Job ID = 6627008 SRX = SRX8156261 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T00:19:18 prefetch.2.10.7: 1) Downloading 'SRR11588806'... 2020-07-14T00:19:18 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T00:20:22 prefetch.2.10.7: HTTPS download succeed 2020-07-14T00:20:22 prefetch.2.10.7: 1) 'SRR11588806' was downloaded successfully Read 25070454 spots for SRR11588806/SRR11588806.sra Written 25070454 spots for SRR11588806/SRR11588806.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627159 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:48 25070454 reads; of these: 25070454 (100.00%) were unpaired; of these: 2020562 (8.06%) aligned 0 times 17661934 (70.45%) aligned exactly 1 time 5387958 (21.49%) aligned >1 times 91.94% overall alignment rate Time searching: 00:06:49 Overall time: 00:06:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4775590 / 23049892 = 0.2072 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:32:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:32:57: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:32:57: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:33:03: 1000000 INFO @ Tue, 14 Jul 2020 09:33:10: 2000000 INFO @ Tue, 14 Jul 2020 09:33:16: 3000000 INFO @ Tue, 14 Jul 2020 09:33:23: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:33:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:33:26: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:33:26: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:33:30: 5000000 INFO @ Tue, 14 Jul 2020 09:33:33: 1000000 INFO @ Tue, 14 Jul 2020 09:33:37: 6000000 INFO @ Tue, 14 Jul 2020 09:33:40: 2000000 INFO @ Tue, 14 Jul 2020 09:33:44: 7000000 INFO @ Tue, 14 Jul 2020 09:33:46: 3000000 INFO @ Tue, 14 Jul 2020 09:33:51: 8000000 INFO @ Tue, 14 Jul 2020 09:33:53: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:33:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:33:56: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:33:56: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:33:58: 9000000 INFO @ Tue, 14 Jul 2020 09:33:59: 5000000 INFO @ Tue, 14 Jul 2020 09:34:04: 1000000 INFO @ Tue, 14 Jul 2020 09:34:05: 10000000 INFO @ Tue, 14 Jul 2020 09:34:06: 6000000 INFO @ Tue, 14 Jul 2020 09:34:11: 2000000 INFO @ Tue, 14 Jul 2020 09:34:12: 11000000 INFO @ Tue, 14 Jul 2020 09:34:12: 7000000 INFO @ Tue, 14 Jul 2020 09:34:18: 3000000 INFO @ Tue, 14 Jul 2020 09:34:19: 8000000 INFO @ Tue, 14 Jul 2020 09:34:19: 12000000 INFO @ Tue, 14 Jul 2020 09:34:26: 9000000 INFO @ Tue, 14 Jul 2020 09:34:26: 4000000 INFO @ Tue, 14 Jul 2020 09:34:27: 13000000 INFO @ Tue, 14 Jul 2020 09:34:32: 10000000 INFO @ Tue, 14 Jul 2020 09:34:33: 5000000 INFO @ Tue, 14 Jul 2020 09:34:34: 14000000 INFO @ Tue, 14 Jul 2020 09:34:39: 11000000 INFO @ Tue, 14 Jul 2020 09:34:40: 6000000 INFO @ Tue, 14 Jul 2020 09:34:41: 15000000 INFO @ Tue, 14 Jul 2020 09:34:45: 12000000 INFO @ Tue, 14 Jul 2020 09:34:47: 7000000 INFO @ Tue, 14 Jul 2020 09:34:48: 16000000 INFO @ Tue, 14 Jul 2020 09:34:52: 13000000 INFO @ Tue, 14 Jul 2020 09:34:54: 8000000 INFO @ Tue, 14 Jul 2020 09:34:56: 17000000 INFO @ Tue, 14 Jul 2020 09:34:59: 14000000 INFO @ Tue, 14 Jul 2020 09:35:01: 9000000 INFO @ Tue, 14 Jul 2020 09:35:03: 18000000 INFO @ Tue, 14 Jul 2020 09:35:05: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:35:05: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:35:05: #1 total tags in treatment: 18274302 INFO @ Tue, 14 Jul 2020 09:35:05: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:35:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:35:05: #1 tags after filtering in treatment: 18274302 INFO @ Tue, 14 Jul 2020 09:35:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:35:05: #1 finished! INFO @ Tue, 14 Jul 2020 09:35:05: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:35:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:35:05: 15000000 INFO @ Tue, 14 Jul 2020 09:35:06: #2 number of paired peaks: 8 WARNING @ Tue, 14 Jul 2020 09:35:06: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:35:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:35:08: 10000000 INFO @ Tue, 14 Jul 2020 09:35:12: 16000000 INFO @ Tue, 14 Jul 2020 09:35:15: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:35:18: 17000000 INFO @ Tue, 14 Jul 2020 09:35:22: 12000000 INFO @ Tue, 14 Jul 2020 09:35:24: 18000000 INFO @ Tue, 14 Jul 2020 09:35:26: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:35:26: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:35:26: #1 total tags in treatment: 18274302 INFO @ Tue, 14 Jul 2020 09:35:26: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:35:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:35:26: #1 tags after filtering in treatment: 18274302 INFO @ Tue, 14 Jul 2020 09:35:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:35:26: #1 finished! INFO @ Tue, 14 Jul 2020 09:35:26: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:35:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:35:28: #2 number of paired peaks: 8 WARNING @ Tue, 14 Jul 2020 09:35:28: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:35:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:35:29: 13000000 INFO @ Tue, 14 Jul 2020 09:35:36: 14000000 INFO @ Tue, 14 Jul 2020 09:35:43: 15000000 INFO @ Tue, 14 Jul 2020 09:35:49: 16000000 INFO @ Tue, 14 Jul 2020 09:35:56: 17000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 09:36:02: 18000000 INFO @ Tue, 14 Jul 2020 09:36:04: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:36:04: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:36:04: #1 total tags in treatment: 18274302 INFO @ Tue, 14 Jul 2020 09:36:04: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:36:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:36:04: #1 tags after filtering in treatment: 18274302 INFO @ Tue, 14 Jul 2020 09:36:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:36:04: #1 finished! INFO @ Tue, 14 Jul 2020 09:36:04: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:36:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:36:05: #2 number of paired peaks: 8 WARNING @ Tue, 14 Jul 2020 09:36:05: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:36:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX8156261/SRX8156261.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling