Tue, 02 Aug 2022 16:42:33: 11000000 INFO @ Tue, 02 Aug 2022 16:42:33: 12000000 INFO @ Tue, 02 Aug 2022 16:42:33: 19000000 INFO @ Tue, 02 Aug 2022 16:42:40: 12000000 INFO @ Tue, 02 Aug 2022 16:42:41: 20000000 INFO @ Tue, 02 Aug 2022 16:42:43: 13000000 INFO @ Tue, 02 Aug 2022 16:42:49: 13000000 INFO @ Tue, 02 Aug 2022 16:42:50: 21000000 INFO @ Tue, 02 Aug 2022 16:42:53: 14000000 INFO @ Tue, 02 Aug 2022 16:42:56: 14000000 INFO @ Tue, 02 Aug 2022 16:42:58: 22000000 INFO @ Tue, 02 Aug 2022 16:43:03: 15000000 INFO @ Tue, 02 Aug 2022 16:43:05: 15000000 INFO @ Tue, 02 Aug 2022 16:43:07: 23000000 INFO @ Tue, 02 Aug 2022 16:43:14: 16000000 INFO @ Tue, 02 Aug 2022 16:43:14: 16000000 INFO @ Tue, 02 Aug 2022 16:43:15: 24000000 INFO @ Tue, 02 Aug 2022 16:43:23: 17000000 INFO @ Tue, 02 Aug 2022 16:43:24: 17000000 INFO @ Tue, 02 Aug 2022 16:43:24: 25000000 INFO @ Tue, 02 Aug 2022 16:43:32: 18000000 INFO @ Tue, 02 Aug 2022 16:43:32: 26000000 INFO @ Tue, 02 Aug 2022 16:43:34: 18000000 INFO @ Tue, 02 Aug 2022 16:43:40: 19000000 INFO @ Tue, 02 Aug 2022 16:43:41: 27000000 INFO @ Tue, 02 Aug 2022 16:43:44: 19000000 INFO @ Tue, 02 Aug 2022 16:43:48: 20000000 INFO @ Tue, 02 Aug 2022 16:43:49: 28000000 INFO @ Tue, 02 Aug 2022 16:43:54: 20000000 INFO @ Tue, 02 Aug 2022 16:43:58: 21000000 INFO @ Tue, 02 Aug 2022 16:43:58: 29000000 INFO @ Tue, 02 Aug 2022 16:44:00: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 16:44:00: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 16:44:00: #1 total tags in treatment: 29157845 INFO @ Tue, 02 Aug 2022 16:44:00: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:44:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:44:01: #1 tags after filtering in treatment: 29157845 INFO @ Tue, 02 Aug 2022 16:44:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:44:01: #1 finished! INFO @ Tue, 02 Aug 2022 16:44:01: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:44:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:44:03: #2 number of paired peaks: 77 WARNING @ Tue, 02 Aug 2022 16:44:03: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:44:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.05_peaks.narrowPeak: No such file or directory INFO @ Tue, 02 Aug 2022 16:44:04: 21000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:44:06: 22000000 INFO @ Tue, 02 Aug 2022 16:44:14: 22000000 INFO @ Tue, 02 Aug 2022 16:44:15: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:44:23: 24000000 INFO @ Tue, 02 Aug 2022 16:44:24: 23000000 INFO @ Tue, 02 Aug 2022 16:44:31: 25000000 INFO @ Tue, 02 Aug 2022 16:44:35: 24000000 INFO @ Tue, 02 Aug 2022 16:44:39: 26000000 INFO @ Tue, 02 Aug 2022 16:44:45: 25000000 INFO @ Tue, 02 Aug 2022 16:44:47: 27000000 INFO @ Tue, 02 Aug 2022 16:44:54: 26000000 INFO @ Tue, 02 Aug 2022 16:44:55: 28000000 INFO @ Tue, 02 Aug 2022 16:45:03: 29000000 INFO @ Tue, 02 Aug 2022 16:45:04: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 16:45:04: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 16:45:04: #1 total tags in treatment: 29157845 INFO @ Tue, 02 Aug 2022 16:45:04: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:45:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:45:04: 27000000 INFO @ Tue, 02 Aug 2022 16:45:05: #1 tags after filtering in treatment: 29157845 INFO @ Tue, 02 Aug 2022 16:45:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:45:05: #1 finished! INFO @ Tue, 02 Aug 2022 16:45:05: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:45:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:45:07: #2 number of paired peaks: 77 WARNING @ Tue, 02 Aug 2022 16:45:07: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:45:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:45:14: 28000000 INFO @ Tue, 02 Aug 2022 16:45:24: 29000000 INFO @ Tue, 02 Aug 2022 16:45:26: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 16:45:26: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 16:45:26: #1 total tags in treatment: 29157845 INFO @ Tue, 02 Aug 2022 16:45:26: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:45:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:45:27: #1 tags after filtering in treatment: 29157845 INFO @ Tue, 02 Aug 2022 16:45:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:45:27: #1 finished! INFO @ Tue, 02 Aug 2022 16:45:27: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:45:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:45:29: #2 number of paired peaks: 77 WARNING @ Tue, 02 Aug 2022 16:45:29: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 16:45:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8369898/SRX8369898.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。