Job ID = 14170527 SRX = SRX8174954 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 71023950 spots for SRR11608682/SRR11608682.sra Written 71023950 spots for SRR11608682/SRR11608682.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171032 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:09 71023950 reads; of these: 71023950 (100.00%) were unpaired; of these: 9779685 (13.77%) aligned 0 times 53204909 (74.91%) aligned exactly 1 time 8039356 (11.32%) aligned >1 times 86.23% overall alignment rate Time searching: 00:27:10 Overall time: 00:27:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 13838759 / 61244265 = 0.2260 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:58:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:58:02: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:58:02: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:58:14: 1000000 INFO @ Sat, 11 Dec 2021 07:58:25: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:58:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:58:32: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:58:32: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:58:36: 3000000 INFO @ Sat, 11 Dec 2021 07:58:43: 1000000 INFO @ Sat, 11 Dec 2021 07:58:48: 4000000 INFO @ Sat, 11 Dec 2021 07:58:54: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:58:59: 5000000 INFO @ Sat, 11 Dec 2021 07:59:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:59:02: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:59:02: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:59:05: 3000000 INFO @ Sat, 11 Dec 2021 07:59:11: 6000000 INFO @ Sat, 11 Dec 2021 07:59:15: 1000000 INFO @ Sat, 11 Dec 2021 07:59:17: 4000000 INFO @ Sat, 11 Dec 2021 07:59:22: 7000000 INFO @ Sat, 11 Dec 2021 07:59:26: 2000000 INFO @ Sat, 11 Dec 2021 07:59:28: 5000000 INFO @ Sat, 11 Dec 2021 07:59:34: 8000000 INFO @ Sat, 11 Dec 2021 07:59:38: 3000000 INFO @ Sat, 11 Dec 2021 07:59:39: 6000000 INFO @ Sat, 11 Dec 2021 07:59:46: 9000000 INFO @ Sat, 11 Dec 2021 07:59:50: 7000000 INFO @ Sat, 11 Dec 2021 07:59:50: 4000000 INFO @ Sat, 11 Dec 2021 07:59:57: 10000000 INFO @ Sat, 11 Dec 2021 08:00:02: 8000000 INFO @ Sat, 11 Dec 2021 08:00:03: 5000000 INFO @ Sat, 11 Dec 2021 08:00:09: 11000000 INFO @ Sat, 11 Dec 2021 08:00:14: 9000000 INFO @ Sat, 11 Dec 2021 08:00:15: 6000000 INFO @ Sat, 11 Dec 2021 08:00:20: 12000000 INFO @ Sat, 11 Dec 2021 08:00:25: 10000000 INFO @ Sat, 11 Dec 2021 08:00:27: 7000000 INFO @ Sat, 11 Dec 2021 08:00:32: 13000000 INFO @ Sat, 11 Dec 2021 08:00:36: 11000000 INFO @ Sat, 11 Dec 2021 08:00:39: 8000000 INFO @ Sat, 11 Dec 2021 08:00:43: 14000000 INFO @ Sat, 11 Dec 2021 08:00:47: 12000000 INFO @ Sat, 11 Dec 2021 08:00:51: 9000000 INFO @ Sat, 11 Dec 2021 08:00:54: 15000000 INFO @ Sat, 11 Dec 2021 08:00:58: 13000000 INFO @ Sat, 11 Dec 2021 08:01:03: 10000000 INFO @ Sat, 11 Dec 2021 08:01:06: 16000000 INFO @ Sat, 11 Dec 2021 08:01:10: 14000000 INFO @ Sat, 11 Dec 2021 08:01:15: 11000000 INFO @ Sat, 11 Dec 2021 08:01:18: 17000000 INFO @ Sat, 11 Dec 2021 08:01:21: 15000000 INFO @ Sat, 11 Dec 2021 08:01:27: 12000000 INFO @ Sat, 11 Dec 2021 08:01:29: 18000000 INFO @ Sat, 11 Dec 2021 08:01:32: 16000000 INFO @ Sat, 11 Dec 2021 08:01:39: 13000000 INFO @ Sat, 11 Dec 2021 08:01:41: 19000000 INFO @ Sat, 11 Dec 2021 08:01:43: 17000000 INFO @ Sat, 11 Dec 2021 08:01:50: 14000000 INFO @ Sat, 11 Dec 2021 08:01:53: 20000000 INFO @ Sat, 11 Dec 2021 08:01:55: 18000000 INFO @ Sat, 11 Dec 2021 08:02:02: 15000000 INFO @ Sat, 11 Dec 2021 08:02:05: 21000000 INFO @ Sat, 11 Dec 2021 08:02:07: 19000000 INFO @ Sat, 11 Dec 2021 08:02:14: 16000000 INFO @ Sat, 11 Dec 2021 08:02:16: 22000000 INFO @ Sat, 11 Dec 2021 08:02:18: 20000000 INFO @ Sat, 11 Dec 2021 08:02:26: 17000000 INFO @ Sat, 11 Dec 2021 08:02:28: 23000000 INFO @ Sat, 11 Dec 2021 08:02:29: 21000000 INFO @ Sat, 11 Dec 2021 08:02:38: 18000000 INFO @ Sat, 11 Dec 2021 08:02:40: 24000000 INFO @ Sat, 11 Dec 2021 08:02:41: 22000000 INFO @ Sat, 11 Dec 2021 08:02:50: 19000000 INFO @ Sat, 11 Dec 2021 08:02:51: 25000000 INFO @ Sat, 11 Dec 2021 08:02:52: 23000000 INFO @ Sat, 11 Dec 2021 08:03:02: 20000000 INFO @ Sat, 11 Dec 2021 08:03:03: 26000000 INFO @ Sat, 11 Dec 2021 08:03:04: 24000000 INFO @ Sat, 11 Dec 2021 08:03:14: 21000000 INFO @ Sat, 11 Dec 2021 08:03:14: 27000000 INFO @ Sat, 11 Dec 2021 08:03:16: 25000000 INFO @ Sat, 11 Dec 2021 08:03:26: 28000000 INFO @ Sat, 11 Dec 2021 08:03:26: 22000000 INFO @ Sat, 11 Dec 2021 08:03:27: 26000000 INFO @ Sat, 11 Dec 2021 08:03:38: 29000000 INFO @ Sat, 11 Dec 2021 08:03:38: 23000000 INFO @ Sat, 11 Dec 2021 08:03:38: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 08:03:49: 30000000 INFO @ Sat, 11 Dec 2021 08:03:50: 28000000 INFO @ Sat, 11 Dec 2021 08:03:50: 24000000 INFO @ Sat, 11 Dec 2021 08:04:01: 31000000 INFO @ Sat, 11 Dec 2021 08:04:01: 29000000 INFO @ Sat, 11 Dec 2021 08:04:02: 25000000 INFO @ Sat, 11 Dec 2021 08:04:12: 32000000 INFO @ Sat, 11 Dec 2021 08:04:13: 30000000 INFO @ Sat, 11 Dec 2021 08:04:14: 26000000 INFO @ Sat, 11 Dec 2021 08:04:24: 33000000 INFO @ Sat, 11 Dec 2021 08:04:25: 31000000 INFO @ Sat, 11 Dec 2021 08:04:26: 27000000 INFO @ Sat, 11 Dec 2021 08:04:36: 34000000 INFO @ Sat, 11 Dec 2021 08:04:36: 32000000 INFO @ Sat, 11 Dec 2021 08:04:38: 28000000 INFO @ Sat, 11 Dec 2021 08:04:47: 35000000 INFO @ Sat, 11 Dec 2021 08:04:48: 33000000 INFO @ Sat, 11 Dec 2021 08:04:50: 29000000 INFO @ Sat, 11 Dec 2021 08:04:59: 34000000 INFO @ Sat, 11 Dec 2021 08:04:59: 36000000 INFO @ Sat, 11 Dec 2021 08:05:02: 30000000 INFO @ Sat, 11 Dec 2021 08:05:10: 35000000 INFO @ Sat, 11 Dec 2021 08:05:11: 37000000 INFO @ Sat, 11 Dec 2021 08:05:14: 31000000 INFO @ Sat, 11 Dec 2021 08:05:22: 36000000 INFO @ Sat, 11 Dec 2021 08:05:23: 38000000 INFO @ Sat, 11 Dec 2021 08:05:26: 32000000 INFO @ Sat, 11 Dec 2021 08:05:33: 37000000 INFO @ Sat, 11 Dec 2021 08:05:34: 39000000 INFO @ Sat, 11 Dec 2021 08:05:37: 33000000 INFO @ Sat, 11 Dec 2021 08:05:44: 38000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 08:05:46: 40000000 INFO @ Sat, 11 Dec 2021 08:05:49: 34000000 INFO @ Sat, 11 Dec 2021 08:05:56: 39000000 INFO @ Sat, 11 Dec 2021 08:05:58: 41000000 INFO @ Sat, 11 Dec 2021 08:06:01: 35000000 INFO @ Sat, 11 Dec 2021 08:06:07: 40000000 INFO @ Sat, 11 Dec 2021 08:06:10: 42000000 INFO @ Sat, 11 Dec 2021 08:06:13: 36000000 INFO @ Sat, 11 Dec 2021 08:06:18: 41000000 INFO @ Sat, 11 Dec 2021 08:06:21: 43000000 INFO @ Sat, 11 Dec 2021 08:06:25: 37000000 INFO @ Sat, 11 Dec 2021 08:06:29: 42000000 INFO @ Sat, 11 Dec 2021 08:06:33: 44000000 INFO @ Sat, 11 Dec 2021 08:06:37: 38000000 INFO @ Sat, 11 Dec 2021 08:06:40: 43000000 INFO @ Sat, 11 Dec 2021 08:06:44: 45000000 INFO @ Sat, 11 Dec 2021 08:06:49: 39000000 INFO @ Sat, 11 Dec 2021 08:06:51: 44000000 INFO @ Sat, 11 Dec 2021 08:06:55: 46000000 INFO @ Sat, 11 Dec 2021 08:07:01: 40000000 INFO @ Sat, 11 Dec 2021 08:07:02: 45000000 INFO @ Sat, 11 Dec 2021 08:07:07: 47000000 INFO @ Sat, 11 Dec 2021 08:07:12: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 08:07:12: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 08:07:12: #1 total tags in treatment: 47405506 INFO @ Sat, 11 Dec 2021 08:07:12: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:07:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:07:12: 41000000 INFO @ Sat, 11 Dec 2021 08:07:13: #1 tags after filtering in treatment: 47405506 INFO @ Sat, 11 Dec 2021 08:07:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:07:13: #1 finished! INFO @ Sat, 11 Dec 2021 08:07:13: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:07:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:07:13: 46000000 INFO @ Sat, 11 Dec 2021 08:07:16: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 08:07:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 08:07:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:07:24: 47000000 INFO @ Sat, 11 Dec 2021 08:07:24: 42000000 INFO @ Sat, 11 Dec 2021 08:07:29: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 08:07:29: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 08:07:29: #1 total tags in treatment: 47405506 INFO @ Sat, 11 Dec 2021 08:07:29: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:07:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:07:30: #1 tags after filtering in treatment: 47405506 INFO @ Sat, 11 Dec 2021 08:07:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:07:30: #1 finished! INFO @ Sat, 11 Dec 2021 08:07:30: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:07:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:07:33: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 08:07:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 08:07:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 08:07:36: 43000000 INFO @ Sat, 11 Dec 2021 08:07:47: 44000000 INFO @ Sat, 11 Dec 2021 08:07:58: 45000000 INFO @ Sat, 11 Dec 2021 08:08:10: 46000000 INFO @ Sat, 11 Dec 2021 08:08:21: 47000000 INFO @ Sat, 11 Dec 2021 08:08:26: #1 tag size is determined as 75 bps INFO @ Sat, 11 Dec 2021 08:08:26: #1 tag size = 75 INFO @ Sat, 11 Dec 2021 08:08:26: #1 total tags in treatment: 47405506 INFO @ Sat, 11 Dec 2021 08:08:26: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 08:08:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 08:08:27: #1 tags after filtering in treatment: 47405506 INFO @ Sat, 11 Dec 2021 08:08:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 08:08:27: #1 finished! INFO @ Sat, 11 Dec 2021 08:08:27: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 08:08:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 08:08:31: #2 number of paired peaks: 0 WARNING @ Sat, 11 Dec 2021 08:08:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 08:08:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8174954/SRX8174954.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling