Job ID = 12266635 SRX = SRX7572777 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 47284379 spots for SRR10904624/SRR10904624.sra Written 47284379 spots for SRR10904624/SRR10904624.sra fastq に変換しました。 bowtie でマッピング中... Your job 12269539 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:54:39 47284379 reads; of these: 47284379 (100.00%) were paired; of these: 21144226 (44.72%) aligned concordantly 0 times 21180412 (44.79%) aligned concordantly exactly 1 time 4959741 (10.49%) aligned concordantly >1 times ---- 21144226 pairs aligned concordantly 0 times; of these: 6519335 (30.83%) aligned discordantly 1 time ---- 14624891 pairs aligned 0 times concordantly or discordantly; of these: 29249782 mates make up the pairs; of these: 24636133 (84.23%) aligned 0 times 1280902 (4.38%) aligned exactly 1 time 3332747 (11.39%) aligned >1 times 73.95% overall alignment rate Time searching: 01:54:39 Overall time: 01:54:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 52 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 8105558 / 32506960 = 0.2493 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 11:33:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 11:33:36: #1 read tag files... INFO @ Sat, 03 Apr 2021 11:33:36: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 11:33:42: 1000000 INFO @ Sat, 03 Apr 2021 11:33:49: 2000000 INFO @ Sat, 03 Apr 2021 11:33:56: 3000000 INFO @ Sat, 03 Apr 2021 11:34:03: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 11:34:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 11:34:05: #1 read tag files... INFO @ Sat, 03 Apr 2021 11:34:05: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 11:34:10: 5000000 INFO @ Sat, 03 Apr 2021 11:34:13: 1000000 INFO @ Sat, 03 Apr 2021 11:34:17: 6000000 INFO @ Sat, 03 Apr 2021 11:34:20: 2000000 INFO @ Sat, 03 Apr 2021 11:34:24: 7000000 INFO @ Sat, 03 Apr 2021 11:34:27: 3000000 INFO @ Sat, 03 Apr 2021 11:34:31: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 11:34:35: 4000000 INFO @ Sat, 03 Apr 2021 11:34:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 11:34:36: #1 read tag files... INFO @ Sat, 03 Apr 2021 11:34:36: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 11:34:39: 9000000 INFO @ Sat, 03 Apr 2021 11:34:42: 5000000 INFO @ Sat, 03 Apr 2021 11:34:43: 1000000 INFO @ Sat, 03 Apr 2021 11:34:46: 10000000 INFO @ Sat, 03 Apr 2021 11:34:50: 6000000 INFO @ Sat, 03 Apr 2021 11:34:51: 2000000 INFO @ Sat, 03 Apr 2021 11:34:53: 11000000 INFO @ Sat, 03 Apr 2021 11:34:57: 7000000 INFO @ Sat, 03 Apr 2021 11:34:58: 3000000 INFO @ Sat, 03 Apr 2021 11:35:01: 12000000 INFO @ Sat, 03 Apr 2021 11:35:04: 8000000 INFO @ Sat, 03 Apr 2021 11:35:06: 4000000 INFO @ Sat, 03 Apr 2021 11:35:08: 13000000 INFO @ Sat, 03 Apr 2021 11:35:12: 9000000 INFO @ Sat, 03 Apr 2021 11:35:13: 5000000 INFO @ Sat, 03 Apr 2021 11:35:16: 14000000 INFO @ Sat, 03 Apr 2021 11:35:19: 10000000 INFO @ Sat, 03 Apr 2021 11:35:21: 6000000 INFO @ Sat, 03 Apr 2021 11:35:23: 15000000 INFO @ Sat, 03 Apr 2021 11:35:27: 11000000 INFO @ Sat, 03 Apr 2021 11:35:28: 7000000 INFO @ Sat, 03 Apr 2021 11:35:31: 16000000 INFO @ Sat, 03 Apr 2021 11:35:35: 12000000 INFO @ Sat, 03 Apr 2021 11:35:35: 8000000 INFO @ Sat, 03 Apr 2021 11:35:38: 17000000 INFO @ Sat, 03 Apr 2021 11:35:42: 13000000 INFO @ Sat, 03 Apr 2021 11:35:42: 9000000 INFO @ Sat, 03 Apr 2021 11:35:45: 18000000 INFO @ Sat, 03 Apr 2021 11:35:49: 14000000 INFO @ Sat, 03 Apr 2021 11:35:50: 10000000 INFO @ Sat, 03 Apr 2021 11:35:52: 19000000 INFO @ Sat, 03 Apr 2021 11:35:57: 15000000 INFO @ Sat, 03 Apr 2021 11:35:57: 11000000 INFO @ Sat, 03 Apr 2021 11:36:00: 20000000 INFO @ Sat, 03 Apr 2021 11:36:04: 16000000 INFO @ Sat, 03 Apr 2021 11:36:05: 12000000 INFO @ Sat, 03 Apr 2021 11:36:07: 21000000 INFO @ Sat, 03 Apr 2021 11:36:11: 17000000 INFO @ Sat, 03 Apr 2021 11:36:12: 13000000 INFO @ Sat, 03 Apr 2021 11:36:15: 22000000 INFO @ Sat, 03 Apr 2021 11:36:19: 18000000 INFO @ Sat, 03 Apr 2021 11:36:20: 14000000 INFO @ Sat, 03 Apr 2021 11:36:22: 23000000 INFO @ Sat, 03 Apr 2021 11:36:26: 19000000 INFO @ Sat, 03 Apr 2021 11:36:27: 15000000 INFO @ Sat, 03 Apr 2021 11:36:29: 24000000 INFO @ Sat, 03 Apr 2021 11:36:33: 20000000 INFO @ Sat, 03 Apr 2021 11:36:35: 16000000 INFO @ Sat, 03 Apr 2021 11:36:36: 25000000 INFO @ Sat, 03 Apr 2021 11:36:41: 21000000 INFO @ Sat, 03 Apr 2021 11:36:42: 17000000 INFO @ Sat, 03 Apr 2021 11:36:44: 26000000 INFO @ Sat, 03 Apr 2021 11:36:48: 22000000 INFO @ Sat, 03 Apr 2021 11:36:49: 18000000 INFO @ Sat, 03 Apr 2021 11:36:51: 27000000 INFO @ Sat, 03 Apr 2021 11:36:56: 23000000 INFO @ Sat, 03 Apr 2021 11:36:56: 19000000 INFO @ Sat, 03 Apr 2021 11:36:58: 28000000 INFO @ Sat, 03 Apr 2021 11:37:03: 24000000 INFO @ Sat, 03 Apr 2021 11:37:04: 20000000 INFO @ Sat, 03 Apr 2021 11:37:05: 29000000 INFO @ Sat, 03 Apr 2021 11:37:10: 25000000 INFO @ Sat, 03 Apr 2021 11:37:11: 21000000 INFO @ Sat, 03 Apr 2021 11:37:12: 30000000 INFO @ Sat, 03 Apr 2021 11:37:17: 26000000 INFO @ Sat, 03 Apr 2021 11:37:19: 22000000 INFO @ Sat, 03 Apr 2021 11:37:19: 31000000 INFO @ Sat, 03 Apr 2021 11:37:24: 27000000 INFO @ Sat, 03 Apr 2021 11:37:26: 23000000 INFO @ Sat, 03 Apr 2021 11:37:26: 32000000 INFO @ Sat, 03 Apr 2021 11:37:32: 28000000 INFO @ Sat, 03 Apr 2021 11:37:33: 24000000 INFO @ Sat, 03 Apr 2021 11:37:33: 33000000 INFO @ Sat, 03 Apr 2021 11:37:39: 29000000 INFO @ Sat, 03 Apr 2021 11:37:41: 34000000 INFO @ Sat, 03 Apr 2021 11:37:41: 25000000 INFO @ Sat, 03 Apr 2021 11:37:46: 30000000 INFO @ Sat, 03 Apr 2021 11:37:48: 35000000 INFO @ Sat, 03 Apr 2021 11:37:48: 26000000 INFO @ Sat, 03 Apr 2021 11:37:53: 31000000 INFO @ Sat, 03 Apr 2021 11:37:55: 36000000 INFO @ Sat, 03 Apr 2021 11:37:55: 27000000 INFO @ Sat, 03 Apr 2021 11:38:00: 32000000 INFO @ Sat, 03 Apr 2021 11:38:02: 28000000 INFO @ Sat, 03 Apr 2021 11:38:02: 37000000 INFO @ Sat, 03 Apr 2021 11:38:07: 33000000 INFO @ Sat, 03 Apr 2021 11:38:09: 29000000 INFO @ Sat, 03 Apr 2021 11:38:09: 38000000 INFO @ Sat, 03 Apr 2021 11:38:14: 34000000 INFO @ Sat, 03 Apr 2021 11:38:16: 30000000 INFO @ Sat, 03 Apr 2021 11:38:16: 39000000 INFO @ Sat, 03 Apr 2021 11:38:21: 35000000 INFO @ Sat, 03 Apr 2021 11:38:23: 31000000 INFO @ Sat, 03 Apr 2021 11:38:23: 40000000 INFO @ Sat, 03 Apr 2021 11:38:28: 36000000 INFO @ Sat, 03 Apr 2021 11:38:30: 32000000 INFO @ Sat, 03 Apr 2021 11:38:30: 41000000 INFO @ Sat, 03 Apr 2021 11:38:35: 37000000 INFO @ Sat, 03 Apr 2021 11:38:37: 33000000 INFO @ Sat, 03 Apr 2021 11:38:37: 42000000 INFO @ Sat, 03 Apr 2021 11:38:42: 38000000 INFO @ Sat, 03 Apr 2021 11:38:44: 34000000 INFO @ Sat, 03 Apr 2021 11:38:45: 43000000 INFO @ Sat, 03 Apr 2021 11:38:49: 39000000 INFO @ Sat, 03 Apr 2021 11:38:51: 35000000 INFO @ Sat, 03 Apr 2021 11:38:52: 44000000 INFO @ Sat, 03 Apr 2021 11:38:56: 40000000 INFO @ Sat, 03 Apr 2021 11:38:58: 36000000 INFO @ Sat, 03 Apr 2021 11:38:59: 45000000 INFO @ Sat, 03 Apr 2021 11:39:03: 41000000 INFO @ Sat, 03 Apr 2021 11:39:05: 37000000 INFO @ Sat, 03 Apr 2021 11:39:06: 46000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 11:39:10: 42000000 INFO @ Sat, 03 Apr 2021 11:39:12: 38000000 INFO @ Sat, 03 Apr 2021 11:39:13: 47000000 INFO @ Sat, 03 Apr 2021 11:39:18: 43000000 INFO @ Sat, 03 Apr 2021 11:39:20: 39000000 INFO @ Sat, 03 Apr 2021 11:39:21: 48000000 INFO @ Sat, 03 Apr 2021 11:39:25: 44000000 INFO @ Sat, 03 Apr 2021 11:39:27: 40000000 INFO @ Sat, 03 Apr 2021 11:39:28: 49000000 INFO @ Sat, 03 Apr 2021 11:39:32: 45000000 INFO @ Sat, 03 Apr 2021 11:39:34: 41000000 INFO @ Sat, 03 Apr 2021 11:39:35: 50000000 INFO @ Sat, 03 Apr 2021 11:39:39: 46000000 INFO @ Sat, 03 Apr 2021 11:39:42: 42000000 INFO @ Sat, 03 Apr 2021 11:39:42: 51000000 INFO @ Sat, 03 Apr 2021 11:39:46: 47000000 INFO @ Sat, 03 Apr 2021 11:39:49: 43000000 INFO @ Sat, 03 Apr 2021 11:39:50: 52000000 INFO @ Sat, 03 Apr 2021 11:39:54: 48000000 INFO @ Sat, 03 Apr 2021 11:39:56: 44000000 INFO @ Sat, 03 Apr 2021 11:39:57: 53000000 INFO @ Sat, 03 Apr 2021 11:40:01: 49000000 INFO @ Sat, 03 Apr 2021 11:40:03: #1 tag size is determined as 151 bps INFO @ Sat, 03 Apr 2021 11:40:03: #1 tag size = 151 INFO @ Sat, 03 Apr 2021 11:40:03: #1 total tags in treatment: 19334173 INFO @ Sat, 03 Apr 2021 11:40:03: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 11:40:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 11:40:03: #1 tags after filtering in treatment: 15357829 INFO @ Sat, 03 Apr 2021 11:40:03: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 03 Apr 2021 11:40:03: #1 finished! INFO @ Sat, 03 Apr 2021 11:40:03: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 11:40:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 11:40:04: 45000000 INFO @ Sat, 03 Apr 2021 11:40:04: #2 number of paired peaks: 154 WARNING @ Sat, 03 Apr 2021 11:40:04: Fewer paired peaks (154) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 154 pairs to build model! INFO @ Sat, 03 Apr 2021 11:40:04: start model_add_line... INFO @ Sat, 03 Apr 2021 11:40:04: start X-correlation... INFO @ Sat, 03 Apr 2021 11:40:04: end of X-cor INFO @ Sat, 03 Apr 2021 11:40:04: #2 finished! INFO @ Sat, 03 Apr 2021 11:40:04: #2 predicted fragment length is 194 bps INFO @ Sat, 03 Apr 2021 11:40:04: #2 alternative fragment length(s) may be 194 bps INFO @ Sat, 03 Apr 2021 11:40:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.05_model.r WARNING @ Sat, 03 Apr 2021 11:40:04: #2 Since the d (194) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 11:40:04: #2 You may need to consider one of the other alternative d(s): 194 WARNING @ Sat, 03 Apr 2021 11:40:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 11:40:04: #3 Call peaks... INFO @ Sat, 03 Apr 2021 11:40:04: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 11:40:09: 50000000 INFO @ Sat, 03 Apr 2021 11:40:11: 46000000 INFO @ Sat, 03 Apr 2021 11:40:16: 51000000 INFO @ Sat, 03 Apr 2021 11:40:18: 47000000 INFO @ Sat, 03 Apr 2021 11:40:23: 52000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 11:40:25: 48000000 INFO @ Sat, 03 Apr 2021 11:40:30: 53000000 INFO @ Sat, 03 Apr 2021 11:40:33: 49000000 INFO @ Sat, 03 Apr 2021 11:40:36: #1 tag size is determined as 151 bps INFO @ Sat, 03 Apr 2021 11:40:36: #1 tag size = 151 INFO @ Sat, 03 Apr 2021 11:40:36: #1 total tags in treatment: 19334173 INFO @ Sat, 03 Apr 2021 11:40:36: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 11:40:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 11:40:36: #1 tags after filtering in treatment: 15357829 INFO @ Sat, 03 Apr 2021 11:40:36: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 03 Apr 2021 11:40:36: #1 finished! INFO @ Sat, 03 Apr 2021 11:40:36: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 11:40:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 11:40:37: #2 number of paired peaks: 154 WARNING @ Sat, 03 Apr 2021 11:40:37: Fewer paired peaks (154) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 154 pairs to build model! INFO @ Sat, 03 Apr 2021 11:40:37: start model_add_line... INFO @ Sat, 03 Apr 2021 11:40:37: start X-correlation... INFO @ Sat, 03 Apr 2021 11:40:37: end of X-cor INFO @ Sat, 03 Apr 2021 11:40:37: #2 finished! INFO @ Sat, 03 Apr 2021 11:40:37: #2 predicted fragment length is 194 bps INFO @ Sat, 03 Apr 2021 11:40:37: #2 alternative fragment length(s) may be 194 bps INFO @ Sat, 03 Apr 2021 11:40:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.10_model.r WARNING @ Sat, 03 Apr 2021 11:40:37: #2 Since the d (194) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 11:40:37: #2 You may need to consider one of the other alternative d(s): 194 WARNING @ Sat, 03 Apr 2021 11:40:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 11:40:37: #3 Call peaks... INFO @ Sat, 03 Apr 2021 11:40:37: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 11:40:39: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 11:40:40: 50000000 INFO @ Sat, 03 Apr 2021 11:40:47: 51000000 INFO @ Sat, 03 Apr 2021 11:40:54: 52000000 INFO @ Sat, 03 Apr 2021 11:40:56: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.05_peaks.xls INFO @ Sat, 03 Apr 2021 11:40:56: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 11:40:56: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.05_summits.bed INFO @ Sat, 03 Apr 2021 11:40:56: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (6024 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 11:41:00: 53000000 INFO @ Sat, 03 Apr 2021 11:41:05: #1 tag size is determined as 151 bps INFO @ Sat, 03 Apr 2021 11:41:05: #1 tag size = 151 INFO @ Sat, 03 Apr 2021 11:41:05: #1 total tags in treatment: 19334173 INFO @ Sat, 03 Apr 2021 11:41:05: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 11:41:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 11:41:06: #1 tags after filtering in treatment: 15357829 INFO @ Sat, 03 Apr 2021 11:41:06: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 03 Apr 2021 11:41:06: #1 finished! INFO @ Sat, 03 Apr 2021 11:41:06: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 11:41:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 11:41:07: #2 number of paired peaks: 154 WARNING @ Sat, 03 Apr 2021 11:41:07: Fewer paired peaks (154) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 154 pairs to build model! INFO @ Sat, 03 Apr 2021 11:41:07: start model_add_line... INFO @ Sat, 03 Apr 2021 11:41:07: start X-correlation... INFO @ Sat, 03 Apr 2021 11:41:07: end of X-cor INFO @ Sat, 03 Apr 2021 11:41:07: #2 finished! INFO @ Sat, 03 Apr 2021 11:41:07: #2 predicted fragment length is 194 bps INFO @ Sat, 03 Apr 2021 11:41:07: #2 alternative fragment length(s) may be 194 bps INFO @ Sat, 03 Apr 2021 11:41:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.20_model.r WARNING @ Sat, 03 Apr 2021 11:41:07: #2 Since the d (194) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 11:41:07: #2 You may need to consider one of the other alternative d(s): 194 WARNING @ Sat, 03 Apr 2021 11:41:07: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 11:41:07: #3 Call peaks... INFO @ Sat, 03 Apr 2021 11:41:07: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 11:41:11: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 11:41:29: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.10_peaks.xls INFO @ Sat, 03 Apr 2021 11:41:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 11:41:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.10_summits.bed INFO @ Sat, 03 Apr 2021 11:41:29: Done! pass1 - making usageList (14 chroms): 0 millis pass2 - checking and writing primary data (1885 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 11:41:41: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 11:41:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.20_peaks.xls INFO @ Sat, 03 Apr 2021 11:41:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 11:41:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7572777/SRX7572777.20_summits.bed INFO @ Sat, 03 Apr 2021 11:41:58: Done! pass1 - making usageList (10 chroms): 0 millis pass2 - checking and writing primary data (346 records, 4 fields): 2 millis CompletedMACS2peakCalling