Job ID = 14166986 SRX = SRX7723719 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 25811605 spots for SRR11084675/SRR11084675.sra Written 25811605 spots for SRR11084675/SRR11084675.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167344 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:05 25811605 reads; of these: 25811605 (100.00%) were unpaired; of these: 9262418 (35.88%) aligned 0 times 12209555 (47.30%) aligned exactly 1 time 4339632 (16.81%) aligned >1 times 64.12% overall alignment rate Time searching: 00:11:05 Overall time: 00:11:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5275561 / 16549187 = 0.3188 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:45:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:45:29: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:45:29: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:45:38: 1000000 INFO @ Fri, 10 Dec 2021 08:45:47: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:45:56: 3000000 INFO @ Fri, 10 Dec 2021 08:45:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:45:57: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:45:57: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:46:06: 4000000 INFO @ Fri, 10 Dec 2021 08:46:09: 1000000 INFO @ Fri, 10 Dec 2021 08:46:14: 5000000 INFO @ Fri, 10 Dec 2021 08:46:21: 2000000 INFO @ Fri, 10 Dec 2021 08:46:23: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:46:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:46:27: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:46:27: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:46:32: 7000000 INFO @ Fri, 10 Dec 2021 08:46:34: 3000000 INFO @ Fri, 10 Dec 2021 08:46:41: 1000000 INFO @ Fri, 10 Dec 2021 08:46:42: 8000000 INFO @ Fri, 10 Dec 2021 08:46:47: 4000000 INFO @ Fri, 10 Dec 2021 08:46:51: 9000000 INFO @ Fri, 10 Dec 2021 08:46:53: 2000000 INFO @ Fri, 10 Dec 2021 08:47:01: 10000000 INFO @ Fri, 10 Dec 2021 08:47:01: 5000000 INFO @ Fri, 10 Dec 2021 08:47:07: 3000000 INFO @ Fri, 10 Dec 2021 08:47:09: 11000000 INFO @ Fri, 10 Dec 2021 08:47:12: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:47:12: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:47:12: #1 total tags in treatment: 11273626 INFO @ Fri, 10 Dec 2021 08:47:12: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:47:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:47:12: #1 tags after filtering in treatment: 11273626 INFO @ Fri, 10 Dec 2021 08:47:12: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:47:12: #1 finished! INFO @ Fri, 10 Dec 2021 08:47:12: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:47:12: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:47:13: #2 number of paired peaks: 28 WARNING @ Fri, 10 Dec 2021 08:47:13: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:47:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:47:14: 6000000 INFO @ Fri, 10 Dec 2021 08:47:21: 4000000 INFO @ Fri, 10 Dec 2021 08:47:28: 7000000 INFO @ Fri, 10 Dec 2021 08:47:35: 5000000 INFO @ Fri, 10 Dec 2021 08:47:41: 8000000 INFO @ Fri, 10 Dec 2021 08:47:48: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 08:47:53: 9000000 INFO @ Fri, 10 Dec 2021 08:48:01: 7000000 INFO @ Fri, 10 Dec 2021 08:48:07: 10000000 INFO @ Fri, 10 Dec 2021 08:48:13: 8000000 INFO @ Fri, 10 Dec 2021 08:48:19: 11000000 INFO @ Fri, 10 Dec 2021 08:48:23: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:48:23: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:48:23: #1 total tags in treatment: 11273626 INFO @ Fri, 10 Dec 2021 08:48:23: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:48:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:48:23: #1 tags after filtering in treatment: 11273626 INFO @ Fri, 10 Dec 2021 08:48:23: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:48:23: #1 finished! INFO @ Fri, 10 Dec 2021 08:48:23: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:48:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:48:24: #2 number of paired peaks: 28 WARNING @ Fri, 10 Dec 2021 08:48:24: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:48:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:48:25: 9000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 08:48:37: 10000000 INFO @ Fri, 10 Dec 2021 08:48:48: 11000000 INFO @ Fri, 10 Dec 2021 08:48:51: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:48:51: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:48:51: #1 total tags in treatment: 11273626 INFO @ Fri, 10 Dec 2021 08:48:51: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:48:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:48:51: #1 tags after filtering in treatment: 11273626 INFO @ Fri, 10 Dec 2021 08:48:51: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:48:51: #1 finished! INFO @ Fri, 10 Dec 2021 08:48:51: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:48:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:48:52: #2 number of paired peaks: 28 WARNING @ Fri, 10 Dec 2021 08:48:52: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:48:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling