Job ID = 6626892 SRX = SRX7780049 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-13T23:57:31 prefetch.2.10.7: 1) Downloading 'SRR11143804'... 2020-07-13T23:57:31 prefetch.2.10.7: Downloading via HTTPS... 2020-07-13T23:58:51 prefetch.2.10.7: HTTPS download succeed 2020-07-13T23:58:51 prefetch.2.10.7: 1) 'SRR11143804' was downloaded successfully Read 26808455 spots for SRR11143804/SRR11143804.sra Written 26808455 spots for SRR11143804/SRR11143804.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627098 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:34 26808455 reads; of these: 26808455 (100.00%) were unpaired; of these: 3822768 (14.26%) aligned 0 times 16865044 (62.91%) aligned exactly 1 time 6120643 (22.83%) aligned >1 times 85.74% overall alignment rate Time searching: 00:07:34 Overall time: 00:07:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5466022 / 22985687 = 0.2378 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:12:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:12:28: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:12:28: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:12:33: 1000000 INFO @ Tue, 14 Jul 2020 09:12:39: 2000000 INFO @ Tue, 14 Jul 2020 09:12:44: 3000000 INFO @ Tue, 14 Jul 2020 09:12:50: 4000000 INFO @ Tue, 14 Jul 2020 09:12:55: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:12:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:12:58: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:12:58: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:13:01: 6000000 INFO @ Tue, 14 Jul 2020 09:13:04: 1000000 INFO @ Tue, 14 Jul 2020 09:13:06: 7000000 INFO @ Tue, 14 Jul 2020 09:13:09: 2000000 INFO @ Tue, 14 Jul 2020 09:13:12: 8000000 INFO @ Tue, 14 Jul 2020 09:13:14: 3000000 INFO @ Tue, 14 Jul 2020 09:13:17: 9000000 INFO @ Tue, 14 Jul 2020 09:13:19: 4000000 INFO @ Tue, 14 Jul 2020 09:13:23: 10000000 INFO @ Tue, 14 Jul 2020 09:13:24: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 09:13:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 09:13:28: #1 read tag files... INFO @ Tue, 14 Jul 2020 09:13:28: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 09:13:29: 6000000 INFO @ Tue, 14 Jul 2020 09:13:29: 11000000 INFO @ Tue, 14 Jul 2020 09:13:34: 7000000 INFO @ Tue, 14 Jul 2020 09:13:34: 1000000 INFO @ Tue, 14 Jul 2020 09:13:34: 12000000 INFO @ Tue, 14 Jul 2020 09:13:39: 8000000 INFO @ Tue, 14 Jul 2020 09:13:39: 2000000 INFO @ Tue, 14 Jul 2020 09:13:40: 13000000 INFO @ Tue, 14 Jul 2020 09:13:43: 9000000 INFO @ Tue, 14 Jul 2020 09:13:44: 3000000 INFO @ Tue, 14 Jul 2020 09:13:45: 14000000 INFO @ Tue, 14 Jul 2020 09:13:48: 10000000 INFO @ Tue, 14 Jul 2020 09:13:49: 4000000 INFO @ Tue, 14 Jul 2020 09:13:51: 15000000 INFO @ Tue, 14 Jul 2020 09:13:53: 11000000 INFO @ Tue, 14 Jul 2020 09:13:54: 5000000 INFO @ Tue, 14 Jul 2020 09:13:57: 16000000 INFO @ Tue, 14 Jul 2020 09:13:58: 12000000 INFO @ Tue, 14 Jul 2020 09:13:59: 6000000 INFO @ Tue, 14 Jul 2020 09:14:03: 17000000 INFO @ Tue, 14 Jul 2020 09:14:03: 13000000 INFO @ Tue, 14 Jul 2020 09:14:04: 7000000 INFO @ Tue, 14 Jul 2020 09:14:06: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:14:06: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:14:06: #1 total tags in treatment: 17519665 INFO @ Tue, 14 Jul 2020 09:14:06: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:14:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:14:06: #1 tags after filtering in treatment: 17519665 INFO @ Tue, 14 Jul 2020 09:14:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:14:06: #1 finished! INFO @ Tue, 14 Jul 2020 09:14:06: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:14:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:14:07: #2 number of paired peaks: 59 WARNING @ Tue, 14 Jul 2020 09:14:07: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:14:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:14:08: 14000000 INFO @ Tue, 14 Jul 2020 09:14:09: 8000000 INFO @ Tue, 14 Jul 2020 09:14:13: 15000000 INFO @ Tue, 14 Jul 2020 09:14:14: 9000000 INFO @ Tue, 14 Jul 2020 09:14:18: 16000000 INFO @ Tue, 14 Jul 2020 09:14:19: 10000000 INFO @ Tue, 14 Jul 2020 09:14:23: 17000000 INFO @ Tue, 14 Jul 2020 09:14:24: 11000000 INFO @ Tue, 14 Jul 2020 09:14:25: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:14:25: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:14:25: #1 total tags in treatment: 17519665 INFO @ Tue, 14 Jul 2020 09:14:25: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:14:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:14:26: #1 tags after filtering in treatment: 17519665 INFO @ Tue, 14 Jul 2020 09:14:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:14:26: #1 finished! INFO @ Tue, 14 Jul 2020 09:14:26: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:14:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:14:27: #2 number of paired peaks: 59 WARNING @ Tue, 14 Jul 2020 09:14:27: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:14:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:14:28: 12000000 INFO @ Tue, 14 Jul 2020 09:14:33: 13000000 INFO @ Tue, 14 Jul 2020 09:14:38: 14000000 INFO @ Tue, 14 Jul 2020 09:14:43: 15000000 INFO @ Tue, 14 Jul 2020 09:14:47: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:14:52: 17000000 INFO @ Tue, 14 Jul 2020 09:14:55: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:14:55: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:14:55: #1 total tags in treatment: 17519665 INFO @ Tue, 14 Jul 2020 09:14:55: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:14:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:14:55: #1 tags after filtering in treatment: 17519665 INFO @ Tue, 14 Jul 2020 09:14:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:14:55: #1 finished! INFO @ Tue, 14 Jul 2020 09:14:55: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:14:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:14:56: #2 number of paired peaks: 59 WARNING @ Tue, 14 Jul 2020 09:14:56: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:14:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780049/SRX7780049.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。