Job ID = 6528495 SRX = SRX760497 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:32:42 prefetch.2.10.7: 1) Downloading 'SRR1653728'... 2020-06-29T15:32:42 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:36:26 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:36:26 prefetch.2.10.7: 1) 'SRR1653728' was downloaded successfully 2020-06-29T15:36:26 prefetch.2.10.7: 'SRR1653728' has 0 unresolved dependencies Read 5983407 spots for SRR1653728/SRR1653728.sra Written 5983407 spots for SRR1653728/SRR1653728.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:42 5983407 reads; of these: 5983407 (100.00%) were unpaired; of these: 5983382 (100.00%) aligned 0 times 9 (0.00%) aligned exactly 1 time 16 (0.00%) aligned >1 times 0.00% overall alignment rate Time searching: 00:04:42 Overall time: 00:04:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 3 / 25 = 0.1200 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:48:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:48:05: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:48:05: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:48:05: #1 tag size is determined as 300 bps INFO @ Tue, 30 Jun 2020 00:48:05: #1 tag size = 300 INFO @ Tue, 30 Jun 2020 00:48:05: #1 total tags in treatment: 22 INFO @ Tue, 30 Jun 2020 00:48:05: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:48:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:48:05: #1 tags after filtering in treatment: 21 INFO @ Tue, 30 Jun 2020 00:48:05: #1 Redundant rate of treatment: 0.05 INFO @ Tue, 30 Jun 2020 00:48:05: #1 finished! INFO @ Tue, 30 Jun 2020 00:48:05: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:48:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:48:05: #2 number of paired peaks: 0 WARNING @ Tue, 30 Jun 2020 00:48:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:48:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:48:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:48:35: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:48:35: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:48:35: #1 tag size is determined as 300 bps INFO @ Tue, 30 Jun 2020 00:48:35: #1 tag size = 300 INFO @ Tue, 30 Jun 2020 00:48:35: #1 total tags in treatment: 22 INFO @ Tue, 30 Jun 2020 00:48:35: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:48:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:48:35: #1 tags after filtering in treatment: 21 INFO @ Tue, 30 Jun 2020 00:48:35: #1 Redundant rate of treatment: 0.05 INFO @ Tue, 30 Jun 2020 00:48:35: #1 finished! INFO @ Tue, 30 Jun 2020 00:48:35: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:48:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:48:35: #2 number of paired peaks: 0 WARNING @ Tue, 30 Jun 2020 00:48:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:48:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 00:49:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:49:05: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:49:05: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:49:05: #1 tag size is determined as 300 bps INFO @ Tue, 30 Jun 2020 00:49:05: #1 tag size = 300 INFO @ Tue, 30 Jun 2020 00:49:05: #1 total tags in treatment: 22 INFO @ Tue, 30 Jun 2020 00:49:05: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:49:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:49:05: #1 tags after filtering in treatment: 21 INFO @ Tue, 30 Jun 2020 00:49:05: #1 Redundant rate of treatment: 0.05 INFO @ Tue, 30 Jun 2020 00:49:05: #1 finished! INFO @ Tue, 30 Jun 2020 00:49:05: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:49:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:49:05: #2 number of paired peaks: 0 WARNING @ Tue, 30 Jun 2020 00:49:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:49:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760497/SRX760497.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling