Job ID = 6626965 SRX = SRX7780061 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T00:02:57 prefetch.2.10.7: 1) Downloading 'SRR11143816'... 2020-07-14T00:02:57 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T00:11:01 prefetch.2.10.7: HTTPS download failed 2020-07-14T00:11:01 prefetch.2.10.7: 1) failed to download SRR11143816 2020-07-14T00:11:11 prefetch.2.10.7: 1) Downloading 'SRR11143816'... 2020-07-14T00:11:11 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T00:11:11 prefetch.2.10.7: Continue download of 'SRR11143816' from 1211721681 2020-07-14T00:11:15 prefetch.2.10.7: HTTPS download succeed 2020-07-14T00:11:15 prefetch.2.10.7: 1) 'SRR11143816' was downloaded successfully Read 24110038 spots for SRR11143816/SRR11143816.sra Written 24110038 spots for SRR11143816/SRR11143816.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627134 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:38 24110038 reads; of these: 24110038 (100.00%) were unpaired; of these: 1928980 (8.00%) aligned 0 times 16604189 (68.87%) aligned exactly 1 time 5576869 (23.13%) aligned >1 times 92.00% overall alignment rate Time searching: 00:06:38 Overall time: 00:06:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4517522 / 22181058 = 0.2037 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:23:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:23:44: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:23:44: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:23:50: 1000000 INFO @ Tue, 14 Jul 2020 09:23:57: 2000000 INFO @ Tue, 14 Jul 2020 09:24:03: 3000000 INFO @ Tue, 14 Jul 2020 09:24:09: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:24:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:24:14: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:24:14: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:24:16: 5000000 INFO @ Tue, 14 Jul 2020 09:24:20: 1000000 INFO @ Tue, 14 Jul 2020 09:24:23: 6000000 INFO @ Tue, 14 Jul 2020 09:24:26: 2000000 INFO @ Tue, 14 Jul 2020 09:24:30: 7000000 INFO @ Tue, 14 Jul 2020 09:24:32: 3000000 INFO @ Tue, 14 Jul 2020 09:24:36: 8000000 INFO @ Tue, 14 Jul 2020 09:24:38: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:24:43: 9000000 INFO @ Tue, 14 Jul 2020 09:24:44: 5000000 INFO @ Tue, 14 Jul 2020 09:24:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:24:44: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:24:44: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:24:50: 10000000 INFO @ Tue, 14 Jul 2020 09:24:50: 6000000 INFO @ Tue, 14 Jul 2020 09:24:50: 1000000 INFO @ Tue, 14 Jul 2020 09:24:56: 7000000 INFO @ Tue, 14 Jul 2020 09:24:56: 2000000 INFO @ Tue, 14 Jul 2020 09:24:57: 11000000 INFO @ Tue, 14 Jul 2020 09:25:02: 8000000 INFO @ Tue, 14 Jul 2020 09:25:02: 3000000 INFO @ Tue, 14 Jul 2020 09:25:04: 12000000 INFO @ Tue, 14 Jul 2020 09:25:08: 9000000 INFO @ Tue, 14 Jul 2020 09:25:08: 4000000 INFO @ Tue, 14 Jul 2020 09:25:10: 13000000 INFO @ Tue, 14 Jul 2020 09:25:15: 5000000 INFO @ Tue, 14 Jul 2020 09:25:15: 10000000 INFO @ Tue, 14 Jul 2020 09:25:17: 14000000 INFO @ Tue, 14 Jul 2020 09:25:21: 11000000 INFO @ Tue, 14 Jul 2020 09:25:21: 6000000 INFO @ Tue, 14 Jul 2020 09:25:24: 15000000 INFO @ Tue, 14 Jul 2020 09:25:27: 7000000 INFO @ Tue, 14 Jul 2020 09:25:27: 12000000 INFO @ Tue, 14 Jul 2020 09:25:31: 16000000 INFO @ Tue, 14 Jul 2020 09:25:33: 8000000 INFO @ Tue, 14 Jul 2020 09:25:33: 13000000 INFO @ Tue, 14 Jul 2020 09:25:38: 17000000 INFO @ Tue, 14 Jul 2020 09:25:39: 9000000 INFO @ Tue, 14 Jul 2020 09:25:39: 14000000 INFO @ Tue, 14 Jul 2020 09:25:43: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:25:43: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:25:43: #1 total tags in treatment: 17663536 INFO @ Tue, 14 Jul 2020 09:25:43: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:25:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:25:43: #1 tags after filtering in treatment: 17663536 INFO @ Tue, 14 Jul 2020 09:25:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:25:43: #1 finished! INFO @ Tue, 14 Jul 2020 09:25:43: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:25:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:25:44: #2 number of paired peaks: 7 WARNING @ Tue, 14 Jul 2020 09:25:44: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:25:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:25:45: 10000000 INFO @ Tue, 14 Jul 2020 09:25:46: 15000000 INFO @ Tue, 14 Jul 2020 09:25:51: 11000000 INFO @ Tue, 14 Jul 2020 09:25:52: 16000000 INFO @ Tue, 14 Jul 2020 09:25:57: 12000000 INFO @ Tue, 14 Jul 2020 09:25:57: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:26:01: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:26:01: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:26:01: #1 total tags in treatment: 17663536 INFO @ Tue, 14 Jul 2020 09:26:01: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:26:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:26:02: #1 tags after filtering in treatment: 17663536 INFO @ Tue, 14 Jul 2020 09:26:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:26:02: #1 finished! INFO @ Tue, 14 Jul 2020 09:26:02: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:26:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:26:03: #2 number of paired peaks: 7 WARNING @ Tue, 14 Jul 2020 09:26:03: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:26:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:26:03: 13000000 INFO @ Tue, 14 Jul 2020 09:26:09: 14000000 INFO @ Tue, 14 Jul 2020 09:26:14: 15000000 INFO @ Tue, 14 Jul 2020 09:26:20: 16000000 INFO @ Tue, 14 Jul 2020 09:26:26: 17000000 INFO @ Tue, 14 Jul 2020 09:26:29: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:26:29: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:26:29: #1 total tags in treatment: 17663536 INFO @ Tue, 14 Jul 2020 09:26:29: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:26:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:26:30: #1 tags after filtering in treatment: 17663536 INFO @ Tue, 14 Jul 2020 09:26:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:26:30: #1 finished! INFO @ Tue, 14 Jul 2020 09:26:30: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:26:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:26:31: #2 number of paired peaks: 7 WARNING @ Tue, 14 Jul 2020 09:26:31: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:26:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780061/SRX7780061.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。