Job ID = 6528509 SRX = SRX815529 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:56:43 prefetch.2.10.7: 1) Downloading 'SRR1721317'... 2020-06-29T14:56:43 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:01:51 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:01:51 prefetch.2.10.7: 1) 'SRR1721317' was downloaded successfully Read 32988934 spots for SRR1721317/SRR1721317.sra Written 32988934 spots for SRR1721317/SRR1721317.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:51 32988934 reads; of these: 32988934 (100.00%) were unpaired; of these: 2579172 (7.82%) aligned 0 times 23729383 (71.93%) aligned exactly 1 time 6680379 (20.25%) aligned >1 times 92.18% overall alignment rate Time searching: 00:08:52 Overall time: 00:08:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6186849 / 30409762 = 0.2034 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:27:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:27:26: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:27:26: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:27:32: 1000000 INFO @ Tue, 30 Jun 2020 00:27:37: 2000000 INFO @ Tue, 30 Jun 2020 00:27:43: 3000000 INFO @ Tue, 30 Jun 2020 00:27:48: 4000000 INFO @ Tue, 30 Jun 2020 00:27:54: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:27:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:27:56: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:27:56: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:28:00: 6000000 INFO @ Tue, 30 Jun 2020 00:28:01: 1000000 INFO @ Tue, 30 Jun 2020 00:28:05: 7000000 INFO @ Tue, 30 Jun 2020 00:28:07: 2000000 INFO @ Tue, 30 Jun 2020 00:28:11: 8000000 INFO @ Tue, 30 Jun 2020 00:28:12: 3000000 INFO @ Tue, 30 Jun 2020 00:28:16: 9000000 INFO @ Tue, 30 Jun 2020 00:28:18: 4000000 INFO @ Tue, 30 Jun 2020 00:28:22: 10000000 INFO @ Tue, 30 Jun 2020 00:28:23: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:28:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:28:26: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:28:26: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:28:27: 11000000 INFO @ Tue, 30 Jun 2020 00:28:29: 6000000 INFO @ Tue, 30 Jun 2020 00:28:31: 1000000 INFO @ Tue, 30 Jun 2020 00:28:33: 12000000 INFO @ Tue, 30 Jun 2020 00:28:35: 7000000 INFO @ Tue, 30 Jun 2020 00:28:37: 2000000 INFO @ Tue, 30 Jun 2020 00:28:38: 13000000 INFO @ Tue, 30 Jun 2020 00:28:40: 8000000 INFO @ Tue, 30 Jun 2020 00:28:42: 3000000 INFO @ Tue, 30 Jun 2020 00:28:43: 14000000 INFO @ Tue, 30 Jun 2020 00:28:45: 9000000 INFO @ Tue, 30 Jun 2020 00:28:47: 4000000 INFO @ Tue, 30 Jun 2020 00:28:49: 15000000 INFO @ Tue, 30 Jun 2020 00:28:51: 10000000 INFO @ Tue, 30 Jun 2020 00:28:52: 5000000 INFO @ Tue, 30 Jun 2020 00:28:54: 16000000 INFO @ Tue, 30 Jun 2020 00:28:56: 11000000 INFO @ Tue, 30 Jun 2020 00:28:57: 6000000 INFO @ Tue, 30 Jun 2020 00:29:00: 17000000 INFO @ Tue, 30 Jun 2020 00:29:02: 12000000 INFO @ Tue, 30 Jun 2020 00:29:02: 7000000 INFO @ Tue, 30 Jun 2020 00:29:05: 18000000 INFO @ Tue, 30 Jun 2020 00:29:07: 13000000 INFO @ Tue, 30 Jun 2020 00:29:07: 8000000 INFO @ Tue, 30 Jun 2020 00:29:11: 19000000 INFO @ Tue, 30 Jun 2020 00:29:12: 9000000 INFO @ Tue, 30 Jun 2020 00:29:12: 14000000 INFO @ Tue, 30 Jun 2020 00:29:16: 20000000 INFO @ Tue, 30 Jun 2020 00:29:17: 10000000 INFO @ Tue, 30 Jun 2020 00:29:18: 15000000 INFO @ Tue, 30 Jun 2020 00:29:22: 21000000 INFO @ Tue, 30 Jun 2020 00:29:22: 11000000 INFO @ Tue, 30 Jun 2020 00:29:23: 16000000 INFO @ Tue, 30 Jun 2020 00:29:27: 12000000 INFO @ Tue, 30 Jun 2020 00:29:27: 22000000 INFO @ Tue, 30 Jun 2020 00:29:28: 17000000 INFO @ Tue, 30 Jun 2020 00:29:32: 13000000 INFO @ Tue, 30 Jun 2020 00:29:33: 23000000 INFO @ Tue, 30 Jun 2020 00:29:34: 18000000 INFO @ Tue, 30 Jun 2020 00:29:37: 14000000 INFO @ Tue, 30 Jun 2020 00:29:38: 24000000 INFO @ Tue, 30 Jun 2020 00:29:39: 19000000 INFO @ Tue, 30 Jun 2020 00:29:40: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:29:40: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:29:40: #1 total tags in treatment: 24222913 INFO @ Tue, 30 Jun 2020 00:29:40: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:29:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:29:40: #1 tags after filtering in treatment: 24222913 INFO @ Tue, 30 Jun 2020 00:29:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:29:40: #1 finished! INFO @ Tue, 30 Jun 2020 00:29:40: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:29:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:29:42: #2 number of paired peaks: 59 WARNING @ Tue, 30 Jun 2020 00:29:42: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:29:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:29:42: 15000000 INFO @ Tue, 30 Jun 2020 00:29:45: 20000000 INFO @ Tue, 30 Jun 2020 00:29:47: 16000000 INFO @ Tue, 30 Jun 2020 00:29:50: 21000000 INFO @ Tue, 30 Jun 2020 00:29:52: 17000000 INFO @ Tue, 30 Jun 2020 00:29:56: 22000000 INFO @ Tue, 30 Jun 2020 00:29:57: 18000000 INFO @ Tue, 30 Jun 2020 00:30:01: 23000000 INFO @ Tue, 30 Jun 2020 00:30:02: 19000000 INFO @ Tue, 30 Jun 2020 00:30:07: 24000000 INFO @ Tue, 30 Jun 2020 00:30:07: 20000000 INFO @ Tue, 30 Jun 2020 00:30:08: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:30:08: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:30:08: #1 total tags in treatment: 24222913 INFO @ Tue, 30 Jun 2020 00:30:08: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:30:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:30:09: #1 tags after filtering in treatment: 24222913 INFO @ Tue, 30 Jun 2020 00:30:09: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:30:09: #1 finished! INFO @ Tue, 30 Jun 2020 00:30:09: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:30:09: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:30:10: #2 number of paired peaks: 59 WARNING @ Tue, 30 Jun 2020 00:30:10: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:30:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:30:12: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:30:17: 22000000 INFO @ Tue, 30 Jun 2020 00:30:22: 23000000 INFO @ Tue, 30 Jun 2020 00:30:27: 24000000 INFO @ Tue, 30 Jun 2020 00:30:28: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:30:28: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:30:28: #1 total tags in treatment: 24222913 INFO @ Tue, 30 Jun 2020 00:30:28: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:30:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:30:29: #1 tags after filtering in treatment: 24222913 INFO @ Tue, 30 Jun 2020 00:30:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:30:29: #1 finished! INFO @ Tue, 30 Jun 2020 00:30:29: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:30:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:30:30: #2 number of paired peaks: 59 WARNING @ Tue, 30 Jun 2020 00:30:30: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:30:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX815529/SRX815529.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。