Job ID = 6626960 SRX = SRX7780059 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-14T00:02:51 prefetch.2.10.7: 1) Downloading 'SRR11143814'... 2020-07-14T00:02:51 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T00:06:34 prefetch.2.10.7: HTTPS download succeed 2020-07-14T00:06:34 prefetch.2.10.7: 1) 'SRR11143814' was downloaded successfully Read 42379724 spots for SRR11143814/SRR11143814.sra Written 42379724 spots for SRR11143814/SRR11143814.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627140 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:01 42379724 reads; of these: 42379724 (100.00%) were unpaired; of these: 3731226 (8.80%) aligned 0 times 28953824 (68.32%) aligned exactly 1 time 9694674 (22.88%) aligned >1 times 91.20% overall alignment rate Time searching: 00:13:01 Overall time: 00:13:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 11672013 / 38648498 = 0.3020 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:30:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:30:07: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:30:07: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:30:13: 1000000 INFO @ Tue, 14 Jul 2020 09:30:19: 2000000 INFO @ Tue, 14 Jul 2020 09:30:25: 3000000 INFO @ Tue, 14 Jul 2020 09:30:31: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:30:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:30:37: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:30:37: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:30:37: 5000000 INFO @ Tue, 14 Jul 2020 09:30:44: 1000000 INFO @ Tue, 14 Jul 2020 09:30:44: 6000000 INFO @ Tue, 14 Jul 2020 09:30:50: 2000000 INFO @ Tue, 14 Jul 2020 09:30:51: 7000000 INFO @ Tue, 14 Jul 2020 09:30:57: 3000000 INFO @ Tue, 14 Jul 2020 09:30:57: 8000000 INFO @ Tue, 14 Jul 2020 09:31:03: 4000000 INFO @ Tue, 14 Jul 2020 09:31:04: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:31:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:31:07: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:31:07: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:31:10: 5000000 INFO @ Tue, 14 Jul 2020 09:31:10: 10000000 INFO @ Tue, 14 Jul 2020 09:31:14: 1000000 INFO @ Tue, 14 Jul 2020 09:31:17: 6000000 INFO @ Tue, 14 Jul 2020 09:31:17: 11000000 INFO @ Tue, 14 Jul 2020 09:31:21: 2000000 INFO @ Tue, 14 Jul 2020 09:31:23: 7000000 INFO @ Tue, 14 Jul 2020 09:31:24: 12000000 INFO @ Tue, 14 Jul 2020 09:31:28: 3000000 INFO @ Tue, 14 Jul 2020 09:31:30: 8000000 INFO @ Tue, 14 Jul 2020 09:31:30: 13000000 INFO @ Tue, 14 Jul 2020 09:31:35: 4000000 INFO @ Tue, 14 Jul 2020 09:31:37: 9000000 INFO @ Tue, 14 Jul 2020 09:31:37: 14000000 INFO @ Tue, 14 Jul 2020 09:31:42: 5000000 INFO @ Tue, 14 Jul 2020 09:31:43: 10000000 INFO @ Tue, 14 Jul 2020 09:31:44: 15000000 INFO @ Tue, 14 Jul 2020 09:31:48: 6000000 INFO @ Tue, 14 Jul 2020 09:31:50: 11000000 INFO @ Tue, 14 Jul 2020 09:31:51: 16000000 INFO @ Tue, 14 Jul 2020 09:31:55: 7000000 INFO @ Tue, 14 Jul 2020 09:31:56: 12000000 INFO @ Tue, 14 Jul 2020 09:31:57: 17000000 INFO @ Tue, 14 Jul 2020 09:32:02: 8000000 INFO @ Tue, 14 Jul 2020 09:32:03: 13000000 INFO @ Tue, 14 Jul 2020 09:32:04: 18000000 INFO @ Tue, 14 Jul 2020 09:32:09: 9000000 INFO @ Tue, 14 Jul 2020 09:32:09: 14000000 INFO @ Tue, 14 Jul 2020 09:32:11: 19000000 INFO @ Tue, 14 Jul 2020 09:32:16: 10000000 INFO @ Tue, 14 Jul 2020 09:32:16: 15000000 INFO @ Tue, 14 Jul 2020 09:32:17: 20000000 INFO @ Tue, 14 Jul 2020 09:32:22: 11000000 INFO @ Tue, 14 Jul 2020 09:32:23: 16000000 INFO @ Tue, 14 Jul 2020 09:32:24: 21000000 INFO @ Tue, 14 Jul 2020 09:32:29: 12000000 INFO @ Tue, 14 Jul 2020 09:32:29: 17000000 INFO @ Tue, 14 Jul 2020 09:32:31: 22000000 INFO @ Tue, 14 Jul 2020 09:32:36: 18000000 INFO @ Tue, 14 Jul 2020 09:32:36: 13000000 INFO @ Tue, 14 Jul 2020 09:32:38: 23000000 INFO @ Tue, 14 Jul 2020 09:32:42: 19000000 INFO @ Tue, 14 Jul 2020 09:32:42: 14000000 INFO @ Tue, 14 Jul 2020 09:32:45: 24000000 INFO @ Tue, 14 Jul 2020 09:32:49: 20000000 INFO @ Tue, 14 Jul 2020 09:32:49: 15000000 INFO @ Tue, 14 Jul 2020 09:32:51: 25000000 INFO @ Tue, 14 Jul 2020 09:32:56: 21000000 INFO @ Tue, 14 Jul 2020 09:32:56: 16000000 INFO @ Tue, 14 Jul 2020 09:32:58: 26000000 INFO @ Tue, 14 Jul 2020 09:33:02: 22000000 INFO @ Tue, 14 Jul 2020 09:33:03: 17000000 INFO @ Tue, 14 Jul 2020 09:33:05: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:33:05: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:33:05: #1 total tags in treatment: 26976485 INFO @ Tue, 14 Jul 2020 09:33:05: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:33:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:33:06: #1 tags after filtering in treatment: 26976485 INFO @ Tue, 14 Jul 2020 09:33:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:33:06: #1 finished! INFO @ Tue, 14 Jul 2020 09:33:06: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:33:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:33:08: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 09:33:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:33:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:33:09: 23000000 INFO @ Tue, 14 Jul 2020 09:33:09: 18000000 INFO @ Tue, 14 Jul 2020 09:33:16: 24000000 INFO @ Tue, 14 Jul 2020 09:33:16: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:33:22: 25000000 INFO @ Tue, 14 Jul 2020 09:33:23: 20000000 INFO @ Tue, 14 Jul 2020 09:33:29: 26000000 INFO @ Tue, 14 Jul 2020 09:33:29: 21000000 INFO @ Tue, 14 Jul 2020 09:33:35: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:33:35: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:33:35: #1 total tags in treatment: 26976485 INFO @ Tue, 14 Jul 2020 09:33:35: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:33:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:33:36: #1 tags after filtering in treatment: 26976485 INFO @ Tue, 14 Jul 2020 09:33:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:33:36: #1 finished! INFO @ Tue, 14 Jul 2020 09:33:36: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:33:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:33:36: 22000000 INFO @ Tue, 14 Jul 2020 09:33:38: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 09:33:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:33:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:33:43: 23000000 INFO @ Tue, 14 Jul 2020 09:33:49: 24000000 INFO @ Tue, 14 Jul 2020 09:33:55: 25000000 INFO @ Tue, 14 Jul 2020 09:34:02: 26000000 INFO @ Tue, 14 Jul 2020 09:34:08: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:34:08: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:34:08: #1 total tags in treatment: 26976485 INFO @ Tue, 14 Jul 2020 09:34:08: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:34:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:34:09: #1 tags after filtering in treatment: 26976485 INFO @ Tue, 14 Jul 2020 09:34:09: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:34:09: #1 finished! INFO @ Tue, 14 Jul 2020 09:34:09: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:34:09: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:34:11: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 09:34:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:34:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780059/SRX7780059.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。