Job ID = 14172468 SRX = SRX7434132 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 8208933 spots for SRR10759898/SRR10759898.sra Written 8208933 spots for SRR10759898/SRR10759898.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172896 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:23 8208933 reads; of these: 8208933 (100.00%) were unpaired; of these: 529469 (6.45%) aligned 0 times 6133187 (74.71%) aligned exactly 1 time 1546277 (18.84%) aligned >1 times 93.55% overall alignment rate Time searching: 00:02:23 Overall time: 00:02:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 3375486 / 7679464 = 0.4395 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:37:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:37:05: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:37:05: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:37:12: 1000000 INFO @ Sat, 11 Dec 2021 15:37:18: 2000000 INFO @ Sat, 11 Dec 2021 15:37:24: 3000000 INFO @ Sat, 11 Dec 2021 15:37:30: 4000000 INFO @ Sat, 11 Dec 2021 15:37:32: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 15:37:32: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 15:37:32: #1 total tags in treatment: 4303978 INFO @ Sat, 11 Dec 2021 15:37:32: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:37:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:37:32: #1 tags after filtering in treatment: 4303978 INFO @ Sat, 11 Dec 2021 15:37:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:37:32: #1 finished! INFO @ Sat, 11 Dec 2021 15:37:32: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:37:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:37:33: #2 number of paired peaks: 3680 INFO @ Sat, 11 Dec 2021 15:37:33: start model_add_line... INFO @ Sat, 11 Dec 2021 15:37:33: start X-correlation... INFO @ Sat, 11 Dec 2021 15:37:33: end of X-cor INFO @ Sat, 11 Dec 2021 15:37:33: #2 finished! INFO @ Sat, 11 Dec 2021 15:37:33: #2 predicted fragment length is 231 bps INFO @ Sat, 11 Dec 2021 15:37:33: #2 alternative fragment length(s) may be 231 bps INFO @ Sat, 11 Dec 2021 15:37:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.05_model.r INFO @ Sat, 11 Dec 2021 15:37:33: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:37:33: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:37:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:37:35: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:37:35: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:37:41: 1000000 INFO @ Sat, 11 Dec 2021 15:37:44: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:37:47: 2000000 INFO @ Sat, 11 Dec 2021 15:37:49: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.05_peaks.xls INFO @ Sat, 11 Dec 2021 15:37:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:37:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.05_summits.bed INFO @ Sat, 11 Dec 2021 15:37:49: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (4285 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:37:53: 3000000 INFO @ Sat, 11 Dec 2021 15:37:59: 4000000 INFO @ Sat, 11 Dec 2021 15:38:00: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 15:38:00: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 15:38:00: #1 total tags in treatment: 4303978 INFO @ Sat, 11 Dec 2021 15:38:00: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:38:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:38:00: #1 tags after filtering in treatment: 4303978 INFO @ Sat, 11 Dec 2021 15:38:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:38:00: #1 finished! INFO @ Sat, 11 Dec 2021 15:38:00: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:38:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:38:01: #2 number of paired peaks: 3680 INFO @ Sat, 11 Dec 2021 15:38:01: start model_add_line... INFO @ Sat, 11 Dec 2021 15:38:01: start X-correlation... INFO @ Sat, 11 Dec 2021 15:38:01: end of X-cor INFO @ Sat, 11 Dec 2021 15:38:01: #2 finished! INFO @ Sat, 11 Dec 2021 15:38:01: #2 predicted fragment length is 231 bps INFO @ Sat, 11 Dec 2021 15:38:01: #2 alternative fragment length(s) may be 231 bps INFO @ Sat, 11 Dec 2021 15:38:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.10_model.r INFO @ Sat, 11 Dec 2021 15:38:01: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:38:01: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:38:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:38:05: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:38:05: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:38:11: 1000000 INFO @ Sat, 11 Dec 2021 15:38:11: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:38:16: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.10_peaks.xls INFO @ Sat, 11 Dec 2021 15:38:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:38:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.10_summits.bed INFO @ Sat, 11 Dec 2021 15:38:16: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (3322 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:38:18: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 15:38:24: 3000000 INFO @ Sat, 11 Dec 2021 15:38:30: 4000000 INFO @ Sat, 11 Dec 2021 15:38:32: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 15:38:32: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 15:38:32: #1 total tags in treatment: 4303978 INFO @ Sat, 11 Dec 2021 15:38:32: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:38:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:38:32: #1 tags after filtering in treatment: 4303978 INFO @ Sat, 11 Dec 2021 15:38:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:38:32: #1 finished! INFO @ Sat, 11 Dec 2021 15:38:32: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:38:32: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 15:38:33: #2 number of paired peaks: 3680 INFO @ Sat, 11 Dec 2021 15:38:33: start model_add_line... INFO @ Sat, 11 Dec 2021 15:38:33: start X-correlation... INFO @ Sat, 11 Dec 2021 15:38:33: end of X-cor INFO @ Sat, 11 Dec 2021 15:38:33: #2 finished! INFO @ Sat, 11 Dec 2021 15:38:33: #2 predicted fragment length is 231 bps INFO @ Sat, 11 Dec 2021 15:38:33: #2 alternative fragment length(s) may be 231 bps INFO @ Sat, 11 Dec 2021 15:38:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.20_model.r INFO @ Sat, 11 Dec 2021 15:38:33: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:38:33: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:38:44: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:38:49: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.20_peaks.xls INFO @ Sat, 11 Dec 2021 15:38:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:38:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7434132/SRX7434132.20_summits.bed INFO @ Sat, 11 Dec 2021 15:38:49: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (2573 records, 4 fields): 4 millis CompletedMACS2peakCalling