Job ID = 14166956 SRX = SRX7541121 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 21367074 spots for SRR10871149/SRR10871149.sra Written 21367074 spots for SRR10871149/SRR10871149.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167275 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:12 21367074 reads; of these: 21367074 (100.00%) were unpaired; of these: 1316671 (6.16%) aligned 0 times 17400238 (81.43%) aligned exactly 1 time 2650165 (12.40%) aligned >1 times 93.84% overall alignment rate Time searching: 00:06:12 Overall time: 00:06:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7823961 / 20050403 = 0.3902 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:18:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:18:06: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:18:06: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:18:17: 1000000 INFO @ Fri, 10 Dec 2021 08:18:29: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:18:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:18:35: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:18:35: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:18:42: 3000000 INFO @ Fri, 10 Dec 2021 08:18:47: 1000000 INFO @ Fri, 10 Dec 2021 08:18:55: 4000000 INFO @ Fri, 10 Dec 2021 08:19:00: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 08:19:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 08:19:05: #1 read tag files... INFO @ Fri, 10 Dec 2021 08:19:05: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 08:19:07: 5000000 INFO @ Fri, 10 Dec 2021 08:19:13: 3000000 INFO @ Fri, 10 Dec 2021 08:19:14: 1000000 INFO @ Fri, 10 Dec 2021 08:19:20: 6000000 INFO @ Fri, 10 Dec 2021 08:19:24: 2000000 INFO @ Fri, 10 Dec 2021 08:19:26: 4000000 INFO @ Fri, 10 Dec 2021 08:19:33: 7000000 INFO @ Fri, 10 Dec 2021 08:19:34: 3000000 INFO @ Fri, 10 Dec 2021 08:19:39: 5000000 INFO @ Fri, 10 Dec 2021 08:19:43: 4000000 INFO @ Fri, 10 Dec 2021 08:19:45: 8000000 INFO @ Fri, 10 Dec 2021 08:19:52: 6000000 INFO @ Fri, 10 Dec 2021 08:19:52: 5000000 INFO @ Fri, 10 Dec 2021 08:19:58: 9000000 INFO @ Fri, 10 Dec 2021 08:20:02: 6000000 INFO @ Fri, 10 Dec 2021 08:20:05: 7000000 INFO @ Fri, 10 Dec 2021 08:20:11: 7000000 INFO @ Fri, 10 Dec 2021 08:20:11: 10000000 INFO @ Fri, 10 Dec 2021 08:20:17: 8000000 INFO @ Fri, 10 Dec 2021 08:20:20: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 08:20:23: 11000000 INFO @ Fri, 10 Dec 2021 08:20:29: 9000000 INFO @ Fri, 10 Dec 2021 08:20:29: 9000000 INFO @ Fri, 10 Dec 2021 08:20:36: 12000000 INFO @ Fri, 10 Dec 2021 08:20:38: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 08:20:38: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 08:20:38: #1 total tags in treatment: 12226442 INFO @ Fri, 10 Dec 2021 08:20:38: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:20:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:20:39: 10000000 INFO @ Fri, 10 Dec 2021 08:20:39: #1 tags after filtering in treatment: 12226442 INFO @ Fri, 10 Dec 2021 08:20:39: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:20:39: #1 finished! INFO @ Fri, 10 Dec 2021 08:20:39: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:20:39: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:20:40: #2 number of paired peaks: 5578 INFO @ Fri, 10 Dec 2021 08:20:40: start model_add_line... INFO @ Fri, 10 Dec 2021 08:20:41: start X-correlation... INFO @ Fri, 10 Dec 2021 08:20:41: end of X-cor INFO @ Fri, 10 Dec 2021 08:20:41: #2 finished! INFO @ Fri, 10 Dec 2021 08:20:41: #2 predicted fragment length is 233 bps INFO @ Fri, 10 Dec 2021 08:20:41: #2 alternative fragment length(s) may be 233 bps INFO @ Fri, 10 Dec 2021 08:20:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.05_model.r INFO @ Fri, 10 Dec 2021 08:20:41: #3 Call peaks... INFO @ Fri, 10 Dec 2021 08:20:41: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 08:20:41: 10000000 INFO @ Fri, 10 Dec 2021 08:20:48: 11000000 INFO @ Fri, 10 Dec 2021 08:20:52: 11000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 08:20:58: 12000000 INFO @ Fri, 10 Dec 2021 08:21:00: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 08:21:00: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 08:21:00: #1 total tags in treatment: 12226442 INFO @ Fri, 10 Dec 2021 08:21:00: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:21:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:21:00: #1 tags after filtering in treatment: 12226442 INFO @ Fri, 10 Dec 2021 08:21:00: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:21:00: #1 finished! INFO @ Fri, 10 Dec 2021 08:21:00: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:21:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:21:02: #2 number of paired peaks: 5578 INFO @ Fri, 10 Dec 2021 08:21:02: start model_add_line... INFO @ Fri, 10 Dec 2021 08:21:02: start X-correlation... INFO @ Fri, 10 Dec 2021 08:21:02: end of X-cor INFO @ Fri, 10 Dec 2021 08:21:02: #2 finished! INFO @ Fri, 10 Dec 2021 08:21:02: #2 predicted fragment length is 233 bps INFO @ Fri, 10 Dec 2021 08:21:02: #2 alternative fragment length(s) may be 233 bps INFO @ Fri, 10 Dec 2021 08:21:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.20_model.r INFO @ Fri, 10 Dec 2021 08:21:02: #3 Call peaks... INFO @ Fri, 10 Dec 2021 08:21:02: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 08:21:03: 12000000 INFO @ Fri, 10 Dec 2021 08:21:05: #1 tag size is determined as 50 bps INFO @ Fri, 10 Dec 2021 08:21:05: #1 tag size = 50 INFO @ Fri, 10 Dec 2021 08:21:05: #1 total tags in treatment: 12226442 INFO @ Fri, 10 Dec 2021 08:21:05: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 08:21:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 08:21:06: #1 tags after filtering in treatment: 12226442 INFO @ Fri, 10 Dec 2021 08:21:06: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Dec 2021 08:21:06: #1 finished! INFO @ Fri, 10 Dec 2021 08:21:06: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 08:21:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 08:21:07: #2 number of paired peaks: 5578 INFO @ Fri, 10 Dec 2021 08:21:07: start model_add_line... INFO @ Fri, 10 Dec 2021 08:21:07: start X-correlation... INFO @ Fri, 10 Dec 2021 08:21:07: end of X-cor INFO @ Fri, 10 Dec 2021 08:21:07: #2 finished! INFO @ Fri, 10 Dec 2021 08:21:07: #2 predicted fragment length is 233 bps INFO @ Fri, 10 Dec 2021 08:21:07: #2 alternative fragment length(s) may be 233 bps INFO @ Fri, 10 Dec 2021 08:21:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.10_model.r INFO @ Fri, 10 Dec 2021 08:21:07: #3 Call peaks... INFO @ Fri, 10 Dec 2021 08:21:07: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 08:21:23: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 08:21:40: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.05_peaks.xls INFO @ Fri, 10 Dec 2021 08:21:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 08:21:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.05_summits.bed INFO @ Fri, 10 Dec 2021 08:21:40: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (8260 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:21:44: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 08:21:49: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 08:22:01: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.20_peaks.xls INFO @ Fri, 10 Dec 2021 08:22:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 08:22:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.20_summits.bed INFO @ Fri, 10 Dec 2021 08:22:01: Done! pass1 - making usageList (14 chroms): 3 millis pass2 - checking and writing primary data (5583 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 08:22:07: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.10_peaks.xls INFO @ Fri, 10 Dec 2021 08:22:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 08:22:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7541121/SRX7541121.10_summits.bed INFO @ Fri, 10 Dec 2021 08:22:07: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (6952 records, 4 fields): 11 millis CompletedMACS2peakCalling