Job ID = 6626900 SRX = SRX7780051 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-13T23:56:02 prefetch.2.10.7: 1) Downloading 'SRR11143806'... 2020-07-13T23:56:02 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T00:01:27 prefetch.2.10.7: HTTPS download failed 2020-07-14T00:01:27 prefetch.2.10.7: 1) failed to download SRR11143806 2020-07-14T00:01:36 prefetch.2.10.7: 1) Downloading 'SRR11143806'... 2020-07-14T00:01:36 prefetch.2.10.7: Downloading via HTTPS... 2020-07-14T00:01:36 prefetch.2.10.7: Continue download of 'SRR11143806' from 728776212 2020-07-14T00:01:50 prefetch.2.10.7: HTTPS download succeed 2020-07-14T00:01:50 prefetch.2.10.7: 1) 'SRR11143806' was downloaded successfully Read 27238835 spots for SRR11143806/SRR11143806.sra Written 27238835 spots for SRR11143806/SRR11143806.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627105 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:03 27238835 reads; of these: 27238835 (100.00%) were unpaired; of these: 2545611 (9.35%) aligned 0 times 18724234 (68.74%) aligned exactly 1 time 5968990 (21.91%) aligned >1 times 90.65% overall alignment rate Time searching: 00:07:03 Overall time: 00:07:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6353618 / 24693224 = 0.2573 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:15:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:15:16: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:15:16: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:15:22: 1000000 INFO @ Tue, 14 Jul 2020 09:15:27: 2000000 INFO @ Tue, 14 Jul 2020 09:15:32: 3000000 INFO @ Tue, 14 Jul 2020 09:15:38: 4000000 INFO @ Tue, 14 Jul 2020 09:15:43: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:15:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:15:46: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:15:46: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:15:48: 6000000 INFO @ Tue, 14 Jul 2020 09:15:52: 1000000 INFO @ Tue, 14 Jul 2020 09:15:54: 7000000 INFO @ Tue, 14 Jul 2020 09:15:58: 2000000 INFO @ Tue, 14 Jul 2020 09:16:00: 8000000 INFO @ Tue, 14 Jul 2020 09:16:03: 3000000 INFO @ Tue, 14 Jul 2020 09:16:05: 9000000 INFO @ Tue, 14 Jul 2020 09:16:09: 4000000 INFO @ Tue, 14 Jul 2020 09:16:11: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:16:15: 5000000 INFO @ Tue, 14 Jul 2020 09:16:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:16:16: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:16:16: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:16:17: 11000000 INFO @ Tue, 14 Jul 2020 09:16:20: 6000000 INFO @ Tue, 14 Jul 2020 09:16:22: 1000000 INFO @ Tue, 14 Jul 2020 09:16:22: 12000000 INFO @ Tue, 14 Jul 2020 09:16:26: 7000000 INFO @ Tue, 14 Jul 2020 09:16:27: 2000000 INFO @ Tue, 14 Jul 2020 09:16:28: 13000000 INFO @ Tue, 14 Jul 2020 09:16:32: 8000000 INFO @ Tue, 14 Jul 2020 09:16:33: 3000000 INFO @ Tue, 14 Jul 2020 09:16:34: 14000000 INFO @ Tue, 14 Jul 2020 09:16:37: 9000000 INFO @ Tue, 14 Jul 2020 09:16:39: 4000000 INFO @ Tue, 14 Jul 2020 09:16:40: 15000000 INFO @ Tue, 14 Jul 2020 09:16:43: 10000000 INFO @ Tue, 14 Jul 2020 09:16:44: 5000000 INFO @ Tue, 14 Jul 2020 09:16:45: 16000000 INFO @ Tue, 14 Jul 2020 09:16:49: 11000000 INFO @ Tue, 14 Jul 2020 09:16:50: 6000000 INFO @ Tue, 14 Jul 2020 09:16:51: 17000000 INFO @ Tue, 14 Jul 2020 09:16:54: 12000000 INFO @ Tue, 14 Jul 2020 09:16:56: 7000000 INFO @ Tue, 14 Jul 2020 09:16:57: 18000000 INFO @ Tue, 14 Jul 2020 09:16:59: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:16:59: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:16:59: #1 total tags in treatment: 18339606 INFO @ Tue, 14 Jul 2020 09:16:59: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:16:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:16:59: #1 tags after filtering in treatment: 18339606 INFO @ Tue, 14 Jul 2020 09:16:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:16:59: #1 finished! INFO @ Tue, 14 Jul 2020 09:16:59: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:16:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:17:00: #2 number of paired peaks: 10 WARNING @ Tue, 14 Jul 2020 09:17:00: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:17:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:17:00: 13000000 INFO @ Tue, 14 Jul 2020 09:17:01: 8000000 INFO @ Tue, 14 Jul 2020 09:17:06: 14000000 INFO @ Tue, 14 Jul 2020 09:17:07: 9000000 INFO @ Tue, 14 Jul 2020 09:17:12: 15000000 INFO @ Tue, 14 Jul 2020 09:17:12: 10000000 INFO @ Tue, 14 Jul 2020 09:17:18: 16000000 INFO @ Tue, 14 Jul 2020 09:17:18: 11000000 INFO @ Tue, 14 Jul 2020 09:17:23: 12000000 INFO @ Tue, 14 Jul 2020 09:17:23: 17000000 INFO @ Tue, 14 Jul 2020 09:17:29: 13000000 INFO @ Tue, 14 Jul 2020 09:17:29: 18000000 INFO @ Tue, 14 Jul 2020 09:17:31: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:17:31: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:17:31: #1 total tags in treatment: 18339606 INFO @ Tue, 14 Jul 2020 09:17:31: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:17:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:17:32: #1 tags after filtering in treatment: 18339606 INFO @ Tue, 14 Jul 2020 09:17:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:17:32: #1 finished! INFO @ Tue, 14 Jul 2020 09:17:32: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:17:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:17:33: #2 number of paired peaks: 10 WARNING @ Tue, 14 Jul 2020 09:17:33: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:17:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:17:35: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:17:40: 15000000 INFO @ Tue, 14 Jul 2020 09:17:45: 16000000 INFO @ Tue, 14 Jul 2020 09:17:51: 17000000 INFO @ Tue, 14 Jul 2020 09:17:56: 18000000 INFO @ Tue, 14 Jul 2020 09:17:58: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:17:58: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:17:58: #1 total tags in treatment: 18339606 INFO @ Tue, 14 Jul 2020 09:17:58: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:17:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:17:58: #1 tags after filtering in treatment: 18339606 INFO @ Tue, 14 Jul 2020 09:17:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:17:58: #1 finished! INFO @ Tue, 14 Jul 2020 09:17:58: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:17:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:18:00: #2 number of paired peaks: 10 WARNING @ Tue, 14 Jul 2020 09:18:00: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:18:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780051/SRX7780051.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。