Job ID = 6626770 SRX = SRX7780030 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 22205307 spots for SRR11143785/SRR11143785.sra Written 22205307 spots for SRR11143785/SRR11143785.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627040 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:30 22205307 reads; of these: 22205307 (100.00%) were unpaired; of these: 1708260 (7.69%) aligned 0 times 14950878 (67.33%) aligned exactly 1 time 5546169 (24.98%) aligned >1 times 92.31% overall alignment rate Time searching: 00:06:30 Overall time: 00:06:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3932648 / 20497047 = 0.1919 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:52:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:52:31: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:52:31: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:52:36: 1000000 INFO @ Tue, 14 Jul 2020 08:52:42: 2000000 INFO @ Tue, 14 Jul 2020 08:52:48: 3000000 INFO @ Tue, 14 Jul 2020 08:52:53: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:52:59: 5000000 INFO @ Tue, 14 Jul 2020 08:53:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:53:00: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:53:00: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:53:05: 6000000 INFO @ Tue, 14 Jul 2020 08:53:07: 1000000 INFO @ Tue, 14 Jul 2020 08:53:11: 7000000 INFO @ Tue, 14 Jul 2020 08:53:13: 2000000 INFO @ Tue, 14 Jul 2020 08:53:17: 8000000 INFO @ Tue, 14 Jul 2020 08:53:19: 3000000 INFO @ Tue, 14 Jul 2020 08:53:23: 9000000 INFO @ Tue, 14 Jul 2020 08:53:25: 4000000 INFO @ Tue, 14 Jul 2020 08:53:29: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:53:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:53:31: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:53:31: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:53:31: 5000000 INFO @ Tue, 14 Jul 2020 08:53:35: 11000000 INFO @ Tue, 14 Jul 2020 08:53:37: 6000000 INFO @ Tue, 14 Jul 2020 08:53:37: 1000000 INFO @ Tue, 14 Jul 2020 08:53:41: 12000000 INFO @ Tue, 14 Jul 2020 08:53:43: 7000000 INFO @ Tue, 14 Jul 2020 08:53:45: 2000000 INFO @ Tue, 14 Jul 2020 08:53:47: 13000000 INFO @ Tue, 14 Jul 2020 08:53:49: 8000000 INFO @ Tue, 14 Jul 2020 08:53:52: 3000000 INFO @ Tue, 14 Jul 2020 08:53:53: 14000000 INFO @ Tue, 14 Jul 2020 08:53:55: 9000000 INFO @ Tue, 14 Jul 2020 08:53:58: 4000000 INFO @ Tue, 14 Jul 2020 08:53:59: 15000000 INFO @ Tue, 14 Jul 2020 08:54:01: 10000000 INFO @ Tue, 14 Jul 2020 08:54:04: 5000000 INFO @ Tue, 14 Jul 2020 08:54:05: 16000000 INFO @ Tue, 14 Jul 2020 08:54:07: 11000000 INFO @ Tue, 14 Jul 2020 08:54:09: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:54:09: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:54:09: #1 total tags in treatment: 16564399 INFO @ Tue, 14 Jul 2020 08:54:09: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:54:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:54:09: #1 tags after filtering in treatment: 16564399 INFO @ Tue, 14 Jul 2020 08:54:09: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:54:09: #1 finished! INFO @ Tue, 14 Jul 2020 08:54:09: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:54:09: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:54:10: #2 number of paired peaks: 13 WARNING @ Tue, 14 Jul 2020 08:54:10: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:54:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:54:10: 6000000 INFO @ Tue, 14 Jul 2020 08:54:13: 12000000 INFO @ Tue, 14 Jul 2020 08:54:17: 7000000 INFO @ Tue, 14 Jul 2020 08:54:19: 13000000 INFO @ Tue, 14 Jul 2020 08:54:23: 8000000 INFO @ Tue, 14 Jul 2020 08:54:25: 14000000 INFO @ Tue, 14 Jul 2020 08:54:29: 9000000 INFO @ Tue, 14 Jul 2020 08:54:31: 15000000 INFO @ Tue, 14 Jul 2020 08:54:35: 10000000 INFO @ Tue, 14 Jul 2020 08:54:37: 16000000 INFO @ Tue, 14 Jul 2020 08:54:40: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:54:40: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:54:40: #1 total tags in treatment: 16564399 INFO @ Tue, 14 Jul 2020 08:54:40: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:54:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:54:41: #1 tags after filtering in treatment: 16564399 INFO @ Tue, 14 Jul 2020 08:54:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:54:41: #1 finished! INFO @ Tue, 14 Jul 2020 08:54:41: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:54:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:54:41: 11000000 INFO @ Tue, 14 Jul 2020 08:54:42: #2 number of paired peaks: 13 WARNING @ Tue, 14 Jul 2020 08:54:42: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:54:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:54:47: 12000000 INFO @ Tue, 14 Jul 2020 08:54:53: 13000000 INFO @ Tue, 14 Jul 2020 08:54:59: 14000000 INFO @ Tue, 14 Jul 2020 08:55:05: 15000000 INFO @ Tue, 14 Jul 2020 08:55:11: 16000000 INFO @ Tue, 14 Jul 2020 08:55:14: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:55:14: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:55:14: #1 total tags in treatment: 16564399 INFO @ Tue, 14 Jul 2020 08:55:14: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:55:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:55:15: #1 tags after filtering in treatment: 16564399 INFO @ Tue, 14 Jul 2020 08:55:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:55:15: #1 finished! INFO @ Tue, 14 Jul 2020 08:55:15: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:55:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:55:16: #2 number of paired peaks: 13 WARNING @ Tue, 14 Jul 2020 08:55:16: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:55:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7780030/SRX7780030.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。