Job ID = 6528484 SRX = SRX750084 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:11:21 prefetch.2.10.7: 1) Downloading 'SRR1638780'... 2020-06-29T15:11:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:16:54 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:16:54 prefetch.2.10.7: 1) 'SRR1638780' was downloaded successfully Read 46690287 spots for SRR1638780/SRR1638780.sra Written 46690287 spots for SRR1638780/SRR1638780.sra 2020-06-29T15:19:22 prefetch.2.10.7: 1) Downloading 'SRR1638781'... 2020-06-29T15:19:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:24:04 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:24:04 prefetch.2.10.7: 1) 'SRR1638781' was downloaded successfully Read 38238828 spots for SRR1638781/SRR1638781.sra Written 38238828 spots for SRR1638781/SRR1638781.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:37:01 84929115 reads; of these: 84929115 (100.00%) were unpaired; of these: 2354619 (2.77%) aligned 0 times 54582333 (64.27%) aligned exactly 1 time 27992163 (32.96%) aligned >1 times 97.23% overall alignment rate Time searching: 00:37:01 Overall time: 00:37:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 49547346 / 82574496 = 0.6000 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:47:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:47:06: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:47:06: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:47:12: 1000000 INFO @ Tue, 30 Jun 2020 01:47:18: 2000000 INFO @ Tue, 30 Jun 2020 01:47:24: 3000000 INFO @ Tue, 30 Jun 2020 01:47:30: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:47:36: 5000000 INFO @ Tue, 30 Jun 2020 01:47:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:47:36: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:47:36: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:47:42: 6000000 INFO @ Tue, 30 Jun 2020 01:47:44: 1000000 INFO @ Tue, 30 Jun 2020 01:47:49: 7000000 INFO @ Tue, 30 Jun 2020 01:47:52: 2000000 INFO @ Tue, 30 Jun 2020 01:47:56: 8000000 INFO @ Tue, 30 Jun 2020 01:48:00: 3000000 INFO @ Tue, 30 Jun 2020 01:48:03: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:48:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:48:06: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:48:06: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:48:07: 4000000 INFO @ Tue, 30 Jun 2020 01:48:09: 10000000 INFO @ Tue, 30 Jun 2020 01:48:14: 1000000 INFO @ Tue, 30 Jun 2020 01:48:15: 5000000 INFO @ Tue, 30 Jun 2020 01:48:16: 11000000 INFO @ Tue, 30 Jun 2020 01:48:22: 2000000 INFO @ Tue, 30 Jun 2020 01:48:22: 6000000 INFO @ Tue, 30 Jun 2020 01:48:22: 12000000 INFO @ Tue, 30 Jun 2020 01:48:29: 13000000 INFO @ Tue, 30 Jun 2020 01:48:29: 3000000 INFO @ Tue, 30 Jun 2020 01:48:30: 7000000 INFO @ Tue, 30 Jun 2020 01:48:36: 14000000 INFO @ Tue, 30 Jun 2020 01:48:37: 4000000 INFO @ Tue, 30 Jun 2020 01:48:37: 8000000 INFO @ Tue, 30 Jun 2020 01:48:42: 15000000 INFO @ Tue, 30 Jun 2020 01:48:44: 5000000 INFO @ Tue, 30 Jun 2020 01:48:45: 9000000 INFO @ Tue, 30 Jun 2020 01:48:49: 16000000 INFO @ Tue, 30 Jun 2020 01:48:51: 6000000 INFO @ Tue, 30 Jun 2020 01:48:52: 10000000 INFO @ Tue, 30 Jun 2020 01:48:56: 17000000 INFO @ Tue, 30 Jun 2020 01:48:58: 7000000 INFO @ Tue, 30 Jun 2020 01:48:59: 11000000 INFO @ Tue, 30 Jun 2020 01:49:02: 18000000 INFO @ Tue, 30 Jun 2020 01:49:06: 8000000 INFO @ Tue, 30 Jun 2020 01:49:06: 12000000 INFO @ Tue, 30 Jun 2020 01:49:09: 19000000 INFO @ Tue, 30 Jun 2020 01:49:13: 9000000 INFO @ Tue, 30 Jun 2020 01:49:14: 13000000 INFO @ Tue, 30 Jun 2020 01:49:16: 20000000 INFO @ Tue, 30 Jun 2020 01:49:20: 10000000 INFO @ Tue, 30 Jun 2020 01:49:21: 14000000 INFO @ Tue, 30 Jun 2020 01:49:23: 21000000 INFO @ Tue, 30 Jun 2020 01:49:28: 11000000 INFO @ Tue, 30 Jun 2020 01:49:28: 15000000 INFO @ Tue, 30 Jun 2020 01:49:29: 22000000 INFO @ Tue, 30 Jun 2020 01:49:35: 12000000 INFO @ Tue, 30 Jun 2020 01:49:35: 16000000 INFO @ Tue, 30 Jun 2020 01:49:36: 23000000 INFO @ Tue, 30 Jun 2020 01:49:43: 17000000 INFO @ Tue, 30 Jun 2020 01:49:43: 13000000 INFO @ Tue, 30 Jun 2020 01:49:43: 24000000 INFO @ Tue, 30 Jun 2020 01:49:50: 18000000 INFO @ Tue, 30 Jun 2020 01:49:50: 25000000 INFO @ Tue, 30 Jun 2020 01:49:50: 14000000 INFO @ Tue, 30 Jun 2020 01:49:56: 26000000 INFO @ Tue, 30 Jun 2020 01:49:57: 19000000 INFO @ Tue, 30 Jun 2020 01:49:58: 15000000 INFO @ Tue, 30 Jun 2020 01:50:03: 27000000 INFO @ Tue, 30 Jun 2020 01:50:04: 20000000 INFO @ Tue, 30 Jun 2020 01:50:05: 16000000 INFO @ Tue, 30 Jun 2020 01:50:10: 28000000 INFO @ Tue, 30 Jun 2020 01:50:11: 21000000 INFO @ Tue, 30 Jun 2020 01:50:13: 17000000 INFO @ Tue, 30 Jun 2020 01:50:17: 29000000 INFO @ Tue, 30 Jun 2020 01:50:18: 22000000 INFO @ Tue, 30 Jun 2020 01:50:20: 18000000 INFO @ Tue, 30 Jun 2020 01:50:24: 30000000 INFO @ Tue, 30 Jun 2020 01:50:25: 23000000 INFO @ Tue, 30 Jun 2020 01:50:28: 19000000 INFO @ Tue, 30 Jun 2020 01:50:31: 31000000 INFO @ Tue, 30 Jun 2020 01:50:32: 24000000 INFO @ Tue, 30 Jun 2020 01:50:35: 20000000 INFO @ Tue, 30 Jun 2020 01:50:37: 32000000 INFO @ Tue, 30 Jun 2020 01:50:40: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:50:43: 21000000 INFO @ Tue, 30 Jun 2020 01:50:44: 33000000 INFO @ Tue, 30 Jun 2020 01:50:45: #1 tag size is determined as 67 bps INFO @ Tue, 30 Jun 2020 01:50:45: #1 tag size = 67 INFO @ Tue, 30 Jun 2020 01:50:45: #1 total tags in treatment: 33027150 INFO @ Tue, 30 Jun 2020 01:50:45: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:50:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:50:45: #1 tags after filtering in treatment: 33027150 INFO @ Tue, 30 Jun 2020 01:50:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:50:45: #1 finished! INFO @ Tue, 30 Jun 2020 01:50:45: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:50:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:50:47: 26000000 INFO @ Tue, 30 Jun 2020 01:50:47: #2 number of paired peaks: 65 WARNING @ Tue, 30 Jun 2020 01:50:47: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:50:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:50:50: 22000000 INFO @ Tue, 30 Jun 2020 01:50:54: 27000000 INFO @ Tue, 30 Jun 2020 01:50:58: 23000000 INFO @ Tue, 30 Jun 2020 01:51:01: 28000000 INFO @ Tue, 30 Jun 2020 01:51:05: 24000000 INFO @ Tue, 30 Jun 2020 01:51:08: 29000000 INFO @ Tue, 30 Jun 2020 01:51:12: 25000000 INFO @ Tue, 30 Jun 2020 01:51:15: 30000000 INFO @ Tue, 30 Jun 2020 01:51:20: 26000000 INFO @ Tue, 30 Jun 2020 01:51:22: 31000000 INFO @ Tue, 30 Jun 2020 01:51:27: 27000000 INFO @ Tue, 30 Jun 2020 01:51:29: 32000000 INFO @ Tue, 30 Jun 2020 01:51:35: 28000000 INFO @ Tue, 30 Jun 2020 01:51:36: 33000000 INFO @ Tue, 30 Jun 2020 01:51:37: #1 tag size is determined as 67 bps INFO @ Tue, 30 Jun 2020 01:51:37: #1 tag size = 67 INFO @ Tue, 30 Jun 2020 01:51:37: #1 total tags in treatment: 33027150 INFO @ Tue, 30 Jun 2020 01:51:37: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:51:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:51:37: #1 tags after filtering in treatment: 33027150 INFO @ Tue, 30 Jun 2020 01:51:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:51:37: #1 finished! INFO @ Tue, 30 Jun 2020 01:51:37: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:51:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:51:39: #2 number of paired peaks: 65 WARNING @ Tue, 30 Jun 2020 01:51:39: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:51:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:51:42: 29000000 INFO @ Tue, 30 Jun 2020 01:51:49: 30000000 BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 01:51:56: 31000000 INFO @ Tue, 30 Jun 2020 01:52:03: 32000000 INFO @ Tue, 30 Jun 2020 01:52:10: 33000000 INFO @ Tue, 30 Jun 2020 01:52:10: #1 tag size is determined as 67 bps INFO @ Tue, 30 Jun 2020 01:52:10: #1 tag size = 67 INFO @ Tue, 30 Jun 2020 01:52:10: #1 total tags in treatment: 33027150 INFO @ Tue, 30 Jun 2020 01:52:10: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:52:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:52:11: #1 tags after filtering in treatment: 33027150 INFO @ Tue, 30 Jun 2020 01:52:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:52:11: #1 finished! INFO @ Tue, 30 Jun 2020 01:52:11: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:52:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:52:13: #2 number of paired peaks: 65 WARNING @ Tue, 30 Jun 2020 01:52:13: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:52:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750084/SRX750084.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling