Job ID = 6626995 SRX = SRX7988218 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 32593705 spots for SRR11409516/SRR11409516.sra Written 32593705 spots for SRR11409516/SRR11409516.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627398 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:14:54 32593705 reads; of these: 32593705 (100.00%) were paired; of these: 5974539 (18.33%) aligned concordantly 0 times 18886546 (57.95%) aligned concordantly exactly 1 time 7732620 (23.72%) aligned concordantly >1 times ---- 5974539 pairs aligned concordantly 0 times; of these: 2562302 (42.89%) aligned discordantly 1 time ---- 3412237 pairs aligned 0 times concordantly or discordantly; of these: 6824474 mates make up the pairs; of these: 3589607 (52.60%) aligned 0 times 1235531 (18.10%) aligned exactly 1 time 1999336 (29.30%) aligned >1 times 94.49% overall alignment rate Time searching: 01:14:54 Overall time: 01:14:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2533336 / 29100327 = 0.0871 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:48:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:48:34: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:48:34: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:48:41: 1000000 INFO @ Tue, 14 Jul 2020 10:48:48: 2000000 INFO @ Tue, 14 Jul 2020 10:48:55: 3000000 INFO @ Tue, 14 Jul 2020 10:49:02: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:49:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:49:04: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:49:04: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:49:09: 5000000 INFO @ Tue, 14 Jul 2020 10:49:13: 1000000 INFO @ Tue, 14 Jul 2020 10:49:17: 6000000 INFO @ Tue, 14 Jul 2020 10:49:20: 2000000 INFO @ Tue, 14 Jul 2020 10:49:25: 7000000 INFO @ Tue, 14 Jul 2020 10:49:28: 3000000 INFO @ Tue, 14 Jul 2020 10:49:32: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 10:49:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 10:49:34: #1 read tag files... INFO @ Tue, 14 Jul 2020 10:49:34: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 10:49:36: 4000000 INFO @ Tue, 14 Jul 2020 10:49:40: 9000000 INFO @ Tue, 14 Jul 2020 10:49:42: 1000000 INFO @ Tue, 14 Jul 2020 10:49:44: 5000000 INFO @ Tue, 14 Jul 2020 10:49:48: 10000000 INFO @ Tue, 14 Jul 2020 10:49:50: 2000000 INFO @ Tue, 14 Jul 2020 10:49:51: 6000000 INFO @ Tue, 14 Jul 2020 10:49:55: 11000000 INFO @ Tue, 14 Jul 2020 10:49:58: 3000000 INFO @ Tue, 14 Jul 2020 10:49:59: 7000000 INFO @ Tue, 14 Jul 2020 10:50:03: 12000000 INFO @ Tue, 14 Jul 2020 10:50:05: 4000000 INFO @ Tue, 14 Jul 2020 10:50:07: 8000000 INFO @ Tue, 14 Jul 2020 10:50:11: 13000000 INFO @ Tue, 14 Jul 2020 10:50:13: 5000000 INFO @ Tue, 14 Jul 2020 10:50:15: 9000000 INFO @ Tue, 14 Jul 2020 10:50:18: 14000000 INFO @ Tue, 14 Jul 2020 10:50:21: 6000000 INFO @ Tue, 14 Jul 2020 10:50:22: 10000000 INFO @ Tue, 14 Jul 2020 10:50:26: 15000000 INFO @ Tue, 14 Jul 2020 10:50:28: 7000000 INFO @ Tue, 14 Jul 2020 10:50:30: 11000000 INFO @ Tue, 14 Jul 2020 10:50:34: 16000000 INFO @ Tue, 14 Jul 2020 10:50:36: 8000000 INFO @ Tue, 14 Jul 2020 10:50:37: 12000000 INFO @ Tue, 14 Jul 2020 10:50:41: 17000000 INFO @ Tue, 14 Jul 2020 10:50:43: 9000000 INFO @ Tue, 14 Jul 2020 10:50:45: 13000000 INFO @ Tue, 14 Jul 2020 10:50:49: 18000000 INFO @ Tue, 14 Jul 2020 10:50:51: 10000000 INFO @ Tue, 14 Jul 2020 10:50:53: 14000000 INFO @ Tue, 14 Jul 2020 10:50:56: 19000000 INFO @ Tue, 14 Jul 2020 10:50:58: 11000000 INFO @ Tue, 14 Jul 2020 10:51:00: 15000000 INFO @ Tue, 14 Jul 2020 10:51:04: 20000000 INFO @ Tue, 14 Jul 2020 10:51:06: 12000000 INFO @ Tue, 14 Jul 2020 10:51:08: 16000000 INFO @ Tue, 14 Jul 2020 10:51:11: 21000000 INFO @ Tue, 14 Jul 2020 10:51:13: 13000000 INFO @ Tue, 14 Jul 2020 10:51:15: 17000000 INFO @ Tue, 14 Jul 2020 10:51:18: 22000000 INFO @ Tue, 14 Jul 2020 10:51:21: 14000000 INFO @ Tue, 14 Jul 2020 10:51:22: 18000000 INFO @ Tue, 14 Jul 2020 10:51:26: 23000000 INFO @ Tue, 14 Jul 2020 10:51:28: 15000000 INFO @ Tue, 14 Jul 2020 10:51:30: 19000000 INFO @ Tue, 14 Jul 2020 10:51:33: 24000000 INFO @ Tue, 14 Jul 2020 10:51:35: 16000000 INFO @ Tue, 14 Jul 2020 10:51:37: 20000000 INFO @ Tue, 14 Jul 2020 10:51:40: 25000000 INFO @ Tue, 14 Jul 2020 10:51:43: 17000000 INFO @ Tue, 14 Jul 2020 10:51:45: 21000000 INFO @ Tue, 14 Jul 2020 10:51:48: 26000000 INFO @ Tue, 14 Jul 2020 10:51:50: 18000000 INFO @ Tue, 14 Jul 2020 10:51:52: 22000000 INFO @ Tue, 14 Jul 2020 10:51:55: 27000000 INFO @ Tue, 14 Jul 2020 10:51:57: 19000000 INFO @ Tue, 14 Jul 2020 10:51:59: 23000000 INFO @ Tue, 14 Jul 2020 10:52:02: 28000000 INFO @ Tue, 14 Jul 2020 10:52:05: 20000000 INFO @ Tue, 14 Jul 2020 10:52:07: 24000000 INFO @ Tue, 14 Jul 2020 10:52:09: 29000000 INFO @ Tue, 14 Jul 2020 10:52:12: 21000000 INFO @ Tue, 14 Jul 2020 10:52:14: 25000000 INFO @ Tue, 14 Jul 2020 10:52:17: 30000000 INFO @ Tue, 14 Jul 2020 10:52:19: 22000000 INFO @ Tue, 14 Jul 2020 10:52:21: 26000000 INFO @ Tue, 14 Jul 2020 10:52:24: 31000000 INFO @ Tue, 14 Jul 2020 10:52:26: 23000000 INFO @ Tue, 14 Jul 2020 10:52:29: 27000000 INFO @ Tue, 14 Jul 2020 10:52:31: 32000000 INFO @ Tue, 14 Jul 2020 10:52:34: 24000000 INFO @ Tue, 14 Jul 2020 10:52:36: 28000000 INFO @ Tue, 14 Jul 2020 10:52:38: 33000000 INFO @ Tue, 14 Jul 2020 10:52:41: 25000000 INFO @ Tue, 14 Jul 2020 10:52:43: 29000000 INFO @ Tue, 14 Jul 2020 10:52:46: 34000000 INFO @ Tue, 14 Jul 2020 10:52:48: 26000000 INFO @ Tue, 14 Jul 2020 10:52:51: 30000000 INFO @ Tue, 14 Jul 2020 10:52:53: 35000000 INFO @ Tue, 14 Jul 2020 10:52:56: 27000000 INFO @ Tue, 14 Jul 2020 10:52:58: 31000000 INFO @ Tue, 14 Jul 2020 10:53:00: 36000000 INFO @ Tue, 14 Jul 2020 10:53:03: 28000000 INFO @ Tue, 14 Jul 2020 10:53:05: 32000000 INFO @ Tue, 14 Jul 2020 10:53:08: 37000000 INFO @ Tue, 14 Jul 2020 10:53:10: 29000000 INFO @ Tue, 14 Jul 2020 10:53:13: 33000000 INFO @ Tue, 14 Jul 2020 10:53:15: 38000000 INFO @ Tue, 14 Jul 2020 10:53:17: 30000000 INFO @ Tue, 14 Jul 2020 10:53:20: 34000000 INFO @ Tue, 14 Jul 2020 10:53:23: 39000000 INFO @ Tue, 14 Jul 2020 10:53:25: 31000000 INFO @ Tue, 14 Jul 2020 10:53:27: 35000000 INFO @ Tue, 14 Jul 2020 10:53:30: 40000000 INFO @ Tue, 14 Jul 2020 10:53:32: 32000000 INFO @ Tue, 14 Jul 2020 10:53:35: 36000000 INFO @ Tue, 14 Jul 2020 10:53:38: 41000000 INFO @ Tue, 14 Jul 2020 10:53:39: 33000000 INFO @ Tue, 14 Jul 2020 10:53:42: 37000000 INFO @ Tue, 14 Jul 2020 10:53:45: 42000000 INFO @ Tue, 14 Jul 2020 10:53:46: 34000000 INFO @ Tue, 14 Jul 2020 10:53:49: 38000000 INFO @ Tue, 14 Jul 2020 10:53:52: 43000000 INFO @ Tue, 14 Jul 2020 10:53:54: 35000000 INFO @ Tue, 14 Jul 2020 10:53:57: 39000000 INFO @ Tue, 14 Jul 2020 10:54:00: 44000000 INFO @ Tue, 14 Jul 2020 10:54:01: 36000000 INFO @ Tue, 14 Jul 2020 10:54:05: 40000000 INFO @ Tue, 14 Jul 2020 10:54:07: 45000000 INFO @ Tue, 14 Jul 2020 10:54:08: 37000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 10:54:12: 41000000 INFO @ Tue, 14 Jul 2020 10:54:14: 46000000 INFO @ Tue, 14 Jul 2020 10:54:16: 38000000 INFO @ Tue, 14 Jul 2020 10:54:19: 42000000 INFO @ Tue, 14 Jul 2020 10:54:21: 47000000 INFO @ Tue, 14 Jul 2020 10:54:24: 39000000 INFO @ Tue, 14 Jul 2020 10:54:27: 43000000 INFO @ Tue, 14 Jul 2020 10:54:29: 48000000 INFO @ Tue, 14 Jul 2020 10:54:31: 40000000 INFO @ Tue, 14 Jul 2020 10:54:34: 44000000 INFO @ Tue, 14 Jul 2020 10:54:36: 49000000 INFO @ Tue, 14 Jul 2020 10:54:38: 41000000 INFO @ Tue, 14 Jul 2020 10:54:41: 45000000 INFO @ Tue, 14 Jul 2020 10:54:43: 50000000 INFO @ Tue, 14 Jul 2020 10:54:45: 42000000 INFO @ Tue, 14 Jul 2020 10:54:49: 46000000 INFO @ Tue, 14 Jul 2020 10:54:51: 51000000 INFO @ Tue, 14 Jul 2020 10:54:53: 43000000 INFO @ Tue, 14 Jul 2020 10:54:56: 47000000 INFO @ Tue, 14 Jul 2020 10:54:58: 52000000 INFO @ Tue, 14 Jul 2020 10:55:00: 44000000 INFO @ Tue, 14 Jul 2020 10:55:04: 48000000 INFO @ Tue, 14 Jul 2020 10:55:05: 53000000 INFO @ Tue, 14 Jul 2020 10:55:07: 45000000 INFO @ Tue, 14 Jul 2020 10:55:11: 49000000 INFO @ Tue, 14 Jul 2020 10:55:13: 54000000 INFO @ Tue, 14 Jul 2020 10:55:14: 46000000 INFO @ Tue, 14 Jul 2020 10:55:19: 50000000 INFO @ Tue, 14 Jul 2020 10:55:20: 55000000 INFO @ Tue, 14 Jul 2020 10:55:22: 47000000 INFO @ Tue, 14 Jul 2020 10:55:26: 51000000 INFO @ Tue, 14 Jul 2020 10:55:27: 56000000 INFO @ Tue, 14 Jul 2020 10:55:29: 48000000 INFO @ Tue, 14 Jul 2020 10:55:32: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 10:55:32: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 10:55:32: #1 total tags in treatment: 24334947 INFO @ Tue, 14 Jul 2020 10:55:32: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:55:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:55:32: #1 tags after filtering in treatment: 20456106 INFO @ Tue, 14 Jul 2020 10:55:32: #1 Redundant rate of treatment: 0.16 INFO @ Tue, 14 Jul 2020 10:55:32: #1 finished! INFO @ Tue, 14 Jul 2020 10:55:32: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:55:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:55:33: #2 number of paired peaks: 18 WARNING @ Tue, 14 Jul 2020 10:55:33: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:55:33: Process for pairing-model is terminated! INFO @ Tue, 14 Jul 2020 10:55:33: 52000000 cut: /home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:55:36: 49000000 INFO @ Tue, 14 Jul 2020 10:55:41: 53000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 10:55:44: 50000000 INFO @ Tue, 14 Jul 2020 10:55:48: 54000000 INFO @ Tue, 14 Jul 2020 10:55:51: 51000000 INFO @ Tue, 14 Jul 2020 10:55:56: 55000000 INFO @ Tue, 14 Jul 2020 10:55:58: 52000000 INFO @ Tue, 14 Jul 2020 10:56:03: 56000000 INFO @ Tue, 14 Jul 2020 10:56:06: 53000000 INFO @ Tue, 14 Jul 2020 10:56:07: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 10:56:07: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 10:56:07: #1 total tags in treatment: 24334947 INFO @ Tue, 14 Jul 2020 10:56:07: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:56:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:56:08: #1 tags after filtering in treatment: 20456106 INFO @ Tue, 14 Jul 2020 10:56:08: #1 Redundant rate of treatment: 0.16 INFO @ Tue, 14 Jul 2020 10:56:08: #1 finished! INFO @ Tue, 14 Jul 2020 10:56:08: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:56:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:56:09: #2 number of paired peaks: 18 WARNING @ Tue, 14 Jul 2020 10:56:09: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:56:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 10:56:13: 54000000 INFO @ Tue, 14 Jul 2020 10:56:20: 55000000 INFO @ Tue, 14 Jul 2020 10:56:26: 56000000 INFO @ Tue, 14 Jul 2020 10:56:30: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 10:56:30: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 10:56:30: #1 total tags in treatment: 24334947 INFO @ Tue, 14 Jul 2020 10:56:30: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 10:56:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 10:56:30: #1 tags after filtering in treatment: 20456106 INFO @ Tue, 14 Jul 2020 10:56:30: #1 Redundant rate of treatment: 0.16 INFO @ Tue, 14 Jul 2020 10:56:30: #1 finished! INFO @ Tue, 14 Jul 2020 10:56:30: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 10:56:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 10:56:32: #2 number of paired peaks: 18 WARNING @ Tue, 14 Jul 2020 10:56:32: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 10:56:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7988218/SRX7988218.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling