Job ID = 6626760 SRX = SRX7569436 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 17765478 spots for SRR10901258/SRR10901258.sra Written 17765478 spots for SRR10901258/SRR10901258.sra fastq に変換しました。 bowtie でマッピング中... Your job 6627018 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:37 17765478 reads; of these: 17765478 (100.00%) were unpaired; of these: 535644 (3.02%) aligned 0 times 14657892 (82.51%) aligned exactly 1 time 2571942 (14.48%) aligned >1 times 96.98% overall alignment rate Time searching: 00:04:37 Overall time: 00:04:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2660140 / 17229834 = 0.1544 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:47:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:47:03: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:47:03: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:47:07: 1000000 INFO @ Tue, 14 Jul 2020 08:47:12: 2000000 INFO @ Tue, 14 Jul 2020 08:47:17: 3000000 INFO @ Tue, 14 Jul 2020 08:47:22: 4000000 INFO @ Tue, 14 Jul 2020 08:47:26: 5000000 INFO @ Tue, 14 Jul 2020 08:47:31: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:47:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:47:33: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:47:33: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:47:36: 7000000 INFO @ Tue, 14 Jul 2020 08:47:38: 1000000 INFO @ Tue, 14 Jul 2020 08:47:41: 8000000 INFO @ Tue, 14 Jul 2020 08:47:43: 2000000 INFO @ Tue, 14 Jul 2020 08:47:46: 9000000 INFO @ Tue, 14 Jul 2020 08:47:48: 3000000 INFO @ Tue, 14 Jul 2020 08:47:50: 10000000 INFO @ Tue, 14 Jul 2020 08:47:52: 4000000 INFO @ Tue, 14 Jul 2020 08:47:55: 11000000 INFO @ Tue, 14 Jul 2020 08:47:57: 5000000 INFO @ Tue, 14 Jul 2020 08:48:01: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:48:02: 6000000 INFO @ Tue, 14 Jul 2020 08:48:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:48:03: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:48:03: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:48:06: 13000000 INFO @ Tue, 14 Jul 2020 08:48:07: 7000000 INFO @ Tue, 14 Jul 2020 08:48:09: 1000000 INFO @ Tue, 14 Jul 2020 08:48:11: 14000000 INFO @ Tue, 14 Jul 2020 08:48:12: 8000000 INFO @ Tue, 14 Jul 2020 08:48:14: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:48:14: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:48:14: #1 total tags in treatment: 14569694 INFO @ Tue, 14 Jul 2020 08:48:14: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:48:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:48:14: #1 tags after filtering in treatment: 14569694 INFO @ Tue, 14 Jul 2020 08:48:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:48:14: #1 finished! INFO @ Tue, 14 Jul 2020 08:48:14: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:48:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:48:14: 2000000 INFO @ Tue, 14 Jul 2020 08:48:15: #2 number of paired peaks: 40 WARNING @ Tue, 14 Jul 2020 08:48:15: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:48:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:48:17: 9000000 INFO @ Tue, 14 Jul 2020 08:48:20: 3000000 INFO @ Tue, 14 Jul 2020 08:48:22: 10000000 INFO @ Tue, 14 Jul 2020 08:48:26: 4000000 INFO @ Tue, 14 Jul 2020 08:48:28: 11000000 INFO @ Tue, 14 Jul 2020 08:48:31: 5000000 INFO @ Tue, 14 Jul 2020 08:48:33: 12000000 INFO @ Tue, 14 Jul 2020 08:48:37: 6000000 INFO @ Tue, 14 Jul 2020 08:48:38: 13000000 INFO @ Tue, 14 Jul 2020 08:48:43: 7000000 INFO @ Tue, 14 Jul 2020 08:48:43: 14000000 INFO @ Tue, 14 Jul 2020 08:48:46: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:48:46: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:48:46: #1 total tags in treatment: 14569694 INFO @ Tue, 14 Jul 2020 08:48:46: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:48:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:48:46: #1 tags after filtering in treatment: 14569694 INFO @ Tue, 14 Jul 2020 08:48:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:48:46: #1 finished! INFO @ Tue, 14 Jul 2020 08:48:46: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:48:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:48:47: #2 number of paired peaks: 40 WARNING @ Tue, 14 Jul 2020 08:48:47: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:48:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:48:48: 8000000 INFO @ Tue, 14 Jul 2020 08:48:54: 9000000 INFO @ Tue, 14 Jul 2020 08:48:59: 10000000 INFO @ Tue, 14 Jul 2020 08:49:05: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:49:11: 12000000 INFO @ Tue, 14 Jul 2020 08:49:17: 13000000 INFO @ Tue, 14 Jul 2020 08:49:22: 14000000 INFO @ Tue, 14 Jul 2020 08:49:25: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:49:25: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:49:25: #1 total tags in treatment: 14569694 INFO @ Tue, 14 Jul 2020 08:49:25: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:49:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:49:26: #1 tags after filtering in treatment: 14569694 INFO @ Tue, 14 Jul 2020 08:49:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:49:26: #1 finished! INFO @ Tue, 14 Jul 2020 08:49:26: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:49:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:49:27: #2 number of paired peaks: 40 WARNING @ Tue, 14 Jul 2020 08:49:27: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:49:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7569436/SRX7569436.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。