Job ID = 14166984 SRX = SRX7723717 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 22112553 spots for SRR11084673/SRR11084673.sra Written 22112553 spots for SRR11084673/SRR11084673.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167336 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:37 22112553 reads; of these: 22112553 (100.00%) were unpaired; of these: 7005762 (31.68%) aligned 0 times 10827683 (48.97%) aligned exactly 1 time 4279108 (19.35%) aligned >1 times 68.32% overall alignment rate Time searching: 00:11:37 Overall time: 00:11:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4691700 / 15106791 = 0.3106 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:43:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:43:30: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:43:30: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:43:46: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:43:58: 2000000 INFO @ Fri, 10 Dec 2021 08:43:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:43:59: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:43:59: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:44:12: 3000000 INFO @ Fri, 10 Dec 2021 08:44:17: 1000000 INFO @ Fri, 10 Dec 2021 08:44:24: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:44:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:44:30: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:44:30: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:44:31: 2000000 INFO @ Fri, 10 Dec 2021 08:44:38: 5000000 INFO @ Fri, 10 Dec 2021 08:44:42: 3000000 INFO @ Fri, 10 Dec 2021 08:44:50: 6000000 INFO @ Fri, 10 Dec 2021 08:44:51: 1000000 INFO @ Fri, 10 Dec 2021 08:44:51: 4000000 INFO @ Fri, 10 Dec 2021 08:45:01: 5000000 INFO @ Fri, 10 Dec 2021 08:45:02: 7000000 INFO @ Fri, 10 Dec 2021 08:45:11: 6000000 INFO @ Fri, 10 Dec 2021 08:45:13: 2000000 INFO @ Fri, 10 Dec 2021 08:45:14: 8000000 INFO @ Fri, 10 Dec 2021 08:45:24: 7000000 INFO @ Fri, 10 Dec 2021 08:45:27: 9000000 INFO @ Fri, 10 Dec 2021 08:45:34: 8000000 INFO @ Fri, 10 Dec 2021 08:45:37: 3000000 INFO @ Fri, 10 Dec 2021 08:45:38: 10000000 INFO @ Fri, 10 Dec 2021 08:45:42: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:45:42: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:45:42: #1 total tags in treatment: 10415091 INFO @ Fri, 10 Dec 2021 08:45:42: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:45:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:45:42: #1 tags after filtering in treatment: 10415091 INFO @ Fri, 10 Dec 2021 08:45:42: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:45:42: #1 finished! INFO @ Fri, 10 Dec 2021 08:45:42: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:45:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:45:43: #2 number of paired peaks: 65 WARNING @ Fri, 10 Dec 2021 08:45:43: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:45:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:45:46: 9000000 INFO @ Fri, 10 Dec 2021 08:45:58: 10000000 INFO @ Fri, 10 Dec 2021 08:45:59: 4000000 INFO @ Fri, 10 Dec 2021 08:46:03: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:46:03: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:46:03: #1 total tags in treatment: 10415091 INFO @ Fri, 10 Dec 2021 08:46:03: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:46:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:46:03: #1 tags after filtering in treatment: 10415091 INFO @ Fri, 10 Dec 2021 08:46:03: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:46:03: #1 finished! INFO @ Fri, 10 Dec 2021 08:46:03: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:46:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:46:04: #2 number of paired peaks: 65 WARNING @ Fri, 10 Dec 2021 08:46:04: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:46:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 08:46:18: 5000000 INFO @ Fri, 10 Dec 2021 08:46:39: 6000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 08:47:01: 7000000 INFO @ Fri, 10 Dec 2021 08:47:22: 8000000 INFO @ Fri, 10 Dec 2021 08:47:43: 9000000 INFO @ Fri, 10 Dec 2021 08:48:09: 10000000 INFO @ Fri, 10 Dec 2021 08:48:19: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:48:19: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:48:19: #1 total tags in treatment: 10415091 INFO @ Fri, 10 Dec 2021 08:48:19: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:48:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:48:19: #1 tags after filtering in treatment: 10415091 INFO @ Fri, 10 Dec 2021 08:48:19: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:48:19: #1 finished! INFO @ Fri, 10 Dec 2021 08:48:19: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:48:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:48:20: #2 number of paired peaks: 65 WARNING @ Fri, 10 Dec 2021 08:48:20: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:48:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723717/SRX7723717.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling