Job ID = 6626784 SRX = SRX7780039 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-07-13T23:46:21 prefetch.2.10.7: 1) Downloading 'SRR11143794'... 2020-07-13T23:46:21 prefetch.2.10.7: Downloading via HTTPS... 2020-07-13T23:47:31 prefetch.2.10.7: HTTPS download succeed 2020-07-13T23:47:31 prefetch.2.10.7: 1) 'SRR11143794' was downloaded successfully Read 20605928 spots for SRR11143794/SRR11143794.sra Written 20605928 spots for SRR11143794/SRR11143794.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627049 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:34 20605928 reads; of these: 20605928 (100.00%) were unpaired; of these: 1510611 (7.33%) aligned 0 times 15671195 (76.05%) aligned exactly 1 time 3424122 (16.62%) aligned >1 times 92.67% overall alignment rate Time searching: 00:05:34 Overall time: 00:05:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4155468 / 19095317 = 0.2176 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:58:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:58:32: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:58:32: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:58:40: 1000000 INFO @ Tue, 14 Jul 2020 08:58:48: 2000000 INFO @ Tue, 14 Jul 2020 08:58:57: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:59:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:59:02: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:59:02: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:59:05: 4000000 INFO @ Tue, 14 Jul 2020 08:59:08: 1000000 INFO @ Tue, 14 Jul 2020 08:59:14: 5000000 INFO @ Tue, 14 Jul 2020 08:59:15: 2000000 INFO @ Tue, 14 Jul 2020 08:59:22: 3000000 INFO @ Tue, 14 Jul 2020 08:59:23: 6000000 INFO @ Tue, 14 Jul 2020 08:59:29: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:59:32: 7000000 INFO @ Tue, 14 Jul 2020 08:59:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:59:32: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:59:32: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:59:36: 5000000 INFO @ Tue, 14 Jul 2020 08:59:40: 8000000 INFO @ Tue, 14 Jul 2020 08:59:41: 1000000 INFO @ Tue, 14 Jul 2020 08:59:43: 6000000 INFO @ Tue, 14 Jul 2020 08:59:49: 9000000 INFO @ Tue, 14 Jul 2020 08:59:50: 2000000 INFO @ Tue, 14 Jul 2020 08:59:50: 7000000 INFO @ Tue, 14 Jul 2020 08:59:57: 8000000 INFO @ Tue, 14 Jul 2020 08:59:58: 10000000 INFO @ Tue, 14 Jul 2020 08:59:59: 3000000 INFO @ Tue, 14 Jul 2020 09:00:04: 9000000 INFO @ Tue, 14 Jul 2020 09:00:07: 11000000 INFO @ Tue, 14 Jul 2020 09:00:08: 4000000 INFO @ Tue, 14 Jul 2020 09:00:11: 10000000 INFO @ Tue, 14 Jul 2020 09:00:15: 12000000 INFO @ Tue, 14 Jul 2020 09:00:17: 5000000 INFO @ Tue, 14 Jul 2020 09:00:18: 11000000 INFO @ Tue, 14 Jul 2020 09:00:24: 13000000 INFO @ Tue, 14 Jul 2020 09:00:26: 12000000 INFO @ Tue, 14 Jul 2020 09:00:26: 6000000 INFO @ Tue, 14 Jul 2020 09:00:33: 13000000 INFO @ Tue, 14 Jul 2020 09:00:33: 14000000 INFO @ Tue, 14 Jul 2020 09:00:35: 7000000 INFO @ Tue, 14 Jul 2020 09:00:40: 14000000 INFO @ Tue, 14 Jul 2020 09:00:42: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:00:42: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:00:42: #1 total tags in treatment: 14939849 INFO @ Tue, 14 Jul 2020 09:00:42: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:00:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:00:42: #1 tags after filtering in treatment: 14939849 INFO @ Tue, 14 Jul 2020 09:00:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:00:42: #1 finished! INFO @ Tue, 14 Jul 2020 09:00:42: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:00:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:00:43: #2 number of paired peaks: 21 WARNING @ Tue, 14 Jul 2020 09:00:43: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:00:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 09:00:44: 8000000 INFO @ Tue, 14 Jul 2020 09:00:47: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:00:47: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:00:47: #1 total tags in treatment: 14939849 INFO @ Tue, 14 Jul 2020 09:00:47: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:00:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:00:47: #1 tags after filtering in treatment: 14939849 INFO @ Tue, 14 Jul 2020 09:00:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:00:47: #1 finished! INFO @ Tue, 14 Jul 2020 09:00:47: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:00:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:00:48: #2 number of paired peaks: 21 WARNING @ Tue, 14 Jul 2020 09:00:48: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:00:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 09:00:53: 9000000 INFO @ Tue, 14 Jul 2020 09:01:03: 10000000 INFO @ Tue, 14 Jul 2020 09:01:12: 11000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 09:01:21: 12000000 INFO @ Tue, 14 Jul 2020 09:01:31: 13000000 INFO @ Tue, 14 Jul 2020 09:01:40: 14000000 INFO @ Tue, 14 Jul 2020 09:01:48: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 09:01:48: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 09:01:48: #1 total tags in treatment: 14939849 INFO @ Tue, 14 Jul 2020 09:01:48: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 09:01:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 09:01:48: #1 tags after filtering in treatment: 14939849 INFO @ Tue, 14 Jul 2020 09:01:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 09:01:48: #1 finished! INFO @ Tue, 14 Jul 2020 09:01:48: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 09:01:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 09:01:49: #2 number of paired peaks: 21 WARNING @ Tue, 14 Jul 2020 09:01:49: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 09:01:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780039/SRX7780039.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling