Job ID = 14172403 SRX = SRX7434125 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 24535218 spots for SRR10759891/SRR10759891.sra Written 24535218 spots for SRR10759891/SRR10759891.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172859 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:28 24535218 reads; of these: 24535218 (100.00%) were unpaired; of these: 8187812 (33.37%) aligned 0 times 11888685 (48.46%) aligned exactly 1 time 4458721 (18.17%) aligned >1 times 66.63% overall alignment rate Time searching: 00:08:28 Overall time: 00:08:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3270171 / 16347406 = 0.2000 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:32:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:32:34: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:32:34: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:32:43: 1000000 INFO @ Sat, 11 Dec 2021 15:32:52: 2000000 INFO @ Sat, 11 Dec 2021 15:33:01: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:33:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:33:04: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:33:04: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:33:10: 4000000 INFO @ Sat, 11 Dec 2021 15:33:13: 1000000 INFO @ Sat, 11 Dec 2021 15:33:19: 5000000 INFO @ Sat, 11 Dec 2021 15:33:22: 2000000 INFO @ Sat, 11 Dec 2021 15:33:28: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:33:32: 3000000 INFO @ Sat, 11 Dec 2021 15:33:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:33:34: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:33:34: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:33:36: 7000000 INFO @ Sat, 11 Dec 2021 15:33:41: 4000000 INFO @ Sat, 11 Dec 2021 15:33:44: 1000000 INFO @ Sat, 11 Dec 2021 15:33:45: 8000000 INFO @ Sat, 11 Dec 2021 15:33:51: 5000000 INFO @ Sat, 11 Dec 2021 15:33:52: 2000000 INFO @ Sat, 11 Dec 2021 15:33:54: 9000000 INFO @ Sat, 11 Dec 2021 15:34:00: 6000000 INFO @ Sat, 11 Dec 2021 15:34:01: 3000000 INFO @ Sat, 11 Dec 2021 15:34:03: 10000000 INFO @ Sat, 11 Dec 2021 15:34:09: 7000000 INFO @ Sat, 11 Dec 2021 15:34:10: 4000000 INFO @ Sat, 11 Dec 2021 15:34:12: 11000000 INFO @ Sat, 11 Dec 2021 15:34:18: 5000000 INFO @ Sat, 11 Dec 2021 15:34:18: 8000000 INFO @ Sat, 11 Dec 2021 15:34:21: 12000000 INFO @ Sat, 11 Dec 2021 15:34:26: 6000000 INFO @ Sat, 11 Dec 2021 15:34:27: 9000000 INFO @ Sat, 11 Dec 2021 15:34:30: 13000000 INFO @ Sat, 11 Dec 2021 15:34:31: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 15:34:31: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 15:34:31: #1 total tags in treatment: 13077235 INFO @ Sat, 11 Dec 2021 15:34:31: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:34:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:34:31: #1 tags after filtering in treatment: 13077235 INFO @ Sat, 11 Dec 2021 15:34:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:34:31: #1 finished! INFO @ Sat, 11 Dec 2021 15:34:31: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:34:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:34:32: #2 number of paired peaks: 1456 INFO @ Sat, 11 Dec 2021 15:34:32: start model_add_line... INFO @ Sat, 11 Dec 2021 15:34:32: start X-correlation... INFO @ Sat, 11 Dec 2021 15:34:33: end of X-cor INFO @ Sat, 11 Dec 2021 15:34:33: #2 finished! INFO @ Sat, 11 Dec 2021 15:34:33: #2 predicted fragment length is 212 bps INFO @ Sat, 11 Dec 2021 15:34:33: #2 alternative fragment length(s) may be 212 bps INFO @ Sat, 11 Dec 2021 15:34:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.05_model.r INFO @ Sat, 11 Dec 2021 15:34:33: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:34:33: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:34:34: 7000000 INFO @ Sat, 11 Dec 2021 15:34:36: 10000000 INFO @ Sat, 11 Dec 2021 15:34:43: 8000000 INFO @ Sat, 11 Dec 2021 15:34:45: 11000000 INFO @ Sat, 11 Dec 2021 15:34:51: 9000000 INFO @ Sat, 11 Dec 2021 15:34:54: 12000000 INFO @ Sat, 11 Dec 2021 15:34:59: 10000000 INFO @ Sat, 11 Dec 2021 15:35:03: 13000000 INFO @ Sat, 11 Dec 2021 15:35:04: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 15:35:04: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 15:35:04: #1 total tags in treatment: 13077235 INFO @ Sat, 11 Dec 2021 15:35:04: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:35:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:35:04: #1 tags after filtering in treatment: 13077235 INFO @ Sat, 11 Dec 2021 15:35:04: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:35:04: #1 finished! INFO @ Sat, 11 Dec 2021 15:35:04: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:35:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:35:05: #2 number of paired peaks: 1456 INFO @ Sat, 11 Dec 2021 15:35:05: start model_add_line... INFO @ Sat, 11 Dec 2021 15:35:06: start X-correlation... INFO @ Sat, 11 Dec 2021 15:35:06: end of X-cor INFO @ Sat, 11 Dec 2021 15:35:06: #2 finished! INFO @ Sat, 11 Dec 2021 15:35:06: #2 predicted fragment length is 212 bps INFO @ Sat, 11 Dec 2021 15:35:06: #2 alternative fragment length(s) may be 212 bps INFO @ Sat, 11 Dec 2021 15:35:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.10_model.r INFO @ Sat, 11 Dec 2021 15:35:06: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:35:06: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:35:07: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 15:35:15: 12000000 INFO @ Sat, 11 Dec 2021 15:35:15: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:35:23: 13000000 INFO @ Sat, 11 Dec 2021 15:35:23: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 15:35:23: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 15:35:23: #1 total tags in treatment: 13077235 INFO @ Sat, 11 Dec 2021 15:35:23: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:35:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:35:24: #1 tags after filtering in treatment: 13077235 INFO @ Sat, 11 Dec 2021 15:35:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 15:35:24: #1 finished! INFO @ Sat, 11 Dec 2021 15:35:24: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:35:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:35:25: #2 number of paired peaks: 1456 INFO @ Sat, 11 Dec 2021 15:35:25: start model_add_line... INFO @ Sat, 11 Dec 2021 15:35:25: start X-correlation... INFO @ Sat, 11 Dec 2021 15:35:25: end of X-cor INFO @ Sat, 11 Dec 2021 15:35:25: #2 finished! INFO @ Sat, 11 Dec 2021 15:35:25: #2 predicted fragment length is 212 bps INFO @ Sat, 11 Dec 2021 15:35:25: #2 alternative fragment length(s) may be 212 bps INFO @ Sat, 11 Dec 2021 15:35:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.20_model.r INFO @ Sat, 11 Dec 2021 15:35:25: #3 Call peaks... INFO @ Sat, 11 Dec 2021 15:35:25: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 15:35:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.05_peaks.xls INFO @ Sat, 11 Dec 2021 15:35:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:35:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.05_summits.bed INFO @ Sat, 11 Dec 2021 15:35:35: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (4923 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:35:48: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 15:36:07: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.10_peaks.xls INFO @ Sat, 11 Dec 2021 15:36:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:36:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.10_summits.bed INFO @ Sat, 11 Dec 2021 15:36:07: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (3493 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:36:09: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 15:36:28: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.20_peaks.xls INFO @ Sat, 11 Dec 2021 15:36:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 15:36:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX7434125/SRX7434125.20_summits.bed INFO @ Sat, 11 Dec 2021 15:36:28: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (2499 records, 4 fields): 6 millis CompletedMACS2peakCalling