Job ID = 6626989 SRX = SRX7975013 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T00:10:46 prefetch.2.10.7: 1) Downloading 'SRR11396266'... 2020-07-14T00:10:46 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T00:14:51 prefetch.2.10.7: HTTPS download succeed 2020-07-14T00:14:51 prefetch.2.10.7: 1) 'SRR11396266' was downloaded successfully Read 27270369 spots for SRR11396266/SRR11396266.sra Written 27270369 spots for SRR11396266/SRR11396266.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627147 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:52 27270369 reads; of these: 27270369 (100.00%) were unpaired; of these: 6174007 (22.64%) aligned 0 times 15467633 (56.72%) aligned exactly 1 time 5628729 (20.64%) aligned >1 times 77.36% overall alignment rate Time searching: 00:07:53 Overall time: 00:07:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4840648 / 21096362 = 0.2295 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:29:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:29:19: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:29:19: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:29:24: 1000000 INFO @ Tue, 14 Jul 2020 09:29:29: 2000000 INFO @ Tue, 14 Jul 2020 09:29:34: 3000000 INFO @ Tue, 14 Jul 2020 09:29:40: 4000000 INFO @ Tue, 14 Jul 2020 09:29:45: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:29:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:29:49: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:29:49: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:29:50: 6000000 INFO @ Tue, 14 Jul 2020 09:29:54: 1000000 INFO @ Tue, 14 Jul 2020 09:29:55: 7000000 INFO @ Tue, 14 Jul 2020 09:29:59: 2000000 INFO @ Tue, 14 Jul 2020 09:30:00: 8000000 INFO @ Tue, 14 Jul 2020 09:30:04: 3000000 INFO @ Tue, 14 Jul 2020 09:30:06: 9000000 INFO @ Tue, 14 Jul 2020 09:30:09: 4000000 INFO @ Tue, 14 Jul 2020 09:30:11: 10000000 INFO @ Tue, 14 Jul 2020 09:30:15: 5000000 INFO @ Tue, 14 Jul 2020 09:30:16: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:30:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:30:19: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:30:19: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:30:20: 6000000 INFO @ Tue, 14 Jul 2020 09:30:21: 12000000 INFO @ Tue, 14 Jul 2020 09:30:24: 1000000 INFO @ Tue, 14 Jul 2020 09:30:25: 7000000 INFO @ Tue, 14 Jul 2020 09:30:26: 13000000 INFO @ Tue, 14 Jul 2020 09:30:29: 2000000 INFO @ Tue, 14 Jul 2020 09:30:30: 8000000 INFO @ Tue, 14 Jul 2020 09:30:31: 14000000 INFO @ Tue, 14 Jul 2020 09:30:35: 3000000 INFO @ Tue, 14 Jul 2020 09:30:36: 9000000 INFO @ Tue, 14 Jul 2020 09:30:36: 15000000 INFO @ Tue, 14 Jul 2020 09:30:40: 4000000 INFO @ Tue, 14 Jul 2020 09:30:41: 10000000 INFO @ Tue, 14 Jul 2020 09:30:41: 16000000 INFO @ Tue, 14 Jul 2020 09:30:43: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:30:43: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:30:43: #1 total tags in treatment: 16255714 INFO @ Tue, 14 Jul 2020 09:30:43: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:30:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:30:43: #1 tags after filtering in treatment: 16255714 INFO @ Tue, 14 Jul 2020 09:30:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:30:43: #1 finished! INFO @ Tue, 14 Jul 2020 09:30:43: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:30:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:30:44: #2 number of paired peaks: 61 WARNING @ Tue, 14 Jul 2020 09:30:44: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:30:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:30:46: 11000000 INFO @ Tue, 14 Jul 2020 09:30:46: 5000000 INFO @ Tue, 14 Jul 2020 09:30:51: 12000000 INFO @ Tue, 14 Jul 2020 09:30:52: 6000000 INFO @ Tue, 14 Jul 2020 09:30:56: 13000000 INFO @ Tue, 14 Jul 2020 09:30:57: 7000000 INFO @ Tue, 14 Jul 2020 09:31:01: 14000000 INFO @ Tue, 14 Jul 2020 09:31:02: 8000000 INFO @ Tue, 14 Jul 2020 09:31:06: 15000000 INFO @ Tue, 14 Jul 2020 09:31:08: 9000000 INFO @ Tue, 14 Jul 2020 09:31:11: 16000000 INFO @ Tue, 14 Jul 2020 09:31:13: 10000000 INFO @ Tue, 14 Jul 2020 09:31:13: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:31:13: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:31:13: #1 total tags in treatment: 16255714 INFO @ Tue, 14 Jul 2020 09:31:13: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:31:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:31:13: #1 tags after filtering in treatment: 16255714 INFO @ Tue, 14 Jul 2020 09:31:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:31:13: #1 finished! INFO @ Tue, 14 Jul 2020 09:31:13: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:31:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:31:14: #2 number of paired peaks: 61 WARNING @ Tue, 14 Jul 2020 09:31:14: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:31:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:31:18: 11000000 INFO @ Tue, 14 Jul 2020 09:31:24: 12000000 INFO @ Tue, 14 Jul 2020 09:31:29: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:31:34: 14000000 INFO @ Tue, 14 Jul 2020 09:31:39: 15000000 INFO @ Tue, 14 Jul 2020 09:31:44: 16000000 INFO @ Tue, 14 Jul 2020 09:31:45: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:31:45: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:31:45: #1 total tags in treatment: 16255714 INFO @ Tue, 14 Jul 2020 09:31:45: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:31:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:31:45: #1 tags after filtering in treatment: 16255714 INFO @ Tue, 14 Jul 2020 09:31:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:31:45: #1 finished! INFO @ Tue, 14 Jul 2020 09:31:45: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:31:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:31:46: #2 number of paired peaks: 61 WARNING @ Tue, 14 Jul 2020 09:31:46: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:31:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7975013/SRX7975013.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。