Job ID = 6528489 SRX = SRX760385 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:13:21 prefetch.2.10.7: 1) Downloading 'SRR1653486'... 2020-06-29T15:13:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:16:41 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:16:41 prefetch.2.10.7: 1) 'SRR1653486' was downloaded successfully Read 30677615 spots for SRR1653486/SRR1653486.sra Written 30677615 spots for SRR1653486/SRR1653486.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:41 30677615 reads; of these: 30677615 (100.00%) were unpaired; of these: 1901922 (6.20%) aligned 0 times 21104839 (68.80%) aligned exactly 1 time 7670854 (25.00%) aligned >1 times 93.80% overall alignment rate Time searching: 00:10:41 Overall time: 00:10:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4616176 / 28775693 = 0.1604 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:42:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:42:21: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:42:21: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:42:26: 1000000 INFO @ Tue, 30 Jun 2020 00:42:31: 2000000 INFO @ Tue, 30 Jun 2020 00:42:37: 3000000 INFO @ Tue, 30 Jun 2020 00:42:42: 4000000 INFO @ Tue, 30 Jun 2020 00:42:47: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:42:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:42:51: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:42:51: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:42:52: 6000000 INFO @ Tue, 30 Jun 2020 00:42:58: 1000000 INFO @ Tue, 30 Jun 2020 00:42:58: 7000000 INFO @ Tue, 30 Jun 2020 00:43:04: 8000000 INFO @ Tue, 30 Jun 2020 00:43:05: 2000000 INFO @ Tue, 30 Jun 2020 00:43:10: 9000000 INFO @ Tue, 30 Jun 2020 00:43:11: 3000000 INFO @ Tue, 30 Jun 2020 00:43:15: 10000000 INFO @ Tue, 30 Jun 2020 00:43:18: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:43:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:43:21: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:43:21: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:43:21: 11000000 INFO @ Tue, 30 Jun 2020 00:43:25: 5000000 INFO @ Tue, 30 Jun 2020 00:43:27: 12000000 INFO @ Tue, 30 Jun 2020 00:43:27: 1000000 INFO @ Tue, 30 Jun 2020 00:43:31: 6000000 INFO @ Tue, 30 Jun 2020 00:43:33: 13000000 INFO @ Tue, 30 Jun 2020 00:43:33: 2000000 INFO @ Tue, 30 Jun 2020 00:43:38: 7000000 INFO @ Tue, 30 Jun 2020 00:43:39: 14000000 INFO @ Tue, 30 Jun 2020 00:43:39: 3000000 INFO @ Tue, 30 Jun 2020 00:43:44: 8000000 INFO @ Tue, 30 Jun 2020 00:43:45: 15000000 INFO @ Tue, 30 Jun 2020 00:43:45: 4000000 INFO @ Tue, 30 Jun 2020 00:43:50: 16000000 INFO @ Tue, 30 Jun 2020 00:43:51: 9000000 INFO @ Tue, 30 Jun 2020 00:43:51: 5000000 INFO @ Tue, 30 Jun 2020 00:43:56: 17000000 INFO @ Tue, 30 Jun 2020 00:43:57: 6000000 INFO @ Tue, 30 Jun 2020 00:43:57: 10000000 INFO @ Tue, 30 Jun 2020 00:44:02: 18000000 INFO @ Tue, 30 Jun 2020 00:44:03: 7000000 INFO @ Tue, 30 Jun 2020 00:44:04: 11000000 INFO @ Tue, 30 Jun 2020 00:44:08: 19000000 INFO @ Tue, 30 Jun 2020 00:44:09: 8000000 INFO @ Tue, 30 Jun 2020 00:44:11: 12000000 INFO @ Tue, 30 Jun 2020 00:44:14: 20000000 INFO @ Tue, 30 Jun 2020 00:44:15: 9000000 INFO @ Tue, 30 Jun 2020 00:44:17: 13000000 INFO @ Tue, 30 Jun 2020 00:44:21: 21000000 INFO @ Tue, 30 Jun 2020 00:44:21: 10000000 INFO @ Tue, 30 Jun 2020 00:44:25: 14000000 INFO @ Tue, 30 Jun 2020 00:44:27: 22000000 INFO @ Tue, 30 Jun 2020 00:44:27: 11000000 INFO @ Tue, 30 Jun 2020 00:44:31: 15000000 INFO @ Tue, 30 Jun 2020 00:44:33: 23000000 INFO @ Tue, 30 Jun 2020 00:44:33: 12000000 INFO @ Tue, 30 Jun 2020 00:44:38: 16000000 INFO @ Tue, 30 Jun 2020 00:44:39: 24000000 INFO @ Tue, 30 Jun 2020 00:44:39: 13000000 INFO @ Tue, 30 Jun 2020 00:44:40: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:44:40: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:44:40: #1 total tags in treatment: 24159517 INFO @ Tue, 30 Jun 2020 00:44:40: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:44:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:44:40: #1 tags after filtering in treatment: 24159517 INFO @ Tue, 30 Jun 2020 00:44:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:44:40: #1 finished! INFO @ Tue, 30 Jun 2020 00:44:40: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:44:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:44:41: #2 number of paired peaks: 36 WARNING @ Tue, 30 Jun 2020 00:44:41: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:44:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:44:44: 17000000 INFO @ Tue, 30 Jun 2020 00:44:45: 14000000 INFO @ Tue, 30 Jun 2020 00:44:51: 18000000 INFO @ Tue, 30 Jun 2020 00:44:51: 15000000 INFO @ Tue, 30 Jun 2020 00:44:57: 16000000 INFO @ Tue, 30 Jun 2020 00:44:57: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:45:03: 17000000 INFO @ Tue, 30 Jun 2020 00:45:04: 20000000 INFO @ Tue, 30 Jun 2020 00:45:09: 18000000 INFO @ Tue, 30 Jun 2020 00:45:11: 21000000 INFO @ Tue, 30 Jun 2020 00:45:15: 19000000 INFO @ Tue, 30 Jun 2020 00:45:18: 22000000 INFO @ Tue, 30 Jun 2020 00:45:21: 20000000 INFO @ Tue, 30 Jun 2020 00:45:25: 23000000 INFO @ Tue, 30 Jun 2020 00:45:27: 21000000 INFO @ Tue, 30 Jun 2020 00:45:31: 24000000 INFO @ Tue, 30 Jun 2020 00:45:32: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:45:32: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:45:32: #1 total tags in treatment: 24159517 INFO @ Tue, 30 Jun 2020 00:45:32: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:45:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:45:33: #1 tags after filtering in treatment: 24159517 INFO @ Tue, 30 Jun 2020 00:45:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:45:33: #1 finished! INFO @ Tue, 30 Jun 2020 00:45:33: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:45:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:45:33: 22000000 INFO @ Tue, 30 Jun 2020 00:45:34: #2 number of paired peaks: 36 WARNING @ Tue, 30 Jun 2020 00:45:34: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:45:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:45:39: 23000000 INFO @ Tue, 30 Jun 2020 00:45:44: 24000000 INFO @ Tue, 30 Jun 2020 00:45:45: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:45:45: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:45:45: #1 total tags in treatment: 24159517 INFO @ Tue, 30 Jun 2020 00:45:45: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:45:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:45:46: #1 tags after filtering in treatment: 24159517 INFO @ Tue, 30 Jun 2020 00:45:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:45:46: #1 finished! INFO @ Tue, 30 Jun 2020 00:45:46: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:45:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:45:47: #2 number of paired peaks: 36 WARNING @ Tue, 30 Jun 2020 00:45:47: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:45:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX760385/SRX760385.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。