Job ID = 10166231 SRX = SRX7723717 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 22112553 spots for SRR11084673/SRR11084673.sra Written 22112553 spots for SRR11084673/SRR11084673.sra fastq に変換しました。 bowtie でマッピング中... Your job 10166609 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:13 22112553 reads; of these: 22112553 (100.00%) were unpaired; of these: 7005762 (31.68%) aligned 0 times 10827683 (48.97%) aligned exactly 1 time 4279108 (19.35%) aligned >1 times 68.32% overall alignment rate Time searching: 00:08:13 Overall time: 00:08:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4691700 / 15106791 = 0.3106 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:54:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:54:21: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:54:21: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:54:27: 1000000 INFO @ Thu, 08 Oct 2020 20:54:33: 2000000 INFO @ Thu, 08 Oct 2020 20:54:39: 3000000 INFO @ Thu, 08 Oct 2020 20:54:44: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:54:50: 5000000 INFO @ Thu, 08 Oct 2020 20:54:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:54:51: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:54:51: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:54:56: 6000000 INFO @ Thu, 08 Oct 2020 20:54:58: 1000000 INFO @ Thu, 08 Oct 2020 20:55:02: 7000000 INFO @ Thu, 08 Oct 2020 20:55:04: 2000000 INFO @ Thu, 08 Oct 2020 20:55:09: 8000000 INFO @ Thu, 08 Oct 2020 20:55:10: 3000000 INFO @ Thu, 08 Oct 2020 20:55:15: 9000000 INFO @ Thu, 08 Oct 2020 20:55:17: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:55:21: 10000000 INFO @ Thu, 08 Oct 2020 20:55:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:55:21: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:55:21: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:55:23: 5000000 INFO @ Thu, 08 Oct 2020 20:55:24: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 20:55:24: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 20:55:24: #1 total tags in treatment: 10415091 INFO @ Thu, 08 Oct 2020 20:55:24: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:55:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:55:24: #1 tags after filtering in treatment: 10415091 INFO @ Thu, 08 Oct 2020 20:55:24: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:55:24: #1 finished! INFO @ Thu, 08 Oct 2020 20:55:24: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:55:24: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:55:25: #2 number of paired peaks: 65 WARNING @ Thu, 08 Oct 2020 20:55:25: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:55:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:55:28: 1000000 INFO @ Thu, 08 Oct 2020 20:55:29: 6000000 INFO @ Thu, 08 Oct 2020 20:55:34: 2000000 INFO @ Thu, 08 Oct 2020 20:55:36: 7000000 INFO @ Thu, 08 Oct 2020 20:55:41: 3000000 INFO @ Thu, 08 Oct 2020 20:55:42: 8000000 INFO @ Thu, 08 Oct 2020 20:55:47: 4000000 INFO @ Thu, 08 Oct 2020 20:55:49: 9000000 INFO @ Thu, 08 Oct 2020 20:55:53: 5000000 INFO @ Thu, 08 Oct 2020 20:55:55: 10000000 INFO @ Thu, 08 Oct 2020 20:55:57: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 20:55:57: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 20:55:57: #1 total tags in treatment: 10415091 INFO @ Thu, 08 Oct 2020 20:55:57: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:55:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:55:58: #1 tags after filtering in treatment: 10415091 INFO @ Thu, 08 Oct 2020 20:55:58: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:55:58: #1 finished! INFO @ Thu, 08 Oct 2020 20:55:58: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:55:58: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:55:58: #2 number of paired peaks: 65 WARNING @ Thu, 08 Oct 2020 20:55:58: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:55:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:55:59: 6000000 INFO @ Thu, 08 Oct 2020 20:56:05: 7000000 INFO @ Thu, 08 Oct 2020 20:56:11: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:56:17: 9000000 INFO @ Thu, 08 Oct 2020 20:56:23: 10000000 INFO @ Thu, 08 Oct 2020 20:56:25: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 20:56:25: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 20:56:25: #1 total tags in treatment: 10415091 INFO @ Thu, 08 Oct 2020 20:56:25: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:56:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:56:25: #1 tags after filtering in treatment: 10415091 INFO @ Thu, 08 Oct 2020 20:56:25: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:56:25: #1 finished! INFO @ Thu, 08 Oct 2020 20:56:25: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:56:25: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:56:26: #2 number of paired peaks: 65 WARNING @ Thu, 08 Oct 2020 20:56:26: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:56:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。