Job ID = 14166969 SRX = SRX7723707 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 40384595 spots for SRR11084663/SRR11084663.sra Written 40384595 spots for SRR11084663/SRR11084663.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167337 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:35 40384595 reads; of these: 40384595 (100.00%) were unpaired; of these: 10684356 (26.46%) aligned 0 times 22329636 (55.29%) aligned exactly 1 time 7370603 (18.25%) aligned >1 times 73.54% overall alignment rate Time searching: 00:21:35 Overall time: 00:21:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 10282223 / 29700239 = 0.3462 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:45:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:45:56: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:45:56: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:46:02: 1000000 INFO @ Fri, 10 Dec 2021 08:46:08: 2000000 INFO @ Fri, 10 Dec 2021 08:46:13: 3000000 INFO @ Fri, 10 Dec 2021 08:46:19: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:46:25: 5000000 INFO @ Fri, 10 Dec 2021 08:46:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:46:26: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:46:26: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:46:31: 6000000 INFO @ Fri, 10 Dec 2021 08:46:33: 1000000 INFO @ Fri, 10 Dec 2021 08:46:38: 7000000 INFO @ Fri, 10 Dec 2021 08:46:39: 2000000 INFO @ Fri, 10 Dec 2021 08:46:44: 8000000 INFO @ Fri, 10 Dec 2021 08:46:46: 3000000 INFO @ Fri, 10 Dec 2021 08:46:50: 9000000 INFO @ Fri, 10 Dec 2021 08:46:52: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:46:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:46:56: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:46:56: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:46:57: 10000000 INFO @ Fri, 10 Dec 2021 08:46:59: 5000000 INFO @ Fri, 10 Dec 2021 08:47:03: 1000000 INFO @ Fri, 10 Dec 2021 08:47:03: 11000000 INFO @ Fri, 10 Dec 2021 08:47:05: 6000000 INFO @ Fri, 10 Dec 2021 08:47:09: 2000000 INFO @ Fri, 10 Dec 2021 08:47:10: 12000000 INFO @ Fri, 10 Dec 2021 08:47:12: 7000000 INFO @ Fri, 10 Dec 2021 08:47:16: 3000000 INFO @ Fri, 10 Dec 2021 08:47:16: 13000000 INFO @ Fri, 10 Dec 2021 08:47:18: 8000000 INFO @ Fri, 10 Dec 2021 08:47:22: 14000000 INFO @ Fri, 10 Dec 2021 08:47:22: 4000000 INFO @ Fri, 10 Dec 2021 08:47:25: 9000000 INFO @ Fri, 10 Dec 2021 08:47:29: 15000000 INFO @ Fri, 10 Dec 2021 08:47:29: 5000000 INFO @ Fri, 10 Dec 2021 08:47:31: 10000000 INFO @ Fri, 10 Dec 2021 08:47:35: 16000000 INFO @ Fri, 10 Dec 2021 08:47:35: 6000000 INFO @ Fri, 10 Dec 2021 08:47:37: 11000000 INFO @ Fri, 10 Dec 2021 08:47:41: 17000000 INFO @ Fri, 10 Dec 2021 08:47:41: 7000000 INFO @ Fri, 10 Dec 2021 08:47:44: 12000000 INFO @ Fri, 10 Dec 2021 08:47:47: 18000000 INFO @ Fri, 10 Dec 2021 08:47:48: 8000000 INFO @ Fri, 10 Dec 2021 08:47:50: 13000000 INFO @ Fri, 10 Dec 2021 08:47:54: 19000000 INFO @ Fri, 10 Dec 2021 08:47:54: 9000000 INFO @ Fri, 10 Dec 2021 08:47:56: 14000000 INFO @ Fri, 10 Dec 2021 08:47:57: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:47:57: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:47:57: #1 total tags in treatment: 19418016 INFO @ Fri, 10 Dec 2021 08:47:57: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:47:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:47:57: #1 tags after filtering in treatment: 19418016 INFO @ Fri, 10 Dec 2021 08:47:57: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:47:57: #1 finished! INFO @ Fri, 10 Dec 2021 08:47:57: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:47:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:47:58: #2 number of paired peaks: 21 WARNING @ Fri, 10 Dec 2021 08:47:58: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:47:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:48:00: 10000000 INFO @ Fri, 10 Dec 2021 08:48:03: 15000000 INFO @ Fri, 10 Dec 2021 08:48:07: 11000000 INFO @ Fri, 10 Dec 2021 08:48:09: 16000000 INFO @ Fri, 10 Dec 2021 08:48:13: 12000000 INFO @ Fri, 10 Dec 2021 08:48:15: 17000000 INFO @ Fri, 10 Dec 2021 08:48:19: 13000000 INFO @ Fri, 10 Dec 2021 08:48:22: 18000000 INFO @ Fri, 10 Dec 2021 08:48:26: 14000000 INFO @ Fri, 10 Dec 2021 08:48:28: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 08:48:31: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:48:31: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:48:31: #1 total tags in treatment: 19418016 INFO @ Fri, 10 Dec 2021 08:48:31: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:48:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:48:31: #1 tags after filtering in treatment: 19418016 INFO @ Fri, 10 Dec 2021 08:48:31: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:48:31: #1 finished! INFO @ Fri, 10 Dec 2021 08:48:31: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:48:31: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:48:32: 15000000 INFO @ Fri, 10 Dec 2021 08:48:32: #2 number of paired peaks: 21 WARNING @ Fri, 10 Dec 2021 08:48:32: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:48:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:48:37: 16000000 INFO @ Fri, 10 Dec 2021 08:48:43: 17000000 INFO @ Fri, 10 Dec 2021 08:48:49: 18000000 INFO @ Fri, 10 Dec 2021 08:48:54: 19000000 INFO @ Fri, 10 Dec 2021 08:48:57: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:48:57: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:48:57: #1 total tags in treatment: 19418016 INFO @ Fri, 10 Dec 2021 08:48:57: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:48:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:48:57: #1 tags after filtering in treatment: 19418016 INFO @ Fri, 10 Dec 2021 08:48:57: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:48:57: #1 finished! INFO @ Fri, 10 Dec 2021 08:48:57: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:48:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:48:58: #2 number of paired peaks: 21 WARNING @ Fri, 10 Dec 2021 08:48:58: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:48:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。