Job ID = 6626917 SRX = SRX7780056 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-13T23:58:32 prefetch.2.10.7: 1) Downloading 'SRR11143811'... 2020-07-13T23:58:32 prefetch.2.10.7: Downloading via HTTPS... 2020-07-13T23:59:16 prefetch.2.10.7: HTTPS download succeed 2020-07-13T23:59:16 prefetch.2.10.7: 1) 'SRR11143811' was downloaded successfully Read 22791353 spots for SRR11143811/SRR11143811.sra Written 22791353 spots for SRR11143811/SRR11143811.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627090 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:48 22791353 reads; of these: 22791353 (100.00%) were unpaired; of these: 1851811 (8.13%) aligned 0 times 15460467 (67.83%) aligned exactly 1 time 5479075 (24.04%) aligned >1 times 91.87% overall alignment rate Time searching: 00:06:48 Overall time: 00:06:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3974968 / 20939542 = 0.1898 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:11:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:11:18: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:11:18: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:11:23: 1000000 INFO @ Tue, 14 Jul 2020 09:11:28: 2000000 INFO @ Tue, 14 Jul 2020 09:11:33: 3000000 INFO @ Tue, 14 Jul 2020 09:11:38: 4000000 INFO @ Tue, 14 Jul 2020 09:11:44: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:11:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:11:48: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:11:48: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:11:49: 6000000 INFO @ Tue, 14 Jul 2020 09:11:54: 1000000 INFO @ Tue, 14 Jul 2020 09:11:54: 7000000 INFO @ Tue, 14 Jul 2020 09:11:59: 2000000 INFO @ Tue, 14 Jul 2020 09:12:00: 8000000 INFO @ Tue, 14 Jul 2020 09:12:05: 3000000 INFO @ Tue, 14 Jul 2020 09:12:05: 9000000 INFO @ Tue, 14 Jul 2020 09:12:10: 4000000 INFO @ Tue, 14 Jul 2020 09:12:11: 10000000 INFO @ Tue, 14 Jul 2020 09:12:16: 5000000 INFO @ Tue, 14 Jul 2020 09:12:16: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:12:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:12:18: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:12:18: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:12:22: 6000000 INFO @ Tue, 14 Jul 2020 09:12:22: 12000000 INFO @ Tue, 14 Jul 2020 09:12:24: 1000000 INFO @ Tue, 14 Jul 2020 09:12:27: 13000000 INFO @ Tue, 14 Jul 2020 09:12:27: 7000000 INFO @ Tue, 14 Jul 2020 09:12:30: 2000000 INFO @ Tue, 14 Jul 2020 09:12:33: 14000000 INFO @ Tue, 14 Jul 2020 09:12:33: 8000000 INFO @ Tue, 14 Jul 2020 09:12:35: 3000000 INFO @ Tue, 14 Jul 2020 09:12:38: 15000000 INFO @ Tue, 14 Jul 2020 09:12:38: 9000000 INFO @ Tue, 14 Jul 2020 09:12:41: 4000000 INFO @ Tue, 14 Jul 2020 09:12:44: 16000000 INFO @ Tue, 14 Jul 2020 09:12:44: 10000000 INFO @ Tue, 14 Jul 2020 09:12:46: 5000000 INFO @ Tue, 14 Jul 2020 09:12:49: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:12:49: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:12:49: #1 total tags in treatment: 16964574 INFO @ Tue, 14 Jul 2020 09:12:49: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:12:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:12:49: #1 tags after filtering in treatment: 16964574 INFO @ Tue, 14 Jul 2020 09:12:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:12:49: #1 finished! INFO @ Tue, 14 Jul 2020 09:12:49: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:12:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:12:50: 11000000 INFO @ Tue, 14 Jul 2020 09:12:51: #2 number of paired peaks: 13 WARNING @ Tue, 14 Jul 2020 09:12:51: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:12:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:12:52: 6000000 INFO @ Tue, 14 Jul 2020 09:12:55: 12000000 INFO @ Tue, 14 Jul 2020 09:12:58: 7000000 INFO @ Tue, 14 Jul 2020 09:13:01: 13000000 INFO @ Tue, 14 Jul 2020 09:13:03: 8000000 INFO @ Tue, 14 Jul 2020 09:13:06: 14000000 INFO @ Tue, 14 Jul 2020 09:13:09: 9000000 INFO @ Tue, 14 Jul 2020 09:13:12: 15000000 INFO @ Tue, 14 Jul 2020 09:13:14: 10000000 INFO @ Tue, 14 Jul 2020 09:13:17: 16000000 INFO @ Tue, 14 Jul 2020 09:13:20: 11000000 INFO @ Tue, 14 Jul 2020 09:13:23: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:13:23: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:13:23: #1 total tags in treatment: 16964574 INFO @ Tue, 14 Jul 2020 09:13:23: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:13:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:13:23: #1 tags after filtering in treatment: 16964574 INFO @ Tue, 14 Jul 2020 09:13:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:13:23: #1 finished! INFO @ Tue, 14 Jul 2020 09:13:23: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:13:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:13:24: #2 number of paired peaks: 13 WARNING @ Tue, 14 Jul 2020 09:13:24: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:13:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:13:25: 12000000 INFO @ Tue, 14 Jul 2020 09:13:31: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:13:36: 14000000 INFO @ Tue, 14 Jul 2020 09:13:41: 15000000 INFO @ Tue, 14 Jul 2020 09:13:46: 16000000 INFO @ Tue, 14 Jul 2020 09:13:52: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:13:52: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:13:52: #1 total tags in treatment: 16964574 INFO @ Tue, 14 Jul 2020 09:13:52: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:13:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:13:52: #1 tags after filtering in treatment: 16964574 INFO @ Tue, 14 Jul 2020 09:13:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:13:52: #1 finished! INFO @ Tue, 14 Jul 2020 09:13:52: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:13:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:13:53: #2 number of paired peaks: 13 WARNING @ Tue, 14 Jul 2020 09:13:53: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:13:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780056/SRX7780056.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。