Job ID = 14167255 SRX = SRX7848952 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 48813938 spots for SRR11237235/SRR11237235.sra Written 48813938 spots for SRR11237235/SRR11237235.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167906 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:15 48813938 reads; of these: 48813938 (100.00%) were unpaired; of these: 1598267 (3.27%) aligned 0 times 32237178 (66.04%) aligned exactly 1 time 14978493 (30.68%) aligned >1 times 96.73% overall alignment rate Time searching: 00:16:15 Overall time: 00:16:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 10585925 / 47215671 = 0.2242 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:46:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:46:52: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:46:52: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:46:57: 1000000 INFO @ Fri, 10 Dec 2021 11:47:02: 2000000 INFO @ Fri, 10 Dec 2021 11:47:08: 3000000 INFO @ Fri, 10 Dec 2021 11:47:13: 4000000 INFO @ Fri, 10 Dec 2021 11:47:18: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:47:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:47:21: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:47:21: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:47:24: 6000000 INFO @ Fri, 10 Dec 2021 11:47:28: 1000000 INFO @ Fri, 10 Dec 2021 11:47:30: 7000000 INFO @ Fri, 10 Dec 2021 11:47:34: 2000000 INFO @ Fri, 10 Dec 2021 11:47:36: 8000000 INFO @ Fri, 10 Dec 2021 11:47:40: 3000000 INFO @ Fri, 10 Dec 2021 11:47:41: 9000000 INFO @ Fri, 10 Dec 2021 11:47:46: 4000000 INFO @ Fri, 10 Dec 2021 11:47:47: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 11:47:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 11:47:52: #1 read tag files... INFO @ Fri, 10 Dec 2021 11:47:52: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 11:47:52: 5000000 INFO @ Fri, 10 Dec 2021 11:47:53: 11000000 INFO @ Fri, 10 Dec 2021 11:47:58: 6000000 INFO @ Fri, 10 Dec 2021 11:47:58: 1000000 INFO @ Fri, 10 Dec 2021 11:47:59: 12000000 INFO @ Fri, 10 Dec 2021 11:48:04: 7000000 INFO @ Fri, 10 Dec 2021 11:48:04: 2000000 INFO @ Fri, 10 Dec 2021 11:48:05: 13000000 INFO @ Fri, 10 Dec 2021 11:48:10: 8000000 INFO @ Fri, 10 Dec 2021 11:48:10: 3000000 INFO @ Fri, 10 Dec 2021 11:48:11: 14000000 INFO @ Fri, 10 Dec 2021 11:48:16: 9000000 INFO @ Fri, 10 Dec 2021 11:48:16: 4000000 INFO @ Fri, 10 Dec 2021 11:48:17: 15000000 INFO @ Fri, 10 Dec 2021 11:48:22: 10000000 INFO @ Fri, 10 Dec 2021 11:48:22: 5000000 INFO @ Fri, 10 Dec 2021 11:48:23: 16000000 INFO @ Fri, 10 Dec 2021 11:48:28: 11000000 INFO @ Fri, 10 Dec 2021 11:48:28: 6000000 INFO @ Fri, 10 Dec 2021 11:48:29: 17000000 INFO @ Fri, 10 Dec 2021 11:48:34: 12000000 INFO @ Fri, 10 Dec 2021 11:48:34: 7000000 INFO @ Fri, 10 Dec 2021 11:48:35: 18000000 INFO @ Fri, 10 Dec 2021 11:48:40: 13000000 INFO @ Fri, 10 Dec 2021 11:48:40: 8000000 INFO @ Fri, 10 Dec 2021 11:48:41: 19000000 INFO @ Fri, 10 Dec 2021 11:48:46: 14000000 INFO @ Fri, 10 Dec 2021 11:48:47: 9000000 INFO @ Fri, 10 Dec 2021 11:48:47: 20000000 INFO @ Fri, 10 Dec 2021 11:48:52: 15000000 INFO @ Fri, 10 Dec 2021 11:48:53: 10000000 INFO @ Fri, 10 Dec 2021 11:48:53: 21000000 INFO @ Fri, 10 Dec 2021 11:48:58: 16000000 INFO @ Fri, 10 Dec 2021 11:48:59: 11000000 INFO @ Fri, 10 Dec 2021 11:48:59: 22000000 INFO @ Fri, 10 Dec 2021 11:49:04: 17000000 INFO @ Fri, 10 Dec 2021 11:49:05: 12000000 INFO @ Fri, 10 Dec 2021 11:49:05: 23000000 INFO @ Fri, 10 Dec 2021 11:49:10: 18000000 INFO @ Fri, 10 Dec 2021 11:49:11: 13000000 INFO @ Fri, 10 Dec 2021 11:49:11: 24000000 INFO @ Fri, 10 Dec 2021 11:49:16: 19000000 INFO @ Fri, 10 Dec 2021 11:49:17: 14000000 INFO @ Fri, 10 Dec 2021 11:49:17: 25000000 INFO @ Fri, 10 Dec 2021 11:49:22: 20000000 INFO @ Fri, 10 Dec 2021 11:49:23: 26000000 INFO @ Fri, 10 Dec 2021 11:49:23: 15000000 INFO @ Fri, 10 Dec 2021 11:49:28: 21000000 INFO @ Fri, 10 Dec 2021 11:49:29: 27000000 INFO @ Fri, 10 Dec 2021 11:49:29: 16000000 INFO @ Fri, 10 Dec 2021 11:49:34: 22000000 INFO @ Fri, 10 Dec 2021 11:49:35: 28000000 INFO @ Fri, 10 Dec 2021 11:49:35: 17000000 INFO @ Fri, 10 Dec 2021 11:49:40: 23000000 INFO @ Fri, 10 Dec 2021 11:49:41: 29000000 INFO @ Fri, 10 Dec 2021 11:49:41: 18000000 INFO @ Fri, 10 Dec 2021 11:49:46: 24000000 INFO @ Fri, 10 Dec 2021 11:49:47: 30000000 INFO @ Fri, 10 Dec 2021 11:49:47: 19000000 INFO @ Fri, 10 Dec 2021 11:49:52: 25000000 INFO @ Fri, 10 Dec 2021 11:49:53: 31000000 INFO @ Fri, 10 Dec 2021 11:49:53: 20000000 INFO @ Fri, 10 Dec 2021 11:49:58: 26000000 INFO @ Fri, 10 Dec 2021 11:49:59: 32000000 INFO @ Fri, 10 Dec 2021 11:49:59: 21000000 INFO @ Fri, 10 Dec 2021 11:50:04: 27000000 INFO @ Fri, 10 Dec 2021 11:50:05: 33000000 INFO @ Fri, 10 Dec 2021 11:50:05: 22000000 INFO @ Fri, 10 Dec 2021 11:50:11: 28000000 INFO @ Fri, 10 Dec 2021 11:50:11: 34000000 INFO @ Fri, 10 Dec 2021 11:50:11: 23000000 INFO @ Fri, 10 Dec 2021 11:50:17: 29000000 INFO @ Fri, 10 Dec 2021 11:50:17: 35000000 INFO @ Fri, 10 Dec 2021 11:50:17: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 11:50:23: 30000000 INFO @ Fri, 10 Dec 2021 11:50:23: 36000000 INFO @ Fri, 10 Dec 2021 11:50:24: 25000000 INFO @ Fri, 10 Dec 2021 11:50:27: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 11:50:27: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 11:50:27: #1 total tags in treatment: 36629746 INFO @ Fri, 10 Dec 2021 11:50:27: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:50:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:50:28: #1 tags after filtering in treatment: 36629746 INFO @ Fri, 10 Dec 2021 11:50:28: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:50:28: #1 finished! INFO @ Fri, 10 Dec 2021 11:50:28: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:50:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:50:29: 31000000 INFO @ Fri, 10 Dec 2021 11:50:30: #2 number of paired peaks: 73 WARNING @ Fri, 10 Dec 2021 11:50:30: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:50:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.05_peaks.narrowPeak: No such file or directory INFO @ Fri, 10 Dec 2021 11:50:30: 26000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:50:35: 32000000 INFO @ Fri, 10 Dec 2021 11:50:36: 27000000 INFO @ Fri, 10 Dec 2021 11:50:42: 33000000 INFO @ Fri, 10 Dec 2021 11:50:42: 28000000 INFO @ Fri, 10 Dec 2021 11:50:48: 34000000 INFO @ Fri, 10 Dec 2021 11:50:49: 29000000 INFO @ Fri, 10 Dec 2021 11:50:54: 35000000 INFO @ Fri, 10 Dec 2021 11:50:55: 30000000 INFO @ Fri, 10 Dec 2021 11:51:00: 36000000 INFO @ Fri, 10 Dec 2021 11:51:01: 31000000 INFO @ Fri, 10 Dec 2021 11:51:04: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 11:51:04: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 11:51:04: #1 total tags in treatment: 36629746 INFO @ Fri, 10 Dec 2021 11:51:04: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:51:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:51:04: #1 tags after filtering in treatment: 36629746 INFO @ Fri, 10 Dec 2021 11:51:04: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:51:04: #1 finished! INFO @ Fri, 10 Dec 2021 11:51:04: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:51:04: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 11:51:06: #2 number of paired peaks: 73 WARNING @ Fri, 10 Dec 2021 11:51:06: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:51:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 11:51:07: 32000000 INFO @ Fri, 10 Dec 2021 11:51:12: 33000000 INFO @ Fri, 10 Dec 2021 11:51:17: 34000000 INFO @ Fri, 10 Dec 2021 11:51:23: 35000000 INFO @ Fri, 10 Dec 2021 11:51:28: 36000000 INFO @ Fri, 10 Dec 2021 11:51:31: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 11:51:31: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 11:51:31: #1 total tags in treatment: 36629746 INFO @ Fri, 10 Dec 2021 11:51:31: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 11:51:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 11:51:32: #1 tags after filtering in treatment: 36629746 INFO @ Fri, 10 Dec 2021 11:51:32: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 11:51:32: #1 finished! INFO @ Fri, 10 Dec 2021 11:51:32: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 11:51:32: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 11:51:34: #2 number of paired peaks: 73 WARNING @ Fri, 10 Dec 2021 11:51:34: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 11:51:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7848952/SRX7848952.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling