Job ID = 8069507 SRX = SRX7917365 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-08-08T03:33:44 prefetch.2.10.7: 1) Downloading 'SRR11312912'... 2020-08-08T03:33:44 prefetch.2.10.7: Downloading via HTTPS... 2020-08-08T03:34:16 prefetch.2.10.7: HTTPS download succeed 2020-08-08T03:34:16 prefetch.2.10.7: 'SRR11312912' is valid 2020-08-08T03:34:16 prefetch.2.10.7: 1) 'SRR11312912' was downloaded successfully Read 2031585 spots for SRR11312912/SRR11312912.sra Written 2031585 spots for SRR11312912/SRR11312912.sra fastq に変換しました。 bowtie でマッピング中... Your job 8069827 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:58 2031585 reads; of these: 2031585 (100.00%) were paired; of these: 515529 (25.38%) aligned concordantly 0 times 1129495 (55.60%) aligned concordantly exactly 1 time 386561 (19.03%) aligned concordantly >1 times ---- 515529 pairs aligned concordantly 0 times; of these: 207515 (40.25%) aligned discordantly 1 time ---- 308014 pairs aligned 0 times concordantly or discordantly; of these: 616028 mates make up the pairs; of these: 313953 (50.96%) aligned 0 times 119983 (19.48%) aligned exactly 1 time 182092 (29.56%) aligned >1 times 92.27% overall alignment rate Time searching: 00:03:58 Overall time: 00:03:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 520017 / 1715306 = 0.3032 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 12:39:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 12:39:44: #1 read tag files... INFO @ Sat, 08 Aug 2020 12:39:44: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 12:39:49: 1000000 INFO @ Sat, 08 Aug 2020 12:39:53: 2000000 INFO @ Sat, 08 Aug 2020 12:39:56: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 12:39:56: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 12:39:56: #1 total tags in treatment: 1028827 INFO @ Sat, 08 Aug 2020 12:39:56: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 12:39:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 12:39:56: #1 tags after filtering in treatment: 876921 INFO @ Sat, 08 Aug 2020 12:39:56: #1 Redundant rate of treatment: 0.15 INFO @ Sat, 08 Aug 2020 12:39:56: #1 finished! INFO @ Sat, 08 Aug 2020 12:39:56: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 12:39:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 12:39:56: #2 number of paired peaks: 42 WARNING @ Sat, 08 Aug 2020 12:39:56: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Aug 2020 12:39:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 12:40:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 12:40:14: #1 read tag files... INFO @ Sat, 08 Aug 2020 12:40:14: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 12:40:19: 1000000 INFO @ Sat, 08 Aug 2020 12:40:23: 2000000 INFO @ Sat, 08 Aug 2020 12:40:27: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 12:40:27: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 12:40:27: #1 total tags in treatment: 1028827 INFO @ Sat, 08 Aug 2020 12:40:27: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 12:40:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 12:40:27: #1 tags after filtering in treatment: 876921 INFO @ Sat, 08 Aug 2020 12:40:27: #1 Redundant rate of treatment: 0.15 INFO @ Sat, 08 Aug 2020 12:40:27: #1 finished! INFO @ Sat, 08 Aug 2020 12:40:27: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 12:40:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 12:40:27: #2 number of paired peaks: 42 WARNING @ Sat, 08 Aug 2020 12:40:27: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Aug 2020 12:40:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 08 Aug 2020 12:40:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Aug 2020 12:40:44: #1 read tag files... INFO @ Sat, 08 Aug 2020 12:40:44: #1 read treatment tags... INFO @ Sat, 08 Aug 2020 12:40:49: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 08 Aug 2020 12:40:54: 2000000 INFO @ Sat, 08 Aug 2020 12:40:57: #1 tag size is determined as 62 bps INFO @ Sat, 08 Aug 2020 12:40:57: #1 tag size = 62 INFO @ Sat, 08 Aug 2020 12:40:57: #1 total tags in treatment: 1028827 INFO @ Sat, 08 Aug 2020 12:40:57: #1 user defined the maximum tags... INFO @ Sat, 08 Aug 2020 12:40:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Aug 2020 12:40:57: #1 tags after filtering in treatment: 876921 INFO @ Sat, 08 Aug 2020 12:40:57: #1 Redundant rate of treatment: 0.15 INFO @ Sat, 08 Aug 2020 12:40:57: #1 finished! INFO @ Sat, 08 Aug 2020 12:40:57: #2 Build Peak Model... INFO @ Sat, 08 Aug 2020 12:40:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Aug 2020 12:40:57: #2 number of paired peaks: 42 WARNING @ Sat, 08 Aug 2020 12:40:57: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Aug 2020 12:40:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7917365/SRX7917365.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。