Job ID = 6626903 SRX = SRX7780052 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-13T23:57:02 prefetch.2.10.7: 1) Downloading 'SRR11143807'... 2020-07-13T23:57:02 prefetch.2.10.7: Downloading via HTTPS... 2020-07-13T23:59:26 prefetch.2.10.7: HTTPS download succeed 2020-07-13T23:59:26 prefetch.2.10.7: 1) 'SRR11143807' was downloaded successfully Read 24153088 spots for SRR11143807/SRR11143807.sra Written 24153088 spots for SRR11143807/SRR11143807.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627097 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:06 24153088 reads; of these: 24153088 (100.00%) were unpaired; of these: 2586540 (10.71%) aligned 0 times 15673318 (64.89%) aligned exactly 1 time 5893230 (24.40%) aligned >1 times 89.29% overall alignment rate Time searching: 00:07:06 Overall time: 00:07:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4218083 / 21566548 = 0.1956 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:12:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:12:10: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:12:10: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:12:16: 1000000 INFO @ Tue, 14 Jul 2020 09:12:21: 2000000 INFO @ Tue, 14 Jul 2020 09:12:26: 3000000 INFO @ Tue, 14 Jul 2020 09:12:32: 4000000 INFO @ Tue, 14 Jul 2020 09:12:37: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:12:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:12:40: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:12:40: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:12:42: 6000000 INFO @ Tue, 14 Jul 2020 09:12:46: 1000000 INFO @ Tue, 14 Jul 2020 09:12:47: 7000000 INFO @ Tue, 14 Jul 2020 09:12:52: 2000000 INFO @ Tue, 14 Jul 2020 09:12:53: 8000000 INFO @ Tue, 14 Jul 2020 09:12:57: 3000000 INFO @ Tue, 14 Jul 2020 09:12:58: 9000000 INFO @ Tue, 14 Jul 2020 09:13:03: 4000000 INFO @ Tue, 14 Jul 2020 09:13:03: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:13:08: 5000000 INFO @ Tue, 14 Jul 2020 09:13:08: 11000000 INFO @ Tue, 14 Jul 2020 09:13:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:13:10: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:13:10: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:13:14: 6000000 INFO @ Tue, 14 Jul 2020 09:13:14: 12000000 INFO @ Tue, 14 Jul 2020 09:13:17: 1000000 INFO @ Tue, 14 Jul 2020 09:13:20: 7000000 INFO @ Tue, 14 Jul 2020 09:13:20: 13000000 INFO @ Tue, 14 Jul 2020 09:13:24: 2000000 INFO @ Tue, 14 Jul 2020 09:13:25: 8000000 INFO @ Tue, 14 Jul 2020 09:13:25: 14000000 INFO @ Tue, 14 Jul 2020 09:13:31: 9000000 INFO @ Tue, 14 Jul 2020 09:13:31: 15000000 INFO @ Tue, 14 Jul 2020 09:13:32: 3000000 INFO @ Tue, 14 Jul 2020 09:13:37: 10000000 INFO @ Tue, 14 Jul 2020 09:13:37: 16000000 INFO @ Tue, 14 Jul 2020 09:13:40: 4000000 INFO @ Tue, 14 Jul 2020 09:13:43: 11000000 INFO @ Tue, 14 Jul 2020 09:13:43: 17000000 INFO @ Tue, 14 Jul 2020 09:13:45: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:13:45: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:13:45: #1 total tags in treatment: 17348465 INFO @ Tue, 14 Jul 2020 09:13:45: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:13:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:13:45: #1 tags after filtering in treatment: 17348465 INFO @ Tue, 14 Jul 2020 09:13:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:13:45: #1 finished! INFO @ Tue, 14 Jul 2020 09:13:45: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:13:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:13:46: #2 number of paired peaks: 13 WARNING @ Tue, 14 Jul 2020 09:13:46: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:13:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:13:47: 5000000 INFO @ Tue, 14 Jul 2020 09:13:48: 12000000 INFO @ Tue, 14 Jul 2020 09:13:54: 13000000 INFO @ Tue, 14 Jul 2020 09:13:55: 6000000 INFO @ Tue, 14 Jul 2020 09:13:59: 14000000 INFO @ Tue, 14 Jul 2020 09:14:03: 7000000 INFO @ Tue, 14 Jul 2020 09:14:05: 15000000 INFO @ Tue, 14 Jul 2020 09:14:10: 8000000 INFO @ Tue, 14 Jul 2020 09:14:10: 16000000 INFO @ Tue, 14 Jul 2020 09:14:16: 17000000 INFO @ Tue, 14 Jul 2020 09:14:18: 9000000 INFO @ Tue, 14 Jul 2020 09:14:18: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:14:18: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:14:18: #1 total tags in treatment: 17348465 INFO @ Tue, 14 Jul 2020 09:14:18: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:14:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:14:18: #1 tags after filtering in treatment: 17348465 INFO @ Tue, 14 Jul 2020 09:14:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:14:18: #1 finished! INFO @ Tue, 14 Jul 2020 09:14:18: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:14:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:14:19: #2 number of paired peaks: 13 WARNING @ Tue, 14 Jul 2020 09:14:19: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:14:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:14:25: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:14:32: 11000000 INFO @ Tue, 14 Jul 2020 09:14:39: 12000000 INFO @ Tue, 14 Jul 2020 09:14:46: 13000000 INFO @ Tue, 14 Jul 2020 09:14:53: 14000000 INFO @ Tue, 14 Jul 2020 09:15:00: 15000000 INFO @ Tue, 14 Jul 2020 09:15:08: 16000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 09:15:15: 17000000 INFO @ Tue, 14 Jul 2020 09:15:18: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:15:18: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:15:18: #1 total tags in treatment: 17348465 INFO @ Tue, 14 Jul 2020 09:15:18: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:15:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:15:18: #1 tags after filtering in treatment: 17348465 INFO @ Tue, 14 Jul 2020 09:15:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:15:18: #1 finished! INFO @ Tue, 14 Jul 2020 09:15:18: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:15:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:15:19: #2 number of paired peaks: 13 WARNING @ Tue, 14 Jul 2020 09:15:19: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:15:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780052/SRX7780052.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling