Job ID = 6626952 SRX = SRX7780057 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-13T23:59:17 prefetch.2.10.7: 1) Downloading 'SRR11143812'... 2020-07-13T23:59:17 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T00:00:39 prefetch.2.10.7: HTTPS download succeed 2020-07-14T00:00:39 prefetch.2.10.7: 1) 'SRR11143812' was downloaded successfully Read 28771525 spots for SRR11143812/SRR11143812.sra Written 28771525 spots for SRR11143812/SRR11143812.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627106 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:22 28771525 reads; of these: 28771525 (100.00%) were unpaired; of these: 1889947 (6.57%) aligned 0 times 19707120 (68.50%) aligned exactly 1 time 7174458 (24.94%) aligned >1 times 93.43% overall alignment rate Time searching: 00:08:22 Overall time: 00:08:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6129270 / 26881578 = 0.2280 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:15:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:15:42: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:15:42: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:15:48: 1000000 INFO @ Tue, 14 Jul 2020 09:15:54: 2000000 INFO @ Tue, 14 Jul 2020 09:16:00: 3000000 INFO @ Tue, 14 Jul 2020 09:16:06: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:16:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:16:12: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:16:12: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:16:12: 5000000 INFO @ Tue, 14 Jul 2020 09:16:18: 1000000 INFO @ Tue, 14 Jul 2020 09:16:18: 6000000 INFO @ Tue, 14 Jul 2020 09:16:23: 2000000 INFO @ Tue, 14 Jul 2020 09:16:25: 7000000 INFO @ Tue, 14 Jul 2020 09:16:29: 3000000 INFO @ Tue, 14 Jul 2020 09:16:31: 8000000 INFO @ Tue, 14 Jul 2020 09:16:36: 4000000 INFO @ Tue, 14 Jul 2020 09:16:38: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:16:41: 5000000 INFO @ Tue, 14 Jul 2020 09:16:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:16:42: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:16:42: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:16:44: 10000000 INFO @ Tue, 14 Jul 2020 09:16:47: 6000000 INFO @ Tue, 14 Jul 2020 09:16:48: 1000000 INFO @ Tue, 14 Jul 2020 09:16:51: 11000000 INFO @ Tue, 14 Jul 2020 09:16:53: 7000000 INFO @ Tue, 14 Jul 2020 09:16:55: 2000000 INFO @ Tue, 14 Jul 2020 09:16:57: 12000000 INFO @ Tue, 14 Jul 2020 09:16:59: 8000000 INFO @ Tue, 14 Jul 2020 09:17:01: 3000000 INFO @ Tue, 14 Jul 2020 09:17:04: 13000000 INFO @ Tue, 14 Jul 2020 09:17:05: 9000000 INFO @ Tue, 14 Jul 2020 09:17:08: 4000000 INFO @ Tue, 14 Jul 2020 09:17:10: 14000000 INFO @ Tue, 14 Jul 2020 09:17:11: 10000000 INFO @ Tue, 14 Jul 2020 09:17:14: 5000000 INFO @ Tue, 14 Jul 2020 09:17:17: 15000000 INFO @ Tue, 14 Jul 2020 09:17:17: 11000000 INFO @ Tue, 14 Jul 2020 09:17:20: 6000000 INFO @ Tue, 14 Jul 2020 09:17:23: 12000000 INFO @ Tue, 14 Jul 2020 09:17:23: 16000000 INFO @ Tue, 14 Jul 2020 09:17:27: 7000000 INFO @ Tue, 14 Jul 2020 09:17:29: 13000000 INFO @ Tue, 14 Jul 2020 09:17:30: 17000000 INFO @ Tue, 14 Jul 2020 09:17:33: 8000000 INFO @ Tue, 14 Jul 2020 09:17:34: 14000000 INFO @ Tue, 14 Jul 2020 09:17:36: 18000000 INFO @ Tue, 14 Jul 2020 09:17:40: 9000000 INFO @ Tue, 14 Jul 2020 09:17:40: 15000000 INFO @ Tue, 14 Jul 2020 09:17:43: 19000000 INFO @ Tue, 14 Jul 2020 09:17:46: 16000000 INFO @ Tue, 14 Jul 2020 09:17:46: 10000000 INFO @ Tue, 14 Jul 2020 09:17:50: 20000000 INFO @ Tue, 14 Jul 2020 09:17:52: 17000000 INFO @ Tue, 14 Jul 2020 09:17:53: 11000000 INFO @ Tue, 14 Jul 2020 09:17:55: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:17:55: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:17:55: #1 total tags in treatment: 20752308 INFO @ Tue, 14 Jul 2020 09:17:55: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:17:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:17:55: #1 tags after filtering in treatment: 20752308 INFO @ Tue, 14 Jul 2020 09:17:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:17:55: #1 finished! INFO @ Tue, 14 Jul 2020 09:17:55: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:17:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:17:56: #2 number of paired peaks: 1 WARNING @ Tue, 14 Jul 2020 09:17:56: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:17:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:17:58: 18000000 INFO @ Tue, 14 Jul 2020 09:17:59: 12000000 INFO @ Tue, 14 Jul 2020 09:18:03: 19000000 INFO @ Tue, 14 Jul 2020 09:18:06: 13000000 INFO @ Tue, 14 Jul 2020 09:18:09: 20000000 INFO @ Tue, 14 Jul 2020 09:18:12: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:18:13: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:18:13: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:18:13: #1 total tags in treatment: 20752308 INFO @ Tue, 14 Jul 2020 09:18:13: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:18:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:18:14: #1 tags after filtering in treatment: 20752308 INFO @ Tue, 14 Jul 2020 09:18:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:18:14: #1 finished! INFO @ Tue, 14 Jul 2020 09:18:14: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:18:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:18:15: #2 number of paired peaks: 1 WARNING @ Tue, 14 Jul 2020 09:18:15: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:18:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:18:18: 15000000 INFO @ Tue, 14 Jul 2020 09:18:26: 16000000 INFO @ Tue, 14 Jul 2020 09:18:33: 17000000 INFO @ Tue, 14 Jul 2020 09:18:41: 18000000 INFO @ Tue, 14 Jul 2020 09:18:48: 19000000 INFO @ Tue, 14 Jul 2020 09:18:56: 20000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 09:19:02: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:19:02: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:19:02: #1 total tags in treatment: 20752308 INFO @ Tue, 14 Jul 2020 09:19:02: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:19:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:19:02: #1 tags after filtering in treatment: 20752308 INFO @ Tue, 14 Jul 2020 09:19:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:19:02: #1 finished! INFO @ Tue, 14 Jul 2020 09:19:02: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:19:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:19:03: #2 number of paired peaks: 1 WARNING @ Tue, 14 Jul 2020 09:19:03: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:19:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780057/SRX7780057.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling