Job ID = 6626894 SRX = SRX7780050 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-13T23:56:05 prefetch.2.10.7: 1) Downloading 'SRR11143805'... 2020-07-13T23:56:05 prefetch.2.10.7: Downloading via HTTPS... 2020-07-13T23:59:27 prefetch.2.10.7: HTTPS download succeed 2020-07-13T23:59:28 prefetch.2.10.7: 'SRR11143805' is valid 2020-07-13T23:59:28 prefetch.2.10.7: 1) 'SRR11143805' was downloaded successfully Read 13531138 spots for SRR11143805/SRR11143805.sra Written 13531138 spots for SRR11143805/SRR11143805.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627084 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:47 13531138 reads; of these: 13531138 (100.00%) were unpaired; of these: 1480439 (10.94%) aligned 0 times 8673996 (64.10%) aligned exactly 1 time 3376703 (24.96%) aligned >1 times 89.06% overall alignment rate Time searching: 00:03:47 Overall time: 00:03:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1701421 / 12050699 = 0.1412 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:06:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:06:49: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:06:49: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:06:56: 1000000 INFO @ Tue, 14 Jul 2020 09:07:02: 2000000 INFO @ Tue, 14 Jul 2020 09:07:08: 3000000 INFO @ Tue, 14 Jul 2020 09:07:14: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:07:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:07:19: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:07:19: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:07:20: 5000000 INFO @ Tue, 14 Jul 2020 09:07:25: 1000000 INFO @ Tue, 14 Jul 2020 09:07:27: 6000000 INFO @ Tue, 14 Jul 2020 09:07:31: 2000000 INFO @ Tue, 14 Jul 2020 09:07:33: 7000000 INFO @ Tue, 14 Jul 2020 09:07:37: 3000000 INFO @ Tue, 14 Jul 2020 09:07:40: 8000000 INFO @ Tue, 14 Jul 2020 09:07:42: 4000000 INFO @ Tue, 14 Jul 2020 09:07:46: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:07:48: 5000000 INFO @ Tue, 14 Jul 2020 09:07:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:07:49: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:07:49: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:07:53: 10000000 INFO @ Tue, 14 Jul 2020 09:07:54: 6000000 INFO @ Tue, 14 Jul 2020 09:07:55: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:07:55: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:07:55: #1 total tags in treatment: 10349278 INFO @ Tue, 14 Jul 2020 09:07:55: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:07:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:07:55: #1 tags after filtering in treatment: 10349278 INFO @ Tue, 14 Jul 2020 09:07:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:07:55: #1 finished! INFO @ Tue, 14 Jul 2020 09:07:55: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:07:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:07:55: 1000000 INFO @ Tue, 14 Jul 2020 09:07:56: #2 number of paired peaks: 88 WARNING @ Tue, 14 Jul 2020 09:07:56: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:07:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:07:59: 7000000 INFO @ Tue, 14 Jul 2020 09:08:01: 2000000 INFO @ Tue, 14 Jul 2020 09:08:05: 8000000 INFO @ Tue, 14 Jul 2020 09:08:06: 3000000 INFO @ Tue, 14 Jul 2020 09:08:11: 9000000 INFO @ Tue, 14 Jul 2020 09:08:12: 4000000 INFO @ Tue, 14 Jul 2020 09:08:16: 10000000 INFO @ Tue, 14 Jul 2020 09:08:18: 5000000 INFO @ Tue, 14 Jul 2020 09:08:18: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:08:18: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:08:18: #1 total tags in treatment: 10349278 INFO @ Tue, 14 Jul 2020 09:08:18: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:08:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:08:18: #1 tags after filtering in treatment: 10349278 INFO @ Tue, 14 Jul 2020 09:08:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:08:18: #1 finished! INFO @ Tue, 14 Jul 2020 09:08:18: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:08:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:08:19: #2 number of paired peaks: 88 WARNING @ Tue, 14 Jul 2020 09:08:19: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:08:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:08:23: 6000000 INFO @ Tue, 14 Jul 2020 09:08:28: 7000000 INFO @ Tue, 14 Jul 2020 09:08:34: 8000000 INFO @ Tue, 14 Jul 2020 09:08:39: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:08:44: 10000000 INFO @ Tue, 14 Jul 2020 09:08:46: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:08:46: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:08:46: #1 total tags in treatment: 10349278 INFO @ Tue, 14 Jul 2020 09:08:46: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:08:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:08:46: #1 tags after filtering in treatment: 10349278 INFO @ Tue, 14 Jul 2020 09:08:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:08:46: #1 finished! INFO @ Tue, 14 Jul 2020 09:08:46: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:08:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:08:47: #2 number of paired peaks: 88 WARNING @ Tue, 14 Jul 2020 09:08:47: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:08:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780050/SRX7780050.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。