Job ID = 6498754 SRX = SRX750077 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T00:07:19 prefetch.2.10.7: 1) Downloading 'SRR1638766'... 2020-06-26T00:07:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:09:44 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:09:44 prefetch.2.10.7: 1) 'SRR1638766' was downloaded successfully Read 20750624 spots for SRR1638766/SRR1638766.sra Written 20750624 spots for SRR1638766/SRR1638766.sra 2020-06-26T00:11:13 prefetch.2.10.7: 1) Downloading 'SRR1638767'... 2020-06-26T00:11:13 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:14:50 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:14:50 prefetch.2.10.7: 1) 'SRR1638767' was downloaded successfully Read 32832782 spots for SRR1638767/SRR1638767.sra Written 32832782 spots for SRR1638767/SRR1638767.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:26:51 53583406 reads; of these: 53583406 (100.00%) were unpaired; of these: 1689736 (3.15%) aligned 0 times 35494956 (66.24%) aligned exactly 1 time 16398714 (30.60%) aligned >1 times 96.85% overall alignment rate Time searching: 00:26:52 Overall time: 00:26:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 24479786 / 51893670 = 0.4717 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:58:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:58:34: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:58:34: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:58:44: 1000000 INFO @ Fri, 26 Jun 2020 09:58:53: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:59:03: 3000000 INFO @ Fri, 26 Jun 2020 09:59:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:59:04: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:59:04: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:59:13: 4000000 INFO @ Fri, 26 Jun 2020 09:59:14: 1000000 INFO @ Fri, 26 Jun 2020 09:59:22: 5000000 INFO @ Fri, 26 Jun 2020 09:59:24: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:59:32: 6000000 INFO @ Fri, 26 Jun 2020 09:59:33: 3000000 INFO @ Fri, 26 Jun 2020 09:59:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:59:34: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:59:34: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:59:42: 7000000 INFO @ Fri, 26 Jun 2020 09:59:43: 4000000 INFO @ Fri, 26 Jun 2020 09:59:44: 1000000 INFO @ Fri, 26 Jun 2020 09:59:52: 8000000 INFO @ Fri, 26 Jun 2020 09:59:53: 5000000 INFO @ Fri, 26 Jun 2020 09:59:54: 2000000 INFO @ Fri, 26 Jun 2020 10:00:01: 9000000 INFO @ Fri, 26 Jun 2020 10:00:02: 6000000 INFO @ Fri, 26 Jun 2020 10:00:04: 3000000 INFO @ Fri, 26 Jun 2020 10:00:10: 10000000 INFO @ Fri, 26 Jun 2020 10:00:11: 7000000 INFO @ Fri, 26 Jun 2020 10:00:14: 4000000 INFO @ Fri, 26 Jun 2020 10:00:20: 11000000 INFO @ Fri, 26 Jun 2020 10:00:21: 8000000 INFO @ Fri, 26 Jun 2020 10:00:24: 5000000 INFO @ Fri, 26 Jun 2020 10:00:29: 12000000 INFO @ Fri, 26 Jun 2020 10:00:30: 9000000 INFO @ Fri, 26 Jun 2020 10:00:35: 6000000 INFO @ Fri, 26 Jun 2020 10:00:37: 13000000 INFO @ Fri, 26 Jun 2020 10:00:38: 10000000 INFO @ Fri, 26 Jun 2020 10:00:45: 7000000 INFO @ Fri, 26 Jun 2020 10:00:45: 14000000 INFO @ Fri, 26 Jun 2020 10:00:46: 11000000 INFO @ Fri, 26 Jun 2020 10:00:54: 15000000 INFO @ Fri, 26 Jun 2020 10:00:55: 12000000 INFO @ Fri, 26 Jun 2020 10:00:55: 8000000 INFO @ Fri, 26 Jun 2020 10:01:03: 16000000 INFO @ Fri, 26 Jun 2020 10:01:03: 13000000 INFO @ Fri, 26 Jun 2020 10:01:06: 9000000 INFO @ Fri, 26 Jun 2020 10:01:11: 17000000 INFO @ Fri, 26 Jun 2020 10:01:12: 14000000 INFO @ Fri, 26 Jun 2020 10:01:16: 10000000 INFO @ Fri, 26 Jun 2020 10:01:20: 15000000 INFO @ Fri, 26 Jun 2020 10:01:20: 18000000 INFO @ Fri, 26 Jun 2020 10:01:26: 11000000 INFO @ Fri, 26 Jun 2020 10:01:29: 19000000 INFO @ Fri, 26 Jun 2020 10:01:29: 16000000 INFO @ Fri, 26 Jun 2020 10:01:36: 12000000 INFO @ Fri, 26 Jun 2020 10:01:37: 20000000 INFO @ Fri, 26 Jun 2020 10:01:37: 17000000 INFO @ Fri, 26 Jun 2020 10:01:45: 21000000 INFO @ Fri, 26 Jun 2020 10:01:46: 18000000 INFO @ Fri, 26 Jun 2020 10:01:46: 13000000 INFO @ Fri, 26 Jun 2020 10:01:54: 22000000 INFO @ Fri, 26 Jun 2020 10:01:54: 19000000 INFO @ Fri, 26 Jun 2020 10:01:56: 14000000 INFO @ Fri, 26 Jun 2020 10:02:02: 23000000 INFO @ Fri, 26 Jun 2020 10:02:03: 20000000 INFO @ Fri, 26 Jun 2020 10:02:07: 15000000 INFO @ Fri, 26 Jun 2020 10:02:11: 24000000 INFO @ Fri, 26 Jun 2020 10:02:11: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 10:02:17: 16000000 INFO @ Fri, 26 Jun 2020 10:02:19: 25000000 INFO @ Fri, 26 Jun 2020 10:02:20: 22000000 INFO @ Fri, 26 Jun 2020 10:02:27: 17000000 INFO @ Fri, 26 Jun 2020 10:02:28: 26000000 INFO @ Fri, 26 Jun 2020 10:02:28: 23000000 INFO @ Fri, 26 Jun 2020 10:02:36: 27000000 INFO @ Fri, 26 Jun 2020 10:02:36: 24000000 INFO @ Fri, 26 Jun 2020 10:02:37: 18000000 INFO @ Fri, 26 Jun 2020 10:02:39: #1 tag size is determined as 67 bps INFO @ Fri, 26 Jun 2020 10:02:39: #1 tag size = 67 INFO @ Fri, 26 Jun 2020 10:02:39: #1 total tags in treatment: 27413884 INFO @ Fri, 26 Jun 2020 10:02:39: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 10:02:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 10:02:40: #1 tags after filtering in treatment: 27413884 INFO @ Fri, 26 Jun 2020 10:02:40: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 10:02:40: #1 finished! INFO @ Fri, 26 Jun 2020 10:02:40: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 10:02:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 10:02:42: #2 number of paired peaks: 90 WARNING @ Fri, 26 Jun 2020 10:02:42: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 10:02:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 10:02:44: 25000000 INFO @ Fri, 26 Jun 2020 10:02:47: 19000000 INFO @ Fri, 26 Jun 2020 10:02:52: 26000000 INFO @ Fri, 26 Jun 2020 10:02:57: 20000000 INFO @ Fri, 26 Jun 2020 10:03:00: 27000000 INFO @ Fri, 26 Jun 2020 10:03:03: #1 tag size is determined as 67 bps INFO @ Fri, 26 Jun 2020 10:03:03: #1 tag size = 67 INFO @ Fri, 26 Jun 2020 10:03:03: #1 total tags in treatment: 27413884 INFO @ Fri, 26 Jun 2020 10:03:03: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 10:03:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 10:03:04: #1 tags after filtering in treatment: 27413884 INFO @ Fri, 26 Jun 2020 10:03:04: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 10:03:04: #1 finished! INFO @ Fri, 26 Jun 2020 10:03:04: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 10:03:04: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 10:03:06: #2 number of paired peaks: 90 WARNING @ Fri, 26 Jun 2020 10:03:06: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 10:03:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 10:03:08: 21000000 INFO @ Fri, 26 Jun 2020 10:03:18: 22000000 INFO @ Fri, 26 Jun 2020 10:03:28: 23000000 BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 10:03:39: 24000000 INFO @ Fri, 26 Jun 2020 10:03:49: 25000000 INFO @ Fri, 26 Jun 2020 10:03:59: 26000000 INFO @ Fri, 26 Jun 2020 10:04:09: 27000000 INFO @ Fri, 26 Jun 2020 10:04:13: #1 tag size is determined as 67 bps INFO @ Fri, 26 Jun 2020 10:04:13: #1 tag size = 67 INFO @ Fri, 26 Jun 2020 10:04:13: #1 total tags in treatment: 27413884 INFO @ Fri, 26 Jun 2020 10:04:13: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 10:04:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 10:04:14: #1 tags after filtering in treatment: 27413884 INFO @ Fri, 26 Jun 2020 10:04:14: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 10:04:14: #1 finished! INFO @ Fri, 26 Jun 2020 10:04:14: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 10:04:14: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 10:04:16: #2 number of paired peaks: 90 WARNING @ Fri, 26 Jun 2020 10:04:16: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 10:04:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX750077/SRX750077.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling