Job ID = 6626966 SRX = SRX7780062 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 25736001 spots for SRR11143817/SRR11143817.sra Written 25736001 spots for SRR11143817/SRR11143817.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627108 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:29 25736001 reads; of these: 25736001 (100.00%) were unpaired; of these: 2987854 (11.61%) aligned 0 times 16052415 (62.37%) aligned exactly 1 time 6695732 (26.02%) aligned >1 times 88.39% overall alignment rate Time searching: 00:07:29 Overall time: 00:07:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4955946 / 22748147 = 0.2179 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:16:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:16:21: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:16:21: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:16:27: 1000000 INFO @ Tue, 14 Jul 2020 09:16:32: 2000000 INFO @ Tue, 14 Jul 2020 09:16:38: 3000000 INFO @ Tue, 14 Jul 2020 09:16:43: 4000000 INFO @ Tue, 14 Jul 2020 09:16:48: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:16:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:16:51: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:16:51: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:16:54: 6000000 INFO @ Tue, 14 Jul 2020 09:16:59: 1000000 INFO @ Tue, 14 Jul 2020 09:17:01: 7000000 INFO @ Tue, 14 Jul 2020 09:17:06: 2000000 INFO @ Tue, 14 Jul 2020 09:17:07: 8000000 INFO @ Tue, 14 Jul 2020 09:17:13: 3000000 INFO @ Tue, 14 Jul 2020 09:17:14: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:17:20: 10000000 INFO @ Tue, 14 Jul 2020 09:17:20: 4000000 INFO @ Tue, 14 Jul 2020 09:17:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:17:21: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:17:21: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:17:27: 11000000 INFO @ Tue, 14 Jul 2020 09:17:28: 5000000 INFO @ Tue, 14 Jul 2020 09:17:28: 1000000 INFO @ Tue, 14 Jul 2020 09:17:34: 12000000 INFO @ Tue, 14 Jul 2020 09:17:35: 6000000 INFO @ Tue, 14 Jul 2020 09:17:35: 2000000 INFO @ Tue, 14 Jul 2020 09:17:41: 13000000 INFO @ Tue, 14 Jul 2020 09:17:42: 3000000 INFO @ Tue, 14 Jul 2020 09:17:42: 7000000 INFO @ Tue, 14 Jul 2020 09:17:47: 14000000 INFO @ Tue, 14 Jul 2020 09:17:49: 4000000 INFO @ Tue, 14 Jul 2020 09:17:49: 8000000 INFO @ Tue, 14 Jul 2020 09:17:54: 15000000 INFO @ Tue, 14 Jul 2020 09:17:55: 5000000 INFO @ Tue, 14 Jul 2020 09:17:56: 9000000 INFO @ Tue, 14 Jul 2020 09:18:01: 16000000 INFO @ Tue, 14 Jul 2020 09:18:02: 6000000 INFO @ Tue, 14 Jul 2020 09:18:04: 10000000 INFO @ Tue, 14 Jul 2020 09:18:08: 17000000 INFO @ Tue, 14 Jul 2020 09:18:09: 7000000 INFO @ Tue, 14 Jul 2020 09:18:11: 11000000 INFO @ Tue, 14 Jul 2020 09:18:13: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:18:13: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:18:13: #1 total tags in treatment: 17792201 INFO @ Tue, 14 Jul 2020 09:18:13: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:18:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:18:14: #1 tags after filtering in treatment: 17792201 INFO @ Tue, 14 Jul 2020 09:18:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:18:14: #1 finished! INFO @ Tue, 14 Jul 2020 09:18:14: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:18:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:18:15: #2 number of paired peaks: 30 WARNING @ Tue, 14 Jul 2020 09:18:15: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:18:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:18:16: 8000000 INFO @ Tue, 14 Jul 2020 09:18:18: 12000000 INFO @ Tue, 14 Jul 2020 09:18:22: 9000000 INFO @ Tue, 14 Jul 2020 09:18:25: 13000000 INFO @ Tue, 14 Jul 2020 09:18:29: 10000000 INFO @ Tue, 14 Jul 2020 09:18:32: 14000000 INFO @ Tue, 14 Jul 2020 09:18:35: 11000000 INFO @ Tue, 14 Jul 2020 09:18:40: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:18:42: 12000000 INFO @ Tue, 14 Jul 2020 09:18:47: 16000000 INFO @ Tue, 14 Jul 2020 09:18:48: 13000000 INFO @ Tue, 14 Jul 2020 09:18:54: 17000000 INFO @ Tue, 14 Jul 2020 09:18:55: 14000000 INFO @ Tue, 14 Jul 2020 09:19:00: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:19:00: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:19:00: #1 total tags in treatment: 17792201 INFO @ Tue, 14 Jul 2020 09:19:00: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:19:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:19:00: #1 tags after filtering in treatment: 17792201 INFO @ Tue, 14 Jul 2020 09:19:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:19:00: #1 finished! INFO @ Tue, 14 Jul 2020 09:19:00: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:19:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:19:01: #2 number of paired peaks: 30 WARNING @ Tue, 14 Jul 2020 09:19:01: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:19:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:19:01: 15000000 INFO @ Tue, 14 Jul 2020 09:19:07: 16000000 INFO @ Tue, 14 Jul 2020 09:19:14: 17000000 INFO @ Tue, 14 Jul 2020 09:19:19: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:19:19: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:19:19: #1 total tags in treatment: 17792201 INFO @ Tue, 14 Jul 2020 09:19:19: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:19:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:19:19: #1 tags after filtering in treatment: 17792201 INFO @ Tue, 14 Jul 2020 09:19:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:19:19: #1 finished! INFO @ Tue, 14 Jul 2020 09:19:19: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:19:19: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 09:19:20: #2 number of paired peaks: 30 WARNING @ Tue, 14 Jul 2020 09:19:20: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:19:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780062/SRX7780062.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling