Job ID = 6626972 SRX = SRX7780067 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T00:06:54 prefetch.2.10.7: 1) Downloading 'SRR11143822'... 2020-07-14T00:06:54 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T00:10:15 prefetch.2.10.7: HTTPS download succeed 2020-07-14T00:10:15 prefetch.2.10.7: 1) 'SRR11143822' was downloaded successfully Read 21963915 spots for SRR11143822/SRR11143822.sra Written 21963915 spots for SRR11143822/SRR11143822.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627130 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:03 21963915 reads; of these: 21963915 (100.00%) were unpaired; of these: 1891213 (8.61%) aligned 0 times 13711498 (62.43%) aligned exactly 1 time 6361204 (28.96%) aligned >1 times 91.39% overall alignment rate Time searching: 00:07:03 Overall time: 00:07:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3955801 / 20072702 = 0.1971 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:22:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:22:45: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:22:45: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:22:50: 1000000 INFO @ Tue, 14 Jul 2020 09:22:55: 2000000 INFO @ Tue, 14 Jul 2020 09:22:59: 3000000 INFO @ Tue, 14 Jul 2020 09:23:04: 4000000 INFO @ Tue, 14 Jul 2020 09:23:09: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:23:13: 6000000 INFO @ Tue, 14 Jul 2020 09:23:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:23:15: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:23:15: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:23:18: 7000000 INFO @ Tue, 14 Jul 2020 09:23:23: 1000000 INFO @ Tue, 14 Jul 2020 09:23:23: 8000000 INFO @ Tue, 14 Jul 2020 09:23:28: 9000000 INFO @ Tue, 14 Jul 2020 09:23:28: 2000000 INFO @ Tue, 14 Jul 2020 09:23:33: 10000000 INFO @ Tue, 14 Jul 2020 09:23:34: 3000000 INFO @ Tue, 14 Jul 2020 09:23:38: 11000000 INFO @ Tue, 14 Jul 2020 09:23:40: 4000000 INFO @ Tue, 14 Jul 2020 09:23:42: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:23:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:23:45: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:23:45: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:23:45: 5000000 INFO @ Tue, 14 Jul 2020 09:23:47: 13000000 INFO @ Tue, 14 Jul 2020 09:23:50: 1000000 INFO @ Tue, 14 Jul 2020 09:23:51: 6000000 INFO @ Tue, 14 Jul 2020 09:23:52: 14000000 INFO @ Tue, 14 Jul 2020 09:23:55: 2000000 INFO @ Tue, 14 Jul 2020 09:23:56: 7000000 INFO @ Tue, 14 Jul 2020 09:23:57: 15000000 INFO @ Tue, 14 Jul 2020 09:24:00: 3000000 INFO @ Tue, 14 Jul 2020 09:24:02: 16000000 INFO @ Tue, 14 Jul 2020 09:24:02: 8000000 INFO @ Tue, 14 Jul 2020 09:24:02: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:24:02: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:24:02: #1 total tags in treatment: 16116901 INFO @ Tue, 14 Jul 2020 09:24:02: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:24:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:24:03: #1 tags after filtering in treatment: 16116901 INFO @ Tue, 14 Jul 2020 09:24:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:24:03: #1 finished! INFO @ Tue, 14 Jul 2020 09:24:03: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:24:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:24:04: #2 number of paired peaks: 19 WARNING @ Tue, 14 Jul 2020 09:24:04: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:24:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:24:05: 4000000 INFO @ Tue, 14 Jul 2020 09:24:08: 9000000 INFO @ Tue, 14 Jul 2020 09:24:10: 5000000 INFO @ Tue, 14 Jul 2020 09:24:13: 10000000 INFO @ Tue, 14 Jul 2020 09:24:14: 6000000 INFO @ Tue, 14 Jul 2020 09:24:19: 11000000 INFO @ Tue, 14 Jul 2020 09:24:19: 7000000 INFO @ Tue, 14 Jul 2020 09:24:24: 12000000 INFO @ Tue, 14 Jul 2020 09:24:24: 8000000 INFO @ Tue, 14 Jul 2020 09:24:29: 9000000 INFO @ Tue, 14 Jul 2020 09:24:30: 13000000 INFO @ Tue, 14 Jul 2020 09:24:34: 10000000 INFO @ Tue, 14 Jul 2020 09:24:35: 14000000 INFO @ Tue, 14 Jul 2020 09:24:39: 11000000 INFO @ Tue, 14 Jul 2020 09:24:41: 15000000 INFO @ Tue, 14 Jul 2020 09:24:44: 12000000 INFO @ Tue, 14 Jul 2020 09:24:46: 16000000 INFO @ Tue, 14 Jul 2020 09:24:47: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:24:47: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:24:47: #1 total tags in treatment: 16116901 INFO @ Tue, 14 Jul 2020 09:24:47: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:24:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:24:47: #1 tags after filtering in treatment: 16116901 INFO @ Tue, 14 Jul 2020 09:24:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:24:47: #1 finished! INFO @ Tue, 14 Jul 2020 09:24:47: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:24:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:24:48: #2 number of paired peaks: 19 WARNING @ Tue, 14 Jul 2020 09:24:48: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:24:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:24:49: 13000000 INFO @ Tue, 14 Jul 2020 09:24:53: 14000000 INFO @ Tue, 14 Jul 2020 09:24:58: 15000000 INFO @ Tue, 14 Jul 2020 09:25:03: 16000000 INFO @ Tue, 14 Jul 2020 09:25:04: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:25:04: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:25:04: #1 total tags in treatment: 16116901 INFO @ Tue, 14 Jul 2020 09:25:04: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:25:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:25:04: #1 tags after filtering in treatment: 16116901 INFO @ Tue, 14 Jul 2020 09:25:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:25:04: #1 finished! INFO @ Tue, 14 Jul 2020 09:25:04: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:25:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:25:05: #2 number of paired peaks: 19 WARNING @ Tue, 14 Jul 2020 09:25:05: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:25:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780067/SRX7780067.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。