Job ID = 10166187 SRX = SRX7723709 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 62886080 spots for SRR11084665/SRR11084665.sra Written 62886080 spots for SRR11084665/SRR11084665.sra fastq に変換しました。 bowtie でマッピング中... Your job 10166693 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:29 62886080 reads; of these: 62886080 (100.00%) were unpaired; of these: 16456290 (26.17%) aligned 0 times 40499927 (64.40%) aligned exactly 1 time 5929863 (9.43%) aligned >1 times 73.83% overall alignment rate Time searching: 00:22:29 Overall time: 00:22:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 9856250 / 46429790 = 0.2123 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:14:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:14:34: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:14:34: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:14:40: 1000000 INFO @ Thu, 08 Oct 2020 21:14:45: 2000000 INFO @ Thu, 08 Oct 2020 21:14:50: 3000000 INFO @ Thu, 08 Oct 2020 21:14:56: 4000000 INFO @ Thu, 08 Oct 2020 21:15:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:15:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:15:04: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:15:04: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:15:07: 6000000 INFO @ Thu, 08 Oct 2020 21:15:10: 1000000 INFO @ Thu, 08 Oct 2020 21:15:13: 7000000 INFO @ Thu, 08 Oct 2020 21:15:17: 2000000 INFO @ Thu, 08 Oct 2020 21:15:18: 8000000 INFO @ Thu, 08 Oct 2020 21:15:23: 3000000 INFO @ Thu, 08 Oct 2020 21:15:24: 9000000 INFO @ Thu, 08 Oct 2020 21:15:30: 4000000 INFO @ Thu, 08 Oct 2020 21:15:30: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:15:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:15:34: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:15:34: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:15:36: 11000000 INFO @ Thu, 08 Oct 2020 21:15:36: 5000000 INFO @ Thu, 08 Oct 2020 21:15:41: 1000000 INFO @ Thu, 08 Oct 2020 21:15:42: 12000000 INFO @ Thu, 08 Oct 2020 21:15:43: 6000000 INFO @ Thu, 08 Oct 2020 21:15:47: 2000000 INFO @ Thu, 08 Oct 2020 21:15:48: 13000000 INFO @ Thu, 08 Oct 2020 21:15:50: 7000000 INFO @ Thu, 08 Oct 2020 21:15:54: 14000000 INFO @ Thu, 08 Oct 2020 21:15:54: 3000000 INFO @ Thu, 08 Oct 2020 21:15:56: 8000000 INFO @ Thu, 08 Oct 2020 21:16:00: 15000000 INFO @ Thu, 08 Oct 2020 21:16:01: 4000000 INFO @ Thu, 08 Oct 2020 21:16:03: 9000000 INFO @ Thu, 08 Oct 2020 21:16:06: 16000000 INFO @ Thu, 08 Oct 2020 21:16:07: 5000000 INFO @ Thu, 08 Oct 2020 21:16:09: 10000000 INFO @ Thu, 08 Oct 2020 21:16:12: 17000000 INFO @ Thu, 08 Oct 2020 21:16:14: 6000000 INFO @ Thu, 08 Oct 2020 21:16:16: 11000000 INFO @ Thu, 08 Oct 2020 21:16:18: 18000000 INFO @ Thu, 08 Oct 2020 21:16:20: 7000000 INFO @ Thu, 08 Oct 2020 21:16:23: 12000000 INFO @ Thu, 08 Oct 2020 21:16:24: 19000000 INFO @ Thu, 08 Oct 2020 21:16:27: 8000000 INFO @ Thu, 08 Oct 2020 21:16:29: 13000000 INFO @ Thu, 08 Oct 2020 21:16:30: 20000000 INFO @ Thu, 08 Oct 2020 21:16:33: 9000000 INFO @ Thu, 08 Oct 2020 21:16:36: 14000000 INFO @ Thu, 08 Oct 2020 21:16:36: 21000000 INFO @ Thu, 08 Oct 2020 21:16:40: 10000000 INFO @ Thu, 08 Oct 2020 21:16:42: 22000000 INFO @ Thu, 08 Oct 2020 21:16:43: 15000000 INFO @ Thu, 08 Oct 2020 21:16:47: 11000000 INFO @ Thu, 08 Oct 2020 21:16:48: 23000000 INFO @ Thu, 08 Oct 2020 21:16:50: 16000000 INFO @ Thu, 08 Oct 2020 21:16:53: 12000000 INFO @ Thu, 08 Oct 2020 21:16:54: 24000000 INFO @ Thu, 08 Oct 2020 21:16:56: 17000000 INFO @ Thu, 08 Oct 2020 21:17:00: 13000000 INFO @ Thu, 08 Oct 2020 21:17:00: 25000000 INFO @ Thu, 08 Oct 2020 21:17:03: 18000000 INFO @ Thu, 08 Oct 2020 21:17:06: 26000000 INFO @ Thu, 08 Oct 2020 21:17:07: 14000000 INFO @ Thu, 08 Oct 2020 21:17:10: 19000000 INFO @ Thu, 08 Oct 2020 21:17:12: 27000000 INFO @ Thu, 08 Oct 2020 21:17:13: 15000000 INFO @ Thu, 08 Oct 2020 21:17:17: 20000000 INFO @ Thu, 08 Oct 2020 21:17:18: 28000000 INFO @ Thu, 08 Oct 2020 21:17:20: 16000000 INFO @ Thu, 08 Oct 2020 21:17:23: 21000000 INFO @ Thu, 08 Oct 2020 21:17:24: 29000000 INFO @ Thu, 08 Oct 2020 21:17:27: 17000000 INFO @ Thu, 08 Oct 2020 21:17:30: 30000000 INFO @ Thu, 08 Oct 2020 21:17:30: 22000000 INFO @ Thu, 08 Oct 2020 21:17:34: 18000000 INFO @ Thu, 08 Oct 2020 21:17:36: 31000000 INFO @ Thu, 08 Oct 2020 21:17:37: 23000000 INFO @ Thu, 08 Oct 2020 21:17:41: 19000000 INFO @ Thu, 08 Oct 2020 21:17:42: 32000000 INFO @ Thu, 08 Oct 2020 21:17:44: 24000000 INFO @ Thu, 08 Oct 2020 21:17:48: 20000000 INFO @ Thu, 08 Oct 2020 21:17:48: 33000000 INFO @ Thu, 08 Oct 2020 21:17:50: 25000000 INFO @ Thu, 08 Oct 2020 21:17:54: 34000000 INFO @ Thu, 08 Oct 2020 21:17:54: 21000000 INFO @ Thu, 08 Oct 2020 21:17:57: 26000000 INFO @ Thu, 08 Oct 2020 21:18:00: 35000000 INFO @ Thu, 08 Oct 2020 21:18:01: 22000000 INFO @ Thu, 08 Oct 2020 21:18:03: 27000000 INFO @ Thu, 08 Oct 2020 21:18:06: 36000000 INFO @ Thu, 08 Oct 2020 21:18:08: 23000000 INFO @ Thu, 08 Oct 2020 21:18:10: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:18:10: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:18:10: #1 total tags in treatment: 36573540 INFO @ Thu, 08 Oct 2020 21:18:10: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:18:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:18:10: 28000000 INFO @ Thu, 08 Oct 2020 21:18:11: #1 tags after filtering in treatment: 36573540 INFO @ Thu, 08 Oct 2020 21:18:11: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:18:11: #1 finished! INFO @ Thu, 08 Oct 2020 21:18:11: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:18:11: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:18:13: #2 number of paired peaks: 46 WARNING @ Thu, 08 Oct 2020 21:18:13: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:18:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 21:18:15: 24000000 INFO @ Thu, 08 Oct 2020 21:18:17: 29000000 INFO @ Thu, 08 Oct 2020 21:18:22: 25000000 INFO @ Thu, 08 Oct 2020 21:18:24: 30000000 INFO @ Thu, 08 Oct 2020 21:18:29: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 21:18:30: 31000000 INFO @ Thu, 08 Oct 2020 21:18:35: 27000000 INFO @ Thu, 08 Oct 2020 21:18:37: 32000000 INFO @ Thu, 08 Oct 2020 21:18:42: 28000000 INFO @ Thu, 08 Oct 2020 21:18:44: 33000000 INFO @ Thu, 08 Oct 2020 21:18:49: 29000000 INFO @ Thu, 08 Oct 2020 21:18:50: 34000000 INFO @ Thu, 08 Oct 2020 21:18:56: 30000000 INFO @ Thu, 08 Oct 2020 21:18:57: 35000000 INFO @ Thu, 08 Oct 2020 21:19:02: 31000000 INFO @ Thu, 08 Oct 2020 21:19:04: 36000000 INFO @ Thu, 08 Oct 2020 21:19:08: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:19:08: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:19:08: #1 total tags in treatment: 36573540 INFO @ Thu, 08 Oct 2020 21:19:08: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:19:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:19:08: #1 tags after filtering in treatment: 36573540 INFO @ Thu, 08 Oct 2020 21:19:08: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:19:08: #1 finished! INFO @ Thu, 08 Oct 2020 21:19:08: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:19:08: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:19:09: 32000000 INFO @ Thu, 08 Oct 2020 21:19:11: #2 number of paired peaks: 46 WARNING @ Thu, 08 Oct 2020 21:19:11: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:19:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 21:19:15: 33000000 INFO @ Thu, 08 Oct 2020 21:19:21: 34000000 INFO @ Thu, 08 Oct 2020 21:19:28: 35000000 INFO @ Thu, 08 Oct 2020 21:19:34: 36000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 21:19:38: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:19:38: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:19:38: #1 total tags in treatment: 36573540 INFO @ Thu, 08 Oct 2020 21:19:38: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:19:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:19:38: #1 tags after filtering in treatment: 36573540 INFO @ Thu, 08 Oct 2020 21:19:38: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:19:38: #1 finished! INFO @ Thu, 08 Oct 2020 21:19:38: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:19:38: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:19:41: #2 number of paired peaks: 46 WARNING @ Thu, 08 Oct 2020 21:19:41: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:19:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723709/SRX7723709.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling