Job ID = 14166971 SRX = SRX7723709 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 62886080 spots for SRR11084665/SRR11084665.sra Written 62886080 spots for SRR11084665/SRR11084665.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167345 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:38 62886080 reads; of these: 62886080 (100.00%) were unpaired; of these: 16456290 (26.17%) aligned 0 times 40499927 (64.40%) aligned exactly 1 time 5929863 (9.43%) aligned >1 times 73.83% overall alignment rate Time searching: 00:22:38 Overall time: 00:22:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 9856250 / 46429790 = 0.2123 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:53:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:53:07: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:53:07: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:53:14: 1000000 INFO @ Fri, 10 Dec 2021 08:53:20: 2000000 INFO @ Fri, 10 Dec 2021 08:53:27: 3000000 INFO @ Fri, 10 Dec 2021 08:53:33: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:53:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:53:37: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:53:37: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:53:40: 5000000 INFO @ Fri, 10 Dec 2021 08:53:44: 1000000 INFO @ Fri, 10 Dec 2021 08:53:47: 6000000 INFO @ Fri, 10 Dec 2021 08:53:51: 2000000 INFO @ Fri, 10 Dec 2021 08:53:54: 7000000 INFO @ Fri, 10 Dec 2021 08:53:57: 3000000 INFO @ Fri, 10 Dec 2021 08:54:01: 8000000 INFO @ Fri, 10 Dec 2021 08:54:04: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:54:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:54:07: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:54:07: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:54:08: 9000000 INFO @ Fri, 10 Dec 2021 08:54:11: 5000000 INFO @ Fri, 10 Dec 2021 08:54:13: 1000000 INFO @ Fri, 10 Dec 2021 08:54:15: 10000000 INFO @ Fri, 10 Dec 2021 08:54:18: 6000000 INFO @ Fri, 10 Dec 2021 08:54:19: 2000000 INFO @ Fri, 10 Dec 2021 08:54:22: 11000000 INFO @ Fri, 10 Dec 2021 08:54:24: 7000000 INFO @ Fri, 10 Dec 2021 08:54:25: 3000000 INFO @ Fri, 10 Dec 2021 08:54:29: 12000000 INFO @ Fri, 10 Dec 2021 08:54:31: 4000000 INFO @ Fri, 10 Dec 2021 08:54:31: 8000000 INFO @ Fri, 10 Dec 2021 08:54:36: 13000000 INFO @ Fri, 10 Dec 2021 08:54:37: 5000000 INFO @ Fri, 10 Dec 2021 08:54:38: 9000000 INFO @ Fri, 10 Dec 2021 08:54:43: 14000000 INFO @ Fri, 10 Dec 2021 08:54:43: 6000000 INFO @ Fri, 10 Dec 2021 08:54:45: 10000000 INFO @ Fri, 10 Dec 2021 08:54:49: 7000000 INFO @ Fri, 10 Dec 2021 08:54:50: 15000000 INFO @ Fri, 10 Dec 2021 08:54:51: 11000000 INFO @ Fri, 10 Dec 2021 08:54:55: 8000000 INFO @ Fri, 10 Dec 2021 08:54:56: 16000000 INFO @ Fri, 10 Dec 2021 08:54:58: 12000000 INFO @ Fri, 10 Dec 2021 08:55:01: 9000000 INFO @ Fri, 10 Dec 2021 08:55:03: 17000000 INFO @ Fri, 10 Dec 2021 08:55:05: 13000000 INFO @ Fri, 10 Dec 2021 08:55:07: 10000000 INFO @ Fri, 10 Dec 2021 08:55:10: 18000000 INFO @ Fri, 10 Dec 2021 08:55:12: 14000000 INFO @ Fri, 10 Dec 2021 08:55:12: 11000000 INFO @ Fri, 10 Dec 2021 08:55:17: 19000000 INFO @ Fri, 10 Dec 2021 08:55:18: 12000000 INFO @ Fri, 10 Dec 2021 08:55:19: 15000000 INFO @ Fri, 10 Dec 2021 08:55:24: 20000000 INFO @ Fri, 10 Dec 2021 08:55:24: 13000000 INFO @ Fri, 10 Dec 2021 08:55:25: 16000000 INFO @ Fri, 10 Dec 2021 08:55:30: 14000000 INFO @ Fri, 10 Dec 2021 08:55:31: 21000000 INFO @ Fri, 10 Dec 2021 08:55:32: 17000000 INFO @ Fri, 10 Dec 2021 08:55:36: 15000000 INFO @ Fri, 10 Dec 2021 08:55:38: 22000000 INFO @ Fri, 10 Dec 2021 08:55:39: 18000000 INFO @ Fri, 10 Dec 2021 08:55:42: 16000000 INFO @ Fri, 10 Dec 2021 08:55:45: 23000000 INFO @ Fri, 10 Dec 2021 08:55:46: 19000000 INFO @ Fri, 10 Dec 2021 08:55:48: 17000000 INFO @ Fri, 10 Dec 2021 08:55:52: 24000000 INFO @ Fri, 10 Dec 2021 08:55:52: 20000000 INFO @ Fri, 10 Dec 2021 08:55:54: 18000000 INFO @ Fri, 10 Dec 2021 08:55:59: 25000000 INFO @ Fri, 10 Dec 2021 08:55:59: 21000000 INFO @ Fri, 10 Dec 2021 08:56:00: 19000000 INFO @ Fri, 10 Dec 2021 08:56:06: 22000000 INFO @ Fri, 10 Dec 2021 08:56:06: 20000000 INFO @ Fri, 10 Dec 2021 08:56:06: 26000000 INFO @ Fri, 10 Dec 2021 08:56:12: 21000000 INFO @ Fri, 10 Dec 2021 08:56:13: 23000000 INFO @ Fri, 10 Dec 2021 08:56:13: 27000000 INFO @ Fri, 10 Dec 2021 08:56:18: 22000000 INFO @ Fri, 10 Dec 2021 08:56:19: 24000000 INFO @ Fri, 10 Dec 2021 08:56:20: 28000000 INFO @ Fri, 10 Dec 2021 08:56:24: 23000000 INFO @ Fri, 10 Dec 2021 08:56:26: 25000000 INFO @ Fri, 10 Dec 2021 08:56:27: 29000000 INFO @ Fri, 10 Dec 2021 08:56:30: 24000000 INFO @ Fri, 10 Dec 2021 08:56:33: 26000000 INFO @ Fri, 10 Dec 2021 08:56:34: 30000000 INFO @ Fri, 10 Dec 2021 08:56:36: 25000000 INFO @ Fri, 10 Dec 2021 08:56:40: 27000000 INFO @ Fri, 10 Dec 2021 08:56:41: 31000000 INFO @ Fri, 10 Dec 2021 08:56:42: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 08:56:46: 28000000 INFO @ Fri, 10 Dec 2021 08:56:48: 27000000 INFO @ Fri, 10 Dec 2021 08:56:48: 32000000 INFO @ Fri, 10 Dec 2021 08:56:53: 29000000 INFO @ Fri, 10 Dec 2021 08:56:54: 28000000 INFO @ Fri, 10 Dec 2021 08:56:55: 33000000 INFO @ Fri, 10 Dec 2021 08:57:00: 29000000 INFO @ Fri, 10 Dec 2021 08:57:00: 30000000 INFO @ Fri, 10 Dec 2021 08:57:02: 34000000 INFO @ Fri, 10 Dec 2021 08:57:06: 30000000 INFO @ Fri, 10 Dec 2021 08:57:07: 31000000 INFO @ Fri, 10 Dec 2021 08:57:09: 35000000 INFO @ Fri, 10 Dec 2021 08:57:12: 31000000 INFO @ Fri, 10 Dec 2021 08:57:14: 32000000 INFO @ Fri, 10 Dec 2021 08:57:16: 36000000 INFO @ Fri, 10 Dec 2021 08:57:18: 32000000 INFO @ Fri, 10 Dec 2021 08:57:20: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:57:20: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:57:20: #1 total tags in treatment: 36573540 INFO @ Fri, 10 Dec 2021 08:57:20: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:57:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:57:20: 33000000 INFO @ Fri, 10 Dec 2021 08:57:21: #1 tags after filtering in treatment: 36573540 INFO @ Fri, 10 Dec 2021 08:57:21: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:57:21: #1 finished! INFO @ Fri, 10 Dec 2021 08:57:21: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:57:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:57:23: #2 number of paired peaks: 46 WARNING @ Fri, 10 Dec 2021 08:57:23: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:57:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:57:24: 33000000 INFO @ Fri, 10 Dec 2021 08:57:27: 34000000 INFO @ Fri, 10 Dec 2021 08:57:29: 34000000 INFO @ Fri, 10 Dec 2021 08:57:34: 35000000 INFO @ Fri, 10 Dec 2021 08:57:35: 35000000 INFO @ Fri, 10 Dec 2021 08:57:41: 36000000 INFO @ Fri, 10 Dec 2021 08:57:41: 36000000 INFO @ Fri, 10 Dec 2021 08:57:45: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:57:45: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:57:45: #1 total tags in treatment: 36573540 INFO @ Fri, 10 Dec 2021 08:57:45: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:57:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:57:45: #1 tags after filtering in treatment: 36573540 INFO @ Fri, 10 Dec 2021 08:57:45: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:57:45: #1 finished! INFO @ Fri, 10 Dec 2021 08:57:45: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:57:45: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:57:46: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:57:46: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:57:46: #1 total tags in treatment: 36573540 INFO @ Fri, 10 Dec 2021 08:57:46: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:57:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:57:46: #1 tags after filtering in treatment: 36573540 INFO @ Fri, 10 Dec 2021 08:57:46: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:57:46: #1 finished! INFO @ Fri, 10 Dec 2021 08:57:46: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:57:46: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:57:48: #2 number of paired peaks: 46 WARNING @ Fri, 10 Dec 2021 08:57:48: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:57:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:57:48: #2 number of paired peaks: 46 WARNING @ Fri, 10 Dec 2021 08:57:48: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:57:48: Process for pairing-model is terminated! BigWig に変換しました。 cut: /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling