Job ID = 6498782 SRX = SRX764277 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:58:49 prefetch.2.10.7: 1) Downloading 'SRR1657649'... 2020-06-25T23:58:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:00:22 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:00:22 prefetch.2.10.7: 1) 'SRR1657649' was downloaded successfully 2020-06-26T00:01:14 prefetch.2.10.7: 'SRR1657649' has 10 unresolved dependencies 2020-06-26T00:01:14 prefetch.2.10.7: 2) Downloading 'ncbi-acc:AE013599.4?vdb-ctx=refseq'... 2020-06-26T00:01:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:01:31 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:01:31 prefetch.2.10.7: 2) 'ncbi-acc:AE013599.4?vdb-ctx=refseq' was downloaded successfully 2020-06-26T00:01:31 prefetch.2.10.7: 3) Downloading 'ncbi-acc:AE014134.5?vdb-ctx=refseq'... 2020-06-26T00:01:31 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:01:49 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:01:49 prefetch.2.10.7: 3) 'ncbi-acc:AE014134.5?vdb-ctx=refseq' was downloaded successfully 2020-06-26T00:01:49 prefetch.2.10.7: 4) Downloading 'ncbi-acc:AE014135.3?vdb-ctx=refseq'... 2020-06-26T00:01:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:02:02 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:02:02 prefetch.2.10.7: 4) 'ncbi-acc:AE014135.3?vdb-ctx=refseq' was downloaded successfully 2020-06-26T00:02:02 prefetch.2.10.7: 5) Downloading 'ncbi-acc:AE014296.4?vdb-ctx=refseq'... 2020-06-26T00:02:02 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:02:28 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:02:28 prefetch.2.10.7: 5) 'ncbi-acc:AE014296.4?vdb-ctx=refseq' was downloaded successfully 2020-06-26T00:02:28 prefetch.2.10.7: 6) Downloading 'ncbi-acc:AE014297.2?vdb-ctx=refseq'... 2020-06-26T00:02:28 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:02:45 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:02:45 prefetch.2.10.7: 6) 'ncbi-acc:AE014297.2?vdb-ctx=refseq' was downloaded successfully 2020-06-26T00:02:45 prefetch.2.10.7: 7) Downloading 'ncbi-acc:AE014298.4?vdb-ctx=refseq'... 2020-06-26T00:02:45 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:03:01 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:03:01 prefetch.2.10.7: 7) 'ncbi-acc:AE014298.4?vdb-ctx=refseq' was downloaded successfully 2020-06-26T00:03:01 prefetch.2.10.7: 8) Downloading 'ncbi-acc:CM000457.1?vdb-ctx=refseq'... 2020-06-26T00:03:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:03:14 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:03:14 prefetch.2.10.7: 8) 'ncbi-acc:CM000457.1?vdb-ctx=refseq' was downloaded successfully 2020-06-26T00:03:14 prefetch.2.10.7: 9) Downloading 'ncbi-acc:CM000458.1?vdb-ctx=refseq'... 2020-06-26T00:03:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:03:27 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:03:27 prefetch.2.10.7: 9) 'ncbi-acc:CM000458.1?vdb-ctx=refseq' was downloaded successfully 2020-06-26T00:03:27 prefetch.2.10.7: 10) Downloading 'ncbi-acc:CM000459.1?vdb-ctx=refseq'... 2020-06-26T00:03:27 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:03:39 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:03:39 prefetch.2.10.7: 10) 'ncbi-acc:CM000459.1?vdb-ctx=refseq' was downloaded successfully 2020-06-26T00:03:39 prefetch.2.10.7: 11) Downloading 'ncbi-acc:FA000001.1?vdb-ctx=refseq'... 2020-06-26T00:03:39 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:04:05 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:04:05 prefetch.2.10.7: 11) 'ncbi-acc:FA000001.1?vdb-ctx=refseq' was downloaded successfully Read 18604196 spots for SRR1657649/SRR1657649.sra Written 18604196 spots for SRR1657649/SRR1657649.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:12 18604196 reads; of these: 18604196 (100.00%) were unpaired; of these: 3 (0.00%) aligned 0 times 18363014 (98.70%) aligned exactly 1 time 241179 (1.30%) aligned >1 times 100.00% overall alignment rate Time searching: 00:05:12 Overall time: 00:05:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 517 / 18604193 = 0.0000 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:18:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:18:52: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:18:52: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:18:59: 1000000 INFO @ Fri, 26 Jun 2020 09:19:06: 2000000 INFO @ Fri, 26 Jun 2020 09:19:13: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:19:21: 4000000 INFO @ Fri, 26 Jun 2020 09:19:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:19:22: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:19:22: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:19:28: 5000000 INFO @ Fri, 26 Jun 2020 09:19:30: 1000000 INFO @ Fri, 26 Jun 2020 09:19:36: 6000000 INFO @ Fri, 26 Jun 2020 09:19:38: 2000000 INFO @ Fri, 26 Jun 2020 09:19:44: 7000000 INFO @ Fri, 26 Jun 2020 09:19:46: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:19:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:19:52: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:19:52: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:19:52: 8000000 INFO @ Fri, 26 Jun 2020 09:19:54: 4000000 INFO @ Fri, 26 Jun 2020 09:20:01: 1000000 INFO @ Fri, 26 Jun 2020 09:20:01: 9000000 INFO @ Fri, 26 Jun 2020 09:20:03: 5000000 INFO @ Fri, 26 Jun 2020 09:20:10: 2000000 INFO @ Fri, 26 Jun 2020 09:20:10: 10000000 INFO @ Fri, 26 Jun 2020 09:20:11: 6000000 INFO @ Fri, 26 Jun 2020 09:20:19: 3000000 INFO @ Fri, 26 Jun 2020 09:20:19: 11000000 INFO @ Fri, 26 Jun 2020 09:20:20: 7000000 INFO @ Fri, 26 Jun 2020 09:20:27: 4000000 INFO @ Fri, 26 Jun 2020 09:20:27: 12000000 INFO @ Fri, 26 Jun 2020 09:20:29: 8000000 INFO @ Fri, 26 Jun 2020 09:20:36: 5000000 INFO @ Fri, 26 Jun 2020 09:20:36: 13000000 INFO @ Fri, 26 Jun 2020 09:20:38: 9000000 INFO @ Fri, 26 Jun 2020 09:20:44: 6000000 INFO @ Fri, 26 Jun 2020 09:20:45: 14000000 INFO @ Fri, 26 Jun 2020 09:20:46: 10000000 INFO @ Fri, 26 Jun 2020 09:20:53: 7000000 INFO @ Fri, 26 Jun 2020 09:20:54: 15000000 INFO @ Fri, 26 Jun 2020 09:20:55: 11000000 INFO @ Fri, 26 Jun 2020 09:21:02: 8000000 INFO @ Fri, 26 Jun 2020 09:21:03: 16000000 INFO @ Fri, 26 Jun 2020 09:21:04: 12000000 INFO @ Fri, 26 Jun 2020 09:21:10: 9000000 INFO @ Fri, 26 Jun 2020 09:21:12: 17000000 INFO @ Fri, 26 Jun 2020 09:21:13: 13000000 INFO @ Fri, 26 Jun 2020 09:21:19: 10000000 INFO @ Fri, 26 Jun 2020 09:21:20: 18000000 INFO @ Fri, 26 Jun 2020 09:21:21: 14000000 INFO @ Fri, 26 Jun 2020 09:21:26: #1 tag size is determined as 101 bps INFO @ Fri, 26 Jun 2020 09:21:26: #1 tag size = 101 INFO @ Fri, 26 Jun 2020 09:21:26: #1 total tags in treatment: 18603676 INFO @ Fri, 26 Jun 2020 09:21:26: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:21:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:21:26: #1 tags after filtering in treatment: 18603675 INFO @ Fri, 26 Jun 2020 09:21:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:21:26: #1 finished! INFO @ Fri, 26 Jun 2020 09:21:26: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:21:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:21:27: 11000000 INFO @ Fri, 26 Jun 2020 09:21:27: #2 number of paired peaks: 1 WARNING @ Fri, 26 Jun 2020 09:21:27: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:21:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:21:30: 15000000 INFO @ Fri, 26 Jun 2020 09:21:34: 12000000 INFO @ Fri, 26 Jun 2020 09:21:38: 16000000 INFO @ Fri, 26 Jun 2020 09:21:42: 13000000 INFO @ Fri, 26 Jun 2020 09:21:46: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 09:21:50: 14000000 INFO @ Fri, 26 Jun 2020 09:21:54: 18000000 INFO @ Fri, 26 Jun 2020 09:21:58: 15000000 INFO @ Fri, 26 Jun 2020 09:21:59: #1 tag size is determined as 101 bps INFO @ Fri, 26 Jun 2020 09:21:59: #1 tag size = 101 INFO @ Fri, 26 Jun 2020 09:21:59: #1 total tags in treatment: 18603676 INFO @ Fri, 26 Jun 2020 09:21:59: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:21:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:22:00: #1 tags after filtering in treatment: 18603675 INFO @ Fri, 26 Jun 2020 09:22:00: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:22:00: #1 finished! INFO @ Fri, 26 Jun 2020 09:22:00: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:22:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:22:01: #2 number of paired peaks: 1 WARNING @ Fri, 26 Jun 2020 09:22:01: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:22:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:22:05: 16000000 INFO @ Fri, 26 Jun 2020 09:22:12: 17000000 INFO @ Fri, 26 Jun 2020 09:22:19: 18000000 INFO @ Fri, 26 Jun 2020 09:22:24: #1 tag size is determined as 101 bps INFO @ Fri, 26 Jun 2020 09:22:24: #1 tag size = 101 INFO @ Fri, 26 Jun 2020 09:22:24: #1 total tags in treatment: 18603676 INFO @ Fri, 26 Jun 2020 09:22:24: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:22:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:22:24: #1 tags after filtering in treatment: 18603675 INFO @ Fri, 26 Jun 2020 09:22:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:22:24: #1 finished! INFO @ Fri, 26 Jun 2020 09:22:24: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:22:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:22:25: #2 number of paired peaks: 1 WARNING @ Fri, 26 Jun 2020 09:22:25: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:22:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX764277/SRX764277.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。