Job ID = 6528500 SRX = SRX7672889 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:34:58 prefetch.2.10.7: 1) Downloading 'SRR11017736'... 2020-06-29T15:34:58 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:37:35 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:37:35 prefetch.2.10.7: 1) 'SRR11017736' was downloaded successfully Read 20092910 spots for SRR11017736/SRR11017736.sra Written 20092910 spots for SRR11017736/SRR11017736.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:52 20092910 reads; of these: 20092910 (100.00%) were unpaired; of these: 2402117 (11.96%) aligned 0 times 12585100 (62.63%) aligned exactly 1 time 5105693 (25.41%) aligned >1 times 88.04% overall alignment rate Time searching: 00:06:52 Overall time: 00:06:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1519662 / 17690793 = 0.0859 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:58:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:58:10: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:58:10: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:58:16: 1000000 INFO @ Tue, 30 Jun 2020 00:58:22: 2000000 INFO @ Tue, 30 Jun 2020 00:58:27: 3000000 INFO @ Tue, 30 Jun 2020 00:58:32: 4000000 INFO @ Tue, 30 Jun 2020 00:58:38: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:58:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:58:40: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:58:40: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:58:43: 6000000 INFO @ Tue, 30 Jun 2020 00:58:47: 1000000 INFO @ Tue, 30 Jun 2020 00:58:49: 7000000 INFO @ Tue, 30 Jun 2020 00:58:53: 2000000 INFO @ Tue, 30 Jun 2020 00:58:55: 8000000 INFO @ Tue, 30 Jun 2020 00:58:59: 3000000 INFO @ Tue, 30 Jun 2020 00:59:01: 9000000 INFO @ Tue, 30 Jun 2020 00:59:05: 4000000 INFO @ Tue, 30 Jun 2020 00:59:07: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:59:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:59:10: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:59:10: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:59:11: 5000000 INFO @ Tue, 30 Jun 2020 00:59:13: 11000000 INFO @ Tue, 30 Jun 2020 00:59:16: 1000000 INFO @ Tue, 30 Jun 2020 00:59:17: 6000000 INFO @ Tue, 30 Jun 2020 00:59:19: 12000000 INFO @ Tue, 30 Jun 2020 00:59:22: 2000000 INFO @ Tue, 30 Jun 2020 00:59:23: 7000000 INFO @ Tue, 30 Jun 2020 00:59:26: 13000000 INFO @ Tue, 30 Jun 2020 00:59:27: 3000000 INFO @ Tue, 30 Jun 2020 00:59:29: 8000000 INFO @ Tue, 30 Jun 2020 00:59:32: 14000000 INFO @ Tue, 30 Jun 2020 00:59:33: 4000000 INFO @ Tue, 30 Jun 2020 00:59:35: 9000000 INFO @ Tue, 30 Jun 2020 00:59:38: 15000000 INFO @ Tue, 30 Jun 2020 00:59:38: 5000000 INFO @ Tue, 30 Jun 2020 00:59:41: 10000000 INFO @ Tue, 30 Jun 2020 00:59:44: 6000000 INFO @ Tue, 30 Jun 2020 00:59:44: 16000000 INFO @ Tue, 30 Jun 2020 00:59:45: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:59:45: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:59:45: #1 total tags in treatment: 16171131 INFO @ Tue, 30 Jun 2020 00:59:45: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:59:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:59:45: #1 tags after filtering in treatment: 16171131 INFO @ Tue, 30 Jun 2020 00:59:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:59:45: #1 finished! INFO @ Tue, 30 Jun 2020 00:59:45: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:59:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:59:46: #2 number of paired peaks: 87 WARNING @ Tue, 30 Jun 2020 00:59:46: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:59:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:59:47: 11000000 INFO @ Tue, 30 Jun 2020 00:59:49: 7000000 INFO @ Tue, 30 Jun 2020 00:59:53: 12000000 INFO @ Tue, 30 Jun 2020 00:59:55: 8000000 INFO @ Tue, 30 Jun 2020 00:59:58: 13000000 INFO @ Tue, 30 Jun 2020 01:00:01: 9000000 INFO @ Tue, 30 Jun 2020 01:00:04: 14000000 INFO @ Tue, 30 Jun 2020 01:00:06: 10000000 INFO @ Tue, 30 Jun 2020 01:00:09: 15000000 INFO @ Tue, 30 Jun 2020 01:00:12: 11000000 INFO @ Tue, 30 Jun 2020 01:00:16: 16000000 INFO @ Tue, 30 Jun 2020 01:00:17: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 01:00:17: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 01:00:17: #1 total tags in treatment: 16171131 INFO @ Tue, 30 Jun 2020 01:00:17: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:00:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:00:17: #1 tags after filtering in treatment: 16171131 INFO @ Tue, 30 Jun 2020 01:00:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:00:17: #1 finished! INFO @ Tue, 30 Jun 2020 01:00:17: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:00:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:00:17: 12000000 INFO @ Tue, 30 Jun 2020 01:00:18: #2 number of paired peaks: 87 WARNING @ Tue, 30 Jun 2020 01:00:18: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:00:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:00:23: 13000000 INFO @ Tue, 30 Jun 2020 01:00:29: 14000000 INFO @ Tue, 30 Jun 2020 01:00:34: 15000000 INFO @ Tue, 30 Jun 2020 01:00:39: 16000000 INFO @ Tue, 30 Jun 2020 01:00:40: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 01:00:40: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 01:00:40: #1 total tags in treatment: 16171131 INFO @ Tue, 30 Jun 2020 01:00:40: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:00:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:00:41: #1 tags after filtering in treatment: 16171131 INFO @ Tue, 30 Jun 2020 01:00:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:00:41: #1 finished! INFO @ Tue, 30 Jun 2020 01:00:41: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:00:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:00:42: #2 number of paired peaks: 87 WARNING @ Tue, 30 Jun 2020 01:00:42: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:00:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7672889/SRX7672889.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。