Job ID = 14166982 SRX = SRX7723715 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 23112749 spots for SRR11084671/SRR11084671.sra Written 23112749 spots for SRR11084671/SRR11084671.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167326 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:03 23112749 reads; of these: 23112749 (100.00%) were unpaired; of these: 2330059 (10.08%) aligned 0 times 15445822 (66.83%) aligned exactly 1 time 5336868 (23.09%) aligned >1 times 89.92% overall alignment rate Time searching: 00:10:04 Overall time: 00:10:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1387613 / 20782690 = 0.0668 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:40:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:40:02: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:40:02: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:40:09: 1000000 INFO @ Fri, 10 Dec 2021 08:40:17: 2000000 INFO @ Fri, 10 Dec 2021 08:40:24: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:40:31: 4000000 INFO @ Fri, 10 Dec 2021 08:40:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:40:32: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:40:32: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:40:39: 5000000 INFO @ Fri, 10 Dec 2021 08:40:39: 1000000 INFO @ Fri, 10 Dec 2021 08:40:47: 6000000 INFO @ Fri, 10 Dec 2021 08:40:47: 2000000 INFO @ Fri, 10 Dec 2021 08:40:55: 7000000 INFO @ Fri, 10 Dec 2021 08:40:55: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:41:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:41:01: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:41:01: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:41:03: 8000000 INFO @ Fri, 10 Dec 2021 08:41:03: 4000000 INFO @ Fri, 10 Dec 2021 08:41:09: 1000000 INFO @ Fri, 10 Dec 2021 08:41:10: 5000000 INFO @ Fri, 10 Dec 2021 08:41:10: 9000000 INFO @ Fri, 10 Dec 2021 08:41:17: 2000000 INFO @ Fri, 10 Dec 2021 08:41:18: 6000000 INFO @ Fri, 10 Dec 2021 08:41:18: 10000000 INFO @ Fri, 10 Dec 2021 08:41:25: 3000000 INFO @ Fri, 10 Dec 2021 08:41:26: 7000000 INFO @ Fri, 10 Dec 2021 08:41:26: 11000000 INFO @ Fri, 10 Dec 2021 08:41:33: 4000000 INFO @ Fri, 10 Dec 2021 08:41:33: 8000000 INFO @ Fri, 10 Dec 2021 08:41:34: 12000000 INFO @ Fri, 10 Dec 2021 08:41:41: 5000000 INFO @ Fri, 10 Dec 2021 08:41:41: 9000000 INFO @ Fri, 10 Dec 2021 08:41:42: 13000000 INFO @ Fri, 10 Dec 2021 08:41:48: 10000000 INFO @ Fri, 10 Dec 2021 08:41:49: 6000000 INFO @ Fri, 10 Dec 2021 08:41:50: 14000000 INFO @ Fri, 10 Dec 2021 08:41:56: 11000000 INFO @ Fri, 10 Dec 2021 08:41:56: 7000000 INFO @ Fri, 10 Dec 2021 08:41:58: 15000000 INFO @ Fri, 10 Dec 2021 08:42:03: 12000000 INFO @ Fri, 10 Dec 2021 08:42:04: 8000000 INFO @ Fri, 10 Dec 2021 08:42:06: 16000000 INFO @ Fri, 10 Dec 2021 08:42:11: 13000000 INFO @ Fri, 10 Dec 2021 08:42:12: 9000000 INFO @ Fri, 10 Dec 2021 08:42:14: 17000000 INFO @ Fri, 10 Dec 2021 08:42:19: 14000000 INFO @ Fri, 10 Dec 2021 08:42:20: 10000000 INFO @ Fri, 10 Dec 2021 08:42:22: 18000000 INFO @ Fri, 10 Dec 2021 08:42:26: 15000000 INFO @ Fri, 10 Dec 2021 08:42:28: 11000000 INFO @ Fri, 10 Dec 2021 08:42:30: 19000000 INFO @ Fri, 10 Dec 2021 08:42:33: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:42:33: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:42:33: #1 total tags in treatment: 19395077 INFO @ Fri, 10 Dec 2021 08:42:33: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:42:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:42:34: #1 tags after filtering in treatment: 19395077 INFO @ Fri, 10 Dec 2021 08:42:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:42:34: #1 finished! INFO @ Fri, 10 Dec 2021 08:42:34: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:42:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:42:34: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 08:42:35: #2 number of paired peaks: 6 WARNING @ Fri, 10 Dec 2021 08:42:35: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:42:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:42:36: 12000000 INFO @ Fri, 10 Dec 2021 08:42:41: 17000000 INFO @ Fri, 10 Dec 2021 08:42:44: 13000000 INFO @ Fri, 10 Dec 2021 08:42:49: 18000000 INFO @ Fri, 10 Dec 2021 08:42:51: 14000000 INFO @ Fri, 10 Dec 2021 08:42:56: 19000000 INFO @ Fri, 10 Dec 2021 08:42:59: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:42:59: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:42:59: #1 total tags in treatment: 19395077 INFO @ Fri, 10 Dec 2021 08:42:59: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:42:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:42:59: 15000000 INFO @ Fri, 10 Dec 2021 08:42:59: #1 tags after filtering in treatment: 19395077 INFO @ Fri, 10 Dec 2021 08:42:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:42:59: #1 finished! INFO @ Fri, 10 Dec 2021 08:42:59: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:42:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:43:01: #2 number of paired peaks: 6 WARNING @ Fri, 10 Dec 2021 08:43:01: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:43:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:43:07: 16000000 INFO @ Fri, 10 Dec 2021 08:43:14: 17000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 08:43:21: 18000000 INFO @ Fri, 10 Dec 2021 08:43:29: 19000000 INFO @ Fri, 10 Dec 2021 08:43:32: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:43:32: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:43:32: #1 total tags in treatment: 19395077 INFO @ Fri, 10 Dec 2021 08:43:32: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:43:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:43:32: #1 tags after filtering in treatment: 19395077 INFO @ Fri, 10 Dec 2021 08:43:32: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:43:32: #1 finished! INFO @ Fri, 10 Dec 2021 08:43:32: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:43:32: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:43:33: #2 number of paired peaks: 6 WARNING @ Fri, 10 Dec 2021 08:43:33: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:43:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723715/SRX7723715.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling