Job ID = 12266639 SRX = SRX7572781 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 47610395 spots for SRR10904628/SRR10904628.sra Written 47610395 spots for SRR10904628/SRR10904628.sra fastq に変換しました。 bowtie でマッピング中... Your job 12271153 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:54:01 47610395 reads; of these: 47610395 (100.00%) were paired; of these: 21363602 (44.87%) aligned concordantly 0 times 18666552 (39.21%) aligned concordantly exactly 1 time 7580241 (15.92%) aligned concordantly >1 times ---- 21363602 pairs aligned concordantly 0 times; of these: 4804950 (22.49%) aligned discordantly 1 time ---- 16558652 pairs aligned 0 times concordantly or discordantly; of these: 33117304 mates make up the pairs; of these: 27180052 (82.07%) aligned 0 times 993809 (3.00%) aligned exactly 1 time 4943443 (14.93%) aligned >1 times 71.46% overall alignment rate Time searching: 02:54:01 Overall time: 02:54:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 52 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 9269234 / 30828990 = 0.3007 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 12:40:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 12:40:42: #1 read tag files... INFO @ Sat, 03 Apr 2021 12:40:42: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 12:40:49: 1000000 INFO @ Sat, 03 Apr 2021 12:40:57: 2000000 INFO @ Sat, 03 Apr 2021 12:41:04: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 12:41:11: 4000000 INFO @ Sat, 03 Apr 2021 12:41:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 12:41:12: #1 read tag files... INFO @ Sat, 03 Apr 2021 12:41:12: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 12:41:19: 5000000 INFO @ Sat, 03 Apr 2021 12:41:20: 1000000 INFO @ Sat, 03 Apr 2021 12:41:26: 6000000 INFO @ Sat, 03 Apr 2021 12:41:27: 2000000 INFO @ Sat, 03 Apr 2021 12:41:34: 7000000 INFO @ Sat, 03 Apr 2021 12:41:35: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 12:41:42: 8000000 INFO @ Sat, 03 Apr 2021 12:41:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 12:41:42: #1 read tag files... INFO @ Sat, 03 Apr 2021 12:41:42: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 12:41:43: 4000000 INFO @ Sat, 03 Apr 2021 12:41:50: 9000000 INFO @ Sat, 03 Apr 2021 12:41:51: 1000000 INFO @ Sat, 03 Apr 2021 12:41:52: 5000000 INFO @ Sat, 03 Apr 2021 12:41:59: 10000000 INFO @ Sat, 03 Apr 2021 12:42:00: 6000000 INFO @ Sat, 03 Apr 2021 12:42:00: 2000000 INFO @ Sat, 03 Apr 2021 12:42:08: 11000000 INFO @ Sat, 03 Apr 2021 12:42:09: 7000000 INFO @ Sat, 03 Apr 2021 12:42:09: 3000000 INFO @ Sat, 03 Apr 2021 12:42:17: 8000000 INFO @ Sat, 03 Apr 2021 12:42:18: 12000000 INFO @ Sat, 03 Apr 2021 12:42:18: 4000000 INFO @ Sat, 03 Apr 2021 12:42:25: 9000000 INFO @ Sat, 03 Apr 2021 12:42:27: 5000000 INFO @ Sat, 03 Apr 2021 12:42:27: 13000000 INFO @ Sat, 03 Apr 2021 12:42:34: 10000000 INFO @ Sat, 03 Apr 2021 12:42:35: 6000000 INFO @ Sat, 03 Apr 2021 12:42:36: 14000000 INFO @ Sat, 03 Apr 2021 12:42:43: 11000000 INFO @ Sat, 03 Apr 2021 12:42:44: 7000000 INFO @ Sat, 03 Apr 2021 12:42:45: 15000000 INFO @ Sat, 03 Apr 2021 12:42:52: 12000000 INFO @ Sat, 03 Apr 2021 12:42:52: 8000000 INFO @ Sat, 03 Apr 2021 12:42:54: 16000000 INFO @ Sat, 03 Apr 2021 12:43:01: 9000000 INFO @ Sat, 03 Apr 2021 12:43:01: 13000000 INFO @ Sat, 03 Apr 2021 12:43:03: 17000000 INFO @ Sat, 03 Apr 2021 12:43:09: 10000000 INFO @ Sat, 03 Apr 2021 12:43:09: 14000000 INFO @ Sat, 03 Apr 2021 12:43:12: 18000000 INFO @ Sat, 03 Apr 2021 12:43:18: 15000000 INFO @ Sat, 03 Apr 2021 12:43:18: 11000000 INFO @ Sat, 03 Apr 2021 12:43:21: 19000000 INFO @ Sat, 03 Apr 2021 12:43:27: 16000000 INFO @ Sat, 03 Apr 2021 12:43:27: 12000000 INFO @ Sat, 03 Apr 2021 12:43:30: 20000000 INFO @ Sat, 03 Apr 2021 12:43:36: 17000000 INFO @ Sat, 03 Apr 2021 12:43:36: 13000000 INFO @ Sat, 03 Apr 2021 12:43:39: 21000000 INFO @ Sat, 03 Apr 2021 12:43:45: 18000000 INFO @ Sat, 03 Apr 2021 12:43:45: 14000000 INFO @ Sat, 03 Apr 2021 12:43:48: 22000000 INFO @ Sat, 03 Apr 2021 12:43:53: 19000000 INFO @ Sat, 03 Apr 2021 12:43:53: 15000000 INFO @ Sat, 03 Apr 2021 12:43:58: 23000000 INFO @ Sat, 03 Apr 2021 12:44:02: 20000000 INFO @ Sat, 03 Apr 2021 12:44:02: 16000000 INFO @ Sat, 03 Apr 2021 12:44:07: 24000000 INFO @ Sat, 03 Apr 2021 12:44:11: 21000000 INFO @ Sat, 03 Apr 2021 12:44:11: 17000000 INFO @ Sat, 03 Apr 2021 12:44:16: 25000000 INFO @ Sat, 03 Apr 2021 12:44:19: 22000000 INFO @ Sat, 03 Apr 2021 12:44:19: 18000000 INFO @ Sat, 03 Apr 2021 12:44:24: 26000000 INFO @ Sat, 03 Apr 2021 12:44:27: 23000000 INFO @ Sat, 03 Apr 2021 12:44:27: 19000000 INFO @ Sat, 03 Apr 2021 12:44:33: 27000000 INFO @ Sat, 03 Apr 2021 12:44:35: 24000000 INFO @ Sat, 03 Apr 2021 12:44:35: 20000000 INFO @ Sat, 03 Apr 2021 12:44:40: 28000000 INFO @ Sat, 03 Apr 2021 12:44:42: 21000000 INFO @ Sat, 03 Apr 2021 12:44:43: 25000000 INFO @ Sat, 03 Apr 2021 12:44:47: 29000000 INFO @ Sat, 03 Apr 2021 12:44:50: 22000000 INFO @ Sat, 03 Apr 2021 12:44:51: 26000000 INFO @ Sat, 03 Apr 2021 12:44:55: 30000000 INFO @ Sat, 03 Apr 2021 12:44:58: 23000000 INFO @ Sat, 03 Apr 2021 12:44:58: 27000000 INFO @ Sat, 03 Apr 2021 12:45:02: 31000000 INFO @ Sat, 03 Apr 2021 12:45:05: 28000000 INFO @ Sat, 03 Apr 2021 12:45:06: 24000000 INFO @ Sat, 03 Apr 2021 12:45:09: 32000000 INFO @ Sat, 03 Apr 2021 12:45:13: 29000000 INFO @ Sat, 03 Apr 2021 12:45:14: 25000000 INFO @ Sat, 03 Apr 2021 12:45:17: 33000000 INFO @ Sat, 03 Apr 2021 12:45:21: 30000000 INFO @ Sat, 03 Apr 2021 12:45:22: 26000000 INFO @ Sat, 03 Apr 2021 12:45:24: 34000000 INFO @ Sat, 03 Apr 2021 12:45:28: 31000000 INFO @ Sat, 03 Apr 2021 12:45:29: 27000000 INFO @ Sat, 03 Apr 2021 12:45:32: 35000000 INFO @ Sat, 03 Apr 2021 12:45:36: 32000000 INFO @ Sat, 03 Apr 2021 12:45:37: 28000000 INFO @ Sat, 03 Apr 2021 12:45:39: 36000000 INFO @ Sat, 03 Apr 2021 12:45:44: 33000000 INFO @ Sat, 03 Apr 2021 12:45:45: 29000000 INFO @ Sat, 03 Apr 2021 12:45:46: 37000000 INFO @ Sat, 03 Apr 2021 12:45:51: 34000000 INFO @ Sat, 03 Apr 2021 12:45:53: 30000000 INFO @ Sat, 03 Apr 2021 12:45:54: 38000000 INFO @ Sat, 03 Apr 2021 12:45:58: 35000000 INFO @ Sat, 03 Apr 2021 12:46:01: 31000000 INFO @ Sat, 03 Apr 2021 12:46:02: 39000000 INFO @ Sat, 03 Apr 2021 12:46:06: 36000000 INFO @ Sat, 03 Apr 2021 12:46:08: 32000000 INFO @ Sat, 03 Apr 2021 12:46:10: 40000000 INFO @ Sat, 03 Apr 2021 12:46:16: 37000000 INFO @ Sat, 03 Apr 2021 12:46:16: 33000000 INFO @ Sat, 03 Apr 2021 12:46:19: 41000000 INFO @ Sat, 03 Apr 2021 12:46:24: 34000000 INFO @ Sat, 03 Apr 2021 12:46:24: 38000000 INFO @ Sat, 03 Apr 2021 12:46:27: 42000000 INFO @ Sat, 03 Apr 2021 12:46:31: 35000000 INFO @ Sat, 03 Apr 2021 12:46:31: 39000000 INFO @ Sat, 03 Apr 2021 12:46:34: 43000000 INFO @ Sat, 03 Apr 2021 12:46:39: 40000000 INFO @ Sat, 03 Apr 2021 12:46:39: 36000000 INFO @ Sat, 03 Apr 2021 12:46:42: 44000000 INFO @ Sat, 03 Apr 2021 12:46:46: 41000000 INFO @ Sat, 03 Apr 2021 12:46:46: 37000000 INFO @ Sat, 03 Apr 2021 12:46:50: 45000000 INFO @ Sat, 03 Apr 2021 12:46:53: 42000000 INFO @ Sat, 03 Apr 2021 12:46:54: 38000000 INFO @ Sat, 03 Apr 2021 12:46:57: 46000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 12:47:00: 43000000 INFO @ Sat, 03 Apr 2021 12:47:02: 39000000 INFO @ Sat, 03 Apr 2021 12:47:05: 47000000 INFO @ Sat, 03 Apr 2021 12:47:08: 44000000 INFO @ Sat, 03 Apr 2021 12:47:10: 40000000 INFO @ Sat, 03 Apr 2021 12:47:12: 48000000 INFO @ Sat, 03 Apr 2021 12:47:16: 45000000 INFO @ Sat, 03 Apr 2021 12:47:18: 41000000 INFO @ Sat, 03 Apr 2021 12:47:20: 49000000 INFO @ Sat, 03 Apr 2021 12:47:23: 46000000 INFO @ Sat, 03 Apr 2021 12:47:24: #1 tag size is determined as 151 bps INFO @ Sat, 03 Apr 2021 12:47:24: #1 tag size = 151 INFO @ Sat, 03 Apr 2021 12:47:24: #1 total tags in treatment: 18066420 INFO @ Sat, 03 Apr 2021 12:47:24: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 12:47:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 12:47:24: #1 tags after filtering in treatment: 14532106 INFO @ Sat, 03 Apr 2021 12:47:24: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 03 Apr 2021 12:47:24: #1 finished! INFO @ Sat, 03 Apr 2021 12:47:24: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 12:47:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 12:47:25: 42000000 INFO @ Sat, 03 Apr 2021 12:47:25: #2 number of paired peaks: 96 WARNING @ Sat, 03 Apr 2021 12:47:25: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 12:47:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 12:47:30: 47000000 INFO @ Sat, 03 Apr 2021 12:47:33: 43000000 INFO @ Sat, 03 Apr 2021 12:47:37: 48000000 INFO @ Sat, 03 Apr 2021 12:47:40: 44000000 INFO @ Sat, 03 Apr 2021 12:47:44: 49000000 INFO @ Sat, 03 Apr 2021 12:47:47: 45000000 INFO @ Sat, 03 Apr 2021 12:47:48: #1 tag size is determined as 151 bps INFO @ Sat, 03 Apr 2021 12:47:48: #1 tag size = 151 INFO @ Sat, 03 Apr 2021 12:47:48: #1 total tags in treatment: 18066420 INFO @ Sat, 03 Apr 2021 12:47:48: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 12:47:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 12:47:49: #1 tags after filtering in treatment: 14532106 INFO @ Sat, 03 Apr 2021 12:47:49: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 03 Apr 2021 12:47:49: #1 finished! INFO @ Sat, 03 Apr 2021 12:47:49: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 12:47:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 12:47:50: #2 number of paired peaks: 96 WARNING @ Sat, 03 Apr 2021 12:47:50: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 12:47:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 12:47:54: 46000000 INFO @ Sat, 03 Apr 2021 12:48:02: 47000000 INFO @ Sat, 03 Apr 2021 12:48:09: 48000000 INFO @ Sat, 03 Apr 2021 12:48:17: 49000000 INFO @ Sat, 03 Apr 2021 12:48:22: #1 tag size is determined as 151 bps INFO @ Sat, 03 Apr 2021 12:48:22: #1 tag size = 151 INFO @ Sat, 03 Apr 2021 12:48:22: #1 total tags in treatment: 18066420 INFO @ Sat, 03 Apr 2021 12:48:22: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 12:48:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 12:48:22: #1 tags after filtering in treatment: 14532106 INFO @ Sat, 03 Apr 2021 12:48:22: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 03 Apr 2021 12:48:22: #1 finished! INFO @ Sat, 03 Apr 2021 12:48:22: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 12:48:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 12:48:23: #2 number of paired peaks: 96 WARNING @ Sat, 03 Apr 2021 12:48:23: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 12:48:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7572781/SRX7572781.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。