Job ID = 6528498 SRX = SRX7672887 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:10:20 prefetch.2.10.7: 1) Downloading 'SRR11017734'... 2020-06-29T15:10:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:15:37 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:15:37 prefetch.2.10.7: 1) 'SRR11017734' was downloaded successfully Read 25012710 spots for SRR11017734/SRR11017734.sra Written 25012710 spots for SRR11017734/SRR11017734.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:49 25012710 reads; of these: 25012710 (100.00%) were unpaired; of these: 3105986 (12.42%) aligned 0 times 15530296 (62.09%) aligned exactly 1 time 6376428 (25.49%) aligned >1 times 87.58% overall alignment rate Time searching: 00:08:49 Overall time: 00:08:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2245153 / 21906724 = 0.1025 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:39:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:39:03: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:39:03: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:39:08: 1000000 INFO @ Tue, 30 Jun 2020 00:39:14: 2000000 INFO @ Tue, 30 Jun 2020 00:39:20: 3000000 INFO @ Tue, 30 Jun 2020 00:39:26: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:39:32: 5000000 INFO @ Tue, 30 Jun 2020 00:39:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:39:33: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:39:33: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:39:38: 6000000 INFO @ Tue, 30 Jun 2020 00:39:39: 1000000 INFO @ Tue, 30 Jun 2020 00:39:45: 7000000 INFO @ Tue, 30 Jun 2020 00:39:45: 2000000 INFO @ Tue, 30 Jun 2020 00:39:51: 8000000 INFO @ Tue, 30 Jun 2020 00:39:52: 3000000 INFO @ Tue, 30 Jun 2020 00:39:58: 9000000 INFO @ Tue, 30 Jun 2020 00:39:58: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:40:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:40:03: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:40:03: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:40:04: 10000000 INFO @ Tue, 30 Jun 2020 00:40:05: 5000000 INFO @ Tue, 30 Jun 2020 00:40:09: 1000000 INFO @ Tue, 30 Jun 2020 00:40:11: 11000000 INFO @ Tue, 30 Jun 2020 00:40:11: 6000000 INFO @ Tue, 30 Jun 2020 00:40:16: 2000000 INFO @ Tue, 30 Jun 2020 00:40:17: 12000000 INFO @ Tue, 30 Jun 2020 00:40:18: 7000000 INFO @ Tue, 30 Jun 2020 00:40:22: 3000000 INFO @ Tue, 30 Jun 2020 00:40:24: 13000000 INFO @ Tue, 30 Jun 2020 00:40:24: 8000000 INFO @ Tue, 30 Jun 2020 00:40:29: 4000000 INFO @ Tue, 30 Jun 2020 00:40:30: 14000000 INFO @ Tue, 30 Jun 2020 00:40:31: 9000000 INFO @ Tue, 30 Jun 2020 00:40:35: 5000000 INFO @ Tue, 30 Jun 2020 00:40:37: 15000000 INFO @ Tue, 30 Jun 2020 00:40:37: 10000000 INFO @ Tue, 30 Jun 2020 00:40:42: 6000000 INFO @ Tue, 30 Jun 2020 00:40:43: 16000000 INFO @ Tue, 30 Jun 2020 00:40:44: 11000000 INFO @ Tue, 30 Jun 2020 00:40:48: 7000000 INFO @ Tue, 30 Jun 2020 00:40:49: 17000000 INFO @ Tue, 30 Jun 2020 00:40:50: 12000000 INFO @ Tue, 30 Jun 2020 00:40:54: 8000000 INFO @ Tue, 30 Jun 2020 00:40:55: 18000000 INFO @ Tue, 30 Jun 2020 00:40:57: 13000000 INFO @ Tue, 30 Jun 2020 00:41:01: 9000000 INFO @ Tue, 30 Jun 2020 00:41:02: 19000000 INFO @ Tue, 30 Jun 2020 00:41:03: 14000000 INFO @ Tue, 30 Jun 2020 00:41:06: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:41:06: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:41:06: #1 total tags in treatment: 19661571 INFO @ Tue, 30 Jun 2020 00:41:06: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:41:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:41:06: #1 tags after filtering in treatment: 19661571 INFO @ Tue, 30 Jun 2020 00:41:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:41:06: #1 finished! INFO @ Tue, 30 Jun 2020 00:41:06: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:41:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:41:07: 10000000 INFO @ Tue, 30 Jun 2020 00:41:07: #2 number of paired peaks: 86 WARNING @ Tue, 30 Jun 2020 00:41:07: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:41:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:41:09: 15000000 INFO @ Tue, 30 Jun 2020 00:41:13: 11000000 INFO @ Tue, 30 Jun 2020 00:41:16: 16000000 INFO @ Tue, 30 Jun 2020 00:41:20: 12000000 INFO @ Tue, 30 Jun 2020 00:41:22: 17000000 INFO @ Tue, 30 Jun 2020 00:41:26: 13000000 INFO @ Tue, 30 Jun 2020 00:41:29: 18000000 INFO @ Tue, 30 Jun 2020 00:41:32: 14000000 INFO @ Tue, 30 Jun 2020 00:41:35: 19000000 INFO @ Tue, 30 Jun 2020 00:41:39: 15000000 INFO @ Tue, 30 Jun 2020 00:41:39: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:41:39: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:41:39: #1 total tags in treatment: 19661571 INFO @ Tue, 30 Jun 2020 00:41:39: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:41:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:41:40: #1 tags after filtering in treatment: 19661571 INFO @ Tue, 30 Jun 2020 00:41:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:41:40: #1 finished! INFO @ Tue, 30 Jun 2020 00:41:40: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:41:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:41:41: #2 number of paired peaks: 86 WARNING @ Tue, 30 Jun 2020 00:41:41: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:41:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:41:45: 16000000 INFO @ Tue, 30 Jun 2020 00:41:51: 17000000 INFO @ Tue, 30 Jun 2020 00:41:57: 18000000 INFO @ Tue, 30 Jun 2020 00:42:03: 19000000 INFO @ Tue, 30 Jun 2020 00:42:07: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:42:07: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:42:07: #1 total tags in treatment: 19661571 INFO @ Tue, 30 Jun 2020 00:42:07: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:42:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:42:07: #1 tags after filtering in treatment: 19661571 INFO @ Tue, 30 Jun 2020 00:42:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:42:07: #1 finished! INFO @ Tue, 30 Jun 2020 00:42:07: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:42:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:42:08: #2 number of paired peaks: 86 WARNING @ Tue, 30 Jun 2020 00:42:08: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:42:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7672887/SRX7672887.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。