Job ID = 14166965 SRX = SRX7723703 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 51994999 spots for SRR11084659/SRR11084659.sra Written 51994999 spots for SRR11084659/SRR11084659.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167314 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:24 51994999 reads; of these: 51994999 (100.00%) were unpaired; of these: 12755887 (24.53%) aligned 0 times 34575940 (66.50%) aligned exactly 1 time 4663172 (8.97%) aligned >1 times 75.47% overall alignment rate Time searching: 00:18:25 Overall time: 00:18:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6269542 / 39239112 = 0.1598 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:41:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:41:52: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:41:52: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:42:01: 1000000 INFO @ Fri, 10 Dec 2021 08:42:10: 2000000 INFO @ Fri, 10 Dec 2021 08:42:18: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:42:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:42:21: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:42:21: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:42:28: 4000000 INFO @ Fri, 10 Dec 2021 08:42:29: 1000000 INFO @ Fri, 10 Dec 2021 08:42:36: 2000000 INFO @ Fri, 10 Dec 2021 08:42:38: 5000000 INFO @ Fri, 10 Dec 2021 08:42:44: 3000000 INFO @ Fri, 10 Dec 2021 08:42:47: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:42:51: 4000000 INFO @ Fri, 10 Dec 2021 08:42:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:42:52: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:42:52: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:42:58: 7000000 INFO @ Fri, 10 Dec 2021 08:42:59: 5000000 INFO @ Fri, 10 Dec 2021 08:43:00: 1000000 INFO @ Fri, 10 Dec 2021 08:43:07: 6000000 INFO @ Fri, 10 Dec 2021 08:43:07: 8000000 INFO @ Fri, 10 Dec 2021 08:43:08: 2000000 INFO @ Fri, 10 Dec 2021 08:43:15: 7000000 INFO @ Fri, 10 Dec 2021 08:43:15: 3000000 INFO @ Fri, 10 Dec 2021 08:43:17: 9000000 INFO @ Fri, 10 Dec 2021 08:43:23: 8000000 INFO @ Fri, 10 Dec 2021 08:43:23: 4000000 INFO @ Fri, 10 Dec 2021 08:43:27: 10000000 INFO @ Fri, 10 Dec 2021 08:43:31: 9000000 INFO @ Fri, 10 Dec 2021 08:43:31: 5000000 INFO @ Fri, 10 Dec 2021 08:43:37: 11000000 INFO @ Fri, 10 Dec 2021 08:43:38: 10000000 INFO @ Fri, 10 Dec 2021 08:43:39: 6000000 INFO @ Fri, 10 Dec 2021 08:43:46: 11000000 INFO @ Fri, 10 Dec 2021 08:43:46: 12000000 INFO @ Fri, 10 Dec 2021 08:43:47: 7000000 INFO @ Fri, 10 Dec 2021 08:43:54: 12000000 INFO @ Fri, 10 Dec 2021 08:43:54: 8000000 INFO @ Fri, 10 Dec 2021 08:43:56: 13000000 INFO @ Fri, 10 Dec 2021 08:44:01: 13000000 INFO @ Fri, 10 Dec 2021 08:44:02: 9000000 INFO @ Fri, 10 Dec 2021 08:44:05: 14000000 INFO @ Fri, 10 Dec 2021 08:44:09: 14000000 INFO @ Fri, 10 Dec 2021 08:44:10: 10000000 INFO @ Fri, 10 Dec 2021 08:44:15: 15000000 INFO @ Fri, 10 Dec 2021 08:44:17: 15000000 INFO @ Fri, 10 Dec 2021 08:44:17: 11000000 INFO @ Fri, 10 Dec 2021 08:44:24: 16000000 INFO @ Fri, 10 Dec 2021 08:44:25: 16000000 INFO @ Fri, 10 Dec 2021 08:44:25: 12000000 INFO @ Fri, 10 Dec 2021 08:44:33: 13000000 INFO @ Fri, 10 Dec 2021 08:44:34: 17000000 INFO @ Fri, 10 Dec 2021 08:44:34: 17000000 INFO @ Fri, 10 Dec 2021 08:44:40: 14000000 INFO @ Fri, 10 Dec 2021 08:44:41: 18000000 INFO @ Fri, 10 Dec 2021 08:44:44: 18000000 INFO @ Fri, 10 Dec 2021 08:44:48: 15000000 INFO @ Fri, 10 Dec 2021 08:44:49: 19000000 INFO @ Fri, 10 Dec 2021 08:44:53: 19000000 INFO @ Fri, 10 Dec 2021 08:44:56: 16000000 INFO @ Fri, 10 Dec 2021 08:44:57: 20000000 INFO @ Fri, 10 Dec 2021 08:45:03: 20000000 INFO @ Fri, 10 Dec 2021 08:45:03: 17000000 INFO @ Fri, 10 Dec 2021 08:45:04: 21000000 INFO @ Fri, 10 Dec 2021 08:45:11: 18000000 INFO @ Fri, 10 Dec 2021 08:45:12: 22000000 INFO @ Fri, 10 Dec 2021 08:45:12: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 08:45:18: 19000000 INFO @ Fri, 10 Dec 2021 08:45:19: 23000000 INFO @ Fri, 10 Dec 2021 08:45:22: 22000000 INFO @ Fri, 10 Dec 2021 08:45:25: 20000000 INFO @ Fri, 10 Dec 2021 08:45:27: 24000000 INFO @ Fri, 10 Dec 2021 08:45:32: 23000000 INFO @ Fri, 10 Dec 2021 08:45:33: 21000000 INFO @ Fri, 10 Dec 2021 08:45:34: 25000000 INFO @ Fri, 10 Dec 2021 08:45:41: 22000000 INFO @ Fri, 10 Dec 2021 08:45:41: 24000000 INFO @ Fri, 10 Dec 2021 08:45:42: 26000000 INFO @ Fri, 10 Dec 2021 08:45:48: 23000000 INFO @ Fri, 10 Dec 2021 08:45:50: 27000000 INFO @ Fri, 10 Dec 2021 08:45:51: 25000000 INFO @ Fri, 10 Dec 2021 08:45:55: 24000000 INFO @ Fri, 10 Dec 2021 08:45:57: 28000000 INFO @ Fri, 10 Dec 2021 08:46:01: 26000000 INFO @ Fri, 10 Dec 2021 08:46:03: 25000000 INFO @ Fri, 10 Dec 2021 08:46:05: 29000000 INFO @ Fri, 10 Dec 2021 08:46:10: 26000000 INFO @ Fri, 10 Dec 2021 08:46:11: 27000000 INFO @ Fri, 10 Dec 2021 08:46:12: 30000000 INFO @ Fri, 10 Dec 2021 08:46:18: 27000000 INFO @ Fri, 10 Dec 2021 08:46:20: 31000000 INFO @ Fri, 10 Dec 2021 08:46:21: 28000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 08:46:26: 28000000 INFO @ Fri, 10 Dec 2021 08:46:28: 32000000 INFO @ Fri, 10 Dec 2021 08:46:31: 29000000 INFO @ Fri, 10 Dec 2021 08:46:34: 29000000 INFO @ Fri, 10 Dec 2021 08:46:36: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:46:36: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:46:36: #1 total tags in treatment: 32969570 INFO @ Fri, 10 Dec 2021 08:46:36: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:46:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:46:36: #1 tags after filtering in treatment: 32969570 INFO @ Fri, 10 Dec 2021 08:46:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:46:36: #1 finished! INFO @ Fri, 10 Dec 2021 08:46:36: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:46:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:46:38: #2 number of paired peaks: 24 WARNING @ Fri, 10 Dec 2021 08:46:38: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:46:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:46:41: 30000000 INFO @ Fri, 10 Dec 2021 08:46:41: 30000000 INFO @ Fri, 10 Dec 2021 08:46:50: 31000000 INFO @ Fri, 10 Dec 2021 08:46:51: 31000000 INFO @ Fri, 10 Dec 2021 08:46:57: 32000000 INFO @ Fri, 10 Dec 2021 08:47:01: 32000000 INFO @ Fri, 10 Dec 2021 08:47:05: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:47:05: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:47:05: #1 total tags in treatment: 32969570 INFO @ Fri, 10 Dec 2021 08:47:05: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:47:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:47:05: #1 tags after filtering in treatment: 32969570 INFO @ Fri, 10 Dec 2021 08:47:05: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:47:05: #1 finished! INFO @ Fri, 10 Dec 2021 08:47:05: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:47:05: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:47:07: #2 number of paired peaks: 24 WARNING @ Fri, 10 Dec 2021 08:47:07: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:47:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:47:10: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 08:47:10: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 08:47:10: #1 total tags in treatment: 32969570 INFO @ Fri, 10 Dec 2021 08:47:10: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:47:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:47:11: #1 tags after filtering in treatment: 32969570 INFO @ Fri, 10 Dec 2021 08:47:11: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:47:11: #1 finished! INFO @ Fri, 10 Dec 2021 08:47:11: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:47:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:47:14: #2 number of paired peaks: 24 WARNING @ Fri, 10 Dec 2021 08:47:14: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 08:47:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling