Job ID = 8069528 SRX = SRX7917372 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-08-08T03:34:14 prefetch.2.10.7: 1) Downloading 'SRR11312919'... 2020-08-08T03:34:14 prefetch.2.10.7: Downloading via HTTPS... 2020-08-08T03:34:36 prefetch.2.10.7: HTTPS download succeed 2020-08-08T03:34:37 prefetch.2.10.7: 'SRR11312919' is valid 2020-08-08T03:34:37 prefetch.2.10.7: 1) 'SRR11312919' was downloaded successfully Read 1205201 spots for SRR11312919/SRR11312919.sra Written 1205201 spots for SRR11312919/SRR11312919.sra fastq に変換しました。 bowtie でマッピング中... Your job 8069708 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:57 1205201 reads; of these: 1205201 (100.00%) were paired; of these: 301459 (25.01%) aligned concordantly 0 times 725963 (60.24%) aligned concordantly exactly 1 time 177779 (14.75%) aligned concordantly >1 times ---- 301459 pairs aligned concordantly 0 times; of these: 78796 (26.14%) aligned discordantly 1 time ---- 222663 pairs aligned 0 times concordantly or discordantly; of these: 445326 mates make up the pairs; of these: 328896 (73.86%) aligned 0 times 58326 (13.10%) aligned exactly 1 time 58104 (13.05%) aligned >1 times 86.36% overall alignment rate Time searching: 00:01:57 Overall time: 00:01:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 373227 / 978307 = 0.3815 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 12:37:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 12:37:41: #1 read tag files... INFO @ Sat, 08 Aug 2020 12:37:41: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 12:37:47: 1000000 INFO @ Sat, 08 Aug 2020 12:37:49: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 12:37:49: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 12:37:49: #1 total tags in treatment: 549245 INFO @ Sat, 08 Aug 2020 12:37:49: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 12:37:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 12:37:49: #1 tags after filtering in treatment: 469147 INFO @ Sat, 08 Aug 2020 12:37:49: #1 Redundant rate of treatment: 0.15 INFO @ Sat, 08 Aug 2020 12:37:49: #1 finished! INFO @ Sat, 08 Aug 2020 12:37:49: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 12:37:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 12:37:49: #2 number of paired peaks: 1762 INFO @ Sat, 08 Aug 2020 12:37:49: start model_add_line... INFO @ Sat, 08 Aug 2020 12:37:49: start X-correlation... INFO @ Sat, 08 Aug 2020 12:37:49: end of X-cor INFO @ Sat, 08 Aug 2020 12:37:49: #2 finished! INFO @ Sat, 08 Aug 2020 12:37:49: #2 predicted fragment length is 201 bps INFO @ Sat, 08 Aug 2020 12:37:49: #2 alternative fragment length(s) may be 3,178,201,223 bps INFO @ Sat, 08 Aug 2020 12:37:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.05_model.r INFO @ Sat, 08 Aug 2020 12:37:49: #3 Call peaks... INFO @ Sat, 08 Aug 2020 12:37:49: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Aug 2020 12:37:50: #3 Call peaks for each chromosome... INFO @ Sat, 08 Aug 2020 12:37:50: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.05_peaks.xls INFO @ Sat, 08 Aug 2020 12:37:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.05_peaks.narrowPeak INFO @ Sat, 08 Aug 2020 12:37:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.05_summits.bed INFO @ Sat, 08 Aug 2020 12:37:50: Done! pass1 - making usageList (1 chroms): 0 millis pass2 - checking and writing primary data (4 records, 4 fields): 2 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 12:38:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 12:38:10: #1 read tag files... INFO @ Sat, 08 Aug 2020 12:38:10: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 12:38:15: 1000000 INFO @ Sat, 08 Aug 2020 12:38:17: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 12:38:17: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 12:38:17: #1 total tags in treatment: 549245 INFO @ Sat, 08 Aug 2020 12:38:17: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 12:38:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 12:38:17: #1 tags after filtering in treatment: 469147 INFO @ Sat, 08 Aug 2020 12:38:17: #1 Redundant rate of treatment: 0.15 INFO @ Sat, 08 Aug 2020 12:38:17: #1 finished! INFO @ Sat, 08 Aug 2020 12:38:17: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 12:38:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 12:38:17: #2 number of paired peaks: 1762 INFO @ Sat, 08 Aug 2020 12:38:17: start model_add_line... INFO @ Sat, 08 Aug 2020 12:38:17: start X-correlation... INFO @ Sat, 08 Aug 2020 12:38:17: end of X-cor INFO @ Sat, 08 Aug 2020 12:38:17: #2 finished! INFO @ Sat, 08 Aug 2020 12:38:17: #2 predicted fragment length is 201 bps INFO @ Sat, 08 Aug 2020 12:38:17: #2 alternative fragment length(s) may be 3,178,201,223 bps INFO @ Sat, 08 Aug 2020 12:38:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.10_model.r INFO @ Sat, 08 Aug 2020 12:38:17: #3 Call peaks... INFO @ Sat, 08 Aug 2020 12:38:17: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Aug 2020 12:38:18: #3 Call peaks for each chromosome... INFO @ Sat, 08 Aug 2020 12:38:19: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.10_peaks.xls INFO @ Sat, 08 Aug 2020 12:38:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.10_peaks.narrowPeak INFO @ Sat, 08 Aug 2020 12:38:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.10_summits.bed INFO @ Sat, 08 Aug 2020 12:38:19: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 12:38:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 12:38:40: #1 read tag files... INFO @ Sat, 08 Aug 2020 12:38:40: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 08 Aug 2020 12:38:46: 1000000 BigWig に変換しました。 INFO @ Sat, 08 Aug 2020 12:38:47: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 12:38:47: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 12:38:47: #1 total tags in treatment: 549245 INFO @ Sat, 08 Aug 2020 12:38:47: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 12:38:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 12:38:47: #1 tags after filtering in treatment: 469147 INFO @ Sat, 08 Aug 2020 12:38:47: #1 Redundant rate of treatment: 0.15 INFO @ Sat, 08 Aug 2020 12:38:47: #1 finished! INFO @ Sat, 08 Aug 2020 12:38:47: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 12:38:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 12:38:47: #2 number of paired peaks: 1762 INFO @ Sat, 08 Aug 2020 12:38:47: start model_add_line... INFO @ Sat, 08 Aug 2020 12:38:47: start X-correlation... INFO @ Sat, 08 Aug 2020 12:38:47: end of X-cor INFO @ Sat, 08 Aug 2020 12:38:47: #2 finished! INFO @ Sat, 08 Aug 2020 12:38:47: #2 predicted fragment length is 201 bps INFO @ Sat, 08 Aug 2020 12:38:47: #2 alternative fragment length(s) may be 3,178,201,223 bps INFO @ Sat, 08 Aug 2020 12:38:47: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.20_model.r INFO @ Sat, 08 Aug 2020 12:38:47: #3 Call peaks... INFO @ Sat, 08 Aug 2020 12:38:47: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 08 Aug 2020 12:38:48: #3 Call peaks for each chromosome... INFO @ Sat, 08 Aug 2020 12:38:49: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.20_peaks.xls INFO @ Sat, 08 Aug 2020 12:38:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.20_peaks.narrowPeak INFO @ Sat, 08 Aug 2020 12:38:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7917372/SRX7917372.20_summits.bed INFO @ Sat, 08 Aug 2020 12:38:49: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling