Job ID = 6626773 SRX = SRX7780033 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 17731747 spots for SRR11143788/SRR11143788.sra Written 17731747 spots for SRR11143788/SRR11143788.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627037 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:31 17731747 reads; of these: 17731747 (100.00%) were unpaired; of these: 2408093 (13.58%) aligned 0 times 12150475 (68.52%) aligned exactly 1 time 3173179 (17.90%) aligned >1 times 86.42% overall alignment rate Time searching: 00:04:31 Overall time: 00:04:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2666509 / 15323654 = 0.1740 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:51:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:51:10: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:51:10: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:51:16: 1000000 INFO @ Tue, 14 Jul 2020 08:51:21: 2000000 INFO @ Tue, 14 Jul 2020 08:51:26: 3000000 INFO @ Tue, 14 Jul 2020 08:51:32: 4000000 INFO @ Tue, 14 Jul 2020 08:51:37: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:51:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:51:40: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:51:40: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:51:43: 6000000 INFO @ Tue, 14 Jul 2020 08:51:46: 1000000 INFO @ Tue, 14 Jul 2020 08:51:49: 7000000 INFO @ Tue, 14 Jul 2020 08:51:51: 2000000 INFO @ Tue, 14 Jul 2020 08:51:54: 8000000 INFO @ Tue, 14 Jul 2020 08:51:57: 3000000 INFO @ Tue, 14 Jul 2020 08:52:00: 9000000 INFO @ Tue, 14 Jul 2020 08:52:02: 4000000 INFO @ Tue, 14 Jul 2020 08:52:05: 10000000 INFO @ Tue, 14 Jul 2020 08:52:08: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:52:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:52:10: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:52:10: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:52:11: 11000000 INFO @ Tue, 14 Jul 2020 08:52:14: 6000000 INFO @ Tue, 14 Jul 2020 08:52:16: 1000000 INFO @ Tue, 14 Jul 2020 08:52:16: 12000000 INFO @ Tue, 14 Jul 2020 08:52:20: 7000000 INFO @ Tue, 14 Jul 2020 08:52:20: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:52:20: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:52:20: #1 total tags in treatment: 12657145 INFO @ Tue, 14 Jul 2020 08:52:20: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:52:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:52:20: #1 tags after filtering in treatment: 12657145 INFO @ Tue, 14 Jul 2020 08:52:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:52:20: #1 finished! INFO @ Tue, 14 Jul 2020 08:52:20: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:52:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:52:21: #2 number of paired peaks: 28 WARNING @ Tue, 14 Jul 2020 08:52:21: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:52:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:52:21: 2000000 INFO @ Tue, 14 Jul 2020 08:52:25: 8000000 INFO @ Tue, 14 Jul 2020 08:52:27: 3000000 INFO @ Tue, 14 Jul 2020 08:52:31: 9000000 INFO @ Tue, 14 Jul 2020 08:52:32: 4000000 INFO @ Tue, 14 Jul 2020 08:52:36: 10000000 INFO @ Tue, 14 Jul 2020 08:52:38: 5000000 INFO @ Tue, 14 Jul 2020 08:52:42: 11000000 INFO @ Tue, 14 Jul 2020 08:52:44: 6000000 INFO @ Tue, 14 Jul 2020 08:52:47: 12000000 INFO @ Tue, 14 Jul 2020 08:52:49: 7000000 INFO @ Tue, 14 Jul 2020 08:52:51: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:52:51: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:52:51: #1 total tags in treatment: 12657145 INFO @ Tue, 14 Jul 2020 08:52:51: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:52:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:52:51: #1 tags after filtering in treatment: 12657145 INFO @ Tue, 14 Jul 2020 08:52:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:52:51: #1 finished! INFO @ Tue, 14 Jul 2020 08:52:51: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:52:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:52:52: #2 number of paired peaks: 28 WARNING @ Tue, 14 Jul 2020 08:52:52: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:52:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:52:55: 8000000 INFO @ Tue, 14 Jul 2020 08:53:01: 9000000 INFO @ Tue, 14 Jul 2020 08:53:06: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:53:12: 11000000 INFO @ Tue, 14 Jul 2020 08:53:17: 12000000 INFO @ Tue, 14 Jul 2020 08:53:21: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:53:21: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:53:21: #1 total tags in treatment: 12657145 INFO @ Tue, 14 Jul 2020 08:53:21: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:53:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:53:21: #1 tags after filtering in treatment: 12657145 INFO @ Tue, 14 Jul 2020 08:53:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:53:21: #1 finished! INFO @ Tue, 14 Jul 2020 08:53:21: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:53:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:53:22: #2 number of paired peaks: 28 WARNING @ Tue, 14 Jul 2020 08:53:22: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:53:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780033/SRX7780033.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。