Job ID = 10166235 SRX = SRX7723719 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 25811605 spots for SRR11084675/SRR11084675.sra Written 25811605 spots for SRR11084675/SRR11084675.sra fastq に変換しました。 bowtie でマッピング中... Your job 10166614 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:13 25811605 reads; of these: 25811605 (100.00%) were unpaired; of these: 9262418 (35.88%) aligned 0 times 12209555 (47.30%) aligned exactly 1 time 4339632 (16.81%) aligned >1 times 64.12% overall alignment rate Time searching: 00:08:13 Overall time: 00:08:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5275561 / 16549187 = 0.3188 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:55:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:55:07: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:55:07: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:55:13: 1000000 INFO @ Thu, 08 Oct 2020 20:55:19: 2000000 INFO @ Thu, 08 Oct 2020 20:55:25: 3000000 INFO @ Thu, 08 Oct 2020 20:55:31: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:55:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:55:36: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:55:36: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:55:37: 5000000 INFO @ Thu, 08 Oct 2020 20:55:41: 1000000 INFO @ Thu, 08 Oct 2020 20:55:44: 6000000 INFO @ Thu, 08 Oct 2020 20:55:47: 2000000 INFO @ Thu, 08 Oct 2020 20:55:50: 7000000 INFO @ Thu, 08 Oct 2020 20:55:52: 3000000 INFO @ Thu, 08 Oct 2020 20:55:56: 8000000 INFO @ Thu, 08 Oct 2020 20:55:58: 4000000 INFO @ Thu, 08 Oct 2020 20:56:02: 9000000 INFO @ Thu, 08 Oct 2020 20:56:03: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:56:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:56:06: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:56:06: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:56:08: 10000000 INFO @ Thu, 08 Oct 2020 20:56:09: 6000000 INFO @ Thu, 08 Oct 2020 20:56:11: 1000000 INFO @ Thu, 08 Oct 2020 20:56:14: 7000000 INFO @ Thu, 08 Oct 2020 20:56:15: 11000000 INFO @ Thu, 08 Oct 2020 20:56:16: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 20:56:16: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 20:56:16: #1 total tags in treatment: 11273626 INFO @ Thu, 08 Oct 2020 20:56:16: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:56:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:56:16: #1 tags after filtering in treatment: 11273626 INFO @ Thu, 08 Oct 2020 20:56:16: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:56:16: #1 finished! INFO @ Thu, 08 Oct 2020 20:56:16: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:56:16: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:56:17: 2000000 INFO @ Thu, 08 Oct 2020 20:56:17: #2 number of paired peaks: 28 WARNING @ Thu, 08 Oct 2020 20:56:17: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:56:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:56:20: 8000000 INFO @ Thu, 08 Oct 2020 20:56:22: 3000000 INFO @ Thu, 08 Oct 2020 20:56:25: 9000000 INFO @ Thu, 08 Oct 2020 20:56:28: 4000000 INFO @ Thu, 08 Oct 2020 20:56:30: 10000000 INFO @ Thu, 08 Oct 2020 20:56:33: 5000000 INFO @ Thu, 08 Oct 2020 20:56:36: 11000000 INFO @ Thu, 08 Oct 2020 20:56:37: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 20:56:37: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 20:56:37: #1 total tags in treatment: 11273626 INFO @ Thu, 08 Oct 2020 20:56:37: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:56:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:56:37: #1 tags after filtering in treatment: 11273626 INFO @ Thu, 08 Oct 2020 20:56:37: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:56:37: #1 finished! INFO @ Thu, 08 Oct 2020 20:56:37: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:56:37: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:56:38: #2 number of paired peaks: 28 WARNING @ Thu, 08 Oct 2020 20:56:38: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:56:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:56:39: 6000000 INFO @ Thu, 08 Oct 2020 20:56:44: 7000000 INFO @ Thu, 08 Oct 2020 20:56:49: 8000000 INFO @ Thu, 08 Oct 2020 20:56:54: 9000000 INFO @ Thu, 08 Oct 2020 20:56:59: 10000000 INFO @ Thu, 08 Oct 2020 20:57:05: 11000000 INFO @ Thu, 08 Oct 2020 20:57:06: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 20:57:06: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 20:57:06: #1 total tags in treatment: 11273626 INFO @ Thu, 08 Oct 2020 20:57:06: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:57:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:57:06: #1 tags after filtering in treatment: 11273626 INFO @ Thu, 08 Oct 2020 20:57:06: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:57:06: #1 finished! INFO @ Thu, 08 Oct 2020 20:57:06: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:57:06: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:57:07: #2 number of paired peaks: 28 WARNING @ Thu, 08 Oct 2020 20:57:07: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:57:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723719/SRX7723719.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。