Job ID = 6626968 SRX = SRX7780064 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T00:04:06 prefetch.2.10.7: 1) Downloading 'SRR11143819'... 2020-07-14T00:04:06 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T00:07:35 prefetch.2.10.7: HTTPS download succeed 2020-07-14T00:07:35 prefetch.2.10.7: 1) 'SRR11143819' was downloaded successfully Read 24973530 spots for SRR11143819/SRR11143819.sra Written 24973530 spots for SRR11143819/SRR11143819.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627124 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:07 24973530 reads; of these: 24973530 (100.00%) were unpaired; of these: 2727806 (10.92%) aligned 0 times 16330167 (65.39%) aligned exactly 1 time 5915557 (23.69%) aligned >1 times 89.08% overall alignment rate Time searching: 00:07:07 Overall time: 00:07:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4456389 / 22245724 = 0.2003 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:20:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:20:37: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:20:37: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:20:42: 1000000 INFO @ Tue, 14 Jul 2020 09:20:48: 2000000 INFO @ Tue, 14 Jul 2020 09:20:53: 3000000 INFO @ Tue, 14 Jul 2020 09:20:59: 4000000 INFO @ Tue, 14 Jul 2020 09:21:04: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:21:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:21:07: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:21:07: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:21:10: 6000000 INFO @ Tue, 14 Jul 2020 09:21:13: 1000000 INFO @ Tue, 14 Jul 2020 09:21:16: 7000000 INFO @ Tue, 14 Jul 2020 09:21:19: 2000000 INFO @ Tue, 14 Jul 2020 09:21:22: 8000000 INFO @ Tue, 14 Jul 2020 09:21:25: 3000000 INFO @ Tue, 14 Jul 2020 09:21:28: 9000000 INFO @ Tue, 14 Jul 2020 09:21:31: 4000000 INFO @ Tue, 14 Jul 2020 09:21:34: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:21:37: 5000000 INFO @ Tue, 14 Jul 2020 09:21:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:21:37: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:21:37: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:21:40: 11000000 INFO @ Tue, 14 Jul 2020 09:21:43: 6000000 INFO @ Tue, 14 Jul 2020 09:21:44: 1000000 INFO @ Tue, 14 Jul 2020 09:21:47: 12000000 INFO @ Tue, 14 Jul 2020 09:21:50: 7000000 INFO @ Tue, 14 Jul 2020 09:21:51: 2000000 INFO @ Tue, 14 Jul 2020 09:21:53: 13000000 INFO @ Tue, 14 Jul 2020 09:21:56: 8000000 INFO @ Tue, 14 Jul 2020 09:21:58: 3000000 INFO @ Tue, 14 Jul 2020 09:22:00: 14000000 INFO @ Tue, 14 Jul 2020 09:22:03: 9000000 INFO @ Tue, 14 Jul 2020 09:22:05: 4000000 INFO @ Tue, 14 Jul 2020 09:22:06: 15000000 INFO @ Tue, 14 Jul 2020 09:22:09: 10000000 INFO @ Tue, 14 Jul 2020 09:22:12: 5000000 INFO @ Tue, 14 Jul 2020 09:22:13: 16000000 INFO @ Tue, 14 Jul 2020 09:22:16: 11000000 INFO @ Tue, 14 Jul 2020 09:22:19: 6000000 INFO @ Tue, 14 Jul 2020 09:22:19: 17000000 INFO @ Tue, 14 Jul 2020 09:22:22: 12000000 INFO @ Tue, 14 Jul 2020 09:22:25: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:22:25: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:22:25: #1 total tags in treatment: 17789335 INFO @ Tue, 14 Jul 2020 09:22:25: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:22:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:22:25: #1 tags after filtering in treatment: 17789335 INFO @ Tue, 14 Jul 2020 09:22:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:22:25: #1 finished! INFO @ Tue, 14 Jul 2020 09:22:25: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:22:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:22:26: 7000000 INFO @ Tue, 14 Jul 2020 09:22:26: #2 number of paired peaks: 11 WARNING @ Tue, 14 Jul 2020 09:22:26: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:22:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:22:28: 13000000 INFO @ Tue, 14 Jul 2020 09:22:32: 8000000 INFO @ Tue, 14 Jul 2020 09:22:35: 14000000 INFO @ Tue, 14 Jul 2020 09:22:39: 9000000 INFO @ Tue, 14 Jul 2020 09:22:41: 15000000 INFO @ Tue, 14 Jul 2020 09:22:46: 10000000 INFO @ Tue, 14 Jul 2020 09:22:47: 16000000 INFO @ Tue, 14 Jul 2020 09:22:53: 11000000 INFO @ Tue, 14 Jul 2020 09:22:53: 17000000 INFO @ Tue, 14 Jul 2020 09:22:58: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:22:58: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:22:58: #1 total tags in treatment: 17789335 INFO @ Tue, 14 Jul 2020 09:22:58: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:22:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:22:59: #1 tags after filtering in treatment: 17789335 INFO @ Tue, 14 Jul 2020 09:22:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:22:59: #1 finished! INFO @ Tue, 14 Jul 2020 09:22:59: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:22:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:23:00: 12000000 INFO @ Tue, 14 Jul 2020 09:23:00: #2 number of paired peaks: 11 WARNING @ Tue, 14 Jul 2020 09:23:00: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:23:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:23:06: 13000000 INFO @ Tue, 14 Jul 2020 09:23:12: 14000000 INFO @ Tue, 14 Jul 2020 09:23:19: 15000000 INFO @ Tue, 14 Jul 2020 09:23:25: 16000000 INFO @ Tue, 14 Jul 2020 09:23:31: 17000000 INFO @ Tue, 14 Jul 2020 09:23:36: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:23:36: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:23:36: #1 total tags in treatment: 17789335 INFO @ Tue, 14 Jul 2020 09:23:36: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:23:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:23:37: #1 tags after filtering in treatment: 17789335 INFO @ Tue, 14 Jul 2020 09:23:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:23:37: #1 finished! INFO @ Tue, 14 Jul 2020 09:23:37: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:23:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:23:38: #2 number of paired peaks: 11 WARNING @ Tue, 14 Jul 2020 09:23:38: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:23:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780064/SRX7780064.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。