Job ID = 10165848 SRX = SRX7723693 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 33753233 spots for SRR11084649/SRR11084649.sra Written 33753233 spots for SRR11084649/SRR11084649.sra fastq に変換しました。 bowtie でマッピング中... Your job 10166236 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:01 33753233 reads; of these: 33753233 (100.00%) were unpaired; of these: 4494605 (13.32%) aligned 0 times 24997694 (74.06%) aligned exactly 1 time 4260934 (12.62%) aligned >1 times 86.68% overall alignment rate Time searching: 00:12:01 Overall time: 00:12:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4499454 / 29258628 = 0.1538 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:23:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:23:02: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:23:02: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:23:11: 1000000 INFO @ Thu, 08 Oct 2020 20:23:19: 2000000 INFO @ Thu, 08 Oct 2020 20:23:28: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:23:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:23:32: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:23:32: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:23:36: 4000000 INFO @ Thu, 08 Oct 2020 20:23:41: 1000000 INFO @ Thu, 08 Oct 2020 20:23:45: 5000000 INFO @ Thu, 08 Oct 2020 20:23:50: 2000000 INFO @ Thu, 08 Oct 2020 20:23:53: 6000000 INFO @ Thu, 08 Oct 2020 20:23:59: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:24:01: 7000000 INFO @ Thu, 08 Oct 2020 20:24:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:24:02: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:24:02: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:24:07: 4000000 INFO @ Thu, 08 Oct 2020 20:24:09: 8000000 INFO @ Thu, 08 Oct 2020 20:24:11: 1000000 INFO @ Thu, 08 Oct 2020 20:24:16: 5000000 INFO @ Thu, 08 Oct 2020 20:24:18: 9000000 INFO @ Thu, 08 Oct 2020 20:24:19: 2000000 INFO @ Thu, 08 Oct 2020 20:24:24: 6000000 INFO @ Thu, 08 Oct 2020 20:24:26: 10000000 INFO @ Thu, 08 Oct 2020 20:24:28: 3000000 INFO @ Thu, 08 Oct 2020 20:24:33: 7000000 INFO @ Thu, 08 Oct 2020 20:24:34: 11000000 INFO @ Thu, 08 Oct 2020 20:24:36: 4000000 INFO @ Thu, 08 Oct 2020 20:24:42: 8000000 INFO @ Thu, 08 Oct 2020 20:24:42: 12000000 INFO @ Thu, 08 Oct 2020 20:24:44: 5000000 INFO @ Thu, 08 Oct 2020 20:24:50: 9000000 INFO @ Thu, 08 Oct 2020 20:24:51: 13000000 INFO @ Thu, 08 Oct 2020 20:24:52: 6000000 INFO @ Thu, 08 Oct 2020 20:24:59: 10000000 INFO @ Thu, 08 Oct 2020 20:24:59: 14000000 INFO @ Thu, 08 Oct 2020 20:25:01: 7000000 INFO @ Thu, 08 Oct 2020 20:25:07: 15000000 INFO @ Thu, 08 Oct 2020 20:25:07: 11000000 INFO @ Thu, 08 Oct 2020 20:25:09: 8000000 INFO @ Thu, 08 Oct 2020 20:25:15: 16000000 INFO @ Thu, 08 Oct 2020 20:25:16: 12000000 INFO @ Thu, 08 Oct 2020 20:25:17: 9000000 INFO @ Thu, 08 Oct 2020 20:25:23: 17000000 INFO @ Thu, 08 Oct 2020 20:25:24: 13000000 INFO @ Thu, 08 Oct 2020 20:25:25: 10000000 INFO @ Thu, 08 Oct 2020 20:25:31: 18000000 INFO @ Thu, 08 Oct 2020 20:25:33: 14000000 INFO @ Thu, 08 Oct 2020 20:25:33: 11000000 INFO @ Thu, 08 Oct 2020 20:25:39: 19000000 INFO @ Thu, 08 Oct 2020 20:25:41: 15000000 INFO @ Thu, 08 Oct 2020 20:25:41: 12000000 INFO @ Thu, 08 Oct 2020 20:25:47: 20000000 INFO @ Thu, 08 Oct 2020 20:25:50: 13000000 INFO @ Thu, 08 Oct 2020 20:25:50: 16000000 INFO @ Thu, 08 Oct 2020 20:25:55: 21000000 INFO @ Thu, 08 Oct 2020 20:25:58: 14000000 INFO @ Thu, 08 Oct 2020 20:25:58: 17000000 INFO @ Thu, 08 Oct 2020 20:26:04: 22000000 INFO @ Thu, 08 Oct 2020 20:26:06: 15000000 INFO @ Thu, 08 Oct 2020 20:26:07: 18000000 INFO @ Thu, 08 Oct 2020 20:26:12: 23000000 INFO @ Thu, 08 Oct 2020 20:26:14: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:26:15: 19000000 INFO @ Thu, 08 Oct 2020 20:26:20: 24000000 INFO @ Thu, 08 Oct 2020 20:26:22: 17000000 INFO @ Thu, 08 Oct 2020 20:26:24: 20000000 INFO @ Thu, 08 Oct 2020 20:26:26: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 20:26:26: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 20:26:26: #1 total tags in treatment: 24759174 INFO @ Thu, 08 Oct 2020 20:26:26: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:26:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:26:26: #1 tags after filtering in treatment: 24759174 INFO @ Thu, 08 Oct 2020 20:26:26: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:26:26: #1 finished! INFO @ Thu, 08 Oct 2020 20:26:26: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:26:26: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:26:28: #2 number of paired peaks: 29 WARNING @ Thu, 08 Oct 2020 20:26:28: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:26:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:26:30: 18000000 INFO @ Thu, 08 Oct 2020 20:26:32: 21000000 INFO @ Thu, 08 Oct 2020 20:26:38: 19000000 INFO @ Thu, 08 Oct 2020 20:26:40: 22000000 INFO @ Thu, 08 Oct 2020 20:26:46: 20000000 INFO @ Thu, 08 Oct 2020 20:26:49: 23000000 INFO @ Thu, 08 Oct 2020 20:26:54: 21000000 INFO @ Thu, 08 Oct 2020 20:26:57: 24000000 INFO @ Thu, 08 Oct 2020 20:27:02: 22000000 INFO @ Thu, 08 Oct 2020 20:27:04: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 20:27:04: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 20:27:04: #1 total tags in treatment: 24759174 INFO @ Thu, 08 Oct 2020 20:27:04: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:27:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:27:04: #1 tags after filtering in treatment: 24759174 INFO @ Thu, 08 Oct 2020 20:27:04: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:27:04: #1 finished! INFO @ Thu, 08 Oct 2020 20:27:04: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:27:04: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:27:06: #2 number of paired peaks: 29 WARNING @ Thu, 08 Oct 2020 20:27:06: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:27:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:27:11: 23000000 INFO @ Thu, 08 Oct 2020 20:27:19: 24000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 20:27:25: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 20:27:25: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 20:27:25: #1 total tags in treatment: 24759174 INFO @ Thu, 08 Oct 2020 20:27:25: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:27:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:27:26: #1 tags after filtering in treatment: 24759174 INFO @ Thu, 08 Oct 2020 20:27:26: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:27:26: #1 finished! INFO @ Thu, 08 Oct 2020 20:27:26: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:27:26: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:27:27: #2 number of paired peaks: 29 WARNING @ Thu, 08 Oct 2020 20:27:27: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:27:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7723693/SRX7723693.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling