Job ID = 10166185 SRX = SRX7723707 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 40384595 spots for SRR11084663/SRR11084663.sra Written 40384595 spots for SRR11084663/SRR11084663.sra fastq に変換しました。 bowtie でマッピング中... Your job 10166638 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:24 40384595 reads; of these: 40384595 (100.00%) were unpaired; of these: 10684356 (26.46%) aligned 0 times 22329636 (55.29%) aligned exactly 1 time 7370603 (18.25%) aligned >1 times 73.54% overall alignment rate Time searching: 00:17:24 Overall time: 00:17:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 10282223 / 29700239 = 0.3462 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:02:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:02:34: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:02:34: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:02:40: 1000000 INFO @ Thu, 08 Oct 2020 21:02:46: 2000000 INFO @ Thu, 08 Oct 2020 21:02:51: 3000000 INFO @ Thu, 08 Oct 2020 21:02:57: 4000000 INFO @ Thu, 08 Oct 2020 21:03:02: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:03:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:03:04: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:03:04: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:03:08: 6000000 INFO @ Thu, 08 Oct 2020 21:03:11: 1000000 INFO @ Thu, 08 Oct 2020 21:03:14: 7000000 INFO @ Thu, 08 Oct 2020 21:03:17: 2000000 INFO @ Thu, 08 Oct 2020 21:03:20: 8000000 INFO @ Thu, 08 Oct 2020 21:03:23: 3000000 INFO @ Thu, 08 Oct 2020 21:03:26: 9000000 INFO @ Thu, 08 Oct 2020 21:03:29: 4000000 INFO @ Thu, 08 Oct 2020 21:03:32: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:03:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:03:34: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:03:34: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:03:35: 5000000 INFO @ Thu, 08 Oct 2020 21:03:39: 11000000 INFO @ Thu, 08 Oct 2020 21:03:41: 6000000 INFO @ Thu, 08 Oct 2020 21:03:42: 1000000 INFO @ Thu, 08 Oct 2020 21:03:45: 12000000 INFO @ Thu, 08 Oct 2020 21:03:48: 7000000 INFO @ Thu, 08 Oct 2020 21:03:49: 2000000 INFO @ Thu, 08 Oct 2020 21:03:51: 13000000 INFO @ Thu, 08 Oct 2020 21:03:54: 8000000 INFO @ Thu, 08 Oct 2020 21:03:56: 3000000 INFO @ Thu, 08 Oct 2020 21:03:58: 14000000 INFO @ Thu, 08 Oct 2020 21:04:01: 9000000 INFO @ Thu, 08 Oct 2020 21:04:03: 4000000 INFO @ Thu, 08 Oct 2020 21:04:04: 15000000 INFO @ Thu, 08 Oct 2020 21:04:07: 10000000 INFO @ Thu, 08 Oct 2020 21:04:10: 5000000 INFO @ Thu, 08 Oct 2020 21:04:11: 16000000 INFO @ Thu, 08 Oct 2020 21:04:13: 11000000 INFO @ Thu, 08 Oct 2020 21:04:17: 6000000 INFO @ Thu, 08 Oct 2020 21:04:17: 17000000 INFO @ Thu, 08 Oct 2020 21:04:20: 12000000 INFO @ Thu, 08 Oct 2020 21:04:24: 18000000 INFO @ Thu, 08 Oct 2020 21:04:24: 7000000 INFO @ Thu, 08 Oct 2020 21:04:26: 13000000 INFO @ Thu, 08 Oct 2020 21:04:30: 19000000 INFO @ Thu, 08 Oct 2020 21:04:31: 8000000 INFO @ Thu, 08 Oct 2020 21:04:33: 14000000 INFO @ Thu, 08 Oct 2020 21:04:33: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:04:33: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:04:33: #1 total tags in treatment: 19418016 INFO @ Thu, 08 Oct 2020 21:04:33: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:04:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:04:33: #1 tags after filtering in treatment: 19418016 INFO @ Thu, 08 Oct 2020 21:04:33: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:04:33: #1 finished! INFO @ Thu, 08 Oct 2020 21:04:33: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:04:33: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:04:34: #2 number of paired peaks: 21 WARNING @ Thu, 08 Oct 2020 21:04:34: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:04:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 21:04:38: 9000000 INFO @ Thu, 08 Oct 2020 21:04:39: 15000000 INFO @ Thu, 08 Oct 2020 21:04:45: 16000000 INFO @ Thu, 08 Oct 2020 21:04:46: 10000000 INFO @ Thu, 08 Oct 2020 21:04:51: 17000000 INFO @ Thu, 08 Oct 2020 21:04:53: 11000000 INFO @ Thu, 08 Oct 2020 21:04:58: 18000000 INFO @ Thu, 08 Oct 2020 21:05:00: 12000000 INFO @ Thu, 08 Oct 2020 21:05:04: 19000000 INFO @ Thu, 08 Oct 2020 21:05:06: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:05:06: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:05:06: #1 total tags in treatment: 19418016 INFO @ Thu, 08 Oct 2020 21:05:06: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:05:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:05:07: 13000000 INFO @ Thu, 08 Oct 2020 21:05:07: #1 tags after filtering in treatment: 19418016 INFO @ Thu, 08 Oct 2020 21:05:07: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:05:07: #1 finished! INFO @ Thu, 08 Oct 2020 21:05:07: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:05:07: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 21:05:08: #2 number of paired peaks: 21 WARNING @ Thu, 08 Oct 2020 21:05:08: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:05:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 21:05:14: 14000000 INFO @ Thu, 08 Oct 2020 21:05:20: 15000000 INFO @ Thu, 08 Oct 2020 21:05:27: 16000000 INFO @ Thu, 08 Oct 2020 21:05:33: 17000000 INFO @ Thu, 08 Oct 2020 21:05:40: 18000000 INFO @ Thu, 08 Oct 2020 21:05:47: 19000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 21:05:49: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:05:49: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:05:49: #1 total tags in treatment: 19418016 INFO @ Thu, 08 Oct 2020 21:05:49: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:05:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:05:50: #1 tags after filtering in treatment: 19418016 INFO @ Thu, 08 Oct 2020 21:05:50: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:05:50: #1 finished! INFO @ Thu, 08 Oct 2020 21:05:50: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:05:50: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:05:51: #2 number of paired peaks: 21 WARNING @ Thu, 08 Oct 2020 21:05:51: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:05:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723707/SRX7723707.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling