Job ID = 14167091 SRX = SRX7723693 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 33753233 spots for SRR11084649/SRR11084649.sra Written 33753233 spots for SRR11084649/SRR11084649.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167534 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:07 33753233 reads; of these: 33753233 (100.00%) were unpaired; of these: 4494605 (13.32%) aligned 0 times 24997694 (74.06%) aligned exactly 1 time 4260934 (12.62%) aligned >1 times 86.68% overall alignment rate Time searching: 00:17:07 Overall time: 00:17:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4499454 / 29258628 = 0.1538 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:16:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:16:30: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:16:30: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:16:41: 1000000 INFO @ Fri, 10 Dec 2021 10:16:51: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:17:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:17:00: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:17:00: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:17:02: 3000000 INFO @ Fri, 10 Dec 2021 10:17:12: 1000000 INFO @ Fri, 10 Dec 2021 10:17:13: 4000000 INFO @ Fri, 10 Dec 2021 10:17:24: 5000000 INFO @ Fri, 10 Dec 2021 10:17:24: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 10:17:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 10:17:30: #1 read tag files... INFO @ Fri, 10 Dec 2021 10:17:30: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 10:17:35: 6000000 INFO @ Fri, 10 Dec 2021 10:17:36: 3000000 INFO @ Fri, 10 Dec 2021 10:17:43: 1000000 INFO @ Fri, 10 Dec 2021 10:17:47: 7000000 INFO @ Fri, 10 Dec 2021 10:17:49: 4000000 INFO @ Fri, 10 Dec 2021 10:17:55: 2000000 INFO @ Fri, 10 Dec 2021 10:17:58: 8000000 INFO @ Fri, 10 Dec 2021 10:18:01: 5000000 INFO @ Fri, 10 Dec 2021 10:18:08: 3000000 INFO @ Fri, 10 Dec 2021 10:18:10: 9000000 INFO @ Fri, 10 Dec 2021 10:18:14: 6000000 INFO @ Fri, 10 Dec 2021 10:18:21: 4000000 INFO @ Fri, 10 Dec 2021 10:18:21: 10000000 INFO @ Fri, 10 Dec 2021 10:18:26: 7000000 INFO @ Fri, 10 Dec 2021 10:18:33: 11000000 INFO @ Fri, 10 Dec 2021 10:18:33: 5000000 INFO @ Fri, 10 Dec 2021 10:18:39: 8000000 INFO @ Fri, 10 Dec 2021 10:18:45: 12000000 INFO @ Fri, 10 Dec 2021 10:18:46: 6000000 INFO @ Fri, 10 Dec 2021 10:18:51: 9000000 INFO @ Fri, 10 Dec 2021 10:18:56: 13000000 INFO @ Fri, 10 Dec 2021 10:18:58: 7000000 INFO @ Fri, 10 Dec 2021 10:19:04: 10000000 INFO @ Fri, 10 Dec 2021 10:19:08: 14000000 INFO @ Fri, 10 Dec 2021 10:19:10: 8000000 INFO @ Fri, 10 Dec 2021 10:19:17: 11000000 INFO @ Fri, 10 Dec 2021 10:19:19: 15000000 INFO @ Fri, 10 Dec 2021 10:19:22: 9000000 INFO @ Fri, 10 Dec 2021 10:19:29: 12000000 INFO @ Fri, 10 Dec 2021 10:19:31: 16000000 INFO @ Fri, 10 Dec 2021 10:19:35: 10000000 INFO @ Fri, 10 Dec 2021 10:19:42: 13000000 INFO @ Fri, 10 Dec 2021 10:19:42: 17000000 INFO @ Fri, 10 Dec 2021 10:19:47: 11000000 INFO @ Fri, 10 Dec 2021 10:19:53: 18000000 INFO @ Fri, 10 Dec 2021 10:19:53: 14000000 INFO @ Fri, 10 Dec 2021 10:20:00: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 10:20:05: 19000000 INFO @ Fri, 10 Dec 2021 10:20:05: 15000000 INFO @ Fri, 10 Dec 2021 10:20:13: 13000000 INFO @ Fri, 10 Dec 2021 10:20:16: 20000000 INFO @ Fri, 10 Dec 2021 10:20:18: 16000000 INFO @ Fri, 10 Dec 2021 10:20:24: 14000000 INFO @ Fri, 10 Dec 2021 10:20:28: 21000000 INFO @ Fri, 10 Dec 2021 10:20:29: 17000000 INFO @ Fri, 10 Dec 2021 10:20:35: 15000000 INFO @ Fri, 10 Dec 2021 10:20:39: 22000000 INFO @ Fri, 10 Dec 2021 10:20:41: 18000000 INFO @ Fri, 10 Dec 2021 10:20:47: 16000000 INFO @ Fri, 10 Dec 2021 10:20:50: 23000000 INFO @ Fri, 10 Dec 2021 10:20:53: 19000000 INFO @ Fri, 10 Dec 2021 10:20:59: 17000000 INFO @ Fri, 10 Dec 2021 10:21:00: 24000000 INFO @ Fri, 10 Dec 2021 10:21:05: 20000000 INFO @ Fri, 10 Dec 2021 10:21:09: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:21:09: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:21:09: #1 total tags in treatment: 24759174 INFO @ Fri, 10 Dec 2021 10:21:09: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:21:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:21:09: #1 tags after filtering in treatment: 24759174 INFO @ Fri, 10 Dec 2021 10:21:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:21:09: #1 finished! INFO @ Fri, 10 Dec 2021 10:21:09: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:21:09: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:21:10: 18000000 INFO @ Fri, 10 Dec 2021 10:21:11: #2 number of paired peaks: 29 WARNING @ Fri, 10 Dec 2021 10:21:11: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:21:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 10:21:16: 21000000 INFO @ Fri, 10 Dec 2021 10:21:22: 19000000 INFO @ Fri, 10 Dec 2021 10:21:28: 22000000 INFO @ Fri, 10 Dec 2021 10:21:33: 20000000 INFO @ Fri, 10 Dec 2021 10:21:40: 23000000 INFO @ Fri, 10 Dec 2021 10:21:45: 21000000 INFO @ Fri, 10 Dec 2021 10:21:51: 24000000 INFO @ Fri, 10 Dec 2021 10:21:57: 22000000 INFO @ Fri, 10 Dec 2021 10:22:00: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:22:00: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:22:00: #1 total tags in treatment: 24759174 INFO @ Fri, 10 Dec 2021 10:22:00: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:22:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:22:00: #1 tags after filtering in treatment: 24759174 INFO @ Fri, 10 Dec 2021 10:22:00: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:22:00: #1 finished! INFO @ Fri, 10 Dec 2021 10:22:00: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:22:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:22:02: #2 number of paired peaks: 29 WARNING @ Fri, 10 Dec 2021 10:22:02: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:22:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 10:22:08: 23000000 INFO @ Fri, 10 Dec 2021 10:22:19: 24000000 INFO @ Fri, 10 Dec 2021 10:22:28: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 10:22:28: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 10:22:28: #1 total tags in treatment: 24759174 INFO @ Fri, 10 Dec 2021 10:22:28: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 10:22:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 10:22:28: #1 tags after filtering in treatment: 24759174 INFO @ Fri, 10 Dec 2021 10:22:28: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 10:22:28: #1 finished! INFO @ Fri, 10 Dec 2021 10:22:28: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 10:22:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 10:22:30: #2 number of paired peaks: 29 WARNING @ Fri, 10 Dec 2021 10:22:30: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 10:22:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling