Job ID = 10166165 SRX = SRX7723703 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 51994999 spots for SRR11084659/SRR11084659.sra Written 51994999 spots for SRR11084659/SRR11084659.sra fastq に変換しました。 bowtie でマッピング中... Your job 10166636 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:29 51994999 reads; of these: 51994999 (100.00%) were unpaired; of these: 12755887 (24.53%) aligned 0 times 34575940 (66.50%) aligned exactly 1 time 4663172 (8.97%) aligned >1 times 75.47% overall alignment rate Time searching: 00:16:29 Overall time: 00:16:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6269542 / 39239112 = 0.1598 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:04:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:04:22: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:04:22: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:04:29: 1000000 INFO @ Thu, 08 Oct 2020 21:04:36: 2000000 INFO @ Thu, 08 Oct 2020 21:04:44: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:04:51: 4000000 INFO @ Thu, 08 Oct 2020 21:04:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:04:52: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:04:52: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:04:59: 5000000 INFO @ Thu, 08 Oct 2020 21:04:59: 1000000 INFO @ Thu, 08 Oct 2020 21:05:06: 2000000 INFO @ Thu, 08 Oct 2020 21:05:07: 6000000 INFO @ Thu, 08 Oct 2020 21:05:14: 3000000 INFO @ Thu, 08 Oct 2020 21:05:15: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:05:21: 4000000 INFO @ Thu, 08 Oct 2020 21:05:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:05:22: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:05:22: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:05:23: 8000000 INFO @ Thu, 08 Oct 2020 21:05:28: 5000000 INFO @ Thu, 08 Oct 2020 21:05:30: 1000000 INFO @ Thu, 08 Oct 2020 21:05:31: 9000000 INFO @ Thu, 08 Oct 2020 21:05:36: 6000000 INFO @ Thu, 08 Oct 2020 21:05:38: 2000000 INFO @ Thu, 08 Oct 2020 21:05:39: 10000000 INFO @ Thu, 08 Oct 2020 21:05:44: 7000000 INFO @ Thu, 08 Oct 2020 21:05:46: 3000000 INFO @ Thu, 08 Oct 2020 21:05:47: 11000000 INFO @ Thu, 08 Oct 2020 21:05:51: 8000000 INFO @ Thu, 08 Oct 2020 21:05:54: 4000000 INFO @ Thu, 08 Oct 2020 21:05:55: 12000000 INFO @ Thu, 08 Oct 2020 21:05:59: 9000000 INFO @ Thu, 08 Oct 2020 21:06:02: 5000000 INFO @ Thu, 08 Oct 2020 21:06:03: 13000000 INFO @ Thu, 08 Oct 2020 21:06:06: 10000000 INFO @ Thu, 08 Oct 2020 21:06:11: 6000000 INFO @ Thu, 08 Oct 2020 21:06:11: 14000000 INFO @ Thu, 08 Oct 2020 21:06:14: 11000000 INFO @ Thu, 08 Oct 2020 21:06:19: 15000000 INFO @ Thu, 08 Oct 2020 21:06:19: 7000000 INFO @ Thu, 08 Oct 2020 21:06:21: 12000000 INFO @ Thu, 08 Oct 2020 21:06:27: 16000000 INFO @ Thu, 08 Oct 2020 21:06:27: 8000000 INFO @ Thu, 08 Oct 2020 21:06:28: 13000000 INFO @ Thu, 08 Oct 2020 21:06:35: 17000000 INFO @ Thu, 08 Oct 2020 21:06:35: 9000000 INFO @ Thu, 08 Oct 2020 21:06:36: 14000000 INFO @ Thu, 08 Oct 2020 21:06:43: 18000000 INFO @ Thu, 08 Oct 2020 21:06:43: 15000000 INFO @ Thu, 08 Oct 2020 21:06:43: 10000000 INFO @ Thu, 08 Oct 2020 21:06:50: 16000000 INFO @ Thu, 08 Oct 2020 21:06:51: 19000000 INFO @ Thu, 08 Oct 2020 21:06:51: 11000000 INFO @ Thu, 08 Oct 2020 21:06:58: 17000000 INFO @ Thu, 08 Oct 2020 21:06:58: 20000000 INFO @ Thu, 08 Oct 2020 21:06:59: 12000000 INFO @ Thu, 08 Oct 2020 21:07:05: 18000000 INFO @ Thu, 08 Oct 2020 21:07:06: 21000000 INFO @ Thu, 08 Oct 2020 21:07:07: 13000000 INFO @ Thu, 08 Oct 2020 21:07:12: 19000000 INFO @ Thu, 08 Oct 2020 21:07:14: 22000000 INFO @ Thu, 08 Oct 2020 21:07:15: 14000000 INFO @ Thu, 08 Oct 2020 21:07:20: 20000000 INFO @ Thu, 08 Oct 2020 21:07:22: 23000000 INFO @ Thu, 08 Oct 2020 21:07:23: 15000000 INFO @ Thu, 08 Oct 2020 21:07:27: 21000000 INFO @ Thu, 08 Oct 2020 21:07:30: 24000000 INFO @ Thu, 08 Oct 2020 21:07:31: 16000000 INFO @ Thu, 08 Oct 2020 21:07:34: 22000000 INFO @ Thu, 08 Oct 2020 21:07:38: 25000000 INFO @ Thu, 08 Oct 2020 21:07:39: 17000000 INFO @ Thu, 08 Oct 2020 21:07:41: 23000000 INFO @ Thu, 08 Oct 2020 21:07:46: 26000000 INFO @ Thu, 08 Oct 2020 21:07:47: 18000000 INFO @ Thu, 08 Oct 2020 21:07:49: 24000000 INFO @ Thu, 08 Oct 2020 21:07:55: 27000000 INFO @ Thu, 08 Oct 2020 21:07:55: 19000000 INFO @ Thu, 08 Oct 2020 21:07:56: 25000000 INFO @ Thu, 08 Oct 2020 21:08:03: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 21:08:03: 20000000 INFO @ Thu, 08 Oct 2020 21:08:03: 26000000 INFO @ Thu, 08 Oct 2020 21:08:11: 27000000 INFO @ Thu, 08 Oct 2020 21:08:11: 29000000 INFO @ Thu, 08 Oct 2020 21:08:11: 21000000 INFO @ Thu, 08 Oct 2020 21:08:18: 28000000 INFO @ Thu, 08 Oct 2020 21:08:19: 22000000 INFO @ Thu, 08 Oct 2020 21:08:20: 30000000 INFO @ Thu, 08 Oct 2020 21:08:25: 29000000 INFO @ Thu, 08 Oct 2020 21:08:27: 23000000 INFO @ Thu, 08 Oct 2020 21:08:29: 31000000 INFO @ Thu, 08 Oct 2020 21:08:33: 30000000 INFO @ Thu, 08 Oct 2020 21:08:36: 24000000 INFO @ Thu, 08 Oct 2020 21:08:38: 32000000 INFO @ Thu, 08 Oct 2020 21:08:40: 31000000 INFO @ Thu, 08 Oct 2020 21:08:44: 25000000 INFO @ Thu, 08 Oct 2020 21:08:46: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:08:46: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:08:46: #1 total tags in treatment: 32969570 INFO @ Thu, 08 Oct 2020 21:08:46: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:08:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:08:47: #1 tags after filtering in treatment: 32969570 INFO @ Thu, 08 Oct 2020 21:08:47: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:08:47: #1 finished! INFO @ Thu, 08 Oct 2020 21:08:47: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:08:47: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:08:47: 32000000 INFO @ Thu, 08 Oct 2020 21:08:49: #2 number of paired peaks: 24 WARNING @ Thu, 08 Oct 2020 21:08:49: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:08:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 21:08:52: 26000000 INFO @ Thu, 08 Oct 2020 21:08:55: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:08:55: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:08:55: #1 total tags in treatment: 32969570 INFO @ Thu, 08 Oct 2020 21:08:55: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:08:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:08:55: #1 tags after filtering in treatment: 32969570 INFO @ Thu, 08 Oct 2020 21:08:55: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:08:55: #1 finished! INFO @ Thu, 08 Oct 2020 21:08:55: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:08:55: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:08:57: #2 number of paired peaks: 24 WARNING @ Thu, 08 Oct 2020 21:08:57: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:08:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 21:08:59: 27000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 21:09:07: 28000000 INFO @ Thu, 08 Oct 2020 21:09:14: 29000000 INFO @ Thu, 08 Oct 2020 21:09:22: 30000000 INFO @ Thu, 08 Oct 2020 21:09:30: 31000000 INFO @ Thu, 08 Oct 2020 21:09:37: 32000000 INFO @ Thu, 08 Oct 2020 21:09:45: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 21:09:45: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 21:09:45: #1 total tags in treatment: 32969570 INFO @ Thu, 08 Oct 2020 21:09:45: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 21:09:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 21:09:45: #1 tags after filtering in treatment: 32969570 INFO @ Thu, 08 Oct 2020 21:09:45: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 21:09:45: #1 finished! INFO @ Thu, 08 Oct 2020 21:09:45: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 21:09:45: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 21:09:47: #2 number of paired peaks: 24 WARNING @ Thu, 08 Oct 2020 21:09:47: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 21:09:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723703/SRX7723703.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling